##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293851
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7365731
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.364143898277035	39.0	39.0	39.0	37.0	39.0
2	40.16532792196728	41.0	40.0	41.0	38.0	41.0
3	40.20564978547275	41.0	40.0	41.0	38.0	41.0
4	40.210943082227686	41.0	40.0	41.0	38.0	41.0
5	40.20555271431987	41.0	40.0	41.0	38.0	41.0
6	40.19128637741454	41.0	40.0	41.0	38.0	41.0
7	40.20117107181894	41.0	40.0	41.0	38.0	41.0
8	40.18536639472715	41.0	40.0	41.0	38.0	41.0
9	40.16313628613372	41.0	40.0	41.0	38.0	41.0
10-14	40.087828485726675	41.0	40.0	41.0	38.0	41.0
15-19	39.93479180817219	41.0	40.0	41.0	38.0	41.0
20-24	39.68328547431341	41.0	39.4	41.0	36.8	41.0
25-29	39.291668729145826	40.0	38.6	41.0	35.4	41.0
30-34	38.833473066013404	40.0	37.6	41.0	35.0	41.0
35-39	38.377059819317324	39.4	36.0	41.0	35.0	41.0
40-44	37.55479674726106	37.6	35.0	40.6	34.8	41.0
45-49	36.63731415116843	35.8	35.0	39.2	34.0	41.0
50-54	35.90302871500466	35.0	35.0	37.2	33.8	40.0
55-59	35.41273492067522	35.0	35.0	36.0	33.0	39.0
60-64	35.00322523317781	35.0	34.6	35.0	32.8	37.2
65-69	34.98516285756295	35.0	34.8	35.0	33.0	37.0
70-74	35.029374463987345	35.0	35.0	35.0	33.0	37.0
75-79	35.02040891800148	35.0	35.0	35.0	33.0	37.0
80-84	35.02686926796539	35.0	35.0	35.0	33.0	37.6
85-89	34.85990696646402	35.0	34.6	35.0	32.4	38.2
90-94	39.51421517180548	40.6	39.8	40.6	37.0	40.6
95-99	39.72063900658905	41.0	40.0	41.0	36.6	41.0
100-104	39.55344797059111	41.0	39.2	41.0	35.6	41.0
105-109	39.344018397165726	40.8	39.0	41.0	35.0	41.0
110-114	39.06721648310205	40.0	38.0	41.0	35.0	41.0
115-119	38.64014576012666	40.0	37.4	41.0	34.8	41.0
120-124	38.148081902106995	39.4	35.6	41.0	34.0	41.0
125-129	37.359719455638306	38.0	35.0	40.4	33.6	40.8
130-134	36.756160971798764	36.4	35.0	39.6	33.0	41.0
135-139	36.20932559623778	35.0	35.0	38.4	33.0	41.0
140-144	35.62190075624433	35.0	35.0	36.6	33.0	39.4
145-149	35.123256961030776	35.0	35.0	35.4	33.0	37.6
150-154	34.79717438260643	35.0	35.0	35.0	33.0	36.2
155-159	34.602035582022985	35.0	35.0	35.0	33.0	35.8
160-164	34.48268368553731	35.0	35.0	35.0	33.0	35.0
165-169	34.39629653620498	35.0	34.4	35.0	33.0	35.0
170-174	34.32554328035816	35.0	34.0	35.0	33.0	35.0
175-178	33.999144535543266	34.75	33.75	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	62.0
30	564.0
31	3884.0
32	20299.0
33	76422.0
34	226526.0
35	623823.0
36	1930441.0
37	3170928.0
38	1198029.0
39	113293.0
40	1459.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.071845414935733	22.660805831763337	24.00761037838607	27.25973837491486
2	27.5598443657527	22.98568872526026	23.57168623182139	25.882780677165645
3	25.48522339466375	22.111138731512188	24.303032516392467	28.100605357431597
4	26.29836739897235	21.650858550224005	24.51880200349429	27.531972047309356
5	27.250275634556843	22.09359804206806	23.00787253838078	27.64825378499432
6	27.253085946255705	22.27901073226812	22.798782632708146	27.66912068876803
7	27.22955807101834	22.501229002253815	22.685311206722048	27.583901720005795
8	27.330579951942312	22.53833326250986	22.54498569116901	27.58610109437882
9	27.55034089623963	22.587181095807054	22.379489557791345	27.482988450161972
