##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293850
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7363809
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.39025048042392	39.0	39.0	39.0	37.0	39.0
2	40.1923387746749	41.0	40.0	41.0	38.0	41.0
3	40.2271186012565	41.0	40.0	41.0	38.0	41.0
4	40.232356243894976	41.0	40.0	41.0	38.0	41.0
5	40.23083678569066	41.0	40.0	41.0	38.0	41.0
6	40.21548766406082	41.0	40.0	41.0	38.0	41.0
7	40.22634087874903	41.0	40.0	41.0	38.0	41.0
8	40.20542982578717	41.0	40.0	41.0	38.0	41.0
9	40.18596571421122	41.0	40.0	41.0	38.0	41.0
10-14	40.11719070931905	41.0	40.0	41.0	38.0	41.0
15-19	39.95964018078144	41.0	40.0	41.0	38.0	41.0
20-24	39.71319114876553	41.0	39.6	41.0	37.2	41.0
25-29	39.32195957282433	40.0	38.6	41.0	35.4	41.0
30-34	38.86529267665688	40.0	37.6	41.0	35.0	41.0
35-39	38.39674399485375	39.4	36.0	41.0	35.0	41.0
40-44	37.567422023031824	37.6	35.0	40.6	35.0	41.0
45-49	36.64737336343189	35.8	35.0	39.2	34.0	41.0
50-54	35.90551210657419	35.0	35.0	37.2	34.0	39.8
55-59	35.40128656242985	35.0	35.0	35.8	33.2	38.8
60-64	34.980432001970726	35.0	34.6	35.0	32.6	37.0
65-69	34.97036566266182	35.0	34.8	35.0	33.0	36.8
70-74	35.03336569430304	35.0	35.0	35.0	33.0	36.0
75-79	35.028314992960844	35.0	35.0	35.0	33.0	36.2
80-84	35.02979553108995	35.0	35.0	35.0	33.0	37.0
85-89	34.86059396706243	35.0	34.6	35.0	32.4	38.2
90-94	39.5253655979826	40.6	39.8	40.6	37.0	40.6
95-99	39.763052450365635	41.0	40.0	41.0	36.8	41.0
100-104	39.600838806771506	41.0	39.4	41.0	35.8	41.0
105-109	39.395588573741705	41.0	39.0	41.0	35.0	41.0
110-114	39.12248446638464	40.0	38.2	41.0	35.0	41.0
115-119	38.69329886368551	40.0	37.4	41.0	35.0	41.0
120-124	38.2034143548274	39.4	35.6	41.0	34.0	41.0
125-129	37.411329335109905	38.2	35.0	40.4	33.6	40.8
130-134	36.81046070502894	36.6	35.0	39.6	33.0	41.0
135-139	36.242933239534935	35.0	35.0	38.4	33.8	41.0
140-144	35.64526650371709	35.0	35.0	36.6	33.2	39.4
145-149	35.1533271154727	35.0	35.0	35.4	33.0	37.4
150-154	34.83256645518274	35.0	35.0	35.0	33.0	36.2
155-159	34.64906437000057	35.0	35.0	35.0	33.0	35.8
160-164	34.534920581314765	35.0	35.0	35.0	33.0	35.0
165-169	34.44995908365366	35.0	35.0	35.0	33.0	35.0
170-174	34.39041901209138	35.0	34.6	35.0	33.0	35.0
175-178	34.07215940536031	34.75	33.75	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	2.0
29	43.0
30	524.0
31	3705.0
32	19737.0
33	73724.0
34	217836.0
35	594648.0
36	1890603.0
37	3213318.0
38	1230535.0
39	117380.0
40	1753.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.104832431150783	22.601156548194012	23.984801887175507	27.3092091334797
2	27.604015802148048	22.959761721141874	23.537329661863854	25.89889281484623
3	25.518356057306757	22.08229463854915	24.26945076929616	28.129898534847932
4	26.326484024775766	21.616652468851377	24.494008467628642	27.562855038744217
5	27.290102174024337	22.08828338703516	22.945095398318998	27.676519040621507
6	27.28202211654322	22.2503326742994	22.77101429436858	27.6966309147888
7	27.288554062170817	22.47171266935359	22.652638057288016	27.587095211187577
8	27.36105730064427	22.490086312667806	22.538430858269138	27.610425528418787
9	27.602657809294072	22.54473194511156	22.344237880151425	27.50837236544294
10-14	27.44137714598518	22.551464873681542	22.43337381510031	27.57378416523297
