##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293856
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7666942
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.97851451073974	41.0	40.0	41.0	38.0	41.0
2	40.08970551753228	41.0	40.0	41.0	38.0	41.0
3	40.10424808222105	41.0	40.0	41.0	38.0	41.0
4	40.106195664451356	41.0	40.0	41.0	38.0	41.0
5	40.09510297586704	41.0	40.0	41.0	38.0	41.0
6	40.07323102222503	41.0	40.0	41.0	38.0	41.0
7	40.07185707156778	41.0	40.0	41.0	38.0	41.0
8	40.046816840403906	41.0	40.0	41.0	38.0	41.0
9	40.00857212693144	41.0	40.0	41.0	38.0	41.0
10-14	39.93839995137566	41.0	40.0	41.0	38.0	41.0
15-19	39.78362645758896	40.6	40.0	41.0	38.0	41.0
20-24	39.585220782940574	40.0	39.2	41.0	37.0	41.0
25-29	39.487262379185864	40.0	39.0	41.0	36.8	41.0
30-34	39.27132314291669	40.0	38.6	41.0	35.6	41.0
35-39	38.85760570511685	40.0	37.6	41.0	35.0	41.0
40-44	38.39343592269252	39.4	36.4	41.0	35.0	41.0
45-49	37.5990936934178	38.0	35.2	40.2	34.4	41.0
50-54	36.77014971549282	36.4	35.0	39.0	34.0	41.0
55-59	35.7510855044945	35.2	34.6	37.4	33.4	39.6
60-64	35.3999083337268	35.0	34.8	36.0	33.6	38.8
65-69	35.22059692117144	35.0	35.0	36.0	34.0	37.0
70-74	35.0256076020922	35.0	35.0	35.0	33.4	36.4
75-79	34.93681872120593	35.0	35.0	35.0	33.0	36.0
80-84	34.80960578546179	35.0	34.8	35.0	32.6	36.0
85-89	39.93422412742916	41.0	40.0	41.0	38.0	41.0
90-94	39.88160712748642	41.0	40.0	41.0	38.0	41.0
95-99	39.731194811233095	41.0	40.0	41.0	37.0	41.0
100-104	39.56806863691743	40.6	39.0	41.0	36.6	41.0
105-109	39.330307883260296	40.0	38.6	41.0	35.6	41.0
110-114	39.12604705169615	40.0	38.0	41.0	35.0	41.0
115-119	38.55315022237109	39.4	37.4	40.4	34.8	40.6
120-124	38.55874456841238	40.0	36.8	40.8	35.0	41.0
125-129	38.006166495257716	39.0	35.6	41.0	34.4	41.0
130-134	37.230150791860346	37.2	35.0	39.8	34.0	41.0
135-139	36.5301519799426	36.2	35.0	39.0	34.0	40.8
140-144	35.92783889581168	35.2	35.0	37.0	34.0	39.2
145-149	35.34701702866173	35.0	35.0	36.0	34.0	37.6
150-154	34.95650739158769	35.0	35.0	35.4	34.0	36.4
155-159	34.73563836428669	35.0	35.0	35.0	33.2	36.0
160-164	34.61823963087114	35.0	35.0	35.0	33.0	36.0
165-168	34.383844225697985	35.0	34.75	35.0	32.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	130.0
31	1856.0
32	12585.0
33	53226.0
34	164271.0
35	429970.0
36	1116257.0
37	2738109.0
38	2715015.0
39	433885.0
40	1632.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.61194124071892	27.430897481681743	29.222367927134442	21.734793350464894
2	21.434138930488842	28.046957965770446	28.831651524167	21.687251579573708
3	21.367984784546433	28.310674581860667	28.67877440575395	21.642566227838948
4	21.886353124883428	28.217482276506068	28.194330934028194	21.701833664582306
5	21.51780983865536	28.678239642350235	28.119803175764208	21.684147343230194
6	21.477037389874607	28.57900842343662	28.390680404260266	21.55327378242851
7	22.111305915709288	28.250233274231107	27.960613240585356	21.677847569474242
8	21.724267641518612	28.491933289700118	27.889698396048907	21.89410067273236
9	21.504271194434494	28.504167632936312	28.270358638424554	21.721202534204643
10-14	21.639931539849915	28.481389320540053	28.294730806624074	21.583948332985955
