##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293865
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7437948
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.39413370461853	39.0	39.0	39.0	37.0	39.0
2	40.18239022375526	41.0	40.0	41.0	38.0	41.0
3	40.219679271756135	41.0	40.0	41.0	38.0	41.0
4	40.22734455793453	41.0	40.0	41.0	38.0	41.0
5	40.22023695244979	41.0	40.0	41.0	38.0	41.0
6	40.21031176878354	41.0	40.0	41.0	38.0	41.0
7	40.23336288449449	41.0	40.0	41.0	38.0	41.0
8	40.22229840810933	41.0	40.0	41.0	38.0	41.0
9	40.207466763682675	41.0	40.0	41.0	38.0	41.0
10-14	40.147362041251164	41.0	40.0	41.0	38.0	41.0
15-19	40.023525628305016	41.0	40.0	41.0	38.0	41.0
20-24	39.835647224207534	41.0	40.0	41.0	37.6	41.0
25-29	39.5415169613985	40.6	39.0	41.0	36.4	41.0
30-34	39.230831245391876	40.0	38.4	41.0	35.0	41.0
35-39	38.97975409346772	40.0	37.6	41.0	35.0	41.0
40-44	38.42197641069822	39.6	35.8	41.0	35.0	41.0
45-49	37.71526052615587	38.2	35.0	41.0	35.0	41.0
50-54	37.02145658990894	36.6	35.0	39.8	34.0	41.0
55-59	36.40068578054055	35.2	35.0	38.8	34.0	41.0
60-64	35.74284711320918	35.0	34.8	36.8	33.6	39.8
65-69	35.4692776825006	35.0	35.0	36.0	33.6	39.0
70-74	35.301848708810546	35.0	35.0	35.8	34.0	37.8
75-79	35.14579050566097	35.0	35.0	35.0	33.6	37.0
80-84	35.07953793169837	35.0	35.0	35.0	33.0	37.0
85-89	34.88862820767233	35.0	34.6	35.0	32.6	37.2
90-94	39.601562342594725	40.6	39.8	40.6	37.8	40.6
95-99	39.87492285289704	41.0	40.0	41.0	37.6	41.0
100-104	39.755847162799235	41.0	40.0	41.0	36.8	41.0
105-109	39.594476196547994	41.0	39.4	41.0	36.0	41.0
110-114	39.389286419073684	40.8	39.0	41.0	35.2	41.0
115-119	39.068023331571695	40.0	38.0	41.0	35.0	41.0
120-124	38.73237530661452	40.0	37.2	41.0	35.0	41.0
125-129	38.1324703042634	39.6	35.8	40.6	34.4	41.0
130-134	37.7750120468086	38.8	35.0	41.0	34.0	41.0
135-139	37.376841124661404	37.2	35.0	40.8	34.0	41.0
140-144	36.80848561897867	36.0	35.0	39.4	34.0	41.0
145-149	36.19287505832777	35.0	35.0	38.0	34.0	40.8
150-154	35.6482672030701	35.0	35.0	36.6	34.0	39.2
155-159	35.216783350367606	35.0	35.0	35.8	33.0	37.4
160-164	34.90483734996572	35.0	35.0	35.0	33.0	36.4
165-169	34.704500839154754	35.0	35.0	35.0	33.0	36.0
170-174	34.58210330428314	35.0	35.0	35.0	33.0	35.8
175-178	34.275104196851615	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	28.0
30	383.0
31	3200.0
32	16567.0
33	62957.0
34	182325.0
35	468317.0
36	1302108.0
37	2492202.0
38	2205754.0
39	688617.0
40	15487.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.29962511165714	25.878710095849016	26.82735883606608	23.994305956427766
2	24.15728101352685	26.29468503947594	26.617879017169788	22.93015492982742
3	22.37168100664323	25.76691851032032	27.68887332904183	24.172527153994626
4	23.054611298707655	25.041032822493513	27.940958984924336	23.963396893874496
5	23.880766577018285	25.544424349296342	26.481537649900215	24.09327142378516
6	23.83358958680539	25.767873074670593	26.34184858512052	24.056688753403492
7	23.885633510747855	25.931385914502226	26.08626734147644	24.09671323327348
8	24.01199900832864	26.03926513065163	25.876169072437722	24.072566788582012
9	24.087880151891355	26.147346015325734	25.8603851492374	23.904388683545516
10-14	24.02316606677003	26.077929020208263	25.86418727315652	24.03471763986519
15-19	23.9863494608997	26.04016053890132	25.965099514005747	24.008390486193235
