##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293855
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7660004
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.97491554834697	41.0	40.0	41.0	38.0	41.0
2	40.08875008420361	41.0	40.0	41.0	38.0	41.0
3	40.09912801612114	41.0	40.0	41.0	38.0	41.0
4	40.101880625649805	41.0	40.0	41.0	38.0	41.0
5	40.08854225141397	41.0	40.0	41.0	38.0	41.0
6	40.07008638637787	41.0	40.0	41.0	38.0	41.0
7	40.06625375130353	41.0	40.0	41.0	38.0	41.0
8	40.039878699802244	41.0	40.0	41.0	38.0	41.0
9	40.01376370038449	41.0	40.0	41.0	38.0	41.0
10-14	39.93258275060953	41.0	40.0	41.0	38.0	41.0
15-19	39.77815768764612	40.6	40.0	41.0	38.0	41.0
20-24	39.58107933102907	40.0	39.2	41.0	37.0	41.0
25-29	39.48710141665723	40.0	39.0	41.0	36.6	41.0
30-34	39.26752576108315	40.0	38.6	41.0	35.6	41.0
35-39	38.86444419611269	40.0	37.6	41.0	35.0	41.0
40-44	38.40917952523263	39.4	36.6	41.0	35.0	41.0
45-49	37.631510244642165	38.0	35.2	40.2	34.4	41.0
50-54	36.8088063922682	36.6	35.0	39.2	34.0	41.0
55-59	35.792829742647655	35.4	34.6	37.4	33.4	39.6
60-64	35.431596067051665	35.0	34.8	36.2	33.6	38.8
65-69	35.242579377243146	35.0	35.0	36.0	34.0	37.0
70-74	35.036187291808204	35.0	35.0	35.0	33.4	36.6
75-79	34.943434990373376	35.0	35.0	35.0	33.0	36.0
80-84	34.81206610335973	35.0	34.8	35.0	32.6	36.0
85-89	39.91888500319321	41.0	40.0	41.0	38.0	41.0
90-94	39.86416617394899	41.0	40.0	41.0	38.0	41.0
95-99	39.70733781539229	41.0	39.8	41.0	37.0	41.0
100-104	39.5443393315986	40.4	39.0	41.0	36.4	41.0
105-109	39.31545206956698	40.0	38.6	41.0	35.6	41.0
110-114	39.10935361944755	40.0	38.0	41.0	35.0	41.0
115-119	38.52961805866311	39.4	37.4	40.4	34.6	40.6
120-124	38.519708287691266	39.8	36.8	40.8	35.0	41.0
125-129	37.96027030430806	38.8	35.4	40.8	34.4	41.0
130-134	37.16241154373213	37.2	35.0	39.6	34.0	41.0
135-139	36.4606473545442	36.0	35.0	38.8	34.0	40.8
140-144	35.85981002275038	35.0	35.0	37.0	34.0	39.2
145-149	35.2981756261446	35.0	35.0	36.0	34.0	37.4
150-154	34.922682035661595	35.0	35.0	35.2	34.0	36.4
155-159	34.71518498622435	35.0	35.0	35.0	33.0	36.0
160-164	34.601165108771404	35.0	35.0	35.0	33.0	36.0
165-168	34.37094684806202	35.0	34.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	138.0
31	1815.0
32	12187.0
33	53188.0
34	167054.0
35	436057.0
36	1128156.0
37	2744931.0
38	2693469.0
39	421531.0
40	1475.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.620302025951947	27.421708918167663	29.228574815365633	21.729414240514757
2	21.41752145299141	28.05271381059331	28.821969283566958	21.707795452848327
3	21.362116782184447	28.299045274649988	28.67749155222373	21.661346390941834
4	21.848983368677093	28.250259399342347	28.20985472070249	21.69090251127806
5	21.553291617080095	28.660076417714663	28.132726823641345	21.653905141563897
6	21.443200290757026	28.58179969618815	28.39080762882108	21.58419238423374
7	22.124179047426086	28.25177375886488	27.929332673977715	21.69471451973132
8	21.721816333255177	28.51106866262733	27.891904495089037	21.875210509028456
9	21.527195024963433	28.4876613641455	28.246995693474837	21.73814791741623
10-14	21.64290253634332	28.46911045999454	28.30819409493781	21.57979290872433
