##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293863
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7441443
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.35311229287115	39.0	39.0	39.0	37.0	39.0
2	40.162848522793226	41.0	40.0	41.0	38.0	41.0
3	40.20553742063199	41.0	40.0	41.0	38.0	41.0
4	40.22521384629298	41.0	40.0	41.0	38.0	41.0
5	40.232164783093815	41.0	40.0	41.0	38.0	41.0
6	40.20686619517209	41.0	40.0	41.0	38.0	41.0
7	40.23084124947272	41.0	40.0	41.0	38.0	41.0
8	40.22263504538031	41.0	40.0	41.0	38.0	41.0
9	40.20616754035474	41.0	40.0	41.0	38.0	41.0
10-14	40.14983833108713	41.0	40.0	41.0	38.0	41.0
15-19	40.02504336860471	41.0	40.0	41.0	38.0	41.0
20-24	39.82719023716234	41.0	40.0	41.0	37.6	41.0
25-29	39.53795214180905	40.6	39.0	41.0	36.4	41.0
30-34	39.240457932688585	40.0	38.4	41.0	35.0	41.0
35-39	38.979695981007985	40.0	37.6	41.0	35.0	41.0
40-44	38.41530821374296	39.6	35.8	41.0	35.0	41.0
45-49	37.70278794045724	38.0	35.0	41.0	35.0	41.0
50-54	37.01837538767683	36.6	35.0	39.8	34.2	41.0
55-59	36.39379300493197	35.2	35.0	38.8	34.0	41.0
60-64	35.742839231584526	35.0	34.8	36.8	33.6	39.8
65-69	35.46422660228668	35.0	35.0	36.0	33.6	39.0
70-74	35.303536908096994	35.0	35.0	35.8	34.0	37.8
75-79	35.154083609859	35.0	35.0	35.0	33.6	37.0
80-84	35.09323030492876	35.0	35.0	35.0	33.0	37.0
85-89	34.9083535276693	35.0	34.6	35.0	32.6	37.2
90-94	39.63572545099655	40.6	39.8	40.6	37.8	40.6
95-99	39.89709546861852	41.0	40.0	41.0	37.6	41.0
100-104	39.772964814296714	41.0	40.0	41.0	37.0	41.0
105-109	39.614387692671094	41.0	39.4	41.0	36.0	41.0
110-114	39.407469577084	40.8	39.0	41.0	35.2	41.0
115-119	39.08371458647069	40.0	38.0	41.0	35.0	41.0
120-124	38.749108124575464	40.0	37.4	41.0	35.0	41.0
125-129	38.14714203349621	39.6	35.8	40.6	34.4	41.0
130-134	37.78591397621044	38.8	35.0	41.0	34.0	41.0
135-139	37.38003523208455	37.2	35.0	40.8	34.0	41.0
140-144	36.81014897818483	36.0	35.0	39.4	34.0	41.0
145-149	36.186747121453074	35.0	35.0	38.0	34.0	40.8
150-154	35.64525560201123	35.0	35.0	36.6	34.0	39.2
155-159	35.2143543075289	35.0	35.0	35.8	33.2	37.4
160-164	34.90580116314547	35.0	35.0	35.0	33.0	36.4
165-169	34.69970409622829	35.0	35.0	35.0	33.0	36.0
170-174	34.58174500286608	35.0	35.0	35.0	33.0	35.8
175-178	34.263897732839766	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	37.0
30	444.0
31	3216.0
32	16600.0
33	61832.0
34	180478.0
35	463752.0
36	1299221.0
37	2498690.0
38	2213978.0
39	688184.0
40	15010.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.353857578429345	25.896294576199804	26.755523088734268	23.99432475663658
2	24.181546509191833	26.28746870734614	26.57887724195428	22.952107541507743
3	22.38911189671143	25.74636397806178	27.668733066960264	24.195791058266522
4	23.10604273929129	25.051512186547694	27.875682176158577	23.966762898002443
5	23.875234951070645	25.53992552250954	26.47065898374818	24.11418054267163
6	23.85944500280389	25.776371598895537	26.313014827903675	24.051168570396896
7	23.928974528192988	25.87951019714859	26.080989937032374	24.110525337626047
8	24.0412242625523	25.99830436112996	25.848414077753468	24.11205729856427
9	24.118212018824845	26.10521910871319	25.85585887038307	23.9207100020789
10-14	24.033946104270367	26.070217832750984	25.852684754825106	24.04315130815354
