##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293841
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9031055
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.4020732904406	39.0	39.0	39.0	37.0	39.0
2	40.208393482267574	41.0	40.0	41.0	38.0	41.0
3	40.253709893251674	41.0	40.0	41.0	39.0	41.0
4	40.27247425688361	41.0	40.0	41.0	39.0	41.0
5	40.26570838069306	41.0	40.0	41.0	39.0	41.0
6	40.26709050050077	41.0	40.0	41.0	39.0	41.0
7	40.287841896655486	41.0	40.0	41.0	39.0	41.0
8	40.26110105629962	41.0	40.0	41.0	39.0	41.0
9	40.26462611511058	41.0	40.0	41.0	39.0	41.0
10-14	40.196083403323314	41.0	40.0	41.0	38.0	41.0
15-19	40.11062428475964	41.0	40.0	41.0	38.0	41.0
20-24	39.97185427394695	41.0	40.0	41.0	38.0	41.0
25-29	39.7661397920841	41.0	39.8	41.0	37.2	41.0
30-34	39.440601170073705	40.2	38.8	41.0	35.6	41.0
35-39	38.97333188647395	40.0	37.8	41.0	35.0	41.0
40-44	38.48813933698776	39.8	36.6	41.0	35.0	41.0
45-49	37.97229158719552	39.0	35.2	41.0	35.0	41.0
50-54	37.39619189563124	37.2	35.0	40.4	35.0	41.0
55-59	36.69312531038732	35.8	35.0	39.0	34.6	41.0
60-64	35.91837212817329	35.0	34.8	37.0	33.6	40.0
65-69	35.539508750638774	35.0	35.0	36.0	33.8	39.0
70-74	35.338419664147764	35.0	35.0	35.8	34.0	37.2
75-79	35.17351897425051	35.0	35.0	35.0	34.0	36.8
80-84	35.11306659078037	35.0	35.0	35.0	34.0	36.0
85-89	34.9979984398279	35.0	34.8	35.0	33.8	36.0
90-94	39.73992258786183	40.6	39.8	40.6	37.8	40.6
95-99	40.065243118035156	41.0	40.0	41.0	38.0	41.0
100-104	39.95134865802622	41.0	40.0	41.0	38.0	41.0
105-109	39.831065756447316	41.0	40.0	41.0	37.2	41.0
110-114	39.65890262314295	41.0	39.6	41.0	36.4	41.0
115-119	39.40991012839654	40.6	39.0	41.0	35.2	41.0
120-124	39.06377887147208	40.0	38.0	41.0	35.0	41.0
125-129	38.17313263393927	39.4	36.4	40.4	34.4	40.6
130-134	38.1194367557333	39.0	35.6	40.8	34.8	41.0
135-139	37.805290369958584	38.4	35.0	41.0	35.0	41.0
140-144	37.16668215152931	36.6	35.0	40.0	34.8	41.0
145-149	36.46921186956159	35.4	35.0	38.6	34.0	41.0
150-154	35.845613161172174	35.0	35.0	36.8	34.0	39.2
155-159	35.3591801294896	35.0	35.0	36.0	34.0	37.6
160-164	35.022329098445894	35.0	35.0	35.0	34.0	36.4
165-169	34.829017978872244	35.0	35.0	35.0	34.0	36.0
170-174	34.72910779171929	35.0	35.0	35.0	34.0	36.0
175-178	34.57118107956352	35.0	34.75	35.0	33.75	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	30.0
30	455.0
31	2955.0
32	15408.0
33	54646.0
34	153228.0
35	403960.0
36	1266574.0
37	3030552.0
38	3226535.0
39	861209.0
40	15502.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.600393863175455	26.875830121729965	27.236873211380065	23.286902803714515
2	23.705214949969854	27.19522802153237	26.98640413550798	22.113152892989802
3	21.89726449456902	26.515805739196587	27.918454709887165	23.668475056347237
4	22.497736975358915	25.591461905613468	28.295066301777588	23.615734817250033
5	23.27850954290501	26.139271657630253	26.97611740820978	23.606101391254953
6	23.139201344693394	26.279487833924165	26.999780202866663	23.581530618515778
7	23.314430041672875	26.381070650106768	26.5920869710128	23.71241233720756
8	23.470502615696613	26.600978512477226	26.468037233745118	23.460481638081042
9	23.39120955414401	26.62736524138099	26.595586008500664	23.385839195974334
10-14	23.413962156137906	26.582628496892113	26.426228164926467	23.577181182043518
