##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293869
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	565460
Total Bases	97 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.40567325717115	39.0	39.0	39.0	37.0	39.0
2	40.163137976161	41.0	40.0	41.0	38.0	41.0
3	40.20366073639161	41.0	40.0	41.0	38.0	41.0
4	40.22456053478584	41.0	40.0	41.0	38.0	41.0
5	40.22239769391292	41.0	40.0	41.0	38.0	41.0
6	40.18731475259081	41.0	40.0	41.0	38.0	41.0
7	40.2050525236091	41.0	40.0	41.0	38.0	41.0
8	40.18224454426485	41.0	40.0	41.0	38.0	41.0
9	40.16550772822127	41.0	40.0	41.0	38.0	41.0
10-14	40.083610158101365	41.0	40.0	41.0	38.0	41.0
15-19	39.934468574258126	41.0	40.0	41.0	38.0	41.0
20-24	39.76493934142114	40.6	39.8	41.0	37.8	41.0
25-29	39.617382308209244	40.0	39.0	41.0	37.0	41.0
30-34	39.432499911576414	40.0	39.0	41.0	36.4	41.0
35-39	39.37573373890284	40.0	38.8	41.0	35.8	41.0
40-44	39.0617033919287	40.0	38.0	41.0	35.0	41.0
45-49	38.430506136596755	39.4	36.4	41.0	35.0	41.0
50-54	37.703174052983414	38.2	35.2	40.4	34.6	41.0
55-59	36.91379549393414	36.6	35.0	39.2	34.0	41.0
60-64	35.91593145403742	35.4	34.6	37.8	33.4	40.0
65-69	35.493943691861496	35.0	34.8	36.6	33.6	39.0
70-74	35.280907225975305	35.0	35.0	36.0	34.0	37.2
75-79	35.06853641283203	35.0	35.0	35.2	33.0	36.8
80-84	34.97719095957274	35.0	35.0	35.0	33.0	36.0
85-89	34.78164892300074	35.0	34.6	35.0	32.4	36.2
90-94	39.613900152578154	40.6	39.8	40.6	37.8	40.6
95-99	39.86843999621774	41.0	40.0	41.0	38.0	41.0
100-104	39.75011043071634	41.0	40.0	41.0	37.0	41.0
105-109	39.57769398774693	40.4	39.2	41.0	36.6	41.0
110-114	39.36772518656003	40.0	38.8	41.0	36.0	41.0
115-119	39.09152954100357	40.0	38.0	41.0	35.0	41.0
120-124	38.730452574751936	40.0	38.0	41.0	35.0	41.0
125-129	38.11753850040766	39.4	36.8	40.4	34.4	40.6
130-134	38.14094185850687	39.0	36.0	40.6	34.2	41.0
135-139	38.11720240859937	39.0	35.6	41.0	34.6	41.0
140-144	37.44135384726226	37.6	35.0	40.2	34.0	41.0
145-149	36.65068726658369	36.2	35.0	39.0	34.0	41.0
150-154	35.89647623381806	35.2	35.0	37.2	33.8	39.4
155-159	35.2730188176906	35.0	35.0	36.2	33.0	37.8
160-164	34.84058829768062	35.0	35.0	35.4	33.0	36.6
165-169	34.585974596434696	35.0	34.4	35.0	33.0	36.0
170-174	34.454153737620075	35.0	34.0	35.0	33.0	35.8
175-178	34.14440217767249	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	19.0
31	151.0
32	848.0
33	3628.0
34	10493.0
35	28071.0
36	76266.0
37	185966.0
38	219015.0
39	40898.0
40	103.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.211933646942313	28.017543239132742	29.069076504085167	21.701446609839774
2	21.84734552399816	28.783998868178116	28.94351501432462	20.425140593499098
3	19.872316344215328	27.498143104728896	30.758143812117567	21.87139673893821
4	20.740459095249886	26.783857390443178	30.68227637675521	21.79340713755173
5	21.684292434478124	27.621051886959293	28.744208255225836	21.95044742333675
6	21.87316521062498	27.679941994128676	28.56806847522371	21.878824320022634
7	21.855480493757295	28.103137268772326	28.175998302267182	21.865383935203198
8	21.919676015986983	28.38167155943833	28.007816644855517	21.690835779719166
9	21.990945424963744	28.23471156226789	28.00534078449404	21.769002228274324
10-14	21.9925016800481	28.277473207653948	27.876313090227427	21.853712022070525
15-19	21.919074735613485	28.212004385809784	28.026279489265377	21.842641389311357
20-24	21.81399214798571	28.12220139355569	28.15778304389347	21.90602341456513
25-29	21.8520850281187	28.141654582110142	28.001874579987973	22.004385809783187
30-34	21.90032893573374	28.13967389382096	28.02189367948219	21.93810349096311
35-39	21.828069182612385	28.176175149435856	28.106072931772363	21.889682736179395
40-44	21.90641247833622	28.169702543062286	27.99731192303611	21.926573055565378
45-49	22.046298588759594	28.06706044636225	27.914264492625474	21.97237647225268
50-54	21.978919817493722	28.145226894917414	27.9581933293248	21.917659958264068
55-59	21.949952251264456	28.069571676157462	27.984720404626323	21.995755667951755
