##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293840
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8734638
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.45988671768652	39.0	39.0	39.0	38.0	39.0
2	40.28378462851008	41.0	40.0	41.0	39.0	41.0
3	40.31248553174156	41.0	40.0	41.0	39.0	41.0
4	40.323308647708124	41.0	40.0	41.0	39.0	41.0
5	40.31324698287439	41.0	40.0	41.0	39.0	41.0
6	40.30897193449803	41.0	40.0	41.0	39.0	41.0
7	40.317609384613306	41.0	40.0	41.0	39.0	41.0
8	40.30247996539754	41.0	40.0	41.0	39.0	41.0
9	40.30492643198264	41.0	40.0	41.0	39.0	41.0
10-14	40.245468146476135	41.0	40.0	41.0	38.6	41.0
15-19	40.15755391351078	41.0	40.0	41.0	38.0	41.0
20-24	40.02012784044399	41.0	40.0	41.0	38.0	41.0
25-29	39.8188470775778	41.0	40.0	41.0	37.4	41.0
30-34	39.50319106527369	40.4	39.0	41.0	36.0	41.0
35-39	39.04268992029206	40.0	37.8	41.0	35.0	41.0
40-44	38.551475470420186	39.8	36.6	41.0	35.0	41.0
45-49	38.026481784362446	39.0	35.2	41.0	35.0	41.0
50-54	37.452735900446015	37.2	35.0	40.4	35.0	41.0
55-59	36.75004848512326	35.8	35.0	39.0	35.0	41.0
60-64	35.95841918119561	35.0	34.8	37.0	33.6	40.0
65-69	35.56638970040888	35.0	35.0	36.0	33.8	39.0
70-74	35.349481867479795	35.0	35.0	35.8	34.0	37.2
75-79	35.164805937006214	35.0	35.0	35.0	34.0	36.6
80-84	35.08698249429455	35.0	35.0	35.0	34.0	36.0
85-89	34.96337073156323	35.0	34.8	35.0	33.8	36.0
90-94	39.74993958166727	40.6	39.8	40.6	37.8	40.6
95-99	40.06970257140589	41.0	40.0	41.0	38.0	41.0
100-104	39.97043741667124	41.0	40.0	41.0	38.0	41.0
105-109	39.85000276624592	41.0	40.0	41.0	37.2	41.0
110-114	39.677251919171226	41.0	39.8	41.0	36.6	41.0
115-119	39.43401391300066	40.6	39.0	41.0	35.4	41.0
120-124	39.073319474209846	40.0	38.0	41.0	35.0	41.0
125-129	38.18815236402425	39.4	36.4	40.4	34.4	40.6
130-134	38.129480684755904	39.0	35.6	40.8	34.8	41.0
135-139	37.80959203580702	38.4	35.0	41.0	35.0	41.0
140-144	37.164639810368925	36.6	35.0	40.0	34.8	41.0
145-149	36.45897787736374	35.4	35.0	38.6	34.0	41.0
150-154	35.834847776512795	35.0	35.0	36.8	34.0	39.2
155-159	35.34804533804182	35.0	35.0	36.0	34.0	37.6
160-164	35.01401155584374	35.0	35.0	35.0	34.0	36.4
165-169	34.82245338079184	35.0	35.0	35.0	34.0	36.0
170-174	34.720315008405436	35.0	35.0	35.0	34.0	36.0
175-178	34.56406674334212	35.0	34.75	35.0	33.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	57.0
30	479.0
31	3291.0
32	14967.0
33	52079.0
34	144073.0
35	377273.0
36	1201263.0
37	2921109.0
38	3159585.0
39	847509.0
40	12952.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.627783773065353	26.834323299946718	27.29374703336303	23.2441458936249
2	23.6924186211266	27.231523504465784	26.94753921112701	22.12851866328061
3	21.90762799786322	26.50657073595952	27.894699242258238	23.691102023919022
4	22.475493546498434	25.636334327764924	28.269162385436008	23.61900974030063
5	23.257002751573676	26.170403398515198	26.978805532639132	23.59378831727199
6	23.153460967701236	26.25175765727212	27.010632839048395	23.58414853597825
7	23.285670224684754	26.37046893071012	26.64982796081532	23.694032883789802
8	23.441246219934932	26.582051826303505	26.5170806162774	23.459621337484162
9	23.40570954400171	26.62483551121409	26.568840059542246	23.400614885241954
10-14	23.407614602917718	26.576055012239774	26.439334978736383	23.57699540610613
