##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293854
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7718788
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.95630078193623	41.0	40.0	41.0	38.0	41.0
2	40.07643583422682	41.0	40.0	41.0	38.0	41.0
3	40.09412811959598	41.0	40.0	41.0	38.0	41.0
4	40.09460578526059	41.0	40.0	41.0	38.0	41.0
5	40.07978273272954	41.0	40.0	41.0	38.0	41.0
6	40.064161238785154	41.0	40.0	41.0	38.0	41.0
7	40.06317325984338	41.0	40.0	41.0	38.0	41.0
8	40.035769475725985	41.0	40.0	41.0	38.0	41.0
9	40.002492360199554	41.0	40.0	41.0	38.0	41.0
10-14	39.92674735981866	41.0	40.0	41.0	38.0	41.0
15-19	39.76564800069648	40.6	40.0	41.0	37.8	41.0
20-24	39.56808548181398	40.0	39.0	41.0	37.0	41.0
25-29	39.47056734813807	40.0	39.0	41.0	36.4	41.0
30-34	39.25646080705935	40.0	38.6	41.0	35.6	41.0
35-39	38.84671738620104	40.0	37.6	41.0	35.0	41.0
40-44	38.39945066505259	39.4	36.6	41.0	35.0	41.0
45-49	37.623499000102086	38.0	35.2	40.2	34.2	41.0
50-54	36.8024174261555	36.6	35.0	39.2	34.0	41.0
55-59	35.77583491086943	35.2	34.6	37.4	33.4	39.6
60-64	35.42359626925885	35.0	34.8	36.2	33.6	38.8
65-69	35.24247348158804	35.0	35.0	36.0	34.0	37.0
70-74	35.03667886720039	35.0	35.0	35.0	33.2	36.6
75-79	34.948339247042405	35.0	35.0	35.0	33.0	36.0
80-84	34.81635173812261	35.0	34.8	35.0	32.6	36.0
85-89	39.91010166362906	41.0	40.0	41.0	38.0	41.0
90-94	39.851929883377736	41.0	40.0	41.0	38.0	41.0
95-99	39.698697600868464	41.0	39.8	41.0	37.0	41.0
100-104	39.53362801178283	40.4	39.0	41.0	36.4	41.0
105-109	39.301767090474485	40.0	38.6	41.0	35.6	41.0
110-114	39.094827337865524	40.0	38.0	41.0	35.0	41.0
115-119	38.5099444670404	39.4	37.4	40.4	34.6	40.6
120-124	38.50239565272609	39.8	36.8	40.8	35.0	41.0
125-129	37.94129090065694	38.8	35.4	40.8	34.2	41.0
130-134	37.15224593140073	37.2	35.0	39.6	34.0	41.0
135-139	36.45436348850376	36.0	35.0	38.8	34.0	40.8
140-144	35.85966359181235	35.0	35.0	37.0	34.0	39.2
145-149	35.29305819633517	35.0	35.0	36.0	34.0	37.4
150-154	34.91936144397297	35.0	35.0	35.2	33.8	36.4
155-159	34.707695775584675	35.0	35.0	35.0	33.0	36.0
160-164	34.59844244173534	35.0	35.0	35.0	33.0	36.0
165-168	34.364037234153884	35.0	34.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	14.0
30	151.0
31	1893.0
32	12658.0
33	54853.0
34	171360.0
35	445213.0
36	1146410.0
37	2761730.0
38	2698953.0
39	423952.0
40	1601.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.61886037030684	27.426844732618644	29.237504644511546	21.716790252562966
2	21.38296841421218	28.089461713419254	28.854270903670372	21.673298968698194
3	21.354596084255714	28.250756465911486	28.71658089326977	21.678066556563024
4	21.854402530552726	28.235935486244735	28.229535517752268	21.680126465450275
5	21.506005865169506	28.666585997698085	28.150170726285005	21.677237410847404
6	21.457734038038094	28.584526482655047	28.382370392864786	21.575369086442066
7	22.0869908591867	28.26250701535008	27.97576510716449	21.67473701829873
8	21.705169257142444	28.496624081397236	27.91486176327164	21.88334489818868
9	21.502857702530502	28.52897371970833	28.263582313700024	21.704586264061145