15-19	21.48592228818217	28.20483577415872	28.62597630189455	21.68326563576456
20-24	21.511752664882557	28.268018722458056	28.40236954968487	21.81785906297452
25-29	21.669408742103435	28.286894566308185	28.265446640916288	21.778250050672092
30-34	21.579028509671783	28.26961257826132	28.315936132032824	21.835422780034072
35-39	21.549266969803607	28.335273698431525	28.302887904982192	21.81257142678267
40-44	21.72557715970722	28.229283591815356	28.19077280094202	21.854366447535405
45-49	21.767862597630188	28.224188992169235	28.19148755788162	21.816460852318954
50-54	21.723401585664792	28.246787311029614	28.17512640632993	21.854684696975664
55-59	21.749722379535413	28.256642609269772	28.161326901912133	21.832308109282682
60-64	21.705574399806338	28.19242665459058	28.23202523248513	21.869973713117954
65-69	21.63677513146702	28.25333490197265	28.219230039825526	21.890659926734806
70-74	21.6968486262189	28.217195330289442	28.205746176246016	21.880209867245636
75-79	21.614051599712113	28.205954864403566	28.252706228898038	21.92728730698628
80-84	21.580278030015094	28.213728498272197	28.245949949797456	21.960043521915257
85-89	21.546950531254836	28.23474600433915	28.58230569632586	21.635997768080156
90-94	21.712487164295734	28.49827549758076	28.155707414375524	21.63352992374798
95-99	21.51437927727137	28.55083698555894	28.38777805038259	21.547005686787095
100-104	21.578850053586475	28.264098944328126	28.527070192791008	21.629980809294388
105-109	21.580143383629615	28.33491147812089	28.339094586180135	21.745850552069356
110-114	21.58856213064806	28.38649050899185	28.27544545347563	21.749501906884458
115-119	21.65734058416775	28.340559890751944	28.258152542872818	21.743946982207493
120-124	21.61402658624832	28.340296360582222	28.3110679135531	21.734609139616357
125-129	21.681252675430922	28.32238162170066	28.194367256387697	21.80199844648072
130-134	21.828288562498965	28.32374160374555	28.132661982533907	21.715307851221578
135-139	21.75332159501422	28.337661762150603	28.164507949909616	21.74450869292556
140-144	21.65356264006351	28.447983804265174	28.230096559576346	21.668356996094968
145-149	21.724734820307177	28.40211172130204	28.20373701784068	21.6694164405501
150-154	21.643791794829102	28.484400544274706	28.25692866585236	21.614878995043828
155-159	21.590501582166823	28.52142897458037	28.275381351552415	21.61268809170039
160-164	21.605005836269285	28.55749541964577	28.271856772331986	21.565641971752957
165-168	21.51567920025494	28.59340839831774	28.33862118968425	21.552291211743064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	3.5
12	15.5
13	28.0
14	42.5
15	79.5
16	169.0
17	353.0
18	695.5
19	1316.0
20	2497.0
21	4606.0
22	7961.0
23	12836.5
24	19701.0
25	28587.5
26	39612.5
27	52950.5
28	68150.5
29	84687.5
30	103259.5
31	123097.5
32	143337.5
33	164363.0
34	185923.0
35	208355.0
36	231631.0
37	255756.5
38	279304.0
39	302261.5
40	324280.5
41	342254.5
42	356402.0
43	366885.5
44	370884.0
45	368936.0
46	361176.0
47	347669.0
48	328870.5
49	305203.5
50	278405.0
51	248821.0
52	218823.5
53	189849.0
54	161604.0
55	135605.5
56	112549.0
57	92728.0
58	76703.0
