##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293857
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7747081
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.961682600194834	41.0	40.0	41.0	38.0	41.0
2	40.08645359458614	41.0	40.0	41.0	38.0	41.0
3	40.104697885564896	41.0	40.0	41.0	38.0	41.0
4	40.10289204411313	41.0	40.0	41.0	38.0	41.0
5	40.088054068364585	41.0	40.0	41.0	38.0	41.0
6	40.074383629137216	41.0	40.0	41.0	38.0	41.0
7	40.068791716518774	41.0	40.0	41.0	38.0	41.0
8	40.044441383793455	41.0	40.0	41.0	38.0	41.0
9	40.00970533288603	41.0	40.0	41.0	38.0	41.0
10-14	39.93355156606727	41.0	40.0	41.0	38.0	41.0
15-19	39.7759531105974	40.6	40.0	41.0	37.8	41.0
20-24	39.58210637012831	40.0	39.0	41.0	37.0	41.0
25-29	39.48960866679979	40.0	39.0	41.0	36.8	41.0
30-34	39.27679937772691	40.0	38.6	41.0	35.6	41.0
35-39	38.87433576594849	40.0	37.6	41.0	35.0	41.0
40-44	38.42316717741818	39.4	36.6	41.0	35.0	41.0
45-49	37.64894256301179	38.0	35.2	40.4	34.4	41.0
50-54	36.83415725226056	36.6	35.0	39.2	34.0	41.0
55-59	35.79048939852313	35.2	34.6	37.4	33.4	39.6
60-64	35.442534342935105	35.0	34.8	36.2	33.6	39.0
65-69	35.26151010942056	35.0	35.0	36.0	34.0	37.2
70-74	35.05757807359959	35.0	35.0	35.0	33.4	36.6
75-79	34.96582619957117	35.0	35.0	35.0	33.0	36.0
80-84	34.834464619641906	35.0	34.8	35.0	32.6	36.0
85-89	39.91772606998687	41.0	40.0	41.0	38.0	41.0
90-94	39.864050885408886	41.0	40.0	41.0	38.0	41.0
95-99	39.71561649900708	41.0	39.8	41.0	37.0	41.0
100-104	39.54946356662512	40.6	39.0	41.0	36.4	41.0
105-109	39.31685407850799	40.0	38.6	41.0	35.6	41.0
110-114	39.110731424402175	40.0	38.0	41.0	35.0	41.0
115-119	38.51180858422647	39.4	37.4	40.4	34.6	40.6
120-124	38.52630362022399	40.0	36.8	40.8	35.0	41.0
125-129	37.98363364423124	38.8	35.6	41.0	34.4	41.0
130-134	37.18920871219471	37.2	35.0	39.8	34.0	41.0
135-139	36.489646481472086	36.0	35.0	38.8	34.0	40.8
140-144	35.891620482993616	35.2	35.0	37.0	34.0	39.2
145-149	35.318374671219054	35.0	35.0	36.0	34.0	37.4
150-154	34.941721493075605	35.0	35.0	35.2	34.0	36.4
155-159	34.72762193881929	35.0	35.0	35.0	33.2	36.0
160-164	34.61493859217804	35.0	35.0	35.0	33.0	36.0
165-168	34.37645763210589	35.0	34.75	35.0	32.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	8.0
30	153.0
31	1920.0
32	12457.0
33	54413.0
34	167807.0
35	435802.0
36	1124693.0
37	2747584.0
38	2748260.0
39	451787.0
40	2197.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.579134128067075	27.422715729963326	29.28812800589022	21.71002213607938
2	21.380013969132374	28.076858884010637	28.871261317649832	21.671865829207153
3	21.36492441475699	28.29246267077884	28.701507057948668	21.641105856515505
4	21.83649557814098	28.250872812611615	28.25206035666853	21.660571252578876
5	21.528508608597225	28.677756176810338	28.161277776752303	21.632457437840134
6	21.41585972832864	28.654573251525317	28.38855047468847	21.541016545457573
7	22.06876370596874	28.2680405690866	27.996570579293028	21.666625145651633
8	21.6950358464046	28.50609152014804	27.944615526802934	21.854257106644425
9	21.50625506561762	28.503703523946633	28.26397452150042	21.726066888935332
10-14	21.621495889871294	28.489793768775616	28.32934365859864	21.559366682754447
