##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293839
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5821200
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.534529822029825	39.0	39.0	39.0	38.0	39.0
2	40.327426132068986	41.0	40.0	41.0	39.0	41.0
3	40.34606850821137	41.0	40.0	41.0	39.0	41.0
4	40.348460626674914	41.0	40.0	41.0	39.0	41.0
5	40.336415172129456	41.0	40.0	41.0	39.0	41.0
6	40.31955266955267	41.0	40.0	41.0	39.0	41.0
7	40.353420772349345	41.0	40.0	41.0	39.0	41.0
8	40.338660757232184	41.0	40.0	41.0	39.0	41.0
9	40.34008675187247	41.0	40.0	41.0	39.0	41.0
10-14	40.28337634851921	41.0	40.0	41.0	39.0	41.0
15-19	40.19427221191506	41.0	40.0	41.0	38.0	41.0
20-24	40.07204717240431	41.0	40.0	41.0	38.0	41.0
25-29	39.86738792688793	41.0	40.0	41.0	37.6	41.0
30-34	39.56227166220024	40.6	39.2	41.0	36.2	41.0
35-39	39.10008740465883	40.0	38.0	41.0	35.0	41.0
40-44	38.59941438191438	40.0	36.6	41.0	35.0	41.0
45-49	38.05255638012781	39.0	35.2	41.0	35.0	41.0
50-54	37.46535367278225	37.2	35.0	40.4	35.0	41.0
55-59	36.749504500790216	35.8	35.0	39.0	35.0	41.0
60-64	35.94476819212534	35.0	34.8	37.0	33.6	40.0
65-69	35.54382240775098	35.0	35.0	36.0	33.8	38.8
70-74	35.317480278980284	35.0	35.0	35.6	34.0	37.0
75-79	35.12850481000481	35.0	35.0	35.0	34.0	36.4
80-84	35.04583563526421	35.0	35.0	35.0	34.0	36.0
85-89	34.93254325568612	35.0	34.8	35.0	33.8	36.0
90-94	39.78076697337376	40.6	39.8	40.6	37.8	40.6
95-99	40.09516938076679	41.0	40.0	41.0	38.0	41.0
100-104	40.003652658560426	41.0	40.0	41.0	38.0	41.0
105-109	39.89106476744026	41.0	40.0	41.0	37.6	41.0
110-114	39.72343592402418	41.0	40.0	41.0	36.8	41.0
115-119	39.48276644840817	40.8	39.0	41.0	35.6	41.0
120-124	39.13228436431934	40.0	38.2	41.0	35.0	41.0
125-129	38.214503092374756	39.4	36.6	40.4	34.4	40.6
130-134	38.18129483770161	39.0	35.8	40.8	34.8	41.0
135-139	37.86718076361206	38.6	35.0	41.0	35.0	41.0
140-144	37.219046832021164	36.8	35.0	40.0	35.0	41.0
145-149	36.50805875739051	35.6	35.0	38.6	34.0	41.0
150-154	35.87477881971222	35.0	35.0	36.8	34.0	39.2
155-159	35.37458572706104	35.0	35.0	36.0	34.0	37.8
160-164	35.03339100442163	35.0	35.0	35.0	34.0	36.4
165-169	34.835257834106265	35.0	35.0	35.0	34.0	36.0
170-174	34.731705341937555	35.0	35.0	35.0	34.0	36.0
175-178	34.5797704204778	35.0	34.75	35.0	33.75	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	22.0
30	268.0
31	1962.0
32	9218.0
33	33232.0
34	92555.0
35	242423.0
36	779946.0
37	1939654.0
38	2129040.0
39	583904.0
40	8973.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.56557067271353	26.877379234522092	27.25436336150622	23.30268673125816
2	23.751477358620214	27.19465058750773	26.97797704940562	22.075895004466435
3	21.942657871229297	26.50249089534804	27.902563045420187	23.652288188002473
4	22.469903112760257	25.629234522091664	28.29730983302412	23.603552532123963
5	23.268484161341306	26.13985088985089	26.95844499415928	23.633219954648528
6	23.126382876382877	26.287277537277536	27.00061842918986	23.585721157149727
7	23.32838589981447	26.362622826908545	26.610355253212397	23.698636020064594
8	23.46950800522229	26.554232804232804	26.477822442108156	23.49843674843675
9	23.39698000412286	26.61997526283241	26.585738335738334	23.397306397306398