15-19	23.358936469770143	26.573486707809884	26.528289330537795	23.539287491882178
20-24	23.3523835255128	26.50984187340239	26.603345899233254	23.534428701851557
25-29	23.347081819344474	26.524586551626584	26.462651373510624	23.66568025551832
30-34	23.31318544732592	26.566701232580247	26.535920775590448	23.58419254450338
35-39	23.280347644876485	26.602298402567587	26.527151036063895	23.590202916492036
40-44	23.335862753576407	26.572377202884933	26.487009546503703	23.604750497034953
45-49	23.485763291221236	26.485931045708394	26.401559950637	23.626745712433376
50-54	23.43335523922731	26.57327410806379	26.45487376613253	23.538496886576375
55-59	23.44963240728796	26.517156633416583	26.399719634084835	23.63349132521062
60-64	23.403194864830297	26.564364849953854	26.466376298228724	23.566063986987125
65-69	23.40643479637761	26.574416831699065	26.453795265337217	23.565353106586105
70-74	23.37169466911673	26.572255400947064	26.443413311069413	23.612636618866787
75-79	23.354467445940703	26.57249014650005	26.48697854237406	23.586063865185185
80-84	23.351572988980802	26.619456973742274	26.44659123435745	23.582378802919482
85-89	23.34253749977162	26.59917584379677	26.415622538009124	23.642664118422488
90-94	22.781958437603738	26.488980035453192	27.444857652571297	23.284203874371777
95-99	23.229447875927818	26.543532606390425	26.663884574511204	23.56313494317055
100-104	23.306082821182724	26.643740350865986	26.52150171805388	23.528675109897414
105-109	23.24423855019324	26.614139046158247	26.617955870578104	23.523666533070404
110-114	23.264640230750487	26.512222226624516	26.64993504221666	23.57320250040834
115-119	23.294564851776645	26.5830935527301	26.540533067346473	23.581808528146777
120-124	23.252327626111864	26.57941643129344	26.56568677680354	23.60256916579116
125-129	23.236539174707442	26.586079144082486	26.56960200231375	23.607779678896314
130-134	23.378925718715657	26.580488279232057	26.521046760353954	23.519539241698332
135-139	23.45236421270301	26.52236630076359	26.453019471113475	23.57225001541993
140-144	23.406793911856337	26.614265258810715	26.526166461088767	23.452774368244178
145-149	23.396847100804905	26.63430236398043	26.526883732594637	23.44196680262003
150-154	23.34149693776077	26.680905331284617	26.554424757255703	23.42317297369891
155-159	23.302814839641083	26.684481354614896	26.614047691479474	23.39865611426454
160-164	23.252008154610195	26.805095652249445	26.632662483208787	23.310233709931573
165-169	23.2052014455407	26.811831723380703	26.657257893742386	23.32570893733621
170-174	23.170671240977455	26.882217554870603	26.707295843796132	23.239815360355813
175-178	23.145293959070944	26.94829616317239	26.66748923031897	23.23892064743769
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	1.0
10	4.5
11	12.0
12	24.0
13	41.5
14	80.5
15	151.0
16	307.5
17	615.5
18	1145.5
19	2009.5
20	3414.0
21	5567.5
22	8591.5
23	12809.0
24	18290.5
25	25276.0
26	33676.0
27	43806.5
28	55738.5
29	68704.5
30	83446.0
31	99541.5
32	116576.5
33	134868.5
34	154877.5
35	176106.0
36	198633.5
37	222208.5
38	246442.0
39	269705.0
40	291192.5
41	311764.0
42	329204.0
43	343886.0
44	353971.0
45	358778.5
46	359882.0
47	357267.5
48	350278.5
49	339132.0
50	326065.5
51	311343.0
52	296012.0
53	280322.5
54	264579.5
55	248566.5
56	232055.5
57	216295.0