10-14	21.636637772665864	28.485062680825017	28.30564073012499	21.572658816384127
15-19	21.463538058047455	28.22465133127118	28.635578539014155	21.676232071667208
20-24	21.48815850364073	28.281346760657243	28.430533394621023	21.79996134108101
25-29	21.64492922982209	28.3135383430663	28.278074226160893	21.76345820095072
30-34	21.552748954887736	28.282870315909697	28.344050905401208	21.820329823801355
35-39	21.53516070139509	28.335088358431403	28.32934134218999	21.800409597983517
40-44	21.713289702994825	28.23385225763423	28.223161460063416	21.829696579307527
45-49	21.75106506358252	28.22621634380942	28.209050436415666	21.8136681561924
50-54	21.69616007072613	28.25890282256748	28.20009566268694	21.84484144401945
55-59	21.7458414455741	28.253220583335104	28.168183916956913	21.832754054133886
60-64	21.6802119711022	28.220456372166204	28.241928655120468	21.857403001611132
65-69	21.616593174990687	28.265592992060412	28.247027901271547	21.870785931677357
70-74	21.67538997054978	28.237767380060184	28.21277381889488	21.87406883049515
75-79	21.61746377799209	28.215896070730274	28.251813626698908	21.91482652457873
80-84	21.56448914000488	28.235023425957547	28.258887276085314	21.94160015795226
85-89	21.534860136073174	28.23726730155045	28.59960657035794	21.628265992018434
90-94	21.701406034743716	28.50347263244501	28.170209691506386	21.62491164130489
95-99	21.499908231970903	28.55885910085177	28.398415489322797	21.542817177854534
100-104	21.55915926788918	28.28344794237377	28.53095576862501	21.62643702111204
105-109	21.564602337275744	28.337180991807493	28.363858796668218	21.73435787424854
110-114	21.581016274620566	28.390029225095763	28.279179106288417	21.74977539399525
115-119	21.617618459152308	28.34463891463904	28.295471833399166	21.74227079280949
120-124	21.596971842509426	28.356112945132374	28.31884166369617	21.72807354866203
125-129	21.640323799876153	28.35412734023364	28.20780752317646	21.797741336713745
130-134	21.805091509258805	28.339676232041406	28.147825253640413	21.707407005059373
135-139	21.717103609642166	28.377606072575322	28.17040460631417	21.73488571146834
140-144	21.642715641268055	28.462952611021414	28.235219578096952	21.659112169613575
145-149	21.722153549527636	28.43046348850123	28.20480182001073	21.642581141960406
150-154	21.63118325779867	28.497957867005336	28.27065417748275	21.600204697713245
155-159	21.570001755466876	28.531671725049545	28.29220993046918	21.606116589014395
160-164	21.59094517003577	28.558977477902523	28.30159295291396	21.548484399147743
165-168	21.4869003795715	28.608321995869858	28.35616531161139	21.54861231294725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	5.0
12	10.0
13	19.0
14	37.5
15	88.0
16	163.5
17	322.5
18	691.0
19	1373.5
20	2617.0
21	4720.5
22	8067.5
23	13138.0
24	20016.5
25	29114.5
26	40610.5
27	54124.5
28	69252.5
29	86196.5
30	104789.0
31	124616.5
32	144927.0
33	165635.5
34	187807.5
35	210313.5
36	233600.5
37	258198.0
38	282349.0
39	305523.0
40	326439.5
41	344887.0
42	359059.0
43	368041.0
44	372419.5
45	370793.0
46	362624.0
47	349256.0
48	330847.5
49	306907.0
50	279253.5
51	249834.0
52	220065.5
53	190040.0
54	161656.5
55	136267.5