60-64	21.95221589502352	28.129805821808795	27.93325787854136	21.98472040462632
65-69	21.942560039613763	28.117532628302623	27.982562869168465	21.95734446291515
70-74	21.876560676263573	28.193612280267395	27.914016906589325	22.01581013687971
75-79	21.858840589962156	28.11654228415803	27.967672337565872	22.05694478831394
80-84	21.852261875287375	28.166519294026106	27.935380044565488	22.045838786121035
85-89	21.78003749159976	28.1960174017614	27.90085947723977	22.12308562939907
90-94	21.03783690467262	27.83143552132403	29.507840060224343	21.622887513779006
95-99	21.727531281927106	28.23926275685944	28.17596610584701	21.857239855366444
100-104	21.821878593415693	28.311153111587835	28.034703799751306	21.83226449524517
105-109	21.661432455235776	28.400461917383264	28.096316592379218	21.841789035001746
110-114	21.703779373972225	28.15056986040022	28.167049895709496	21.978600869918065
115-119	21.69801991685543	28.25640943257132	28.080338575832382	21.965232074740864
120-124	21.634372886407665	28.27083787098919	28.072948310845664	22.021840931757477
125-129	21.67040770415149	28.22563329392686	28.11590178904433	21.988057212877322
130-134	21.762573423096963	28.287212295988745	27.97979089997984	21.970423380934452
135-139	21.89732371551822	28.15851254121619	27.966711969743763	21.977451773521828
140-144	21.88471362600061	28.206349415185443	27.99317356108178	21.915763397732164
145-149	21.79875277560884	28.25692343854755	28.038773031329594	21.90555075451402
150-154	21.69351687727524	28.38494514011982	28.087535974993806	21.834002007611133
155-159	21.694938652248656	28.30822665565003	28.122841719493273	21.87399297260804
160-164	21.593234159232537	28.47671391720588	28.175421781641667	21.754630141919915
165-169	21.54037675719729	28.4814882662059	28.139262576522146	21.838872400074667
170-174	21.500470566750412	28.576691531516218	28.176732395333932	21.746105506399438
175-178	21.472201729948846	28.666261619710603	28.177543084552862	21.68399356578769
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	2.0
16	6.0
17	16.5
18	28.0
19	61.5
20	114.5
21	186.5
22	325.5
23	539.5
24	881.5
25	1386.0
26	2063.5
27	2917.0
28	3999.0
29	5361.0
30	6815.5
31	8389.0
32	10175.5
33	12167.0
34	14212.0
35	16144.5
36	18156.5
37	20224.0
38	22165.5
39	23965.0
40	25551.0
41	26572.5
42	27233.0
43	27801.0
44	27768.5
45	26901.0
46	25757.5
47	24381.0
48	22577.0
49	20665.5
50	18634.0
51	16634.0
52	14536.0
53	12543.0
54	10965.5
55	9564.0
56	8227.5
57	7074.0
58	6235.0
59	5454.5
60	4811.0
61	4287.5
62	3749.5
63	3270.0
64	2891.5
65	2488.5
66	2025.0
67	1621.5
68	1308.5
69	1058.5
70	772.5
71	579.5
72	432.5
73	291.5
74	203.0
75	129.0
76	88.5
77	53.5
78	30.0
79	15.5
80	8.5
81	5.5
82	2.5
83	1.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	109.0
92-93	90.0
94-95	189.0
96-97	224.0
98-99	292.0
100-101	326.0
102-103	344.0
104-105	437.0
106-107	449.0
108-109	507.0
110-111	576.0
112-113	669.0
114-115	661.0
116-117	703.0
118-119	790.0
120-121	796.0
122-123	839.0
124-125	886.0
126-127	959.0
128-129	979.0
130-131	1046.0
132-133	1138.0
134-135	5316.0
136-137	5048.0
138-139	5020.0
140-141	4965.0
142-143	5259.0
144-145	5792.0
146-147	5728.0
148-149	5969.0
150-151	5673.0
152-153	5627.0
154-155	5622.0
156-157	5491.0
158-159	5677.0
160-161	5871.0
162-163	6031.0
164-165	6479.0
166-167	6516.0
168-169	6800.0
170-171	7109.0
172-173	7212.0
174-175	7837.0
176-177	19120.0
178-179	408289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.27235620150446
#Duplication Level	Percentage of total
1	98.57372424809952
2	1.353206505127913
3	0.061120922892422144
4	0.0026134815720784206
5	0.0028408865618517096
6	0.0015393777421039388
7	0.0
8	0.0
9	0.0
>10	0.004954578004109441
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
3	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
4	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
5	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
6	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