15-19	27.426352313048856	22.519954007498022	22.4972673788796	27.556426300573523
20-24	27.330160247230744	22.453933826909417	22.593473024626253	27.622432901233584
25-29	27.29728052425043	22.482736855342118	22.540858949492034	27.679123670915416
30-34	27.276166451356897	22.596672455790205	22.585792217044194	27.541368875808704
35-39	27.28114213717385	22.579708409058412	22.63762680428023	27.501522649487516
40-44	27.23577159592271	22.676370340403995	22.65233115090302	27.435526912770282
45-49	27.34092369859131	22.63765396413731	22.60061063506672	27.420811702204663
50-54	27.279099715921472	22.714758082400017	22.641708930799265	27.36443327087924
55-59	27.189132689345964	22.725885475845452	22.68861128798968	27.39637054681891
60-64	27.179659331196664	22.78764155887259	22.726195098216152	27.306504011714587
65-69	27.138550171521285	22.765088013553854	22.749851333732312	27.34651048119255
70-74	27.060471014389424	22.830361298072777	22.78149799920123	27.327669688336563
75-79	27.049707563028864	22.828870221919118	22.799763003087126	27.321659211964892
80-84	27.038001664627643	22.846852763291388	22.776074175742473	27.3390713963385
85-89	27.006887332357483	22.839568489622692	22.748425441235643	27.405118736784186
90-94	26.616373622597344	22.40128752945574	23.682522771024395	27.29981607692252
95-99	27.284744624403466	22.607915737697525	22.57785492772229	27.52948471017672
100-104	27.352463523899097	22.64613422788684	22.501932963050773	27.499469285163297
105-109	27.271694856267075	22.65422764723299	22.582134955222973	27.491942541276966
110-114	27.267560108370976	22.58364367893536	22.620409618305796	27.528386594387865
115-119	27.241282013968686	22.660594029332998	22.542824311100127	27.555299645598186
120-124	27.131650560130556	22.740556313240713	22.63179148272601	27.496001643902723
125-129	27.11154370315895	22.769221996680127	22.655483214042256	27.46375108611867
130-134	27.20815422028672	22.78776498096644	22.58446163974675	27.419619159000092
135-139	27.308689624124888	22.759063383073592	22.56960494000809	27.362642052793433
140-144	27.27670515820383	22.861241734978393	22.63441926065057	27.227633846167205
145-149	27.22963275606095	22.878932674060792	22.639591580120083	27.251842989758174
150-154	27.123491803145722	23.016707924546523	22.698861203323357	27.160939068984398
155-159	27.089308426733528	23.017586180056064	22.753847703400144	27.13925768981027
160-164	27.04025025237004	23.113594635940295	22.76374842552788	27.082406686161786
165-169	26.933590532327656	23.155649251420428	22.839822248626255	27.070937967625657
170-174	26.930124047704457	23.244784794980205	22.858036409725372	26.967054747589962
175-178	26.91859553849465	23.25128883793727	22.791189380620345	27.03892624294773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	2.5
13	4.5
14	6.5
15	18.5
16	37.0
17	58.5
18	107.5
19	205.0
20	367.0
21	577.5
22	909.0
23	1398.0
24	2022.0
25	2745.5
26	3696.5
27	4887.5
28	6165.5
29	7685.5
30	9575.5
31	11487.0
32	13729.0
33	16603.5
34	19975.5
35	23909.5
36	28432.5
37	34025.0
38	40417.5
39	48248.0
40	58339.5
41	70583.0
42	85262.5
43	102891.5
44	123354.5
45	147118.0
46	174562.0
47	204128.5
48	234491.5
49	264403.0
50	291807.5
51	314370.0
52	332156.5
53	345363.0
54	351677.0
