##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293864
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5130906
Total Bases	876.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.366266893215354	39.0	39.0	39.0	37.0	39.0
2	40.15779630342088	41.0	40.0	41.0	38.0	41.0
3	40.19994129691715	41.0	40.0	41.0	38.0	41.0
4	40.208123867402755	41.0	40.0	41.0	38.0	41.0
5	40.20802719831546	41.0	40.0	41.0	38.0	41.0
6	40.19239974382692	41.0	40.0	41.0	38.0	41.0
7	40.21812989752687	41.0	40.0	41.0	38.0	41.0
8	40.20326702535576	41.0	40.0	41.0	38.0	41.0
9	40.184427272688296	41.0	40.0	41.0	38.0	41.0
10-14	40.12356694899498	41.0	40.0	41.0	38.0	41.0
15-19	39.9969742185883	41.0	40.0	41.0	38.0	41.0
20-24	39.79805511931031	41.0	40.0	41.0	37.4	41.0
25-29	39.511701130365665	40.4	39.0	41.0	36.4	41.0
30-34	39.20997172818991	40.0	38.2	41.0	35.0	41.0
35-39	38.97213618803385	40.0	37.6	41.0	35.0	41.0
40-44	38.41811255166242	39.6	35.8	41.0	35.0	41.0
45-49	37.70945758897161	38.2	35.0	41.0	35.0	41.0
50-54	37.01501099415971	36.6	35.0	39.8	34.0	41.0
55-59	36.39958264680742	35.4	35.0	38.8	34.0	41.0
60-64	35.737013073324675	35.0	34.8	36.8	33.6	39.8
65-69	35.4588277391946	35.0	35.0	36.0	33.6	39.0
70-74	35.28602071447031	35.0	35.0	35.8	33.8	37.8
75-79	35.13417918784713	35.0	35.0	35.0	33.0	37.0
80-84	35.06180623850837	35.0	35.0	35.0	33.0	37.0
85-89	34.87102971677906	35.0	34.6	35.0	32.6	37.2
90-94	39.609004527110166	40.6	39.8	40.6	37.8	40.6
95-99	39.858945190435165	41.0	40.0	41.0	37.4	41.0
100-104	39.73445529839208	41.0	40.0	41.0	36.8	41.0
105-109	39.56789625733744	41.0	39.2	41.0	36.0	41.0
110-114	39.35788700728914	40.4	38.8	41.0	35.0	41.0
115-119	39.0347842292386	40.0	38.0	41.0	35.0	41.0
120-124	38.70056038383778	40.0	37.2	41.0	35.0	41.0
125-129	38.09637150289272	39.6	35.8	40.6	34.2	41.0
130-134	37.74932139306137	38.8	35.0	41.0	34.0	41.0
135-139	37.368289520349634	37.4	35.0	40.8	34.0	41.0
140-144	36.81240316783031	36.2	35.0	39.4	34.0	41.0
145-149	36.19097420895255	35.0	35.0	38.0	34.0	40.8
150-154	35.637235458262246	35.0	35.0	36.6	33.4	39.2
155-159	35.190787972458004	35.0	35.0	35.8	33.0	37.6
160-164	34.87110984386719	35.0	35.0	35.0	33.0	36.4
165-169	34.66068471479759	35.0	35.0	35.0	33.0	36.0
170-174	34.53262815200857	35.0	35.0	35.0	33.0	35.8
175-178	34.2125090911251	34.75	34.25	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	31.0
30	307.0
31	2128.0
32	11336.0
33	43303.0
34	127107.0
35	334612.0
36	918385.0
37	1720675.0
38	1504173.0
39	459919.0
40	8927.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.274544495650478	25.941519879724943	26.898836189943843	23.885099434680736
2	24.082296576861864	26.374347922179826	26.68743103069906	22.855924470259247
3	22.29395744143432	25.83590500391159	27.746210903103663	24.123926651550427
4	23.03690615263659	25.088571102257575	27.96013023820744	23.91439250689839
5	23.782369039697862	25.640890712088666	26.56651671264295	24.01022353557052
6	23.754732594984198	25.840855396688227	26.438098846480525	23.96631316184705
7	23.803554382013626	25.97837496925494	26.182179131716698	24.035891517014736
8	23.933044183619813	26.067988772353264	25.95664391434963	24.042323129677293
9	24.015466274377275	26.174032422344123	25.942085082049836	23.868416221228767
10-14	23.9539566696408	26.15480384945661	25.93396565830674	23.957273822595855