58	200293.0
59	183966.5
60	168254.0
61	152172.0
62	135743.5
63	119822.0
64	104085.5
65	89023.5
66	74620.0
67	61367.0
68	49408.5
69	38572.0
70	29617.5
71	22176.0
72	16222.0
73	11517.0
74	7812.5
75	5205.5
76	3339.5
77	2108.5
78	1294.5
79	743.5
80	427.5
81	236.0
82	119.5
83	57.0
84	27.5
85	10.5
86	5.0
87	4.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	696.0
92-93	1897.0
94-95	2093.0
96-97	2857.0
98-99	3514.0
100-101	4802.0
102-103	4707.0
104-105	5182.0
106-107	5738.0
108-109	6614.0
110-111	7559.0
112-113	8185.0
114-115	7386.0
116-117	10524.0
118-119	9901.0
120-121	9595.0
122-123	10434.0
124-125	9606.0
126-127	10864.0
128-129	10320.0
130-131	11484.0
132-133	11882.0
134-135	83337.0
136-137	80548.0
138-139	82296.0
140-141	76614.0
142-143	85304.0
144-145	72780.0
146-147	72359.0
148-149	75706.0
150-151	74235.0
152-153	83240.0
154-155	82509.0
156-157	76107.0
158-159	70294.0
160-161	77056.0
162-163	77072.0
164-165	85646.0
166-167	88081.0
168-169	77610.0
170-171	78578.0
172-173	84106.0
174-175	75340.0
176-177	146298.0
178-179	7070099.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.16959584290821
#Duplication Level	Percentage of total
1	90.86686410092463
2	7.786414731381362
3	1.0075223971438192
4	0.20323414383872632
5	0.06641751979480315
6	0.028769044919035716
7	0.014464021540364191
8	0.008310748086684335
9	0.005222816157034181
>10	0.012780476213550105
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2180193786883149E-4	0.0	1.1072903442621044E-5	1.1072903442621044E-5	0.0	0.0
2	0.001218019378688315	3.321871032786313E-5	3.321871032786313E-5	2.214580688524209E-5	0.0	0.0
3	0.001218019378688315	6.643742065572626E-5	3.321871032786313E-5	3.321871032786313E-5	0.0	0.0
4	0.001218019378688315	7.751032409834732E-5	3.321871032786313E-5	3.321871032786313E-5	0.0	0.0
5	0.001218019378688315	7.751032409834732E-5	3.321871032786313E-5	4.429161377048418E-5	2.214580688524209E-5	0.0
6	0.001218019378688315	7.751032409834732E-5	4.429161377048418E-5	4.429161377048418E-5	2.214580688524209E-5	0.0
7	0.001218019378688315	7.751032409834732E-5	5.5364517213105224E-5	6.643742065572626E-5	2.214580688524209E-5	0.0
8	0.001218019378688315	7.751032409834732E-5	6.643742065572626E-5	6.643742065572626E-5	2.214580688524209E-5	0.0
9	0.001229092282130936	9.965613098358941E-5	6.643742065572626E-5	6.643742065572626E-5	2.214580688524209E-5	0.0
10-14	0.001229092282130936	9.965613098358941E-5	7.751032409834732E-5	6.643742065572626E-5	2.214580688524209E-5	0.0
15-19	0.0012534526697047022	1.262310992458799E-4	1.240165185573557E-4	9.079780822949257E-5	2.214580688524209E-5	0.0
20-24	0.0012866713800325654	1.9709768127865456E-4	1.6166439026226727E-4	1.5059148681964622E-4	3.321871032786313E-5	0.0
25-29	0.0012955297027866623	2.3696013367209036E-4	2.037414233442272E-4	1.8602477783603359E-4	3.321871032786313E-5	0.0
30-34	0.0014151170599669698	2.5689135986880823E-4	2.236726495409451E-4	2.458184564261872E-4	5.09353558360568E-5	0.0
35-39	0.0018203853259668996	2.9232465088519557E-4	2.5024761780323564E-4	3.0561213501634085E-4	8.193948547539574E-5	1.7716645508193672E-5
40-44	0.0021990786237045395	3.144704577704377E-4	3.188996191474861E-4	3.476891680983008E-4	1.240165185573557E-4	5.5364517213105224E-5
45-49	0.0025290511462946466	3.2997252259010714E-4	3.676203942950187E-4	3.543329101638734E-4	1.683081323278399E-4	1.1294361511473465E-4
