##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293868
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8183046
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.28065453866445	39.0	39.0	39.0	37.0	39.0
2	40.03808789538761	41.0	40.0	41.0	38.0	41.0
3	40.07721207970724	41.0	40.0	41.0	38.0	41.0
4	40.08891346816332	41.0	40.0	41.0	38.0	41.0
5	40.09139726209531	41.0	40.0	41.0	38.0	41.0
6	40.08520995727019	41.0	40.0	41.0	38.0	41.0
7	40.11136879836677	41.0	40.0	41.0	38.0	41.0
8	40.08676145777501	41.0	40.0	41.0	38.0	41.0
9	40.07032063610543	41.0	40.0	41.0	38.0	41.0
10-14	39.99267167751471	41.0	40.0	41.0	38.0	41.0
15-19	39.86777002108995	41.0	40.0	41.0	38.0	41.0
20-24	39.70368034103682	40.4	39.6	41.0	37.4	41.0
25-29	39.58163172979842	40.0	39.0	41.0	37.0	41.0
30-34	39.4241244886073	40.0	39.0	41.0	36.2	41.0
35-39	39.33146578914502	40.0	38.8	41.0	35.6	41.0
40-44	38.983160207091586	40.0	37.6	41.0	35.0	41.0
45-49	38.37785399226645	39.4	36.4	41.0	35.0	41.0
50-54	37.66219845275219	38.2	35.0	40.4	34.4	41.0
55-59	36.87130794572094	36.6	35.0	39.2	34.0	41.0
60-64	35.92825590861887	35.4	34.8	37.4	33.4	40.0
65-69	35.47321672149955	35.0	34.8	36.6	33.4	39.0
70-74	35.2289804554441	35.0	35.0	36.0	33.2	37.2
75-79	35.03302997441294	35.0	35.0	35.2	33.0	36.8
80-84	34.950847300626194	35.0	35.0	35.0	33.0	36.0
85-89	34.78827986546819	35.0	34.6	35.0	32.4	36.6
90-94	39.427626531339016	40.6	39.8	40.6	36.8	40.6
95-99	39.73032969249825	41.0	40.0	41.0	37.0	41.0
100-104	39.615668142436014	41.0	39.0	41.0	37.0	41.0
105-109	39.46180454188142	40.0	39.0	41.0	36.0	41.0
110-114	39.26673931443357	40.0	38.6	41.0	35.6	41.0
115-119	39.01904660472623	40.0	38.0	41.0	35.0	41.0
120-124	38.68059466770005	40.0	37.8	41.0	35.0	41.0
125-129	38.177824136134916	39.4	36.6	40.4	34.4	40.6
130-134	38.13410150129179	39.0	36.0	40.8	34.0	41.0
135-139	38.00337378774991	39.0	35.4	41.0	34.0	41.0
140-144	37.327704238334434	37.2	35.0	40.2	34.0	41.0
145-149	36.568963420270606	36.2	35.0	39.0	34.0	40.8
150-154	35.8527707531007	35.0	35.0	37.0	33.8	39.2
155-159	35.25407494972855	35.0	35.0	36.0	33.0	37.6
160-164	34.870276714301745	35.0	35.0	35.4	33.0	36.4
165-169	34.643010929688046	35.0	35.0	35.0	33.0	36.0
170-174	34.52129829254694	35.0	34.6	35.0	33.0	36.0
175-178	34.23656159164645	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	63.0
30	712.0
31	4649.0
32	21761.0
33	75271.0
34	200246.0
35	464214.0
36	1113707.0
37	2567372.0
38	3088707.0
39	643230.0
40	3113.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.172079443278214	28.074276986833507	28.981701923709092	21.771941646179187
2	21.821861932585985	28.74113135866522	28.990903876136098	20.4461028326127
3	19.822178685052	27.62518993538592	30.710935756685227	21.841695622876863
4	20.763686285033714	26.736401090742984	30.764033344062835	21.735879280160468
5	21.623647233560707	27.67590454703542	28.879722782934376	21.820725436469502
6	21.694806554918546	27.804340828586323	28.6493195809971	21.85153303549803
7	21.85214405491549	28.091776582949674	28.12331740527916	21.932761956855675
8	21.80850504812022	28.439532174205056	28.03921669265943	21.712746085015286
9	21.961015494719206	28.29659029168356	28.045326886834072	21.697067326763165
10-14	21.922682580545192	28.300776996732026	27.949018006253418	21.82752241646937