15-19	23.923806049068137	26.123573497546047	26.012926372067625	23.93969408131819
20-24	23.886190859859838	26.006950819212044	26.068456526001448	24.038401794926667
25-29	23.91347648933736	25.987698858642116	25.967067024810042	24.131757627210476
30-34	23.940801098285565	26.036676563554273	25.944973460827388	24.077548877332774
35-39	23.960945688734114	26.017397317354867	25.96066659572403	24.060990398186988
40-44	23.98488687962711	26.012267619013095	25.91348974235739	24.089355759002405
45-49	24.089843002385933	25.96639657791431	25.856404307543347	24.08735611215641
50-54	24.091144916706718	25.993421044938263	25.863939039226207	24.05149499912881
55-59	24.044860693218702	25.951362196072196	25.886118358044367	24.117658752664735
60-64	24.01798434818334	25.988992977068765	25.910792362986186	24.082230311761705
65-69	24.004953511134293	25.977989072495188	25.913283151162776	24.103774265207743
70-74	23.971972980990103	25.99464889826475	25.918334890563187	24.11504323018196
75-79	23.954116485470596	25.98818220407858	25.946018110641667	24.111683199809157
80-84	23.970784107134296	25.99699546239982	25.897360037389106	24.134860393076778
85-89	23.93534397239006	25.964038319938037	25.89577747087941	24.20484023679249
90-94	23.303104601600236	25.85550325750614	27.07507878170713	23.766313359186494
95-99	23.758578847346644	26.13986386546922	26.135898979065164	23.96565830811897
100-104	23.872585911052607	26.190541349391616	26.014534087039543	23.922338652516235
105-109	23.774524628160528	26.128602784369072	26.137038247101437	23.95983434036896
110-114	23.79205709415636	26.03154134816249	26.148657304128903	24.02774425355225
115-119	23.834576356853542	26.08761612932841	25.993056179398156	24.084751334419888
120-124	23.810608196849607	26.091756428050587	26.015134008712277	24.08250136638753
125-129	23.784678478548333	26.097560450523606	26.062257644542562	24.055503426385496
130-134	23.90424741969425	26.069860207651413	25.9440611678284	24.081831204825928
135-139	23.99149234436582	26.039342425571206	25.90557986405865	24.06358536600432
140-144	23.94693923385833	26.132275437923237	25.97505361584736	23.945731712371074
145-149	23.919157933285344	26.15616752118011	25.98261545882805	23.9420590867065
150-154	23.808730963145514	26.22844907422576	26.049112954874833	23.91370700775389
155-159	23.7702137968905	26.282029243341853	26.09972139034284	23.848035569424813
160-164	23.735799258199293	26.375015238295685	26.11655385197119	23.77263165153383
165-169	23.606507595975593	26.411969088164366	26.235357472867943	23.7461658429921
170-174	23.57326436811323	26.512394270127643	26.28119278674169	23.633148575017437
175-178	23.522631406441537	26.57991137057771	26.267055786615607	23.63040143636514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.5
12	3.0
13	11.0
14	32.5
15	65.0
16	131.0
17	273.0
18	562.0
19	1087.5
20	1941.5
21	3355.0
22	5512.0
23	8374.0
24	11875.5
25	16296.5
26	21544.0
27	27647.0
28	34218.0
29	41076.0
30	48843.5
31	56639.0
32	65408.5
33	74517.0
34	83304.0
35	93031.5
36	102610.0
37	112089.5
38	121219.5
39	130455.0
40	139739.5
41	148395.0
42	156213.0
43	163405.0
44	170529.0
45	177213.5
46	182626.5
47	186949.0
48	190535.5
49	192268.5
50	191690.5
51	188234.5
52	182513.5
53	175214.5
54	166306.5
55	156043.5
56	144829.0
57	133449.0
58	122395.5