7	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
8	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
9	3.5369433735365894E-4	0.0	0.0	0.0	0.0	0.0
10-14	3.5369433735365894E-4	0.0	1.0610830120609768E-4	0.0	0.0	0.0
15-19	3.5369433735365894E-4	0.0	1.7684716867682947E-4	0.0	0.0	0.0
20-24	3.5369433735365894E-4	0.0	1.7684716867682947E-4	0.0	0.0	0.0
25-29	3.5369433735365894E-4	0.0	1.7684716867682947E-4	2.1221660241219537E-4	1.4147773494146358E-4	0.0
30-34	3.5369433735365894E-4	0.0	1.7684716867682947E-4	3.5369433735365894E-4	1.7684716867682947E-4	0.0
35-39	3.5369433735365894E-4	0.0	1.7684716867682947E-4	3.5369433735365894E-4	2.475860361475613E-4	0.0
40-44	3.5369433735365894E-4	0.0	1.7684716867682947E-4	5.305415060304885E-4	3.5369433735365894E-4	0.0
45-49	9.549747108548792E-4	0.0	3.183249036182931E-4	7.781275421780496E-4	4.951720722951227E-4	0.0
50-54	0.0012379301807378064	0.0	3.890637710890248E-4	8.842358433841475E-4	5.305415060304885E-4	0.0
55-59	0.0012379301807378064	1.4147773494146358E-4	5.305415060304885E-4	9.196052771195133E-4	5.305415060304885E-4	0.0
60-64	0.0012379301807378064	1.7684716867682947E-4	5.305415060304885E-4	0.001061083012060977	5.659109397658544E-4	0.0
65-69	0.0012379301807378064	1.7684716867682947E-4	7.073886747073179E-4	0.001061083012060977	7.073886747073179E-4	0.0
70-74	0.0015916245180914654	1.7684716867682947E-4	7.073886747073179E-4	0.001061083012060977	7.073886747073179E-4	1.7684716867682947E-4
75-79	0.012060976903759771	1.7684716867682947E-4	7.073886747073179E-4	0.0012379301807378064	8.488664096487815E-4	1.7684716867682947E-4
80-84	0.01644678668694514	1.7684716867682947E-4	7.073886747073179E-4	0.0012379301807378064	9.549747108548792E-4	1.7684716867682947E-4
85-89	0.01644678668694514	1.7684716867682947E-4	7.073886747073179E-4	0.0014147773494146358	0.0012379301807378064	1.7684716867682947E-4
90-94	0.01644678668694514	2.8295546988292715E-4	7.073886747073179E-4	0.001697732819297563	0.0012379301807378064	1.7684716867682947E-4
95-99	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.001768471686768295	0.0012379301807378064	1.7684716867682947E-4
100-104	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.0018038411205036607	0.0012379301807378064	1.7684716867682947E-4
105-109	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.0019453188554451246	0.0012379301807378064	1.7684716867682947E-4
110-114	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.0019453188554451246	0.0012379301807378064	1.7684716867682947E-4
115-119	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.0019806882891804903	0.0012379301807378064	1.7684716867682947E-4
120-124	0.01644678668694514	3.5369433735365894E-4	7.073886747073179E-4	0.0021575354578573197	0.0012379301807378064	1.7684716867682947E-4
125-129	0.01648215612068051	3.5369433735365894E-4	7.073886747073179E-4	0.0022990131927987834	0.0012379301807378064	1.7684716867682947E-4
130-134	0.01662363385562197	3.5369433735365894E-4	7.073886747073179E-4	0.0023343826265341495	0.0012379301807378064	1.7684716867682947E-4
135-139	0.01665900328935734	3.5369433735365894E-4	7.073886747073179E-4	0.0024758603614756128	0.0012379301807378064	1.7684716867682947E-4
140-144	0.0169065893255049	3.5369433735365894E-4	7.073886747073179E-4	0.0024758603614756128	0.0012732996144731723	1.7684716867682947E-4
145-149	0.01715417536165246	3.5369433735365894E-4	7.427581084426838E-4	0.0024758603614756128	0.0014147773494146358	1.7684716867682947E-4
150-154	0.017366391964064653	3.5369433735365894E-4	8.842358433841475E-4	0.0025819686626817103	0.0014147773494146358	1.7684716867682947E-4
155-159	0.01750786969900612	3.5369433735365894E-4	8.842358433841475E-4	0.002652707530152442	0.0014147773494146358	1.7684716867682947E-4
160-164	0.01750786969900612	3.5369433735365894E-4	8.842358433841475E-4	0.002652707530152442	0.0014147773494146358	1.7684716867682947E-4
165-167	0.017625767811457336	3.5369433735365894E-4	8.842358433841474E-4	0.002652707530152442	0.0014147773494146358	1.7684716867682947E-4
>>END_MODULE