55	352787.0
56	350030.0
57	343465.5
58	334821.0
59	323722.5
60	309654.5
61	292602.5
62	272847.0
63	249778.0
64	223685.0
65	195885.0
66	167779.5
67	139996.5
68	113321.0
69	89095.0
70	68111.0
71	50879.0
72	36699.0
73	25481.5
74	17288.0
75	11387.0
76	7214.5
77	4428.5
78	2573.5
79	1447.5
80	855.5
81	467.5
82	246.5
83	125.5
84	54.0
85	24.5
86	12.0
87	3.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1153.0
92-93	2458.0
94-95	3753.0
96-97	5088.0
98-99	5911.0
100-101	6970.0
102-103	7975.0
104-105	9422.0
106-107	10709.0
108-109	12392.0
110-111	13627.0
112-113	13227.0
114-115	14957.0
116-117	15711.0
118-119	15769.0
120-121	16289.0
122-123	17028.0
124-125	19984.0
126-127	18475.0
128-129	18960.0
130-131	19453.0
132-133	22374.0
134-135	98298.0
136-137	96709.0
138-139	87593.0
140-141	85701.0
142-143	83838.0
144-145	88630.0
146-147	91772.0
148-149	96919.0
150-151	96792.0
152-153	103844.0
154-155	86988.0
156-157	86937.0
158-159	110426.0
160-161	91079.0
162-163	92736.0
164-165	96434.0
166-167	98072.0
168-169	103525.0
170-171	100711.0
172-173	108043.0
174-175	111385.0
176-177	296267.0
178-179	4779425.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.0344087355201
#Duplication Level	Percentage of total
1	88.68885323124516
2	9.715126353267339
3	1.2021772650402174
4	0.2498236963794007
5	0.08418299385877197
6	0.021705310037330512
7	0.016649303968739506
8	0.003142823358474566
9	0.003163995143652872
>10	0.015175027700886625
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.505949977790027E-5	0.0	1.3579928539700038E-5	0.0	0.0	0.0
2	6.92576355524702E-4	0.0	2.7159857079400075E-5	0.0	0.0	0.0
3	6.92576355524702E-4	1.3579928539700038E-5	5.431971415880015E-5	0.0	0.0	0.0
4	6.92576355524702E-4	2.7159857079400075E-5	8.147957123820023E-5	0.0	0.0	0.0
5	6.92576355524702E-4	2.7159857079400075E-5	8.147957123820023E-5	0.0	0.0	0.0
6	6.92576355524702E-4	2.7159857079400075E-5	8.147957123820023E-5	0.0	1.3579928539700038E-5	0.0
7	6.92576355524702E-4	2.7159857079400075E-5	9.505949977790027E-5	0.0	1.3579928539700038E-5	0.0
8	6.92576355524702E-4	2.7159857079400075E-5	9.505949977790027E-5	0.0	1.3579928539700038E-5	0.0
9	6.92576355524702E-4	4.073978561910011E-5	9.505949977790027E-5	0.0	1.3579928539700038E-5	0.0
10-14	7.034402983564621E-4	5.160372845086014E-5	1.2221935685730035E-4	5.431971415880015E-6	1.357992853970004E-5	0.0
15-19	7.496120553914421E-4	6.246767128262017E-5	1.7653907101610047E-4	1.357992853970004E-5	1.357992853970004E-5	0.0
20-24	7.957838124264222E-4	6.78996426985002E-5	2.009829423875606E-4	1.6295914247640046E-5	2.444387137146007E-5	0.0
25-29	8.310916266296424E-4	8.419555694614024E-5	2.444387137146007E-4	4.073978561910011E-5	6.518365699056018E-5	0.0
30-34	8.854113407884424E-4	1.0592344260966029E-4	2.7974652791782083E-4	5.431971415880016E-5	9.505949977790027E-5	5.431971415880015E-6
35-39	9.696068977345828E-4	1.3036731398112037E-4	3.50362156324261E-4	6.78996426985002E-5	1.0863942831760031E-4	3.259182849528009E-5
40-44	0.001056518440388663	1.6295914247640045E-4	4.046818704830612E-4	6.78996426985002E-5	1.5481118535258046E-4	6.246767128262017E-5
45-49	0.0011352820259189233	1.6839111389228048E-4	4.3727369897834126E-4	7.876358553026021E-5	2.2542681375902065E-4	7.061562840644019E-5