59	112571.0
60	103855.0
61	95329.0
62	86860.5
63	79079.5
64	71455.0
65	63792.5
66	56249.0
67	48721.0
68	41513.5
69	34482.0
70	28156.5
71	22263.5
72	17023.5
73	12769.5
74	9191.5
75	6366.5
76	4264.0
77	2749.5
78	1741.5
79	1042.0
80	586.0
81	326.5
82	165.5
83	91.5
84	40.0
85	15.5
86	9.0
87	4.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	731.0
92-93	1440.0
94-95	2258.0
96-97	2824.0
98-99	3553.0
100-101	4295.0
102-103	4743.0
104-105	5599.0
106-107	6202.0
108-109	6807.0
110-111	7294.0
112-113	7755.0
114-115	8142.0
116-117	8503.0
118-119	9200.0
120-121	9312.0
122-123	9800.0
124-125	10410.0
126-127	10751.0
128-129	11270.0
130-131	11787.0
132-133	13144.0
134-135	59100.0
136-137	51590.0
138-139	58648.0
140-141	51438.0
142-143	52170.0
144-145	52286.0
146-147	54619.0
148-149	55314.0
150-151	54567.0
152-153	51063.0
154-155	51526.0
156-157	51419.0
158-159	52437.0
160-161	55327.0
162-163	56497.0
164-165	57517.0
166-167	56895.0
168-169	60695.0
170-171	60002.0
172-173	66845.0
174-175	67061.0
176-177	177177.0
178-179	3620893.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.88101108907524
#Duplication Level	Percentage of total
1	94.0944854552493
2	5.126370883727795
3	0.5013663436408845
4	0.13480036957276745
5	0.057147619217422306
6	0.02308127279980997
7	0.01588970777907307
8	0.020321314241679482
9	0.0075588501457551135
>10	0.01897818362552927
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.846920602326373E-5	0.0	0.0	1.948973534108791E-5	0.0	0.0
2	0.001266832797170714	3.897947068217582E-5	0.0	1.948973534108791E-5	0.0	0.0
3	0.001266832797170714	3.897947068217582E-5	0.0	1.948973534108791E-5	1.948973534108791E-5	0.0
4	0.001266832797170714	5.846920602326373E-5	1.948973534108791E-5	1.948973534108791E-5	1.948973534108791E-5	0.0
5	0.001266832797170714	7.795894136435164E-5	3.897947068217582E-5	1.948973534108791E-5	1.948973534108791E-5	0.0
6	0.001266832797170714	7.795894136435164E-5	3.897947068217582E-5	1.948973534108791E-5	1.948973534108791E-5	0.0
7	0.001266832797170714	7.795894136435164E-5	3.897947068217582E-5	5.846920602326373E-5	1.948973534108791E-5	0.0
8	0.001266832797170714	7.795894136435164E-5	3.897947068217582E-5	7.795894136435164E-5	1.948973534108791E-5	0.0
9	0.001266832797170714	7.795894136435164E-5	3.897947068217582E-5	7.795894136435164E-5	1.948973534108791E-5	0.0
10-14	0.001266832797170714	1.0134662377365713E-4	6.236715309148131E-5	1.0914251791009229E-4	1.948973534108791E-5	0.0
15-19	0.001266832797170714	1.5591788272870328E-4	9.355072963722196E-5	1.325302003193978E-4	1.948973534108791E-5	0.0
20-24	0.001266832797170714	1.7540761806979118E-4	1.2083635911474505E-4	1.5981582979692087E-4	2.7285629477523072E-5	0.0
25-29	0.0012707307442389316	1.7540761806979118E-4	1.6761172393335604E-4	1.948973534108791E-4	3.897947068217582E-5	0.0
30-34	0.0012902204795800195	1.987953004790967E-4	1.7540761806979118E-4	2.2218298288840215E-4	7.406099429613405E-5	0.0
35-39	0.001372077368012589	2.2608092995661972E-4	1.8320351220622635E-4	2.9234603011631864E-4	1.1693841204652746E-4	1.1693841204652745E-5
40-44	0.001481219885922681	2.533665594341429E-4	2.0659119461553186E-4	3.196316595938417E-4	1.7540761806979118E-4	3.1183576545740656E-5
45-49	0.0015552808802188152	2.9234603011631864E-4	2.14387088751967E-4	3.703049714806703E-4	2.4946861236592525E-4	7.016304722791647E-5