20-24	23.958688606051023	25.935612886780063	26.02465357380826	24.08104493336065
25-29	23.97608049962167	25.931423559293503	25.895338337939442	24.197157603145385
30-34	24.019745768591015	25.9693829534705	25.88835791807095	24.122513359867533
35-39	24.047175376864693	25.926986851749973	25.888901078630827	24.136936692754507
40-44	24.037516798988108	25.940665355552365	25.86195547481644	24.159862370643086
45-49	24.175213378743706	25.886284765636976	25.797894795715166	24.140607059904156
50-54	24.139047489979763	25.9231013715073	25.81002986307514	24.127821275437793
55-59	24.11323929664472	25.87554524446796	25.81922325888807	24.191992199999245
60-64	24.0936586273526	25.918629708086154	25.84452862536818	24.143183039193065
65-69	24.08077873090804	25.90407730734337	25.851691891365736	24.163452070382853
70-74	24.031915791828606	25.913674040205713	25.850245255815178	24.204164912150503
75-79	24.032510041748072	25.90364439224367	25.88727966369219	24.176565902316067
80-84	24.023585537301418	25.921243332166345	25.87034488544421	24.184826245088026
85-89	24.011388624927196	25.887865846870667	25.85190969337242	24.248835834829713
90-94	23.371264519686953	25.772752438908846	27.011643449842417	23.844339591561784
95-99	23.859178710385873	26.046781244242723	26.052849646660942	24.041190398710462
100-104	23.954918519706453	26.105455284074598	25.931628248407733	24.007997947811216
105-109	23.883156438409532	26.05029637160167	26.033460824877974	24.03308636511082
110-114	23.891971860807068	25.944465695247636	26.056012739519595	24.107549704425704
115-119	23.93332524923326	25.9859064160959	25.91569736944292	24.165070965227926
120-124	23.889930272172958	25.99850624489124	25.9552338332329	24.1563296497029
125-129	23.876963677942218	26.025450151764474	25.97409002344506	24.123496146848243
130-134	23.98524392745019	26.005611370650765	25.86445802556919	24.14468667632986
135-139	24.055128962394175	25.96524447775329	25.84112386303572	24.13850269681682
140-144	24.040571394086836	26.023582828226676	25.91172292011054	24.024122857575943
145-149	23.997891718251115	26.04447573036041	25.9192260754185	24.03840647596997
150-154	23.920194707560647	26.10810087845087	25.979109639602683	23.992594774385804
155-159	23.884723590747882	26.148778423532455	26.019995813948384	23.94650217177128
160-164	23.850927402241364	26.25400951438472	26.024049004712353	23.871014078661563
165-169	23.740999880737277	26.274459166454236	26.10845744359097	23.876083509217512
170-174	23.701148780366548	26.371901813043095	26.166251265183732	23.76069814140662
175-178	23.654662250451644	26.3999353430946	26.16491551934031	23.78048688711345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	4.5
12	11.5
13	20.5
14	40.5
15	88.0
16	189.0
17	383.0
18	777.5
19	1520.0
20	2775.0
21	4839.5
22	7827.0
23	11760.5
24	16904.5
25	23225.5
26	30617.0
27	39017.5
28	48570.0
29	58910.0
30	69501.5
31	80961.5
32	93562.5
33	106192.0
34	118740.0
35	132134.5
36	146105.0
37	160081.0
38	173882.5
39	186887.0
40	199978.0
41	212664.0
42	224519.5
43	235737.5
44	246122.5
45	256001.5
46	264112.0
47	271056.0
48	275845.0
49	277899.0
50	277534.5
51	273152.5
52	265200.5
53	254630.5
54	241204.5
55	226184.5
56	210612.0
57	195044.0
58	179634.0
59	165012.5
60	151866.0
61	139845.5
62	128421.0
63	117209.5
64	106207.5
65	94998.5
66	83843.5