15-19	21.915616752001636	28.236676171684728	28.07403991129953	21.773667165014103
20-24	21.798337685013625	28.163414943530807	28.20568037867562	21.83256699277995
25-29	21.859209883459044	28.16454410741428	28.028394316737316	21.94785169238936
30-34	21.870056700157864	28.182366321782865	28.065326774406497	21.882250203652774
35-39	21.817399046760826	28.211797905083262	28.119355555376323	21.85144749277958
40-44	21.862084118798794	28.19582830158843	28.06392876197934	21.878158817633434
45-49	21.97560908248591	28.090549655959407	28.022653178290824	21.91118808326386
50-54	21.91772599103072	28.192704770326355	28.03241971265956	21.857149525983356
55-59	21.910872797244448	28.147171603336947	28.006021229747457	21.93593436967115
60-64	21.89005658773029	28.168530398093814	28.04231089498947	21.899102119186427
65-69	21.85576128986688	28.166887977899673	28.066485267222006	21.91086546501144
70-74	21.841744504430256	28.203351172656244	28.007839623533826	21.947064699379666
75-79	21.836758585983752	28.141000307220565	28.049054105280604	21.973187001515086
80-84	21.781603085208122	28.227945926247997	28.053661191688278	21.936789796855596
85-89	21.722011583461708	28.242681270519558	28.002599521009657	22.032707625009074
90-94	21.052362690052504	27.849125952202797	29.49102021091156	21.607491146833134
95-99	21.692986396177535	28.231862195892806	28.209833308719528	21.865318099210132
100-104	21.809887294451972	28.321532041727888	28.043179386181283	21.82540127763886
105-109	21.686586126881036	28.3252025965096	28.14842944324448	21.83978183336488
110-114	21.703829711945456	28.126921144754885	28.225709329557947	21.943539813741715
115-119	21.70765680860548	28.240291106197617	28.099991400842324	21.952060684354574
120-124	21.691312763942392	28.255252798587883	28.088113420879456	21.965321016590266
125-129	21.654114975506193	28.217473290780525	28.165473646930867	21.96293808678241
130-134	21.766433276414563	28.252486366050643	28.060893528475635	21.92018682905916
135-139	21.86280146666789	28.1920492638062	27.994572328434263	21.950576941091647
140-144	21.841850857898375	28.219641023407977	28.064808323823502	21.873699794870145
145-149	21.79367480107893	28.276184195665476	28.065503125651208	21.864637877604384
150-154	21.740486941336986	28.336053089098932	28.08841802040245	21.835041949161635
155-159	21.735368941818376	28.29050485443481	28.145149840591078	21.828976363155736
160-164	21.654106804011928	28.4521372800839	28.142811222849794	21.75094469305438
165-169	21.588062130209213	28.440099287363086	28.164438802637747	21.80739977978996
170-174	21.549063329167303	28.506671831223173	28.243043792102295	21.70122104750723
175-178	21.487317257056485	28.62057777406187	28.162880110477566	21.729224858404077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.5
13	10.5
14	23.0
15	41.5
16	98.5
17	201.0
18	408.5
19	815.0
20	1627.5
21	2878.5
22	4928.0
23	8454.5
24	13793.5
25	21257.5
26	31141.5
27	43983.5
28	59868.0
29	78681.5
30	100437.0
31	124615.0
32	151341.0
33	179693.5
34	208643.5
35	238146.0
36	267540.0
37	296602.0
38	323351.0
39	347577.5
40	369176.5
41	385537.0
42	395843.0
43	399804.0
44	397748.0
45	389211.0
46	373907.0
47	351847.0
48	324584.5
49	295295.5
50	265180.0
51	234996.0
52	206146.5
53	179304.0
54	154688.0
55	133743.0
56	116289.0
57	100920.5
58	88598.0
59	78320.0