10-14	23.41876245447674	26.576860441146156	26.43469731326874	23.569679791108364
15-19	23.360509860509858	26.574561945990517	26.51948051948052	23.5454476740191
20-24	23.341043083900225	26.514203257060398	26.61373256373256	23.53102109530681
25-29	23.375785061499347	26.514484985913555	26.45217480931767	23.65755514326943
30-34	23.317103002817287	26.56024531024531	26.552047687761977	23.570603999175425
35-39	23.295093795093795	26.607287157287157	26.522077922077926	23.575541125541125
40-44	23.339122517693948	26.583134061705486	26.47730708444994	23.600436336150622
45-49	23.459997938569366	26.519810348381778	26.407744107744108	23.612447605304748
50-54	23.42790489933347	26.582556861128285	26.450700886415174	23.538837353123068
55-59	23.448996770425342	26.52230467944754	26.40297876726448	23.62571978286264
60-64	23.40074898646327	26.549340342197485	26.48235758950045	23.567553081838795
65-69	23.418683432969146	26.56871435442864	26.43572802858517	23.57687418401704
70-74	23.377063148491718	26.58342609771181	26.429440665154953	23.610070088641518
75-79	23.362437298151583	26.566951831237546	26.48022400879544	23.590386861815432
80-84	23.369707276850136	26.623290730433585	26.438906067477497	23.568095925238783
85-89	23.34509723081152	26.600048100048102	26.408688930117503	23.64616573902288
90-94	22.76400304268089	26.49630031417075	27.46109871731992	23.278597925828436
95-99	23.219610880912324	26.5540171421635	26.661963110187358	23.56440886673682
100-104	23.290447623531467	26.650107502440985	26.537989236577666	23.52145563744989
105-109	23.241166154122805	26.629443362554134	26.620135239925304	23.50925524339776
110-114	23.258291144253946	26.486594070997988	26.667274789252403	23.58783999549566
115-119	23.2814571907601	26.602865045581275	26.542553353262356	23.573124410396268
120-124	23.239842097945466	26.585645969797483	26.57243840727323	23.602073524983815
125-129	23.224243637491025	26.572049388056534	26.580705142757306	23.62300183169513
130-134	23.386992816118198	26.588793005218076	26.506945984758936	23.51726819390479
135-139	23.451491556995	26.52441529505819	26.454794843248187	23.56929830469862
140-144	23.398542008871622	26.607038398096293	26.545139083903113	23.449280509128968
145-149	23.390417675796634	26.632655314906884	26.519568677055833	23.457358332240645
150-154	23.35243279443375	26.69834419230063	26.542297022559463	23.40692599070615
155-159	23.279590204998748	26.696879270902407	26.628952106058033	23.39457841804081
160-164	23.258289431893484	26.80964360780662	26.635953933384133	23.296113026915766
165-169	23.196451751390505	26.817039507351865	26.651383179016165	23.335125562241473
170-174	23.161382396341526	26.882070843089455	26.697788839217413	23.258757921351606
175-178	23.135800616334006	26.955835701496955	26.668735594458003	23.239628087711036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	4.5
12	13.0
13	26.5
14	53.0
15	101.0
16	199.5
17	378.5
18	726.5
19	1284.0
20	2182.0
21	3547.5
22	5534.5
23	8363.5
24	11882.5
25	16175.5
26	21723.0
27	28487.5
28	35915.0
29	44360.0
30	54029.0
31	64305.0
32	75238.5
33	86814.5
34	99557.0
35	113609.5
36	128160.5
37	143277.5
38	158691.5
39	173970.0
40	187954.0
41	200997.0
42	212618.5
43	221511.0
44	227264.0
45	230822.5
46	231510.0
47	229259.5
48	225133.0
49	218803.0
50	210535.5