59	63228.0
60	51929.5
61	42868.5
62	35681.5
63	30100.5
64	25524.5
65	21689.0
66	18341.0
67	15250.5
68	12495.5
69	10101.0
70	8115.5
71	6378.0
72	4869.0
73	3646.0
74	2638.0
75	1909.5
76	1333.5
77	858.5
78	550.5
79	324.0
80	180.5
81	116.5
82	73.0
83	39.5
84	20.0
85	8.5
86	2.5
87	1.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	742.0
92-93	1831.0
94-95	2899.0
96-97	3833.0
98-99	4646.0
100-101	5346.0
102-103	6117.0
104-105	7008.0
106-107	7489.0
108-109	8138.0
110-111	8340.0
112-113	8844.0
114-115	9264.0
116-117	9939.0
118-119	10310.0
120-121	10986.0
122-123	11366.0
124-125	12164.0
126-127	12691.0
128-129	45433.0
130-131	79435.0
132-133	81032.0
134-135	82149.0
136-137	70566.0
138-139	67681.0
140-141	72900.0
142-143	72495.0
144-145	66682.0
146-147	64680.0
148-149	65743.0
150-151	66827.0
152-153	69813.0
154-155	70042.0
156-157	72211.0
158-159	76028.0
160-161	76621.0
162-163	79397.0
164-165	89580.0
166-167	170015.0
168-169	5985659.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.07099010976252
#Duplication Level	Percentage of total
1	77.87744323926259
2	14.1412451422082
3	4.14226085207547
4	1.6352322837016338
5	0.7922603190291924
6	0.43683763936111475
7	0.2737657343439015
8	0.16490997783368835
9	0.12816164668848776
>10	0.37835932833795816
>50	0.014494563570159462
>100	0.007940214661150719
>500	0.007089058926494548
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.3043009846689853E-5	0.0	0.0	1.3043009846689853E-5	0.0
2	0.0	1.3043009846689853E-5	1.3043009846689853E-5	2.6086019693379706E-5	1.3043009846689853E-5	0.0
3	0.0	1.3043009846689853E-5	1.3043009846689853E-5	2.6086019693379706E-5	2.6086019693379706E-5	0.0
4	0.0	2.6086019693379706E-5	3.912902954006956E-5	2.6086019693379706E-5	2.6086019693379706E-5	0.0
5	0.0	3.912902954006956E-5	3.912902954006956E-5	2.6086019693379706E-5	2.6086019693379706E-5	0.0
6	2.6086019693379706E-5	3.912902954006956E-5	3.912902954006956E-5	2.6086019693379706E-5	2.6086019693379706E-5	0.0
7	2.6086019693379706E-5	5.217203938675941E-5	6.521504923344927E-5	2.6086019693379706E-5	2.6086019693379706E-5	0.0
8	3.912902954006956E-5	7.825805908013912E-5	6.521504923344927E-5	2.6086019693379706E-5	3.912902954006956E-5	0.0
9	3.912902954006956E-5	1.0434407877351882E-4	6.521504923344927E-5	2.6086019693379706E-5	3.912902954006956E-5	0.0
10-14	4.173763150940753E-5	1.2260429255888462E-4	7.043225317212521E-5	6.521504923344926E-5	4.173763150940753E-5	0.0
15-19	5.217203938675942E-5	1.4608171028292636E-4	1.1216988468153272E-4	1.1216988468153275E-4	6.260644726411129E-5	0.0
20-24	7.304085514146317E-5	2.139053614857136E-4	1.3564730240557448E-4	1.5651611816027824E-4	1.4086450634425042E-4	0.0
25-29	9.651827286550492E-5	2.399913811790933E-4	1.6173332209895418E-4	1.7999353588431997E-4	1.7216772997630608E-4	2.6086019693379705E-6
30-34	1.512989142216023E-4	2.921634205658527E-4	1.7999353588431997E-4	2.295569733017414E-4	1.9564514770034782E-4	1.3043009846689855E-5
35-39	2.68686002841811E-4	2.9998922647386664E-4	2.1912256542438953E-4	2.4520858511776925E-4	2.478171870871072E-4	3.1303223632055645E-5
40-44	3.57378469799302E-4	3.3390105207526023E-4	2.895548185965147E-4	2.9738062450452863E-4	3.9911610130870955E-4	7.043225317212521E-5