15-19	23.36161613108637	26.573888923616522	26.53674485422292	23.527750091074182
20-24	23.33818070079149	26.51368265061471	26.616484850316635	23.53165179827716
25-29	23.344280552897555	26.519498575670795	26.47297575469069	23.663245116740956
30-34	23.321135918855482	26.569957449867985	26.5435934494366	23.565313181839933
35-39	23.283034740535328	26.61506292533245	26.517804172308	23.584098161824222
40-44	23.34470644347253	26.57934993986013	26.47722092203478	23.598722694632563
45-49	23.473781054234873	26.49974274835431	26.402841193876608	23.623635003534204
50-54	23.43453271904342	26.601942747942157	26.438107681165494	23.525416851848927
55-59	23.44447016579279	26.534303997486784	26.405316396626855	23.615909440093567
60-64	23.407573387700783	26.560842017723	26.45882748661135	23.572757107964865
65-69	23.4063987540182	26.578521056052924	26.448711440588607	23.56636874934027
70-74	23.370740722168453	26.57192204187512	26.44199336022855	23.61534387572788
75-79	23.36301286899354	26.571901434266653	26.469678537336062	23.595407159403745
80-84	23.356945073167314	26.619901133853514	26.447049093505647	23.57610469947352
85-89	23.347955576407404	26.600559748440634	26.400917817086412	23.650566858065556
90-94	22.76742315525015	26.50353262395373	27.458020750484625	23.271023470311494
95-99	23.219235078030408	26.56329012615623	26.66312713061259	23.554347665200766
100-104	23.28991753961751	26.65598922928986	26.53634856641871	23.517744664673916
105-109	23.243943967656293	26.62621643724047	26.616213139023266	23.513626456079972
110-114	23.2731606001191	26.50230880812434	26.650718459916583	23.573812131839972
115-119	23.2785456302031	26.60475291196429	26.535736640256886	23.580964817575726
120-124	23.24935737357355	26.588965344505272	26.57344182724405	23.588235454677136
125-129	23.230264884328452	26.59065982446538	26.57863407641768	23.600441214788493
130-134	23.39173223672541	26.59784390469642	26.506345331866516	23.504078526711663
135-139	23.45326400505065	26.53502315706953	26.447110725439504	23.564602112440312
140-144	23.412870039391457	26.61456230897959	26.514815339523036	23.45775231210592
145-149	23.385980844923466	26.64265204109319	26.519856198242582	23.451510915740766
150-154	23.328551184603608	26.70539968886	26.560130721684832	23.40591840485156
155-159	23.284585242676002	26.713076092150857	26.60795816550532	23.39438049966782
160-164	23.23395617402564	26.83754571142723	26.629888995212198	23.298609119334937
165-169	23.205027123021804	26.819812441093433	26.64310784294911	23.332052592935653
170-174	23.160760186086847	26.90659662361225	26.693090891156118	23.239552299144787
175-178	23.129227272453363	26.990650175739795	26.663235941547804	23.216886610259042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	1.5
10	2.0
11	8.5
12	17.0
13	37.5
14	78.5
15	143.0
16	273.5
17	584.5
18	1097.0
19	1963.0
20	3336.5
21	5414.5
22	8365.0
23	12334.5
24	17814.5
25	24549.5
26	32604.0
27	42262.0
28	53602.0
29	66503.5
30	80681.5
31	95894.5
32	112467.5
33	130789.0
34	149776.5
35	170379.0
36	192545.0
37	215282.5
38	238031.5
39	260543.0
40	282490.0
41	301722.5
42	318570.5
43	332570.5
44	341830.5
45	346971.5
46	348433.5
47	345141.5
48	338014.5
49	328177.0
50	315676.0
51	301917.5
52	287398.0
53	271674.0
54	255772.5
55	240073.5
56	224752.0