51	201077.5
52	191641.0
53	181575.0
54	171064.5
55	160145.0
56	149591.5
57	139309.5
58	128794.5
59	118613.5
60	108554.0
61	98089.0
62	87654.0
63	77203.5
64	66772.0
65	56927.5
66	47870.0
67	39671.0
68	31862.0
69	25008.0
70	19186.5
71	14243.0
72	10409.0
73	7369.0
74	5103.5
75	3399.5
76	2116.5
77	1330.0
78	820.0
79	474.5
80	264.5
81	146.5
82	81.5
83	43.0
84	23.0
85	9.5
86	6.5
87	3.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	429.0
92-93	876.0
94-95	1293.0
96-97	1692.0
98-99	1928.0
100-101	2318.0
102-103	2528.0
104-105	2830.0
106-107	3176.0
108-109	3421.0
110-111	3542.0
112-113	3891.0
114-115	4158.0
116-117	4347.0
118-119	4476.0
120-121	4812.0
122-123	4890.0
124-125	5229.0
126-127	5371.0
128-129	5672.0
130-131	5998.0
132-133	6228.0
134-135	48657.0
136-137	44279.0
138-139	47231.0
140-141	48883.0
142-143	41620.0
144-145	40469.0
146-147	39273.0
148-149	39573.0
150-151	39946.0
152-153	39241.0
154-155	38870.0
156-157	39027.0
158-159	41476.0
160-161	40651.0
162-163	40941.0
164-165	43403.0
166-167	43826.0
168-169	48704.0
170-171	49444.0
172-173	47235.0
174-175	46515.0
176-177	87520.0
178-179	4745311.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.69641215489159
#Duplication Level	Percentage of total
1	93.65225499020498
2	5.663209734346224
3	0.5419390664978935
4	0.09742676662467081
5	0.022756103269843907
6	0.008345931792456962
7	0.006312985957215473
8	0.002122273734861568
9	0.0010701590753059449
>10	0.004561988496537259
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.748574177145606E-4	0.0	0.0	1.7178588607160036E-5	0.0	0.0
2	0.001013536727822442	0.0	0.0	1.7178588607160036E-5	0.0	0.0
3	0.001013536727822442	1.7178588607160036E-5	0.0	1.7178588607160036E-5	0.0	0.0
4	0.001013536727822442	1.7178588607160036E-5	0.0	1.7178588607160036E-5	0.0	0.0
5	0.0010307153164296021	1.7178588607160036E-5	1.7178588607160036E-5	5.1535765821480104E-5	0.0	0.0
6	0.0010307153164296021	3.435717721432007E-5	1.7178588607160036E-5	5.1535765821480104E-5	0.0	0.0
7	0.0010307153164296021	3.435717721432007E-5	1.7178588607160036E-5	6.871435442864014E-5	0.0	0.0
8	0.0010307153164296021	3.435717721432007E-5	1.7178588607160036E-5	6.871435442864014E-5	0.0	0.0
9	0.0010307153164296021	3.435717721432007E-5	3.435717721432007E-5	6.871435442864014E-5	0.0	0.0
10-14	0.001041022469593898	5.1535765821480104E-5	7.215007215007215E-5	8.589294303580018E-5	0.0	0.0
15-19	0.0010478939050367622	6.871435442864014E-5	1.2025012025012025E-4	1.2025012025012025E-4	0.0	0.0
20-24	0.0010478939050367622	9.96358139215282E-5	1.7522160379303237E-4	1.9240019240019242E-4	1.374287088572803E-5	0.0
25-29	0.0010478939050367622	1.3399299113584827E-4	2.0614306328592042E-4	2.0614306328592042E-4	1.7178588607160036E-5	0.0
30-34	0.0011234796949082663	1.7865732151446436E-4	2.0614306328592042E-4	2.3706452277880852E-4	2.0614306328592046E-5	0.0
35-39	0.0015048443619872193	2.508073936645365E-4	2.2332165189308046E-4	2.782931354359926E-4	3.435717721432007E-5	2.748574177145606E-5
40-44	0.0016938088366659793	2.782931354359926E-4	2.2675736961451248E-4	3.023431594860166E-4	4.46643303786161E-5	5.1535765821480104E-5
45-49	0.001903387617673332	3.023431594860166E-4	2.405002405002405E-4	3.0921459492888067E-4	1.0650724936439221E-4	8.245722531436817E-5