60	69127.0
61	61222.5
62	54043.0
63	47398.0
64	41038.0
65	35103.0
66	29289.0
67	24024.0
68	19466.0
69	15246.0
70	11696.0
71	8874.0
72	6429.0
73	4553.0
74	3192.5
75	2128.5
76	1396.5
77	866.0
78	522.0
79	325.0
80	185.5
81	108.0
82	57.0
83	38.5
84	22.0
85	6.0
86	2.5
87	1.5
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1009.0
92-93	1876.0
94-95	2719.0
96-97	3444.0
98-99	4325.0
100-101	5177.0
102-103	6227.0
104-105	8149.0
106-107	8229.0
108-109	9958.0
110-111	11812.0
112-113	10052.0
114-115	10441.0
116-117	11361.0
118-119	12403.0
120-121	13396.0
122-123	15496.0
124-125	13992.0
126-127	14089.0
128-129	15375.0
130-131	16370.0
132-133	17558.0
134-135	106462.0
136-137	72642.0
138-139	70529.0
140-141	71191.0
142-143	80118.0
144-145	85064.0
146-147	131527.0
148-149	79138.0
150-151	76825.0
152-153	74982.0
154-155	126556.0
156-157	78069.0
158-159	82382.0
160-161	79880.0
162-163	87123.0
164-165	85206.0
166-167	98375.0
168-169	82028.0
170-171	86060.0
172-173	169895.0
174-175	94931.0
176-177	245204.0
178-179	5805401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.35333930382549
#Duplication Level	Percentage of total
1	86.09354194559586
2	10.721843301911552
3	1.9907291568383987
4	0.5543944782468254
5	0.2231152873186227
6	0.12047686152374909
7	0.07769955029082745
8	0.07608763223123252
9	0.033555959796032625
>10	0.1002169033800341
>50	0.0044173227159719185
>100	0.003921600150880173
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2220388349277274E-5	1.2220388349277274E-5	0.0	2.444077669855455E-5	0.0	0.0
2	2.3218737863626822E-4	2.444077669855455E-5	0.0	2.444077669855455E-5	0.0	0.0
3	2.3218737863626822E-4	2.444077669855455E-5	1.2220388349277274E-5	6.110194174638637E-5	0.0	0.0
4	2.444077669855455E-4	2.444077669855455E-5	1.2220388349277274E-5	7.332233009566364E-5	0.0	0.0
5	2.444077669855455E-4	2.444077669855455E-5	1.2220388349277274E-5	7.332233009566364E-5	0.0	0.0
6	2.444077669855455E-4	3.666116504783182E-5	2.444077669855455E-5	7.332233009566364E-5	0.0	0.0
7	2.444077669855455E-4	3.666116504783182E-5	3.666116504783182E-5	8.554271844494092E-5	1.2220388349277274E-5	0.0
8	2.5662815533482276E-4	3.666116504783182E-5	3.666116504783182E-5	8.554271844494092E-5	1.2220388349277274E-5	0.0
9	2.5662815533482276E-4	3.666116504783182E-5	3.666116504783182E-5	9.77631067942182E-5	1.2220388349277274E-5	0.0
10-14	2.5662815533482276E-4	4.88815533971091E-5	6.843417475595273E-5	1.0998349514349548E-4	1.2220388349277274E-5	0.0
15-19	2.615163106745337E-4	8.309864077508547E-5	1.0998349514349548E-4	1.5886504854060456E-4	1.2220388349277274E-5	0.0
20-24	2.7862485436352187E-4	1.0509533980378457E-4	1.0998349514349548E-4	2.321873786362682E-4	1.710854368898818E-5	0.0
25-29	2.884011650429437E-4	1.148716504832064E-4	1.1242757281335094E-4	2.590722330046782E-4	3.177300970812092E-5	0.0
30-34	3.348386407701973E-4	1.246479611626282E-4	1.4420058252147185E-4	3.1039786407164276E-4	5.865786407653091E-5	0.0
35-39	4.057168931960055E-4	1.3442427184205002E-4	1.4664466019132728E-4	3.2750640776063096E-4	6.599009708609728E-5	9.77631067942182E-6
40-44	4.594866019328256E-4	1.6864135922002636E-4	1.6130912621046002E-4	3.6416757280846276E-4	9.043087378465182E-5	3.421708737797636E-5
45-49	5.670260194064656E-4	2.004143689281473E-4	2.0285844659800276E-4	4.08160970865861E-4	1.1242757281335094E-4	1.246479611626282E-4