15-19	21.4691193372745	28.2249408747045	28.617321870850198	21.6886179171708
20-24	21.511740724939568	28.282517868136885	28.397426424320404	21.808314982603143
25-29	21.652048745666452	28.299870339493292	28.271554427386725	21.77652648745353
30-34	21.573876462727696	28.27985468414899	28.32094082457398	21.825328028549333
35-39	21.5442185147684	28.317656230988913	28.32934029799462	21.808784956248065
40-44	21.72989987994784	28.231016589547476	28.19112888191703	21.84795464858765
45-49	21.758155739866456	28.230726772466436	28.1996902351487	21.811427252518406
50-54	21.713174040117995	28.24387559066549	28.175870404245217	21.867079964971296
55-59	21.756730153143522	28.232554447752246	28.162463100541462	21.848252298562766
60-64	21.711573518760567	28.2043586400216	28.21876594320316	21.865301898014675
65-69	21.626547453500024	28.25848916005788	28.232849486762667	21.882113899679425
70-74	21.706727568288475	28.198987885646016	28.22481554839919	21.86946899766632
75-79	21.62280333012881	28.216917380199803	28.227935651208536	21.93234363846285
80-84	21.583367319390433	28.239295958592187	28.231773769308738	21.94556295270864
85-89	21.53411408140257	28.226444790368255	28.596389766898294	21.64305136133088
90-94	21.720533824875282	28.493601378215715	28.144960725798025	21.640904071110985
95-99	21.519469730459246	28.54609993027474	28.388103392858817	21.546326946407206
100-104	21.559721747077848	28.271310107333935	28.516704505436753	21.652263640151464
105-109	21.584157444254377	28.330477059180552	28.345952261231904	21.739413235333167
110-114	21.597329165666572	28.389854064573527	28.253996042021534	21.75882072773837
115-119	21.643452634139212	28.35450333422599	28.270050122717116	21.731993908917687
120-124	21.59674602375206	28.35558421159173	28.306363726197333	21.74130603845888
125-129	21.644835501025582	28.35292265477966	28.200812329451637	21.801429514743116
130-134	21.81594151437705	28.33850576038251	28.128514318467086	21.71703840677335
135-139	21.74138873047379	28.354165761294002	28.16260884434236	21.74183666388985
140-144	21.646691976755204	28.435978640903038	28.240828041690875	21.676501340650887
145-149	21.726655420359872	28.401784509700377	28.21213033187916	21.65942973806059
150-154	21.637441579364467	28.490976908363443	28.25054986156222	21.62103165070987
155-159	21.584198077551527	28.538261417462763	28.26490539016185	21.61263511482386
160-164	21.614578264966784	28.53965035966148	28.267806791901418	21.57796458347032
165-168	21.507239502351013	28.595788946459738	28.338459058371345	21.5585124928179
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.5
11	8.0
12	12.5
13	26.5
14	40.5
15	71.5
16	157.0
17	321.0
18	653.5
19	1306.5
20	2545.0
21	4588.5
22	7825.5
23	12690.0
24	19562.5
25	28890.5
26	40160.5
27	53066.5
28	68270.5
29	85280.0
30	103616.5
31	123094.5
32	143470.5
33	164657.0
34	185656.5
35	207543.5
36	231336.0
37	255502.5
38	279368.5
39	302696.0
40	323529.0
41	341250.5
42	356118.5
43	366096.0
44	370042.0
45	368189.0
46	360530.0
47	347711.5
48	328789.0
49	304638.0
50	277522.5
51	248761.5
52	218712.5
53	188701.0
54	160937.0
55	135518.5
56	112880.0
57	93105.5
58	76483.5
59	63155.0
60	52047.5
61	42804.0