10-14	27.395591829242743	22.59723033599788	22.486653395297765	27.520524439461607
15-19	27.3911224832946	22.551149369967487	22.53368742355647	27.524040723181447
20-24	27.279633209521226	22.493900469620733	22.645130537620776	27.581335783237265
25-29	27.23340833380964	22.529345695627494	22.591332754345768	27.6459132162171
30-34	27.218990212919802	22.639651108627238	22.63226012462307	27.50909855382989
35-39	27.211878902447022	22.652524236901943	22.670434204018584	27.46516265663245
40-44	27.16900467855804	22.726045792332084	22.69239264914779	27.412556879962086
45-49	27.321271982373506	22.682261950646854	22.62946881986323	27.36699724711641
50-54	27.242770608918516	22.76834980805028	22.679720451371356	27.309159131659843
55-59	27.16879288695175	22.758357588676535	22.717237433731967	27.355612090639745
60-64	27.134488077286555	22.84134188446469	22.74286421809322	27.281305820155527
65-69	27.08655529233962	22.835533906953703	22.781225651601993	27.296685149104682
70-74	27.007977891128526	22.892448828228996	22.802266333103937	27.29730694753854
75-79	27.015854909716357	22.88201944925765	22.817197641347477	27.28492799967851
80-84	26.98474869636157	22.888704461240845	22.810129775306756	27.316417067090832
85-89	26.952404316693073	22.876556311926137	22.784815247800932	27.38622412357986
90-94	26.568383567797344	22.461609366845998	23.710983866190862	27.25902319916579
95-99	27.225326344324944	22.679293970893948	22.611917869244515	27.483461815536593
100-104	27.303957219076448	22.721382053962362	22.53113923436559	27.4435214925956
105-109	27.204374303645878	22.728766129882374	22.618760465393226	27.448099101078522
110-114	27.193426111034547	22.645099889174062	22.66653235217107	27.49494164762032
115-119	27.163408043982283	22.727324671484602	22.581201588465042	27.528065696068072
120-124	27.069778865494754	22.807008922133445	22.684035487738168	27.43917672463363
125-129	27.02560107207522	22.83014431274405	22.711796169776992	27.432458445403746
130-134	27.16488686307146	22.848998042993998	22.605204204682884	27.38091088925166
135-139	27.252754251791345	22.837835508581826	22.591376734826465	27.318033504800365
140-144	27.22936916075447	22.931277464509993	22.654925765909077	27.18442760882646
145-149	27.157629142006407	22.95512639048415	22.68441467526001	27.202829792249428
150-154	27.078642061866137	23.07292326840522	22.732757206360645	27.115677463367998
155-159	27.02576823462332	23.10218356977932	22.7885937986256	27.083454396971756
160-164	26.994362294275376	23.202085376961183	22.790126910268675	27.01342541849477
165-169	26.869866484553828	23.23747837898926	22.878978345229346	27.01367679122757
170-174	26.846756565420314	23.34272458938855	22.90819385813999	26.902324987051145
175-178	26.821942162970757	23.365177817173482	22.839255685879913	26.97362433397585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	1.0
12	1.5
13	3.5
14	9.5
15	20.0
16	33.5
17	59.5
18	118.5
19	206.5
20	368.0
21	627.0
22	970.5
23	1458.0
24	2131.0
25	2915.0
26	3898.0
27	5042.0
28	6353.0
29	7943.5
30	9747.5
31	11839.5
32	14294.0
33	17294.5
34	20692.0
35	24476.5
36	29001.0
37	34713.0
38	41565.5
39	49614.0
40	59201.0
41	71278.0
42	86193.5
43	103536.0
44	124443.5
45	148874.0
46	175780.0
47	205190.5
48	235806.0
49	266098.5
50	293844.5
51	316910.5
52	335179.5
53	346673.0