50-54	0.0022160379303236446	3.401360544217687E-4	2.405002405002405E-4	3.2639318353604066E-4	1.443001443001443E-4	1.2025012025012025E-4
55-59	0.002394695251838109	3.7105751391465676E-4	2.405002405002405E-4	3.985432556861128E-4	1.5460729746444033E-4	1.4086442657871228E-4
60-64	0.0025149453720882294	4.225932797361369E-4	2.886002886002886E-4	4.1228612657184083E-4	1.5460729746444033E-4	1.6835016835016836E-4
65-69	0.0026626812341098054	4.466433037861609E-4	2.920360063217206E-4	4.363361506218649E-4	1.5804301518587233E-4	1.9583591012162438E-4
70-74	0.004456125884697313	4.77564763279049E-4	3.0921459492888067E-4	4.77564763279049E-4	1.7178588607160035E-4	2.1301449872878443E-4
75-79	0.03921871779014636	4.81000481000481E-4	3.0921459492888067E-4	5.565862708719852E-4	2.1301449872878443E-4	2.3019308733594448E-4
80-84	0.054198447055589904	4.81000481000481E-4	3.0921459492888067E-4	6.081220366934653E-4	3.4013605442176874E-4	2.5767882910740053E-4
85-89	0.05429121143406858	5.08486222771937E-4	3.3670033670033666E-4	6.321720607434893E-4	5.634577063148491E-4	2.5767882910740053E-4
90-94	0.054466433037861604	5.428433999862571E-4	3.470074898646327E-4	6.390434961863532E-4	5.840720126434412E-4	2.5767882910740053E-4
95-99	0.05454889026317598	6.459149316292173E-4	3.779289493575208E-4	6.734006734006733E-4	5.840720126434412E-4	2.5767882910740053E-4
100-104	0.05463134748849034	7.043221328935615E-4	3.951075379646808E-4	7.111935683364255E-4	5.978148835291692E-4	2.5767882910740053E-4
105-109	0.05467257610114753	7.249364392221535E-4	4.3977186834329694E-4	7.592936164364736E-4	6.149934721363293E-4	2.5767882910740053E-4
110-114	0.054734419020133304	7.696007696007697E-4	4.91307634164777E-4	7.902150759293618E-4	6.184291898577613E-4	2.5767882910740053E-4
115-119	0.054772211915069056	7.902150759293618E-4	5.153576582148011E-4	8.108293822579537E-4	6.184291898577613E-4	2.5767882910740053E-4
120-124	0.055146705146705145	7.902150759293618E-4	5.325362468219611E-4	8.761080189651618E-4	6.184291898577613E-4	2.645502645502645E-4
125-129	0.05564831993403422	8.108293822579537E-4	5.394076822648251E-4	9.2420806706521E-4	6.493506493506494E-4	2.748574177145606E-4
130-134	0.05600219885934171	8.417508417508417E-4	5.634577063148491E-4	9.585652442795301E-4	6.837078265649694E-4	2.920360063217206E-4
135-139	0.05653817082388511	8.486222771937058E-4	5.668934240362812E-4	9.79179550608122E-4	7.386793101078815E-4	2.989074417645846E-4
140-144	0.05703978561121418	8.864151721294578E-4	5.840720126434412E-4	0.0010272795987081701	7.558578987150416E-4	3.0921459492888067E-4
145-149	0.057256235827664405	9.31079502508074E-4	6.253006253006254E-4	0.0010478939050367622	7.558578987150416E-4	3.0921459492888067E-4
150-154	0.05740053597196454	9.894867037724179E-4	6.802721088435374E-4	0.0010650724936439222	7.558578987150416E-4	3.1608603037174465E-4
155-159	0.0576204219061362	0.001037586751872466	7.215007215007215E-4	0.0011131725417439704	7.936507936507937E-4	3.2639318353604066E-4
160-164	0.05788840788840789	0.0010788153645296503	7.249364392221535E-4	0.0011715797430083144	8.073936645365216E-4	3.2639318353604066E-4
165-167	0.05818960580865343	0.0011108820632630158	7.386793101078815E-4	0.0011853226138940425	8.073936645365217E-4	3.435717721432007E-4
>>END_MODULE