57	209407.0
58	193588.0
59	178422.0
60	163077.0
61	147113.0
62	131072.5
63	115395.0
64	100406.0
65	85785.5
66	71815.5
67	59009.0
68	47412.0
69	37278.5
70	28797.5
71	21474.0
72	15474.5
73	10864.5
74	7402.0
75	4929.5
76	3204.0
77	1985.5
78	1158.0
79	694.5
80	394.0
81	199.5
82	96.0
83	45.0
84	19.0
85	9.0
86	5.0
87	2.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	641.0
92-93	1443.0
94-95	2201.0
96-97	2878.0
98-99	3533.0
100-101	3790.0
102-103	4492.0
104-105	5482.0
106-107	5864.0
108-109	7388.0
110-111	6927.0
112-113	9415.0
114-115	8743.0
116-117	7534.0
118-119	8962.0
120-121	8873.0
122-123	9525.0
124-125	10883.0
126-127	12486.0
128-129	9720.0
130-131	12611.0
132-133	10888.0
134-135	78798.0
136-137	70804.0
138-139	78602.0
140-141	68560.0
142-143	69256.0
144-145	69294.0
146-147	69618.0
148-149	64448.0
150-151	63251.0
152-153	66315.0
154-155	61786.0
156-157	64802.0
158-159	67106.0
160-161	67746.0
162-163	63390.0
164-165	67002.0
166-167	74535.0
168-169	69111.0
170-171	69739.0
172-173	73660.0
174-175	72740.0
176-177	140941.0
178-179	6988855.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.34697898354187
#Duplication Level	Percentage of total
1	91.17164882832809
2	7.598994450724807
3	0.9240035233871329
4	0.19521668944841433
5	0.05624214446775771
6	0.024682495998851608
7	0.015514683793602482
8	0.005201045999627394
9	0.0031383845098431748
>10	0.005357753341873655
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.0911412699644795E-4	1.1448671370238813E-5	0.0	1.1448671370238813E-5	0.0	0.0
2	0.001339494550317941	2.2897342740477626E-5	0.0	1.1448671370238813E-5	0.0	0.0
3	0.001339494550317941	5.724335685119406E-5	0.0	2.2897342740477626E-5	0.0	0.0
4	0.001339494550317941	6.869202822143288E-5	0.0	3.434601411071644E-5	0.0	0.0
5	0.001339494550317941	6.869202822143288E-5	0.0	3.434601411071644E-5	1.1448671370238813E-5	0.0
6	0.001339494550317941	1.0303804233214931E-4	1.1448671370238813E-5	4.579468548095525E-5	1.1448671370238813E-5	0.0
7	0.001339494550317941	1.1448671370238812E-4	2.2897342740477626E-5	4.579468548095525E-5	1.1448671370238813E-5	0.0
8	0.0013509432216881798	1.1448671370238812E-4	2.2897342740477626E-5	4.579468548095525E-5	2.2897342740477626E-5	0.0
9	0.0013509432216881798	1.2593538507262695E-4	2.2897342740477626E-5	4.579468548095525E-5	2.2897342740477626E-5	0.0
10-14	0.0013555226902362753	1.3051485362072245E-4	2.9766545562620914E-5	5.4953622577146304E-5	2.2897342740477626E-5	0.0
15-19	0.0013738405644286575	1.7859927337572547E-4	4.3504951206907486E-5	9.15893709619105E-5	2.2897342740477626E-5	0.0
20-24	0.0013761302987027053	2.2210422458263297E-4	6.869202822143288E-5	1.282251193466747E-4	2.2897342740477626E-5	0.0
25-29	0.001396737907169135	2.450015673231106E-4	1.0761751088024486E-4	1.534121963612001E-4	2.2897342740477626E-5	0.0
30-34	0.001568467977722717	2.9766545562620914E-4	1.3051485362072245E-4	1.9691714756810757E-4	2.7476811288573152E-5	0.0
35-39	0.002060760846642986	3.5490881247740314E-4	1.6257113345739116E-4	2.2897342740477622E-4	4.808441975500301E-5	2.9766545562620914E-5
40-44	0.002420249127668485	4.1215216932859725E-4	1.9920688184215532E-4	2.5187077014525384E-4	5.953309112524183E-5	7.556123104357616E-5
45-49	0.0026240354780587356	4.4420844916526593E-4	2.0607608466429863E-4	3.068243927224002E-4	8.929963668786274E-5	1.213559165245314E-4