67	72818.5
68	62118.5
69	51868.0
70	42255.0
71	33619.5
72	26030.5
73	19330.5
74	13786.5
75	9723.0
76	6625.5
77	4260.5
78	2663.5
79	1585.5
80	922.0
81	513.0
82	262.0
83	137.5
84	68.5
85	28.0
86	14.0
87	7.5
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	894.0
92-93	2090.0
94-95	3985.0
96-97	4779.0
98-99	4878.0
100-101	5767.0
102-103	6778.0
104-105	7774.0
106-107	9092.0
108-109	9996.0
110-111	10908.0
112-113	11967.0
114-115	12931.0
116-117	13174.0
118-119	13013.0
120-121	14812.0
122-123	14173.0
124-125	15952.0
126-127	15796.0
128-129	16071.0
130-131	16628.0
132-133	18748.0
134-135	83807.0
136-137	76660.0
138-139	76310.0
140-141	75220.0
142-143	71322.0
144-145	76366.0
146-147	77404.0
148-149	85082.0
150-151	79915.0
152-153	72616.0
154-155	75083.0
156-157	71063.0
158-159	73713.0
160-161	77283.0
162-163	74184.0
164-165	76892.0
166-167	79694.0
168-169	95510.0
170-171	83867.0
172-173	90274.0
174-175	90562.0
176-177	246053.0
178-179	5298862.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.76018713167764
#Duplication Level	Percentage of total
1	92.10038162721186
2	6.583658806841821
3	0.8062389569945498
4	0.2171952648696407
5	0.08828411965895616
6	0.07130828193905821
7	0.03745359232636394
8	0.032467296130102505
9	0.020032024119089276
>10	0.042980029908553374
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.72228415686692E-5	1.3444568313733842E-5	0.0	0.0	0.0	0.0
2	0.0012503448531772472	1.3444568313733842E-5	1.3444568313733842E-5	0.0	0.0	0.0
3	0.0012503448531772472	1.3444568313733842E-5	1.3444568313733842E-5	0.0	0.0	0.0
4	0.0012503448531772472	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5	1.3444568313733842E-5	0.0
5	0.0012503448531772472	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5	1.3444568313733842E-5	1.3444568313733842E-5
6	0.0012503448531772472	4.0333704941201524E-5	1.3444568313733842E-5	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5
7	0.0012503448531772472	4.0333704941201524E-5	1.3444568313733842E-5	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5
8	0.0012637894214909812	5.377827325493537E-5	2.6889136627467684E-5	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5
9	0.0012637894214909812	8.066740988240305E-5	2.6889136627467684E-5	2.6889136627467684E-5	1.3444568313733842E-5	1.3444568313733842E-5
10-14	0.0012637894214909812	9.142306453339013E-5	4.5711532266695064E-5	3.226696395296122E-5	1.344456831373384E-5	1.344456831373384E-5
15-19	0.0012772339898047152	1.2100111482360458E-4	6.991175523141598E-5	9.411197819613689E-5	1.344456831373384E-5	1.344456831373384E-5
20-24	0.0012933674717811954	1.425124241255787E-4	1.183122011608578E-4	1.1293437383536426E-4	1.344456831373384E-5	1.344456831373384E-5
25-29	0.0013068120400949294	1.5595699243931257E-4	1.3982351046283194E-4	1.640237334275529E-4	3.226696395296122E-5	1.344456831373384E-5
30-34	0.0013337011767223972	1.801572154040335E-4	1.5595699243931257E-4	2.2586874767072852E-4	5.377827325493536E-5	1.613348197648061E-5
35-39	0.0013874794499773326	1.9091287005502055E-4	1.9091287005502057E-4	2.5006897063544945E-4	9.411197819613689E-5	4.571153226669506E-5
40-44	0.0014681468598597355	2.0704635203150118E-4	2.3124657499622208E-4	3.2266963952961214E-4	1.6671264709029965E-4	1.0755654650987072E-4
45-49	0.0015407475287538982	2.393133159844624E-4	2.635135389491833E-4	4.2753727237673615E-4	2.366244023217156E-4	1.5864590610205933E-4