15-19	24.019919254907954	26.020378036894186	25.939971588843726	24.019731119354137
20-24	23.9863612474086	25.931435610001984	25.98771232945008	24.094490813139334
25-29	23.9960206642717	25.908555639007112	25.870993031862234	24.224430664858954
30-34	24.032951673485908	25.945970425359704	25.857202695767473	24.163875205386912
35-39	24.05619447733457	25.933749677313926	25.850763084525408	24.159292760826094
40-44	24.06921883296022	25.90961457341002	25.843699938304976	24.177466655324782
45-49	24.18253825232552	25.86732976386435	25.782112420937715	24.168019562872416
50-54	24.167025132087957	25.90484936859692	25.783281549022146	24.144843950292973
55-59	24.14115111813663	25.860341871865444	25.795814064557103	24.202692945440823
60-64	24.113336620330223	25.902645494966503	25.823225414748187	24.16079246995509
65-69	24.11346025226559	25.887608088915016	25.807040381818418	24.19189127700098
70-74	24.05892244286491	25.897753970567265	25.82388926448808	24.219434322079735
75-79	24.066633312920626	25.885573537283022	25.859460322413273	24.18833282738308
80-84	24.063047986795034	25.90903135319319	25.82005667449176	24.20786398552001
85-89	24.037386297254443	25.874016638977142	25.819712655193356	24.268884408575055
90-94	23.390436587329308	25.746181362528205	26.989973550629298	23.873408499513193
95-99	23.87472542564205	26.027216724127854	26.04454764126783	24.053510208962265
100-104	23.97049216431454	26.072377572848026	25.93168256473894	24.0254476980985
105-109	23.895057104009442	26.037416123224954	26.016513306379196	24.051013466386408
110-114	23.90858598583527	25.92806430582055	26.043997777426608	24.119351930917578
115-119	23.947970333477766	25.972638495151536	25.90320048620218	24.176190685168514
120-124	23.91577291005287	25.970324711253596	25.92213571655914	24.191766662134395
125-129	23.901900085467474	25.985120330248606	25.96151183589366	24.15146774839026
130-134	24.023798582357962	25.964627967217403	25.849965053178632	24.161608397246003
135-139	24.080340936951362	25.920558918944952	25.83066627217585	24.16843387192783
140-144	24.056095572238338	26.024225710580453	25.865901411355967	24.053777305825243
145-149	24.020069820990862	26.01262125826339	25.901703929287677	24.06560499145807
150-154	23.934183614330575	26.097258338254314	25.95624473907801	24.012313308337095
155-159	23.90371554700367	26.14875833967019	25.999929217531324	23.947596895794813
160-164	23.841023379751086	26.233659357386323	26.02614671614743	23.89917054671516
165-169	23.765443319437505	26.234925596385967	26.106167266434362	23.89346381774217
170-174	23.716880113443136	26.366467559592728	26.143185608135393	23.773466718828747
175-178	23.67573240634753	26.41527489024547	26.133447896708127	23.775544806698864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	3.5
12	8.0
13	21.0
14	47.5
15	95.0
16	182.5
17	375.0
18	766.0
19	1522.0
20	2720.5
21	4683.5
22	7664.5
23	11625.0
24	16781.0
25	22802.0
26	30326.0
27	39057.0
28	48287.5
29	58390.0
30	69228.5
31	80528.5
32	92278.5
33	104774.5
34	117981.0
35	131993.0
36	146201.5
37	159996.5
38	173135.5
39	186513.5
40	199632.5
41	212178.5
42	223914.5
43	235012.5
44	246069.0
45	255639.5
46	263663.5
47	270356.0
48	275263.5
49	277993.5
50	277845.0
51	273830.5
52	266305.5
53	255300.5
54	241614.5
55	226883.0
56	210913.5
57	195429.0
58	180980.5