50-54	0.0027911860800642224	4.7855446327598237E-4	2.0607608466429863E-4	3.594882810254987E-4	1.465429935390568E-4	1.8775821047191653E-4
55-59	0.002999551899002569	5.312183515790809E-4	2.175247560345374E-4	4.05282966506454E-4	1.763095391016777E-4	2.0607608466429863E-4
60-64	0.003191889578022581	5.586951628676539E-4	2.381323645009673E-4	4.4878791771336144E-4	2.3126316167882403E-4	2.2668369313072847E-4
65-69	0.003320114697369256	5.953309112524183E-4	2.999551899002569E-4	4.922928689202689E-4	2.770578471597793E-4	2.587399729673972E-4
70-74	0.005277837501680092	6.136487854448004E-4	3.2743200118883007E-4	5.060312745645555E-4	3.1598332981859126E-4	2.633194415154927E-4
75-79	0.04099769217682518	6.319666596371824E-4	3.3888067255906883E-4	5.403772886752719E-4	3.4574987538121214E-4	2.724783786116837E-4
80-84	0.05642134224681091	6.640229394738512E-4	3.549088124774032E-4	5.770130370600362E-4	4.258905749728838E-4	2.8621678425597033E-4
85-89	0.05657017497462402	7.006586878586153E-4	3.66357483847642E-4	5.999103798005138E-4	5.793027713340839E-4	2.8621678425597033E-4
90-94	0.05671442823388904	7.212662963250452E-4	3.869650923140719E-4	6.228077225409914E-4	5.930411769783705E-4	2.8621678425597033E-4
95-99	0.05678083052783641	7.441636390655227E-4	3.9841376368431064E-4	6.93789485036472E-4	5.953309112524183E-4	2.8621678425597033E-4
100-104	0.05686784043025023	7.579020447098094E-4	4.2360084069883606E-4	7.693507160800483E-4	5.953309112524183E-4	2.8621678425597033E-4
105-109	0.05694111192701976	7.71640450354096E-4	4.4420844916526593E-4	8.243043386571945E-4	5.953309112524183E-4	2.8621678425597033E-4
110-114	0.05701438342378928	7.89958324546478E-4	4.556571205355047E-4	8.723887584121974E-4	5.999103798005138E-4	2.9079625280406583E-4
115-119	0.057085365186284764	8.334632757533856E-4	4.8313393182407787E-4	9.136039753450571E-4	6.250974568150392E-4	3.0453465844835245E-4
120-124	0.0573601332991705	8.838374297824364E-4	5.472464914974152E-4	9.410807866336304E-4	6.411255967333734E-4	3.114038612704957E-4
125-129	0.05786616457373504	9.181834438931529E-4	5.815925056081317E-4	9.63978129374108E-4	6.846305479402809E-4	3.343012040109733E-4
130-134	0.05844317761079508	9.296321152633916E-4	6.159385197188482E-4	0.0010074830805810156	7.098176249548064E-4	3.503293439293077E-4
135-139	0.0591003313474468	9.616883951000602E-4	6.525742681036123E-4	0.001050988031787923	7.166868277769496E-4	3.6864721812168976E-4
140-144	0.059594913950641114	9.937446749367289E-4	6.525742681036123E-4	0.0010738853745284006	7.212662963250452E-4	3.961240294102629E-4
145-149	0.059803279769579464	0.0010464085632398274	6.731818765700422E-4	0.0011288389971055468	7.258457648731407E-4	4.007034979583584E-4
150-154	0.05993837409174828	0.001087623780172687	7.304252334212363E-4	0.0011448671370238813	7.32714967695284E-4	4.007034979583584E-4
155-159	0.060149029644960675	0.0011219697942834036	7.830891217243348E-4	0.0011723439483124543	7.441636390655229E-4	4.1215216932859725E-4
160-164	0.06043524642921664	0.0011494466055719768	8.174351358350513E-4	0.001195241291052932	7.762199189021915E-4	4.2360084069883606E-4
165-167	0.060849688332819285	0.001175396927344518	8.586503527679108E-4	0.0012479051793560306	7.785096531762393E-4	4.350495120690749E-4
>>END_MODULE