45-49	5.060687820515663E-4	3.860730914620196E-4	3.208580422285704E-4	3.130322363205565E-4	4.6954835448083474E-4	8.869246695749101E-5
50-54	6.15630064763761E-4	3.965074993393716E-4	3.52161265860626E-4	3.3390105207526023E-4	5.269375978062701E-4	1.3043009846689852E-4
55-59	6.678021041505205E-4	4.3302792691010314E-4	3.730300816153298E-4	3.391182560139362E-4	6.025870549170711E-4	1.4347310831358838E-4
60-64	7.669289789853635E-4	4.538967426648069E-4	4.147677131247373E-4	3.678128776766539E-4	6.469332883958167E-4	1.4869031225226432E-4
65-69	0.001622550424928218	5.399806076529599E-4	4.3302792691010314E-4	3.9129029540069563E-4	7.591031730773494E-4	1.7477633194564406E-4
70-74	0.01708634289916371	6.130214627944231E-4	4.48679538726131E-4	4.486795387261309E-4	8.556214459428544E-4	2.0607955557769967E-4
75-79	0.023837404795810375	6.547590943038307E-4	4.669397525114968E-4	4.7737416038884866E-4	0.0010251805739498224	2.2173116739372748E-4
80-84	0.023902619845043827	6.756279100585343E-4	4.747655584195107E-4	5.217203938675942E-4	0.0013851676457184623	2.2173116739372748E-4
85-89	0.023936531670645217	7.147569395986039E-4	5.086773840209043E-4	5.373720056836219E-4	0.001421688073289194	2.2694837133240345E-4
90-94	0.023980877904123964	8.138838144334468E-4	5.217203938675942E-4	5.765010352236915E-4	0.0014347310831358839	2.3477417724041737E-4
95-99	0.024035658545480062	8.999676794215998E-4	5.243289958369321E-4	6.26064472641113E-4	0.0014399482870745598	2.3477417724041737E-4
100-104	0.02406435316714278	9.364881069923314E-4	5.765010352236915E-4	6.756279100585343E-4	0.0014921203264613193	2.3477417724041737E-4
105-109	0.024098264992744173	0.0010043117581951186	6.182386667330991E-4	7.12148337629266E-4	0.0015364665599400646	2.3477417724041737E-4
110-114	0.024189566061671	0.0010930042251526097	6.860623179358863E-4	7.434515612613216E-4	0.00161994182295888	2.3477417724041737E-4
115-119	0.024317387558168566	0.0011947397019567904	7.04322531721252E-4	7.721461829240393E-4	0.0016668966584069632	2.4259998314843126E-4
120-124	0.0245260757157156	0.001231260129527522	7.225827455066179E-4	8.399698341268266E-4	0.0017321117076404126	2.478171870871072E-4
125-129	0.024758241290986676	0.0013121267905769993	7.95623600648081E-4	8.89533271544248E-4	0.0018155869706592276	2.6868600284181096E-4
130-134	0.02496432084656438	0.0013695160339024346	8.243182223107987E-4	9.234450971456415E-4	0.001865150408076649	3.0259782844320465E-4
135-139	0.025123445566694	0.0013982106555651523	8.608386498815304E-4	9.625741266857111E-4	0.0019277568553407605	3.391182560139362E-4
140-144	0.025248658461222217	0.001424296675258532	8.89533271544248E-4	9.756171365324012E-4	0.0019799288947275192	3.52161265860626E-4
145-149	0.025462563822707934	0.0014582085008599257	9.260536991149796E-4	9.78225738501739E-4	0.002047752545930307	3.52161265860626E-4
150-154	0.025720815417672394	0.001518206346154699	9.521397188083593E-4	0.0010121375641031325	0.0021599224306118403	3.5998707176863995E-4
155-157	0.02604254299389074	0.001569508851551679	9.521397188083594E-4	0.0010608314675307747	0.0021912256542438954	3.7389961560510917E-4
>>END_MODULE