62	35819.0
63	30168.0
64	25400.0
65	21558.0
66	18278.5
67	15337.0
68	12634.5
69	10243.5
70	8207.5
71	6466.5
72	4967.5
73	3629.0
74	2589.5
75	1879.0
76	1322.5
77	858.5
78	538.0
79	339.5
80	209.0
81	128.0
82	61.5
83	29.5
84	15.0
85	5.5
86	3.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	760.0
92-93	1907.0
94-95	2898.0
96-97	3955.0
98-99	4928.0
100-101	5588.0
102-103	6351.0
104-105	7170.0
106-107	7706.0
108-109	8253.0
110-111	8676.0
112-113	8973.0
114-115	9611.0
116-117	10027.0
118-119	10706.0
120-121	11044.0
122-123	11570.0
124-125	12636.0
126-127	12956.0
128-129	46176.0
130-131	80473.0
132-133	81514.0
134-135	82952.0
136-137	71993.0
138-139	68937.0
140-141	74030.0
142-143	71737.0
144-145	66922.0
146-147	65480.0
148-149	66837.0
150-151	67367.0
152-153	69659.0
154-155	71162.0
156-157	74062.0
158-159	77007.0
160-161	78067.0
162-163	80962.0
164-165	84797.0
166-167	174155.0
168-169	5960000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.16830876889739
#Duplication Level	Percentage of total
1	77.97989818950704
2	14.200375589075481
3	4.110211239963924
4	1.5987375226686695
5	0.7728704698361137
6	0.3966882493853097
7	0.23334240943058268
8	0.18371308521193322
9	0.10716119994730489
>10	0.39278370858097866
>50	0.009890309255130205
>100	0.007665616985361241
>500	0.006662410152185439
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.3054823470066073E-5	2.6109646940132145E-5	1.3054823470066073E-5	0.0
2	0.0	5.221929388026429E-5	1.3054823470066073E-5	2.6109646940132145E-5	1.3054823470066073E-5	0.0
3	2.6109646940132145E-5	6.527411735033037E-5	1.3054823470066073E-5	2.6109646940132145E-5	1.3054823470066073E-5	0.0
4	2.6109646940132145E-5	6.527411735033037E-5	3.916447041019822E-5	2.6109646940132145E-5	1.3054823470066073E-5	0.0
5	2.6109646940132145E-5	6.527411735033037E-5	5.221929388026429E-5	6.527411735033037E-5	1.3054823470066073E-5	0.0
6	2.6109646940132145E-5	7.832894082039644E-5	6.527411735033037E-5	6.527411735033037E-5	1.3054823470066073E-5	0.0
7	2.6109646940132145E-5	9.138376429046252E-5	6.527411735033037E-5	6.527411735033037E-5	2.6109646940132145E-5	0.0
8	2.6109646940132145E-5	1.0443858776052858E-4	9.138376429046252E-5	6.527411735033037E-5	2.6109646940132145E-5	0.0
9	2.6109646940132145E-5	1.1749341123059466E-4	9.138376429046252E-5	6.527411735033037E-5	2.6109646940132145E-5	0.0
10-14	4.699736449223787E-5	1.4099209347671356E-4	1.2010437592460788E-4	1.0443858776052858E-4	3.916447041019822E-5	0.0
15-19	6.527411735033037E-5	1.9321138735697792E-4	1.6710174041684574E-4	1.4882498755875324E-4	8.616183490243608E-5	0.0
20-24	9.921665837250217E-5	2.6109646940132146E-4	2.219319989911232E-4	1.6449077572283253E-4	1.2010437592460788E-4	0.0
25-29	1.3577016408868715E-4	3.3159251613967827E-4	2.6370743409533467E-4	1.9843331674500433E-4	1.3838112878270035E-4	0.0
30-34	1.9060042266296466E-4	3.550911983857972E-4	2.7415129287138755E-4	2.6370743409533467E-4	1.6449077572283253E-4	0.0
35-39	3.4203637491573115E-4	3.7858988063191616E-4	2.7937322225941397E-4	2.9503901042349326E-4	2.4020875184921575E-4	5.221929388026429E-6
40-44	5.221929388026429E-4	4.4125303328823324E-4	3.342034808336915E-4	3.263705867516518E-4	3.054828691995461E-4	2.3498682246118928E-5