50-54	0.0011705898401221434	2.009829423875606E-4	4.617175703498013E-4	8.962752836202025E-5	2.715985707940008E-4	8.962752836202025E-5
55-59	0.0012493534256524034	2.0369892809550056E-4	4.752974988895013E-4	1.0863942831760031E-4	2.9875842787340087E-4	1.493792139367004E-4
60-64	0.0012900932112715037	2.0369892809550056E-4	5.459131272959416E-4	1.1678738544142032E-4	3.3678222778456095E-4	1.8468702813992052E-4
65-69	0.0013525608825541238	2.3629075659078067E-4	5.757889700832816E-4	1.2493534256524034E-4	3.856699705274811E-4	2.0913089951138055E-4
70-74	0.001765390710161005	2.6616659937812073E-4	6.708484698611819E-4	1.357992853970004E-4	4.6986552747362134E-4	2.3085878517490065E-4
75-79	0.008875841293547945	3.0147441358134087E-4	7.197362126041021E-4	1.5209519964464043E-4	5.513450987118216E-4	2.5801864225430074E-4
80-84	0.011877005500821654	3.259182849528009E-4	7.523280410993821E-4	1.765390710161005E-4	5.757889700832816E-4	2.715985707940008E-4
85-89	0.011896017400777234	3.802379991116011E-4	7.713399410549622E-4	1.8740301384786054E-4	6.056648128706218E-4	2.715985707940008E-4
90-94	0.012015520771926594	3.883859562354211E-4	7.876358553026023E-4	2.0369892809550056E-4	6.110967842865017E-4	2.715985707940008E-4
95-99	0.012080704428917153	4.4542165610216125E-4	8.310916266296422E-4	2.145628709272606E-4	6.110967842865017E-4	2.715985707940008E-4
100-104	0.012137740128783894	4.4813764181010125E-4	8.826953550805025E-4	2.1727885663520063E-4	6.110967842865017E-4	2.715985707940008E-4
105-109	0.012194775828650636	4.508536275180413E-4	9.288671121154828E-4	2.2814279946696062E-4	6.110967842865017E-4	2.715985707940008E-4
110-114	0.012238231599977676	4.617175703498013E-4	0.0010103466833536827	2.3085878517490065E-4	6.110967842865017E-4	2.715985707940008E-4
115-119	0.012278971385596775	4.752974988895014E-4	0.001080962311760123	2.3357477088284064E-4	6.165287557023818E-4	2.715985707940008E-4
120-124	0.012374030885374674	4.888774274292014E-4	0.0011787377972459633	2.4987068513048074E-4	6.545525556135418E-4	2.715985707940008E-4
125-129	0.012504398199355796	5.024573559689015E-4	0.0012384894828206434	2.6616659937812073E-4	7.088722697723421E-4	2.770305422098808E-4
130-134	0.012651061427584553	5.160372845086015E-4	0.0012792292684397437	2.715985707940008E-4	7.414640982676221E-4	3.0147441358134087E-4
135-139	0.012814020570060957	5.214692559244816E-4	0.0013634248253858838	2.770305422098808E-4	7.767719124708422E-4	3.2048631353692085E-4
140-144	0.012960683798289716	5.513450987118214E-4	0.0014693482679955443	2.851784993337008E-4	8.039317695502423E-4	3.44930184908381E-4
145-149	0.013053027312359678	5.703569986674016E-4	0.0015616917820655044	2.851784993337008E-4	8.147957123820022E-4	3.5851011344808093E-4
150-154	0.013101915055102598	5.920848843309217E-4	0.0016377393818878248	2.933264564575208E-4	8.338076123375823E-4	3.6665807057190104E-4
155-159	0.013218702440544017	6.219607271182616E-4	0.0016676152246751647	3.0147441358134087E-4	8.555354980011025E-4	3.7752201340366103E-4
160-164	0.013351785740233078	6.382566413659018E-4	0.0016839111389228049	3.259182849528009E-4	8.636834551249224E-4	3.802379991116011E-4
165-167	0.013475815754229006	6.518365699056018E-4	0.001692964424615938	3.3497157064593425E-4	8.736420693873691E-4	3.983445704978678E-4
>>END_MODULE