56	113196.0
57	93251.5
58	77036.5
59	63177.0
60	51796.5
61	42963.0
62	35893.0
63	30215.5
64	25691.5
65	21837.5
66	18376.0
67	15313.0
68	12562.5
69	10291.5
70	8256.0
71	6436.5
72	4887.0
73	3624.5
74	2653.0
75	1871.5
76	1247.5
77	835.0
78	534.0
79	326.5
80	192.5
81	108.0
82	67.0
83	40.0
84	17.5
85	7.5
86	2.0
87	1.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	821.0
92-93	1945.0
94-95	3121.0
96-97	4116.0
98-99	4827.0
100-101	5776.0
102-103	6776.0
104-105	8281.0
106-107	8221.0
108-109	8685.0
110-111	8895.0
112-113	9374.0
114-115	9919.0
116-117	10382.0
118-119	11002.0
120-121	11738.0
122-123	12282.0
124-125	12927.0
126-127	13549.0
128-129	47783.0
130-131	82165.0
132-133	83953.0
134-135	88510.0
136-137	73643.0
138-139	71588.0
140-141	80715.0
142-143	75890.0
144-145	68533.0
146-147	67490.0
148-149	67542.0
150-151	69154.0
152-153	71194.0
154-155	74740.0
156-157	77320.0
158-159	80687.0
160-161	78866.0
162-163	82420.0
164-165	88501.0
166-167	178049.0
168-169	5957408.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.19865345494296
#Duplication Level	Percentage of total
1	78.03350710594765
2	14.228453191869555
3	3.9736047232060345
4	1.5599331039834237
5	0.792200801163786
6	0.4584181807791345
7	0.27372033297981246
8	0.16070114434400787
9	0.1123753217215621
>10	0.39063072632953344
>50	0.005885756081526455
>100	0.01056961159394538
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.5910803613209744E-5	1.2955401806604872E-5	0.0	0.0
2	0.0	1.2955401806604872E-5	2.5910803613209744E-5	1.2955401806604872E-5	0.0	0.0
3	0.0	3.886620541981461E-5	3.886620541981461E-5	2.5910803613209744E-5	0.0	0.0
4	1.2955401806604872E-5	3.886620541981461E-5	5.182160722641949E-5	2.5910803613209744E-5	0.0	0.0
5	1.2955401806604872E-5	3.886620541981461E-5	5.182160722641949E-5	2.5910803613209744E-5	0.0	0.0
6	1.2955401806604872E-5	5.182160722641949E-5	5.182160722641949E-5	2.5910803613209744E-5	1.2955401806604872E-5	0.0
7	1.2955401806604872E-5	7.773241083962922E-5	6.477700903302436E-5	2.5910803613209744E-5	1.2955401806604872E-5	0.0
8	1.2955401806604872E-5	7.773241083962922E-5	6.477700903302436E-5	3.886620541981461E-5	1.2955401806604872E-5	0.0
9	2.5910803613209744E-5	7.773241083962922E-5	7.773241083962922E-5	3.886620541981461E-5	1.2955401806604872E-5	0.0
10-14	3.627512505849364E-5	1.1400753589812286E-4	9.327889300755508E-5	5.700376794906143E-5	1.2955401806604872E-5	0.0
15-19	5.700376794906143E-5	1.943310270990731E-4	1.502826609566165E-4	8.03234912009502E-5	1.813756252924682E-5	0.0
20-24	6.736808939434534E-5	2.7206343793870224E-4	1.710113038471843E-4	1.0882537517548092E-4	6.477700903302435E-5	0.0
25-29	8.291457156227117E-5	3.238850451651218E-4	1.9433102709907305E-4	1.425094198726536E-4	8.809673228491312E-5	0.0
30-34	1.0882537517548092E-4	4.0939069708871405E-4	2.4874371468681353E-4	1.9951318782171503E-4	1.295540180660487E-4	0.0
35-39	1.9951318782171498E-4	4.2752825961796075E-4	2.953831611905911E-4	2.3060615215756672E-4	2.2542399143492473E-4	2.0728642890567796E-5
40-44	2.9020100046794905E-4	4.7157662576041736E-4	3.4979584877833155E-4	2.6169911649341843E-4	3.523869291396525E-4	4.145728578113559E-5