54	351920.5
55	352686.0
56	349555.0
57	343764.0
58	334828.0
59	322684.5
60	308387.5
61	291171.0
62	270645.0
63	247673.5
64	221583.0
65	194103.0
66	166102.0
67	137545.0
68	110937.0
69	87781.0
70	67155.5
71	49293.5
72	35607.0
73	24808.5
74	16609.5
75	10898.0
76	6811.0
77	4164.0
78	2487.0
79	1407.0
80	758.5
81	396.5
82	210.0
83	111.5
84	44.0
85	16.0
86	5.0
87	4.0
88	3.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1313.0
92-93	2583.0
94-95	3974.0
96-97	5134.0
98-99	6427.0
100-101	7563.0
102-103	8795.0
104-105	10104.0
106-107	11461.0
108-109	12381.0
110-111	13399.0
112-113	14092.0
114-115	14971.0
116-117	15877.0
118-119	16731.0
120-121	17471.0
122-123	17988.0
124-125	18983.0
126-127	19743.0
128-129	20528.0
130-131	21585.0
132-133	24061.0
134-135	105915.0
136-137	91373.0
138-139	99268.0
140-141	89022.0
142-143	92671.0
144-145	92759.0
146-147	96502.0
148-149	98526.0
150-151	97555.0
152-153	93996.0
154-155	92009.0
156-157	91556.0
158-159	94890.0
160-161	99245.0
162-163	101485.0
164-165	104015.0
166-167	101989.0
168-169	106900.0
170-171	107375.0
172-173	116112.0
174-175	116934.0
176-177	306862.0
178-179	4683608.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.077578293108
#Duplication Level	Percentage of total
1	88.75863074049239
2	9.694064124518757
3	1.1636952551068778
4	0.2314693640151297
5	0.08007628404152986
6	0.032040985971246425
7	0.019770874103330262
8	0.0020951908089257465
9	0.0031639483009069116
>10	0.014993232640911886
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2218746516808718E-4	1.3576385018676355E-5	0.0	0.0	0.0	0.0
2	6.923956359524941E-4	1.3576385018676355E-5	0.0	0.0	0.0	0.0
3	6.923956359524941E-4	1.3576385018676355E-5	1.3576385018676355E-5	0.0	0.0	0.0
4	6.923956359524941E-4	1.3576385018676355E-5	1.3576385018676355E-5	0.0	0.0	0.0
5	7.059720209711705E-4	2.715277003735271E-5	6.788192509338176E-5	0.0	0.0	0.0
6	7.059720209711705E-4	5.430554007470542E-5	9.503469513073448E-5	0.0	0.0	0.0
7	7.059720209711705E-4	8.145831011205812E-5	9.503469513073448E-5	1.3576385018676355E-5	0.0	0.0
8	7.195484059898468E-4	8.145831011205812E-5	1.0861108014941084E-4	1.3576385018676355E-5	0.0	0.0
9	7.195484059898468E-4	8.145831011205812E-5	1.0861108014941084E-4	1.3576385018676355E-5	0.0	0.0
10-14	7.304095140047877E-4	1.2490274217182246E-4	1.5477078921291045E-4	1.3576385018676355E-5	0.0	0.0
15-19	7.765692230682875E-4	1.3576385018676353E-4	2.0364577528014533E-4	2.715277003735271E-5	0.0	0.0
20-24	7.874303310832286E-4	1.3576385018676353E-4	2.986804704108798E-4	4.072915505602906E-5	2.715277003735271E-6	0.0
25-29	8.010067161019049E-4	1.6291662022411627E-4	3.285485174519678E-4	5.430554007470542E-5	1.9006939026146897E-5	0.0
30-34	8.145831011205811E-4	1.8192355925026314E-4	3.4212490247064413E-4	5.430554007470542E-5	4.072915505602906E-5	8.145831011205813E-6
35-39	9.096177962513158E-4	1.9006939026146896E-4	3.5298601048558523E-4	5.430554007470542E-5	5.159026307097015E-5	4.072915505602907E-5
40-44	0.0010345205384231383	1.9821522127267478E-4	3.8828461153414377E-4	5.973609408217596E-5	7.059720209711704E-5	1.0589580314567554E-4
45-49	0.001172999665613637	2.226527143062922E-4	4.91465137676084E-4	7.059720209711704E-5	1.3576385018676353E-4	1.2218746516808718E-4