59	166315.5
60	152935.0
61	141279.0
62	129515.5
63	117880.0
64	106830.0
65	95412.0
66	84396.5
67	73608.0
68	62550.5
69	52074.0
70	42457.5
71	33792.5
72	25958.5
73	19243.5
74	13965.5
75	9821.0
76	6691.5
77	4374.0
78	2717.0
79	1602.0
80	914.0
81	505.0
82	250.5
83	114.5
84	47.0
85	31.5
86	20.5
87	6.5
88	1.5
89	2.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	991.0
92-93	2451.0
94-95	2989.0
96-97	3902.0
98-99	5759.0
100-101	5570.0
102-103	6806.0
104-105	7675.0
106-107	8623.0
108-109	9348.0
110-111	11007.0
112-113	11935.0
114-115	11894.0
116-117	12065.0
118-119	12521.0
120-121	12674.0
122-123	13627.0
124-125	13883.0
126-127	14491.0
128-129	15112.0
130-131	16185.0
132-133	18022.0
134-135	84595.0
136-137	75996.0
138-139	71219.0
140-141	74733.0
142-143	70400.0
144-145	69925.0
146-147	91822.0
148-149	77946.0
150-151	72982.0
152-153	74510.0
154-155	77802.0
156-157	70785.0
158-159	73392.0
160-161	77355.0
162-163	74906.0
164-165	78716.0
166-167	83744.0
168-169	87752.0
170-171	84397.0
172-173	89318.0
174-175	99014.0
176-177	254068.0
178-179	5308536.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.80185848302133
#Duplication Level	Percentage of total
1	92.15275220345774
2	6.587769338932715
3	0.7910546661480481
4	0.20088189369364945
5	0.09033783973837684
6	0.05063944381136454
7	0.043695962810481784
8	0.030372640854996344
9	0.010543151576959321
>10	0.041952858975643534
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.719126922023054E-5	0.0	0.0	0.0	0.0	0.0
2	0.0012900723690284263	1.3438253844046107E-5	0.0	2.6876507688092214E-5	0.0	0.0
3	0.0012900723690284263	5.375301537618443E-5	0.0	5.375301537618443E-5	1.3438253844046107E-5	0.0
4	0.0012900723690284263	5.375301537618443E-5	0.0	5.375301537618443E-5	1.3438253844046107E-5	0.0
5	0.0012900723690284263	5.375301537618443E-5	0.0	6.719126922023054E-5	2.6876507688092214E-5	0.0
6	0.0013035106228724725	5.375301537618443E-5	0.0	6.719126922023054E-5	2.6876507688092214E-5	0.0
7	0.0013035106228724725	5.375301537618443E-5	0.0	6.719126922023054E-5	2.6876507688092214E-5	0.0
8	0.0013169488767165185	5.375301537618443E-5	4.031476153213832E-5	9.406777690832276E-5	2.6876507688092214E-5	0.0
9	0.0013169488767165185	6.719126922023054E-5	5.375301537618443E-5	9.406777690832276E-5	2.6876507688092214E-5	0.0
10-14	0.0013276994797917555	9.675542767713197E-5	5.644066614499365E-5	1.2363193536522418E-4	2.687650768809221E-5	0.0
15-19	0.0013303871305605647	1.4513314151569796E-4	1.1019368152117808E-4	1.4513314151569796E-4	2.9564158456901436E-5	0.0
20-24	0.0013492006859422293	1.5857139535974406E-4	1.2094428459641497E-4	1.7738495074140864E-4	6.18159676826121E-5	0.0
25-29	0.0013680142413238939	1.881355538166455E-4	1.2363193536522418E-4	2.0694910919831006E-4	6.987891998903977E-5	0.0
30-34	0.0014352055105441245	2.1501206150473768E-4	1.6932199843498096E-4	2.365132676552115E-4	8.600482460189508E-5	8.062952306427665E-6
35-39	0.001577651001291013	2.3920091842402074E-4	1.8276025227902706E-4	2.7145272764973137E-4	1.2094428459641497E-4	2.6876507688092217E-5
40-44	0.0016824693812745727	2.4188856919282994E-4	2.365132676552115E-4	3.171427907194881E-4	2.042614584295008E-4	6.18159676826121E-5
45-49	0.0017899754120269417	2.7951567995615907E-4	2.580144738056853E-4	3.923970122461463E-4	2.3113796611759306E-4	6.719126922023054E-5