45-49	6.919056439135019E-4	4.908613624744843E-4	3.89033739407969E-4	3.681460218558633E-4	4.203653157361276E-4	3.916447041019822E-5
50-54	7.389030084057397E-4	5.326367975786959E-4	4.0469952757204834E-4	3.785898806319161E-4	5.039161859445504E-4	7.04960467383568E-5
55-59	8.485635255542948E-4	6.187986324811319E-4	4.203653157361276E-4	4.0469952757204823E-4	5.848560914589601E-4	9.921665837250217E-5
60-64	9.060047488225855E-4	6.501302088092905E-4	4.4125303328823324E-4	4.4125303328823324E-4	6.475192441152773E-4	1.1488244653658144E-4
65-69	0.0016919051217205634	7.049604673835679E-4	4.7780653900441825E-4	4.6475171553435216E-4	7.441249377937661E-4	1.3054823470066073E-4
70-74	0.016966048581697868	7.310701143236999E-4	4.830284683924447E-4	5.248039034966561E-4	8.276758080021891E-4	1.4099209347671356E-4
75-79	0.023913825632467033	7.624016906518586E-4	4.830284683924447E-4	5.848560914589601E-4	9.295034310687044E-4	1.436030581707268E-4
80-84	0.023976488785123345	7.885113375919907E-4	4.8825039778047113E-4	6.344644206452112E-4	0.0011305477125077219	1.436030581707268E-4
85-89	0.024002598432063482	8.563964196363344E-4	5.326367975786958E-4	6.892946792194886E-4	0.0011357696418957484	1.436030581707268E-4
90-94	0.024049595796555717	9.347253604567309E-4	5.587464445188279E-4	7.310701143236999E-4	0.001146213500671801	1.436030581707268E-4
95-99	0.024067872549413814	9.738898308669291E-4	6.161876677871186E-4	7.78067478815938E-4	0.0011514354300598277	1.436030581707268E-4
100-104	0.024130535702070133	0.0010391639482172596	6.657959969733697E-4	8.224538786141626E-4	0.0011644902535298937	1.436030581707268E-4
105-109	0.024182754995950396	0.0010809393833214709	6.945166086075151E-4	8.642293137183741E-4	0.0011827670063879862	1.436030581707268E-4
110-114	0.024232363325136647	0.0011671012182239069	7.154043261596209E-4	9.60835007396863E-4	0.0011905999004700259	1.436030581707268E-4
115-119	0.02433941287759119	0.0012480411237383165	7.336810790177133E-4	9.97388513113048E-4	0.0012558740178203562	1.436030581707268E-4
120-124	0.024459517253515795	0.001279372700066475	8.015661610620568E-4	0.0010287200894412065	0.001399477075991083	1.436030581707268E-4
125-129	0.02465272864087277	0.0013237590998647	8.433415961662683E-4	0.0010809393833214709	0.001545691098855823	1.5665788164079288E-4
130-134	0.024898159322110015	0.0013707564643569378	9.033937841285722E-4	0.0011253257831196953	0.0016005213574301006	1.6971270511085895E-4
135-139	0.025010430803952582	0.0013812003231329906	9.347253604567307E-4	0.0011905999004700259	0.0016631845100864178	1.6971270511085895E-4
140-144	0.025198420261921532	0.0014281976876252284	9.451692192327837E-4	0.0012193205121041714	0.0017441244156008273	1.749346344988854E-4
145-149	0.025436018049076737	0.0014673621580354266	9.634459720908763E-4	0.001229764370880224	0.0017937327447870785	1.8276752858092504E-4
150-154	0.02557439917785944	0.0015143595225276646	9.738898308669291E-4	0.0012402082296562769	0.0018746726503014882	1.8537849327493824E-4
155-157	0.02578762796120385	0.0015665788164079288	9.921665837250215E-4	0.0012663178765964093	0.0019190590500997127	1.958223520509911E-4
>>END_MODULE