15-19	21.44759813405849	28.24489636806431	28.652510539130805	21.6549949587464
20-24	21.47002206379409	28.29478612654237	28.451880133949807	21.78331167571373
25-29	21.64674669078586	28.3196367767421	28.280664678735125	21.75295185373691
30-34	21.535982391303254	28.310311974277795	28.359179412219902	21.794526222199046
35-39	21.513093770414947	28.352343289040093	28.35021345459019	21.784349485954774
40-44	21.694945489791575	28.248853987714856	28.239668592596363	21.81653192989721
45-49	21.73375236427759	28.26875825875578	28.219369334075633	21.778120042890993
50-54	21.667482242666626	28.275816401041887	28.21796235252994	21.838739003761546
55-59	21.715430624773383	28.270333045439955	28.20332199960217	21.81091433018449
60-64	21.656500041757663	28.235398597226492	28.26970313076628	21.838398230249563
65-69	21.590033717215555	28.282234302184268	28.282464066143103	21.84526791445707
70-74	21.659391453374504	28.241798427046266	28.240352721237844	21.85845739834139
75-79	21.588433114356235	28.244351646768635	28.271492191704205	21.89572304717093
80-84	21.552897149261767	28.253051697794305	28.264142326638897	21.92990882630503
85-89	21.519875679626946	28.255592009429098	28.61104976183933	21.613482549104624
90-94	21.688541006133807	28.5228867430408	28.17407246243259	21.614499788392802
95-99	21.466256274905245	28.594141339644185	28.429303571996666	21.5102988134539
100-104	21.534133783348064	28.30218511244992	28.543906131933984	21.61977497226804
105-109	21.56096940147272	28.354112257785104	28.38654996876388	21.6983683719783
110-114	21.56748637313545	28.42112889913912	28.29352990139074	21.71785482633469
115-119	21.631558566147465	28.366848807430895	28.29954859223312	21.70204403418852
120-124	21.57788265259192	28.381903959895734	28.334855999908175	21.70535738760417
125-129	21.62529365781229	28.369069627946864	28.2276938875755	21.777942826665353
130-134	21.801630339318358	28.373954153444554	28.13680755028873	21.68760795694836
135-139	21.705142986696092	28.385386987978034	28.205339764593916	21.704130260731958
140-144	21.62559548431629	28.481924976299037	28.255835977176147	21.636643562208526
145-149	21.698712067868243	28.448296282199063	28.241117701167877	21.611873948764813
150-154	21.604135952347367	28.51741458644653	28.29557336697677	21.582876094229324
155-159	21.543774000115867	28.547663702223918	28.321474652501	21.58708764515921
160-164	21.587619581353902	28.579186566124104	28.313494674413587	21.51969917810841
165-168	21.488562277508326	28.613254158525493	28.36905370418496	21.529129859781225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	1.0
11	3.5
12	10.5
13	23.5
14	45.5
15	78.0
16	167.0
17	340.0
18	707.5
19	1372.0
20	2548.5
21	4712.5
22	8284.0
23	13298.5
24	20209.0
25	29125.5
26	40447.5
27	54379.0
28	70280.0
29	87578.5
30	106061.0
31	125730.5
32	145954.5
33	167283.5
34	189286.0
35	212073.0
36	235708.5
37	259825.5
38	284439.5
39	307722.5
40	328446.5
41	346284.0
42	360282.5
43	369604.0
44	374063.0
45	372718.5
46	364420.0
47	350961.5
48	331468.0
49	306503.0
50	279255.0
51	250173.5
52	219784.0
53	189946.5
54	161911.0
55	136203.5
56	113212.5
57	93014.0
58	76419.0