50-54	0.002754938376524116	3.676203942950187E-4	3.875516204917366E-4	3.543329101638734E-4	1.9709768127865456E-4	1.3730400268850094E-4
55-59	0.0029210319281634317	3.7869329773763976E-4	3.875516204917366E-4	4.03053685311406E-4	2.3031639160651772E-4	1.6609355163931569E-4
60-64	0.003033975543278166	4.274140728851723E-4	4.2298491150812386E-4	4.3184323426222074E-4	2.5467677918028405E-4	1.8823935852455776E-4
65-69	0.0032820085803928777	4.4070155701631756E-4	4.6063278321303543E-4	4.87207751475326E-4	2.7017884399995346E-4	2.081705847212756E-4
70-74	0.005073604357408963	4.672765252786081E-4	4.739202673441807E-4	5.381431073113827E-4	2.856809088196229E-4	2.5467677918028405E-4
75-79	0.03923572605858341	5.159973004261407E-4	5.049243969835196E-4	6.001513665900607E-4	2.989683929507682E-4	2.5467677918028405E-4
80-84	0.053851958602843183	5.470014300654796E-4	5.270702038687616E-4	6.377992382949722E-4	3.875516204917366E-4	2.635351019343808E-4
85-89	0.05392725434625302	6.134388507212059E-4	5.580743335081007E-4	6.931637555080774E-4	6.222971734753028E-4	2.6574968262290505E-4
90-94	0.05412213744684315	6.533013031146417E-4	5.780055597048186E-4	7.020220782621743E-4	6.422283996720206E-4	2.6574968262290505E-4
95-99	0.054217364416449684	7.153095623933195E-4	6.267263348523511E-4	7.396699499670858E-4	6.444429803605449E-4	2.6574968262290505E-4
100-104	0.05425058312677755	7.596011761638038E-4	6.621596258687385E-4	7.795324023605216E-4	6.533013031146417E-4	2.856809088196229E-4
105-109	0.05429044557917099	8.061073706228121E-4	6.865200134425047E-4	8.149656933769088E-4	6.533013031146417E-4	2.8789548950814716E-4
110-114	0.054305947643990646	8.371115002621509E-4	7.285970465244647E-4	8.415406616391994E-4	6.533013031146417E-4	2.8789548950814716E-4
115-119	0.054365741322580804	9.101926629834498E-4	7.485282727211827E-4	8.548281457703445E-4	6.533013031146417E-4	2.989683929507682E-4
120-124	0.05464699307002339	9.54484276753934E-4	7.529574340982311E-4	8.836176947211594E-4	6.577304644916902E-4	3.1004129639338926E-4
125-129	0.05517849243526919	9.85488406393273E-4	7.551720147867553E-4	9.190509857375467E-4	6.798762713769322E-4	3.2332878052453455E-4
130-134	0.05572770844602319	0.0010142779553440878	7.662449182293763E-4	9.611280188195067E-4	7.064512396392226E-4	3.410454260327282E-4
135-139	0.05629242652159687	0.0010519258270489992	8.193948547539573E-4	9.85488406393273E-4	7.197387237703679E-4	3.543329101638734E-4
140-144	0.0567796342730722	0.0011050757635735803	8.526135650818204E-4	0.0010253508587867088	7.374553692785616E-4	3.676203942950187E-4
145-149	0.05715389840943278	0.0011272215704588224	8.769739526555867E-4	0.0010541404077375233	7.551720147867553E-4	3.7647871704911553E-4
150-154	0.05735542525208849	0.0011648694421637338	8.991197595408288E-4	0.0010740716339342413	7.994636285572395E-4	3.9862452393435763E-4
155-159	0.05758574164369501	0.0011848006683604518	9.12407243671974E-4	0.001102861182885056	8.127511126883848E-4	4.119120080655028E-4
160-164	0.05793785997317036	0.0012025173138686454	9.323384698686919E-4	0.001125006989770298	8.48184403704772E-4	4.384869763277933E-4
165-167	0.05839480178856918	0.001218019378688315	9.55960663879617E-4	0.0011626548614752097	8.710684041528556E-4	4.798258158469119E-4
>>END_MODULE
