##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293866
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9099281
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.31715560822882	39.0	39.0	39.0	37.0	39.0
2	40.047731353719044	41.0	40.0	41.0	38.0	41.0
3	40.07951353519031	41.0	40.0	41.0	38.0	41.0
4	40.08726524656179	41.0	40.0	41.0	38.0	41.0
5	40.083771234232685	41.0	40.0	41.0	38.0	41.0
6	40.07586423586655	41.0	40.0	41.0	38.0	41.0
7	40.094941017867235	41.0	40.0	41.0	38.0	41.0
8	40.07010663809591	41.0	40.0	41.0	38.0	41.0
9	40.04782894384732	41.0	40.0	41.0	38.0	41.0
10-14	39.987835632287876	41.0	40.0	41.0	38.0	41.0
15-19	39.858578540436326	41.0	40.0	41.0	38.0	41.0
20-24	39.686947045596234	40.2	39.6	41.0	37.4	41.0
25-29	39.56563038332369	40.0	39.0	41.0	37.0	41.0
30-34	39.41015528589567	40.0	39.0	41.0	36.2	41.0
35-39	39.31953333455688	40.0	38.8	41.0	35.4	41.0
40-44	38.98104105148528	40.0	37.6	41.0	35.0	41.0
45-49	38.37304694733573	39.4	36.4	41.0	35.0	41.0
50-54	37.640236739584154	38.0	35.0	40.4	34.2	41.0
55-59	36.86470704663368	36.6	35.0	39.2	34.0	41.0
60-64	35.903663707055536	35.4	34.8	37.4	33.4	40.0
65-69	35.44287571732316	35.0	34.8	36.6	33.0	39.0
70-74	35.20637061323856	35.0	35.0	36.0	33.0	37.2
75-79	35.00083032934141	35.0	35.0	35.2	33.0	36.8
80-84	34.92125632783514	35.0	35.0	35.0	33.0	36.0
85-89	34.71895968483663	35.0	34.6	35.0	32.4	36.0
90-94	39.42436535255856	40.6	39.6	40.6	36.8	40.6
95-99	39.71751571238493	41.0	40.0	41.0	37.0	41.0
100-104	39.6123320387582	41.0	39.0	41.0	37.0	41.0
105-109	39.45240739108074	40.0	39.0	41.0	36.0	41.0
110-114	39.260893607532665	40.0	38.4	41.0	35.4	41.0
115-119	39.01811936364107	40.0	38.0	41.0	35.0	41.0
120-124	38.70089822622978	40.0	37.8	41.0	34.8	41.0
125-129	38.14505426370725	39.4	36.8	40.4	34.4	40.6
130-134	38.13156372495659	39.0	36.0	40.8	34.0	41.0
135-139	38.012424559111324	39.0	35.4	41.0	34.0	41.0
140-144	37.341187443829156	37.4	35.0	40.2	34.0	41.0
145-149	36.57714503064075	36.2	35.0	39.0	34.0	40.8
150-154	35.84579028060591	35.0	35.0	37.0	33.6	39.2
155-159	35.25196975411184	35.0	35.0	36.0	33.0	37.8
160-164	34.848142558635296	35.0	35.0	35.4	33.0	36.6
165-169	34.61865516408703	35.0	35.0	35.0	33.0	36.0
170-174	34.49601065756997	35.0	34.0	35.0	33.0	36.0
175-178	34.20617493607014	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	112.0
30	954.0
31	5927.0
32	26869.0
33	88763.0
34	231106.0
35	529312.0
36	1256487.0
37	2858467.0
38	3407459.0
39	691375.0
40	2447.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.159034433599754	28.054326490191915	28.988587120235103	21.798051955973225
2	21.799085004628388	28.744556850151127	29.017864158717593	20.43849398650289
3	19.791739589095005	27.65772372564382	30.736604353684648	21.813932331576527
4	20.74452915565527	26.71911110339377	30.77761858327048	21.75874115768048
5	21.604816908061196	27.670746732626455	28.880710464925745	21.8437258943866
6	21.706330423249927	27.823912680573333	28.659308356341562	21.810448539835182
7	21.82037240085233	28.12449686958783	28.12695860255332	21.92817212700652
8	21.845176558455552	28.42220171022304	28.044061942916148	21.68855978840526
9	21.939381803902968	28.295235634551787	28.057293757605684	21.708088803939564
10-14	21.909733307499792	28.317465962420545	27.950511694275626	21.822289035804037