50-54	0.0018464160781719353	3.090798384130605E-4	2.768280291873498E-4	4.595882814663769E-4	2.848909814937775E-4	9.675542767713197E-5
55-59	0.0019216702996985933	3.2789339379472496E-4	3.0907983841306047E-4	5.160289476113706E-4	3.386439968699619E-4	1.3169488767165186E-4
60-64	0.0020103627750692977	3.601452030204357E-4	3.4670694917638957E-4	6.181596768261209E-4	3.628328537892449E-4	1.8544790304783629E-4
65-69	0.002074866393520719	3.950846630149556E-4	4.0045996455257397E-4	7.014768506592068E-4	4.192735199342386E-4	2.015738076606916E-4
70-74	0.0024807016596109116	4.380870753159031E-4	4.4615002762233083E-4	7.498545644977728E-4	4.5690063069756773E-4	2.3113796611759306E-4
75-79	0.008659610777103312	4.73026535310423E-4	4.784018368480415E-4	7.794187229546742E-4	5.106536460737521E-4	2.4188856919282994E-4
80-84	0.011591837765874174	4.945277414608968E-4	5.187165983801797E-4	8.224211352556217E-4	6.477238352830224E-4	2.4188856919282994E-4
85-89	0.011634840178175121	5.321548522242259E-4	5.321548522242258E-4	8.466099921749049E-4	8.439223414060954E-4	2.4188856919282994E-4
90-94	0.011761159764309154	5.45593106068272E-4	5.644066614499365E-4	8.6273589678776E-4	8.573605952501416E-4	2.4188856919282994E-4
95-99	0.01180147452584129	5.644066614499365E-4	5.644066614499365E-4	8.923000552446617E-4	8.600482460189507E-4	2.4188856919282994E-4
100-104	0.01184178928737343	5.83220216831601E-4	5.751572645251734E-4	9.08425959857517E-4	8.654235475565695E-4	2.4188856919282994E-4
105-109	0.011892854651980806	6.047214229820748E-4	5.778449152939826E-4	9.514283721584645E-4	8.788618014006156E-4	2.4188856919282994E-4
110-114	0.011917043508900087	6.154720260573116E-4	6.100967245196932E-4	0.0010105566890722673	9.164889121639446E-4	2.4188856919282994E-4
115-119	0.011960045921201036	6.450361845142131E-4	6.208473275949301E-4	0.0010616220536796427	9.353024675456092E-4	2.4188856919282994E-4
120-124	0.012035300142727695	6.880385968151607E-4	6.369732322077855E-4	0.0011341886244374915	9.917431336906029E-4	2.4188856919282994E-4
125-129	0.01219118388731863	7.041645014280161E-4	6.450361845142131E-4	0.0012121304967329589	0.0010965615136741625	2.768280291873498E-4
130-134	0.012387382393441702	7.337286598849174E-4	6.746003429711145E-4	0.0012524452582650973	0.0011503145290503469	2.8220333072496827E-4
135-139	0.012543266138032638	7.52542215266582E-4	7.014768506592068E-4	0.0012658835121091433	0.0011771910367384393	3.359563461011527E-4
140-144	0.01273140169184928	7.740434214170557E-4	7.364163106537266E-4	0.001308885924410091	0.0012040675444265313	3.493945999451988E-4
145-149	0.012787842357994276	7.982322783363389E-4	7.982322783363389E-4	0.0013276994797917555	0.0012712588136467619	3.493945999451988E-4
150-154	0.012860408928752126	8.11670532180385E-4	8.277964367932402E-4	0.001378764844399131	0.0012927600197972355	3.5476990148281733E-4
155-159	0.012932975499509978	8.38547039868477E-4	8.439223414060954E-4	0.001394890749011986	0.0013276994797917555	3.7089580609567257E-4
160-164	0.013002854419499013	8.466099921749049E-4	8.519852937125233E-4	0.0014002660505496045	0.001408329002856032	3.76271107633291E-4
165-167	0.01313813284152908	8.600482460189508E-4	8.734864998629971E-4	0.0014334137433649182	0.0014244549074688875	3.8522994352932174E-4
>>END_MODULE