50-54	0.0016267927659617948	2.8502484825115745E-4	3.1998072586686546E-4	4.571153226669506E-4	3.0384724389038477E-4	1.9360178371776734E-4
55-59	0.0016993934348559577	3.4149203516883956E-4	3.6569225813356046E-4	5.055157685963925E-4	3.468698624943331E-4	2.1511309301974144E-4
60-64	0.0017289714851461719	3.6031443080806693E-4	4.3291509970222973E-4	5.56605128188581E-4	3.576255171453202E-4	2.2317983400798176E-4
65-69	0.001788127585726601	3.818257401100411E-4	4.813155456316716E-4	6.345836244082373E-4	4.0871487673750884E-4	2.527578842981962E-4
70-74	0.0021833978941503763	4.2753727237673615E-4	4.974490276081522E-4	7.044953796396533E-4	5.055157685963925E-4	2.662024526119301E-4
75-79	0.008214631239691378	4.3829292702772326E-4	5.055157685963924E-4	7.233177752788807E-4	5.404716462121005E-4	2.930915892393977E-4
80-84	0.010739521169010594	4.893822866199118E-4	5.270270778983666E-4	7.528958255690951E-4	6.776062430121855E-4	3.0922507121587835E-4
85-89	0.010790610528602782	5.619829555140746E-4	5.539162145258343E-4	7.986073578357901E-4	9.545643502751027E-4	3.0922507121587835E-4
90-94	0.010906233816100893	5.834942648160487E-4	6.076944877807698E-4	8.523856310907257E-4	9.545643502751027E-4	3.0922507121587835E-4
95-99	0.010930434039065613	5.969388331297825E-4	6.292057970827439E-4	8.738969403926997E-4	9.545643502751027E-4	3.0922507121587835E-4
100-104	0.010930434039065613	6.318947107454906E-4	6.318947107454906E-4	8.819636813809401E-4	9.545643502751027E-4	3.0922507121587835E-4
105-109	0.010941189693716599	6.560949337102115E-4	6.480281927219712E-4	9.088528180084077E-4	9.599421776005963E-4	3.0922507121587835E-4
110-114	0.010946567521042094	6.722284156866921E-4	6.695395020239453E-4	9.438086956241158E-4	9.706978322515835E-4	3.0922507121587835E-4
115-119	0.010957323175693081	6.991175523141599E-4	7.044953796396533E-4	9.948980552163043E-4	9.814534869025705E-4	3.0922507121587835E-4
120-124	0.010970767744006816	7.502069119063484E-4	7.367623435926146E-4	0.00105674306945948	9.948980552163043E-4	3.0922507121587835E-4
125-129	0.011019168189936257	7.851627895220564E-4	7.528958255690951E-4	0.0011078324290516685	0.001021787191843772	3.226696395296122E-4
130-134	0.011148236045748102	7.932295305102966E-4	7.824738758593096E-4	0.0011642996159693508	0.0010755654650987073	3.226696395296122E-4
135-139	0.011323015433826644	8.093630124867774E-4	8.066740988240305E-4	0.0012180778892242861	0.0011320326520163893	3.226696395296122E-4
140-144	0.011470905685277713	8.362521491142449E-4	8.254964944632579E-4	0.0012342113712007668	0.0011884998389340717	3.226696395296122E-4
145-149	0.011538128526846382	8.658301994044594E-4	8.47007803765232E-4	0.0013202566084086632	0.0012342113712007666	3.226696395296122E-4
150-154	0.01155695092248561	8.873415087064336E-4	8.604523720789658E-4	0.0013444568313733841	0.001263789421490981	3.226696395296122E-4
155-159	0.011637618332368014	9.034749906829141E-4	8.738969403926997E-4	0.001371345968000852	0.0013202566084086632	3.307363805178525E-4
160-164	0.011699463346611188	9.303641273103819E-4	8.954082496946738E-4	0.0014197464139302936	0.0013659681406753585	3.3611420784334604E-4
165-167	0.011772960320059601	9.545643502751026E-4	9.187121681051459E-4	0.0014340872867982767	0.0013982351046283195	3.3611420784334604E-4
>>END_MODULE