15-19	21.88595780260001	28.254021389162503	28.087973104688164	21.772047703549326
20-24	21.7933504856043	28.174997562994264	28.20477354199744	21.826878409403996
25-29	21.835241707559092	28.162675710311618	28.052009823633316	21.950072758495974
30-34	21.858087468669225	28.191550519211354	28.069888159295225	21.880473852824196
35-39	21.81139366945586	28.22108252289384	28.115975317170665	21.851548490479633
40-44	21.838411188752165	28.220271469800746	28.06141716032289	21.8799001811242
45-49	21.97872996778537	28.0908260773571	28.022647064092205	21.907796890765326
50-54	21.925712592016886	28.206940746197418	28.032801712574873	21.834544949210823
55-59	21.906190170410166	28.15789511281166	28.00071346296482	21.93520125381335
60-64	21.89915444967575	28.158411637139242	28.041905728595477	21.90052818458953
65-69	21.851012184369292	28.178481354735613	28.05721682845051	21.913289632444584
70-74	21.819677840479923	28.213987456811147	28.016659777843984	21.949674924864944
75-79	21.828177413138466	28.15122425607034	28.06444377308493	21.956154557706263
80-84	21.78312550189405	28.229623857093767	28.04127490952307	21.945975731489114
85-89	21.715302560718808	28.261553852441747	27.992233672089036	22.030909914750406
90-94	21.027060949005026	27.83613791279355	29.537953214983332	21.598847923218095
95-99	21.696634518886313	28.250658708169073	28.205776885821894	21.846929887122723
100-104	21.773046055034253	28.344468634412074	28.058077246962853	21.824408063590813
105-109	21.69035848729827	28.347548984917225	28.151607991574135	21.810484536210367
110-114	21.67577061140052	28.16660493300363	28.232443353462283	21.925181102133564
115-119	21.687292217101266	28.256633694633564	28.11127377909116	21.944800309174003
120-124	21.64816009189914	28.288899014337492	28.09016480237192	21.972776091391445
125-129	21.635911870696482	28.2450547369683	28.173128357396916	21.945905034938306
130-134	21.752828945639557	28.263164199686443	28.061753300568647	21.922253554105353
135-139	21.841701252033495	28.208138901400183	28.00491628742922	21.9452435591371
140-144	21.83875521647506	28.23129762540766	28.065521700540952	21.86442545757633
145-149	21.774652009907197	28.28954130811824	28.08460073091039	21.851205951064173
150-154	21.729032991643493	28.360065410409053	28.075756550924506	21.835145047022948
155-159	21.69579006884672	28.339184761022196	28.144934108447707	21.820091061683385
160-164	21.63871402673363	28.469108892701534	28.16090383975632	21.73127324080852
165-169	21.55696396558864	28.468347099130654	28.194932402586765	21.779756532693945
170-174	21.53574373512959	28.527583378595704	28.241446435209642	21.695226451065057
175-178	21.470489457554244	28.64125341147265	28.186856892940572	21.701400238032537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	1.5
12	3.5
13	9.5
14	19.0
15	51.0
16	112.0
17	231.0
18	474.5
19	884.0
20	1721.5
21	3192.0
22	5574.0
23	9485.5
24	15344.5
25	23630.5
26	34726.0
27	49081.0
28	67018.0
29	87980.5
30	112010.5
31	139119.0
32	168271.5
33	199349.0
34	232411.5
35	265563.5
36	298220.0
37	330036.5
38	359489.0
39	386413.5
40	410270.0
41	428575.5
42	440634.0
43	445973.0
44	442359.0
45	431731.0
46	414749.0
47	390768.5
48	361660.0
49	328637.5
50	294477.5
51	260996.5
52	229044.5
53	199328.5
54	172407.0
55	149133.0
56	129097.5