50-54	6.599009708609727E-4	2.1019067960756913E-4	2.590722330046782E-4	4.5704252426297006E-4	1.417565048516164E-4	1.8574990290901456E-4
55-59	7.258910679470701E-4	2.3218737863626824E-4	2.8106893203337733E-4	4.88815533971091E-4	1.955262135884364E-4	2.2485514562670187E-4
60-64	7.967693203728783E-4	2.4685184465540093E-4	2.8106893203337733E-4	5.425852427079109E-4	2.1996699028699096E-4	2.444077669855455E-4
65-69	8.847561164876746E-4	2.5662815533482276E-4	2.8106893203337733E-4	5.890227184351646E-4	2.3951961164583456E-4	2.9084524271279916E-4
70-74	0.0014664466019132729	2.5662815533482276E-4	3.055097087319319E-4	6.550128155212619E-4	2.5173999999511185E-4	3.128419417414982E-4
75-79	0.011316079611430755	2.712926213539555E-4	3.299504854304864E-4	7.185588349375037E-4	3.128419417414982E-4	3.1773009708120913E-4
80-84	0.015612768155036644	3.0306563106207644E-4	3.617234951386073E-4	7.698844660044682E-4	4.5704252426297006E-4	3.2750640776063096E-4
85-89	0.015644541164744764	3.6172349513860735E-4	3.714998058180292E-4	7.943252427030228E-4	7.014502912485156E-4	3.3972679610990824E-4
90-94	0.01569342271814187	4.1060504853571644E-4	4.057168931960055E-4	8.11433786392011E-4	7.087825242580819E-4	3.421708737797637E-4
95-99	0.015715419417170574	4.4726621358354824E-4	4.1549320387542735E-4	8.823120388178193E-4	7.087825242580819E-4	3.421708737797637E-4
100-104	0.01572763980551985	4.741510679519582E-4	4.2038135921513827E-4	9.311935922149282E-4	7.185588349375037E-4	3.421708737797637E-4
105-109	0.0157471924268787	4.814833009615245E-4	4.374899029041264E-4	9.702988349326155E-4	7.210029126073591E-4	3.421708737797637E-4
110-114	0.015822958834644216	5.034799999902237E-4	4.4482213591369283E-4	9.77631067942182E-4	7.210029126073591E-4	3.421708737797637E-4
115-119	0.015871840388041326	5.328089320284892E-4	4.912596116409464E-4	0.0010094040776503028	7.210029126073591E-4	3.421708737797637E-4
120-124	0.01592561009677814	5.572497087270437E-4	5.279207766887783E-4	0.00104850932036799	7.307792232867809E-4	3.446149514496191E-4
125-129	0.016069810679299613	5.621378640667547E-4	5.474733980476218E-4	0.0010656178640569784	7.381114562963473E-4	3.7394388348788453E-4
130-134	0.016243340193859358	5.743582524160318E-4	5.499174757174774E-4	0.0011022790291048102	7.625522329949019E-4	3.910524271768728E-4
135-139	0.01636798815502198	5.816904854255982E-4	5.499174757174774E-4	0.0011291638834732202	8.016574757125893E-4	4.0327281552615E-4
140-144	0.016492636116184605	5.939108737748755E-4	5.572497087270437E-4	0.0011658250485210519	8.212100970714329E-4	4.2282543688499367E-4
145-149	0.016639280776375935	5.987990291145864E-4	5.670260194064655E-4	0.0011731572815306182	8.358745630905655E-4	4.277135922247046E-4
150-154	0.016756596504528994	5.987990291145864E-4	5.816904854255982E-4	0.001187821747549751	8.432067961001318E-4	4.277135922247046E-4
155-159	0.016925237863749026	6.085753397940082E-4	5.963549514447308E-4	0.00120981844657845	8.432067961001318E-4	4.277135922247046E-4
160-164	0.01728696135888763	6.23239805813141E-4	6.110194174638636E-4	0.0012244829125975828	8.480949514398428E-4	4.277135922247046E-4
165-167	0.017450714562767946	6.313867313793259E-4	6.599009708609728E-4	0.0012424061488431894	8.676475727986864E-4	4.3993398057398187E-4
>>END_MODULE