59	62572.5
60	51500.5
61	42815.5
62	35664.0
63	29984.5
64	25250.5
65	21321.0
66	18057.5
67	15099.0
68	12429.0
69	10068.5
70	8068.5
71	6323.5
72	4786.0
73	3562.0
74	2625.0
75	1861.0
76	1278.0
77	820.0
78	521.5
79	332.0
80	206.5
81	122.0
82	59.0
83	34.5
84	17.5
85	8.0
86	5.5
87	2.5
88	0.5
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	808.0
92-93	2022.0
94-95	3186.0
96-97	4144.0
98-99	5153.0
100-101	6350.0
102-103	7370.0
104-105	7444.0
106-107	8130.0
108-109	8541.0
110-111	8986.0
112-113	9406.0
114-115	9826.0
116-117	10653.0
118-119	11200.0
120-121	11999.0
122-123	12239.0
124-125	12631.0
126-127	13262.0
128-129	47243.0
130-131	90691.0
132-133	87037.0
134-135	84577.0
136-137	75455.0
138-139	71952.0
140-141	77143.0
142-143	75205.0
144-145	69217.0
146-147	67711.0
148-149	73676.0
150-151	69905.0
152-153	70841.0
154-155	73492.0
156-157	76688.0
158-159	78081.0
160-161	80064.0
162-163	82423.0
164-165	88184.0
166-167	202193.0
168-169	5951953.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.01283638574445
#Duplication Level	Percentage of total
1	77.76140525386117
2	14.325443229118893
3	3.9933422311046436
4	1.6542762234934925
5	0.8043547356315253
6	0.4665752803512534
7	0.2788483604931143
8	0.18257445019152943
9	0.1154980420062484
>10	0.3946064286837068
>50	0.008734610320565241
>100	0.007750919512103059
>500	0.0065902352317289865
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.2908087575178316E-5	2.5816175150356632E-5	0.0
2	0.0	1.2908087575178316E-5	0.0	1.2908087575178316E-5	3.872426272553495E-5	0.0
3	0.0	1.2908087575178316E-5	0.0	1.2908087575178316E-5	3.872426272553495E-5	0.0
4	2.5816175150356632E-5	3.872426272553495E-5	2.5816175150356632E-5	1.2908087575178316E-5	3.872426272553495E-5	0.0
5	2.5816175150356632E-5	5.1632350300713265E-5	2.5816175150356632E-5	3.872426272553495E-5	5.1632350300713265E-5	0.0
6	2.5816175150356632E-5	5.1632350300713265E-5	2.5816175150356632E-5	3.872426272553495E-5	5.1632350300713265E-5	0.0
7	2.5816175150356632E-5	6.454043787589158E-5	5.1632350300713265E-5	5.1632350300713265E-5	5.1632350300713265E-5	0.0
8	2.5816175150356632E-5	6.454043787589158E-5	5.1632350300713265E-5	6.454043787589158E-5	6.454043787589158E-5	0.0
9	2.5816175150356632E-5	9.035661302624821E-5	5.1632350300713265E-5	7.74485254510699E-5	6.454043787589158E-5	0.0
10-14	3.614264521049929E-5	1.2133602320667618E-4	5.421396781574892E-5	1.0842793563149784E-4	6.454043787589158E-5	0.0
15-19	6.97036729059629E-5	1.5231543338710413E-4	6.454043787589158E-5	1.3166249326681883E-4	8.777499551121256E-5	0.0
20-24	9.293823054128388E-5	2.1943748877803135E-4	8.261176048114122E-5	1.7813160853746078E-4	1.187544056916405E-4	0.0
25-29	1.4973381587206846E-4	2.8397792665392296E-4	1.5231543338710413E-4	2.400904288983167E-4	1.4198896332696148E-4	0.0
30-34	2.2976395883817402E-4	3.278654244095292E-4	1.7554999102242512E-4	2.9946763174413697E-4	1.8071322605249643E-4	0.0
35-39	4.285485074959201E-4	3.9756909731549216E-4	2.1685587126299568E-4	3.381918944696719E-4	2.865595441689586E-4	1.0326470060142653E-5
40-44	5.963536459732382E-4	4.7243600525152633E-4	2.659066040486733E-4	4.2080365495081307E-4	3.7433453968017114E-4	3.0979410180427956E-5