57	112104.0
58	98225.0
59	86940.0
60	76841.0
61	67818.5
62	59901.5
63	52460.0
64	45235.0
65	38502.0
66	32486.5
67	26650.0
68	21356.5
69	16887.0
70	12945.0
71	9612.0
72	6984.0
73	4979.5
74	3476.5
75	2345.0
76	1542.0
77	975.0
78	582.5
79	350.5
80	205.5
81	114.0
82	49.5
83	23.5
84	14.0
85	6.5
86	2.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	863.0
92-93	1942.0
94-95	2972.0
96-97	3926.0
98-99	4866.0
100-101	5756.0
102-103	6754.0
104-105	7425.0
106-107	8307.0
108-109	9455.0
110-111	10198.0
112-113	10806.0
114-115	11569.0
116-117	12172.0
118-119	13186.0
120-121	13594.0
122-123	14004.0
124-125	15024.0
126-127	15655.0
128-129	16326.0
130-131	17103.0
132-133	19027.0
134-135	88094.0
136-137	85708.0
138-139	98557.0
140-141	81997.0
142-143	87130.0
144-145	90591.0
146-147	91549.0
148-149	84893.0
150-151	85295.0
152-153	86159.0
154-155	91144.0
156-157	84716.0
158-159	88174.0
160-161	98854.0
162-163	94255.0
164-165	99057.0
166-167	99676.0
168-169	111100.0
170-171	101719.0
172-173	104159.0
174-175	108101.0
176-177	292867.0
178-179	6624556.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.68132028144552
#Duplication Level	Percentage of total
1	84.98984383835986
2	11.316606030191572
3	2.236652728912191
4	0.6802823487600924
5	0.2715696099039687
6	0.16527459473423267
7	0.0784458677749996
8	0.0788754568443181
9	0.03965329402060835
>10	0.1322278951750015
>50	0.004255972431384889
>100	0.006312362891789504
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.395951724097761E-5	0.0	0.0	0.0	0.0	0.0
2	3.1870649999708766E-4	0.0	2.1979758620488806E-5	2.1979758620488806E-5	0.0	0.0
3	3.1870649999708766E-4	1.0989879310244403E-5	3.296963793073321E-5	4.395951724097761E-5	0.0	0.0
4	3.1870649999708766E-4	1.0989879310244403E-5	4.395951724097761E-5	5.4949396551222013E-5	0.0	0.0
5	3.1870649999708766E-4	1.0989879310244403E-5	4.395951724097761E-5	7.692915517171082E-5	0.0	0.0
6	3.1870649999708766E-4	2.1979758620488806E-5	4.395951724097761E-5	7.692915517171082E-5	0.0	0.0
7	3.1870649999708766E-4	2.1979758620488806E-5	4.395951724097761E-5	7.692915517171082E-5	0.0	0.0
8	3.406862586175765E-4	2.1979758620488806E-5	4.395951724097761E-5	7.692915517171082E-5	1.0989879310244403E-5	0.0
9	3.406862586175765E-4	2.1979758620488806E-5	4.395951724097761E-5	8.791903448195522E-5	1.0989879310244403E-5	0.0
10-14	3.49478162065772E-4	3.296963793073321E-5	4.835546896507538E-5	1.3187855172293282E-4	2.1979758620488806E-5	0.0
15-19	3.538741137898698E-4	4.395951724097761E-5	7.692915517171082E-5	1.9122389999825262E-4	2.8573686206635448E-5	0.0
20-24	3.626660172380653E-4	5.4949396551222013E-5	1.1869069655063955E-4	2.39579368963328E-4	4.395951724097761E-5	0.0
25-29	3.626660172380653E-4	7.033522758556417E-5	1.3187855172293284E-4	2.527672241356213E-4	4.395951724097761E-5	0.0
30-34	3.714579206862608E-4	1.0110688965424852E-4	1.3847247930907949E-4	2.901328137904522E-4	4.395951724097761E-5	0.0
35-39	4.593769551682161E-4	1.0989879310244403E-4	1.5605628620547052E-4	3.0771662068684326E-4	6.154332413736866E-5	2.1979758620488806E-5
40-44	5.538899172363179E-4	1.1649272068859067E-4	2.2199556206693692E-4	3.1870649999708766E-4	8.352308275785746E-5	5.055344482712426E-5
45-49	6.26423120683931E-4	1.4286843103317724E-4	2.615591275838168E-4	3.428842344796254E-4	9.451296206810185E-5	5.934534827531977E-5