45-49	4.3271042034060265E-4	5.363536347934417E-4	4.0420853636607203E-4	3.0315640227455395E-4	4.663944650377754E-4	5.182160722641949E-5
50-54	5.596733580453305E-4	5.778109205745773E-4	4.378925810632446E-4	3.187028844424798E-4	5.959484831038241E-4	7.773241083962922E-5
55-59	6.503611706915645E-4	6.140860456330709E-4	4.6380338467645436E-4	3.290672058877637E-4	7.177292600859098E-4	7.773241083962922E-5
60-64	7.02182777917984E-4	6.399968492462806E-4	4.897141882896642E-4	3.497958487783315E-4	8.524654388746006E-4	1.0364321445283896E-4
65-69	0.0015442838953473007	6.710898135821323E-4	5.415357955160837E-4	3.523869291396525E-4	9.509264926047976E-4	1.1659861625944382E-4
70-74	0.016339352758490064	7.306846618925148E-4	5.467179562387255E-4	4.01617456004751E-4	0.0010701161892255624	1.502826609566165E-4
75-79	0.0226564066793906	7.565954655057245E-4	5.648555187679723E-4	4.327104203406027E-4	0.0012774026181312402	1.6842022348586332E-4
80-84	0.02269268180444909	7.669597869510084E-4	5.829930812972192E-4	4.8194094720570117E-4	0.0016401538687161768	1.6842022348586332E-4
85-89	0.02271600152770098	8.343278763453538E-4	5.98539563465145E-4	5.493090366000466E-4	0.0016634735919680652	1.6842022348586332E-4
90-94	0.02274450341167551	8.861494835717733E-4	6.296325278009967E-4	5.933574027425031E-4	0.0016919754759425962	1.6842022348586332E-4
95-99	0.02276264097420476	9.586997336887605E-4	6.555433314142066E-4	6.1149496527175E-4	0.0017204773599171267	1.6842022348586332E-4
100-104	0.022773005295650045	0.001002748099831217	6.659076528594904E-4	6.659076528594904E-4	0.0017567524849756203	1.6842022348586332E-4
105-109	0.02278596069745665	0.0010519786266963156	7.099560190019469E-4	6.995916975566631E-4	0.0017878454493114725	1.6842022348586332E-4
110-114	0.02279114285817929	0.001093435912477451	7.565954655057245E-4	7.332757422538357E-4	0.001826711654731287	1.6842022348586332E-4
115-119	0.022881830670825522	0.001108982394645377	8.058259923708229E-4	7.695508673123293E-4	0.001896670824486953	1.6842022348586332E-4
120-124	0.02309170818009252	0.0011245288768133026	8.861494835717733E-4	7.876884298415761E-4	0.0019614478335199777	1.710113038471843E-4
125-129	0.02340263782345104	0.0011918969662076482	9.690640551340444E-4	7.954616709255392E-4	0.0021013661730313097	1.813756252924682E-4
130-134	0.02376279799367465	0.0012489007341567097	9.97565939108575E-4	8.291457156227117E-4	0.0022179647892907545	1.9173994673775207E-4
135-139	0.023900125252824665	0.0013214509842736967	0.0010364321445283896	8.835584032104521E-4	0.0022671953161558524	1.9433102709907307E-4
140-144	0.024135913565704874	0.0013680904307774744	0.0010830715910321673	8.991048853783781E-4	0.002319016923382272	1.9951318782171503E-4
145-149	0.024322471351719988	0.001388819073668042	0.0011219377964519818	9.250156889915878E-4	0.0023812028520539754	2.098775092669989E-4
150-154	0.024496073735928492	0.0014406406808944617	0.0011426664393425496	9.690640551340444E-4	0.002425251218196432	2.2283291107360378E-4
155-157	0.024783683656035116	0.0014639604041463505	0.0011789415644010432	9.716551354953653E-4	0.0024485709414483205	2.3319723251888767E-4
>>END_MODULE