45-49	6.841286414844506E-4	5.653742357928103E-4	2.9172277919902996E-4	4.3371174252599136E-4	4.956705628868473E-4	4.905073278567761E-5
50-54	7.66740401965592E-4	6.324962911837375E-4	3.5884483458995724E-4	4.621095351913837E-4	6.505676137889871E-4	5.1632350300713265E-5
55-59	8.132095172362338E-4	6.634757013641654E-4	4.2338527246584874E-4	5.034154154319544E-4	7.667404019655919E-4	8.003014296610557E-5
60-64	8.622602500219115E-4	6.81547023969415E-4	4.67272770221455E-4	5.085786504620257E-4	8.570970149918401E-4	9.035661302624821E-5
65-69	0.0017271021175588586	7.383426093001997E-4	4.905073278567761E-4	5.318132080973467E-4	9.551984805631953E-4	1.0068308308639087E-4
70-74	0.017699569683084507	8.028830471760913E-4	5.395580606424537E-4	5.808639408830242E-4	0.0010300653884992297	1.2133602320667618E-4
75-79	0.024447917867387727	8.364440748715549E-4	5.550477657326676E-4	6.324962911837375E-4	0.0011746359693412267	1.2908087575178316E-4
80-84	0.024527948010353837	8.545153974768044E-4	5.808639408830242E-4	6.789654064543794E-4	0.0014379609558748644	1.2908087575178316E-4
85-89	0.02457183550810944	9.242190703827674E-4	6.092617335484165E-4	6.918734940295577E-4	0.0014663587485402563	1.2908087575178316E-4
90-94	0.024610559770834976	9.758514206834806E-4	6.479859962739514E-4	7.176896691799143E-4	0.0015128278638108985	1.2908087575178316E-4
95-99	0.02463637594598533	0.001063626416194693	6.892918765145221E-4	7.693220194806276E-4	0.0015360624214462197	1.2908087575178316E-4
100-104	0.02465186565107555	0.0011307484715856204	7.254345217250213E-4	8.157911347512696E-4	0.0015360624214462197	1.2908087575178316E-4
105-109	0.024667355356165762	0.0011617278817660484	7.460874618453066E-4	8.570970149918401E-4	0.0015515521265364336	1.2908087575178316E-4
110-114	0.024706079618891295	0.0012495028772772608	7.925565771159486E-4	9.242190703827674E-4	0.0016496535921077886	1.2908087575178316E-4
115-119	0.024801599466947617	0.001370838900483937	8.261176048114122E-4	9.629433331083024E-4	0.0017374285876190013	1.2908087575178316E-4
120-124	0.025005547250635436	0.0014689403660552926	8.674234850519827E-4	0.0010042492133488727	0.0018019690254948927	1.2908087575178316E-4
125-129	0.025276617089714182	0.0015592969790815406	9.009845127474464E-4	0.0010429734760744078	0.0019310499012466758	1.3940734581192584E-4
130-134	0.025537360458732776	0.0016006028593221113	9.138926003226248E-4	0.0011049322964352638	0.0020084984266977458	1.6264190344724677E-4
135-139	0.025599319279093636	0.0016444903570777172	9.758514206834806E-4	0.0011359117066156918	0.0020807837171187447	1.678051384773181E-4
140-144	0.025790358975206273	0.0017012859424085022	0.0010223205359541226	0.0011462381766758343	0.0021375793024495287	1.678051384773181E-4
145-149	0.026012378081499345	0.0017348469701039654	0.0010326470060142653	0.0011488197941908701	0.002173721947660028	1.7554999102242512E-4
150-154	0.026226652335247302	0.0018045506430099285	0.0010687896512247645	0.0011720543518261909	0.002240844003050956	1.8071322605249643E-4
155-157	0.02653902805456662	0.001867370002542463	0.0010971874438901567	0.0012133602320667616	0.002280428804948169	1.8931861776928197E-4
>>END_MODULE