50-54	7.692915517171082E-4	1.6484818965366605E-4	2.9892471723864776E-4	3.538741137898698E-4	1.1649272068859067E-4	8.132510689580857E-5
55-59	8.594085620611123E-4	1.714421172398127E-4	3.143105482729899E-4	3.956356551687985E-4	1.5825426206751938E-4	9.451296206810185E-5
60-64	9.055660551641387E-4	1.824319965500571E-4	3.3409233103142983E-4	4.1102148620314065E-4	1.9781782758439924E-4	1.1429474482654179E-4
65-69	9.715053310256052E-4	1.9781782758439924E-4	3.626660172380653E-4	4.505850517200205E-4	2.39579368963328E-4	1.3847247930907949E-4
70-74	0.001635294041364367	2.066097310325948E-4	3.824477999965052E-4	5.560878930983668E-4	2.57163175859719E-4	1.6045223792956827E-4
75-79	0.012231735672302022	2.2639151379103467E-4	4.41793148271825E-4	6.154332413736864E-4	2.879348379284033E-4	1.8682794827415484E-4
80-84	0.016799129513639592	2.549651999976702E-4	4.505850517200205E-4	6.835704930972018E-4	3.8904172758265186E-4	2.0880770689464366E-4
85-89	0.016860672837776963	2.9452876551455E-4	4.615749310302649E-4	7.055502517176907E-4	5.429000379260735E-4	2.0880770689464366E-4
90-94	0.0168892465239836	3.0991459654889215E-4	4.769607620646071E-4	7.451138172345705E-4	5.604838448224646E-4	2.0880770689464366E-4
95-99	0.01691562223432818	3.2310245172118544E-4	4.945445689609981E-4	7.736875034412059E-4	5.604838448224646E-4	2.0880770689464366E-4
100-104	0.016941997944672774	3.296963793073321E-4	5.231182551676337E-4	8.462207068888191E-4	5.71473724132709E-4	2.0880770689464366E-4
105-109	0.01696177972743121	3.428842344796254E-4	5.385040862019758E-4	8.989721275779921E-4	5.71473724132709E-4	2.0880770689464366E-4
110-114	0.017003541268810138	3.692599448242119E-4	5.494939655122202E-4	9.165559344743831E-4	5.71473724132709E-4	2.0880770689464366E-4
115-119	0.01703870888260292	3.846457758585541E-4	5.582858689604157E-4	9.517235482671653E-4	5.736716999947578E-4	2.0880770689464366E-4
120-124	0.017124429941222825	3.978336310308474E-4	5.956514586152467E-4	9.868911620599474E-4	5.824636034429533E-4	2.1979758620488805E-4
125-129	0.0172760902757042	4.066255344790429E-4	6.176312172357354E-4	0.0010220587758527295	6.000474103393444E-4	2.1979758620488805E-4
130-134	0.01742115668259943	4.132194620651895E-4	6.396109758562243E-4	0.0010748101965419026	6.24225144821882E-4	2.1979758620488805E-4
135-139	0.01757941094466695	4.220113655133851E-4	6.593927586146642E-4	0.0011297595930931247	6.330170482700776E-4	2.3078746551513248E-4
140-144	0.017722279375700124	4.395951724097761E-4	6.835704930972018E-4	0.0011825110137822976	6.527988310285175E-4	2.4397532068742576E-4
145-149	0.017821188289492324	4.461890999959228E-4	7.099462034417884E-4	0.0012198766034371288	6.593927586146642E-4	2.549651999976702E-4
150-154	0.01792669113087067	4.5278302758206934E-4	7.143421551658862E-4	0.0012418563620576176	6.835704930972018E-4	2.637571034458657E-4
155-159	0.0180585696825936	4.659708827543627E-4	7.275300103381794E-4	0.0012902118310226928	7.165401310279351E-4	2.637571034458657E-4
160-164	0.018170666451558095	4.879506413748515E-4	7.473117930966194E-4	0.0013803288413666971	7.473117930966193E-4	2.637571034458657E-4
165-167	0.018353098448108156	5.238509137883166E-4	7.473117930966194E-4	0.0014250210172283573	7.619649655102786E-4	2.674203965492805E-4
>>END_MODULE