50-54	0.0016371377686513842	2.9234603011631864E-4	2.14387088751967E-4	4.28774177503934E-4	3.196316595938417E-4	1.0524457084187472E-4
55-59	0.0016839131334699955	2.962439771845362E-4	2.6895834770701316E-4	4.794474893907626E-4	3.3132550079849446E-4	1.1693841204652746E-4
60-64	0.0017540761806979117	3.5471318320779994E-4	2.9234603011631864E-4	5.613043778233317E-4	3.5081523613958237E-4	1.2473430618296263E-4
65-69	0.0017813618101754347	4.0928444216284604E-4	2.962439771845362E-4	6.197735838465955E-4	4.2487623043571644E-4	1.987953004790967E-4
70-74	0.0021282790992467994	4.482639128450219E-4	3.157337125256241E-4	6.665489486652064E-4	4.8724338352719774E-4	2.1828503582018457E-4
75-79	0.008489728714577894	4.677536481861098E-4	3.5081523613958237E-4	7.172222605520351E-4	5.301208012775912E-4	2.338768240930549E-4
80-84	0.01105847583253328	4.677536481861098E-4	3.8589675975354056E-4	7.83487360711734E-4	6.236715309148131E-4	2.338768240930549E-4
85-89	0.011124740932692978	4.950392776636328E-4	4.443659657768043E-4	8.107729901892571E-4	7.600996783024284E-4	2.338768240930549E-4
90-94	0.011257271133012376	5.184269600729384E-4	5.067331188682856E-4	8.497524608714329E-4	7.873853077799515E-4	2.338768240930549E-4
95-99	0.011300148550762772	5.457125895504615E-4	5.067331188682856E-4	8.731401432807383E-4	7.990791489846042E-4	2.338768240930549E-4
100-104	0.011327434180240294	5.846920602326373E-4	5.184269600729384E-4	8.965278256900438E-4	7.990791489846042E-4	2.338768240930549E-4
105-109	0.011358617756786033	6.002838485055076E-4	5.262228542093735E-4	9.31609349304002E-4	7.990791489846042E-4	2.338768240930549E-4
110-114	0.011389801333331773	6.236715309148131E-4	5.535084836868966E-4	0.001005670343600136	7.990791489846042E-4	2.338768240930549E-4
115-119	0.011432678751082167	6.392633191876834E-4	5.652023248915493E-4	0.0010563436554869646	7.990791489846042E-4	2.338768240930549E-4
120-124	0.011522331533651172	6.509571603923361E-4	5.691002719597669E-4	0.0011070169673737932	8.224668313939098E-4	2.338768240930549E-4
125-129	0.01173671862240314	6.899366310745119E-4	6.470592133241185E-4	0.00119277180287458	8.692421962125208E-4	2.338768240930549E-4
130-134	0.011923820081677583	7.016304722791647E-4	6.626510015969889E-4	0.0012278533264885383	9.705888199861779E-4	2.338768240930549E-4
135-139	0.012107023593883808	7.17222260552035E-4	6.821407369380767E-4	0.0012278533264885383	0.001025160078941224	2.533665594341428E-4
140-144	0.012247349688339642	7.484058370977756E-4	7.016304722791647E-4	0.0012473430618296262	0.0010758333908280526	2.572645065023604E-4
145-149	0.012352594259181516	7.63997625370646E-4	7.055284193473823E-4	0.0012941184266482373	0.0011693841204652746	2.7285629477523073E-4
150-154	0.012403267571068344	7.795894136435164E-4	7.211202076202526E-4	0.001360383526807936	0.0012200574323521032	2.7285629477523073E-4
155-159	0.012485124459500913	7.83487360711734E-4	7.328140488249054E-4	0.0013876691562854592	0.0012551389559660613	2.845501359798835E-4
160-164	0.012629348501024965	8.263647784621273E-4	7.484058370977756E-4	0.0014227506798994173	0.0013019143207846722	2.9234603011631864E-4
165-167	0.01277227322685961	8.575483550078679E-4	7.795894136435164E-4	0.001435743836793476	0.001351288316982095	2.9234603011631864E-4
>>END_MODULE