50-54	0.001262603806736901	2.470902073399096E-4	5.484859547545247E-4	9.50346951307345E-5	1.7377772823905731E-4	1.30333296179293E-4
55-59	0.0013277704548265473	2.769582543809976E-4	6.027914948292301E-4	1.3304857318302828E-4	2.145068832950864E-4	1.4390968119796935E-4
60-64	0.0013875065489087235	2.959651934071445E-4	6.625275889114061E-4	1.3576385018676353E-4	2.3351382232123327E-4	1.5477078921291045E-4
65-69	0.001436381534975958	3.1225685542955614E-4	7.304095140047878E-4	1.4119440419423408E-4	2.498054843436449E-4	1.846388362539984E-4
70-74	0.0018382425315287784	3.1225685542955614E-4	7.602775610458757E-4	1.9006939026146896E-4	2.8510408539220345E-4	2.1722216029882168E-4
75-79	0.008482525359668985	3.1225685542955614E-4	7.819997770757579E-4	2.1179160629135113E-4	3.1225685542955614E-4	2.2536799131002747E-4
80-84	0.011284691227523786	3.1225685542955614E-4	8.091525471131107E-4	2.525207613473802E-4	3.80138780522938E-4	2.3079854531749805E-4
85-89	0.011368864814639579	3.774235035192026E-4	8.145831011205811E-4	2.796735313847329E-4	4.4802070561631975E-4	2.4165965333243906E-4
90-94	0.011504628664826341	4.1543738157149646E-4	8.390205941541986E-4	3.068263014220856E-4	4.615970906349961E-4	2.4437493033617437E-4
95-99	0.011556218927897311	4.5616653662752555E-4	9.04187242243845E-4	3.1225685542955614E-4	4.615970906349961E-4	2.4437493033617437E-4
100-104	0.011588802251942138	5.159026307097014E-4	9.23194181269992E-4	3.1225685542955614E-4	4.7245819864993714E-4	2.4437493033617437E-4
105-109	0.011607809190968283	5.919303868142891E-4	9.829302753521683E-4	3.2040268644076194E-4	4.7517347565367233E-4	2.4437493033617437E-4
110-114	0.011648538346024312	6.190831568516417E-4	0.0010182288764007267	3.258332404482325E-4	4.8331930666487813E-4	2.498054843436449E-4
115-119	0.011705559163102754	6.245137108591123E-4	0.0010399510924306086	3.258332404482325E-4	4.887498606723487E-4	2.579513153548507E-4
120-124	0.011814170243252164	6.489512038927298E-4	0.0010806802474866378	3.366943484631736E-4	5.023262456910251E-4	2.579513153548507E-4
125-129	0.011971656309468809	6.625275889114062E-4	0.0011186941255389316	3.4212490247064413E-4	5.240484617209072E-4	2.579513153548507E-4
130-134	0.012188878469767631	6.951109129562294E-4	0.0011729996656136368	3.5298601048558523E-4	5.512012317582599E-4	2.6338186936232126E-4
135-139	0.012357225643999218	7.059720209711705E-4	0.0012110135436659308	3.584165644930558E-4	6.163678798479065E-4	2.7152770037352705E-4
140-144	0.012601600574335393	7.059720209711705E-4	0.001243596867710754	3.774235035192026E-4	7.086872979749058E-4	2.7152770037352705E-4
145-149	0.012737364424522155	7.41270622019729E-4	0.0012653190837406364	3.801387805229379E-4	7.195484059898468E-4	3.0139574741461505E-4
150-154	0.012813392180626746	7.602775610458758E-4	0.0012897565767742536	3.801387805229379E-4	7.331247910085232E-4	3.1225685542955614E-4
155-159	0.012851406058679038	7.738539460645521E-4	0.0013060482387966651	3.937151655416143E-4	7.711386690608168E-4	3.1225685542955614E-4
160-164	0.0129871699088658	7.874303310832286E-4	0.001344062116848959	3.937151655416143E-4	7.982914390981697E-4	3.149721324332914E-4
165-167	0.013110262466368467	7.874303310832285E-4	0.001416469503615233	4.163424739060748E-4	8.191085627934734E-4	3.3488416379401674E-4
>>END_MODULE
