##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293876
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7939233
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.985823693548234	41.0	40.0	41.0	38.0	41.0
2	40.1313314522952	41.0	40.0	41.0	38.0	41.0
3	40.164531132919265	41.0	40.0	41.0	38.0	41.0
4	40.173895261670744	41.0	40.0	41.0	38.0	41.0
5	40.15278604368961	41.0	40.0	41.0	38.0	41.0
6	40.13022882689046	41.0	40.0	41.0	38.0	41.0
7	40.140631343103294	41.0	40.0	41.0	38.0	41.0
8	40.1159846297495	41.0	40.0	41.0	38.0	41.0
9	40.0934092751781	41.0	40.0	41.0	38.0	41.0
10-14	40.01948747441976	41.0	40.0	41.0	38.0	41.0
15-19	39.85911875870125	41.0	40.0	41.0	38.0	41.0
20-24	39.628152442433674	40.6	39.2	41.0	37.0	41.0
25-29	39.45524722602296	40.2	39.0	41.0	36.0	41.0
30-34	39.363594720044105	40.6	38.8	41.0	35.0	41.0
35-39	38.94315130945269	40.0	37.4	41.0	35.0	41.0
40-44	38.296732089863085	39.2	36.0	41.0	35.0	41.0
45-49	37.53791460711633	37.6	35.0	40.4	34.6	41.0
50-54	36.78677227384559	36.4	35.0	39.2	34.0	41.0
55-59	35.97559867558995	35.2	34.8	37.6	33.6	40.0
60-64	35.56147459080745	35.0	35.0	36.4	33.8	39.0
65-69	35.30582082677256	35.0	35.0	36.0	34.0	37.4
70-74	35.10342356749071	35.0	35.0	35.0	34.0	37.0
75-79	35.023636817309686	35.0	35.0	35.0	33.0	36.0
80-84	34.84322135400233	35.0	34.6	35.0	32.6	36.2
85-89	39.95846072284313	41.0	40.0	41.0	38.0	41.0
90-94	39.89292201975525	41.0	40.0	41.0	38.0	41.0
95-99	39.75021930571008	41.0	40.0	41.0	37.0	41.0
100-104	39.57241360348722	41.0	39.2	41.0	36.4	41.0
105-109	39.313507434902746	40.0	38.6	41.0	35.4	41.0
110-114	39.07290723650742	40.0	38.0	41.0	35.0	41.0
115-119	38.58414464772511	39.6	37.2	40.6	34.6	41.0
120-124	38.34272051251946	39.6	36.2	41.0	34.8	41.0
125-129	37.95716839041612	39.0	35.0	41.0	34.8	41.0
130-134	37.33566779259157	37.2	35.0	40.4	34.0	41.0
135-139	36.61703980460379	36.0	35.0	39.0	34.0	41.0
140-144	35.93329771277912	35.0	35.0	37.0	34.0	39.4
145-149	35.37532645487772	35.0	35.0	36.0	34.0	38.0
150-154	34.989377086489476	35.0	35.0	35.4	33.6	36.6
155-159	34.747461231132846	35.0	35.0	35.0	33.0	36.0
160-164	34.62114230174048	35.0	35.0	35.0	33.0	36.0
165-168	34.314308885074084	35.0	34.5	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	35.0
30	465.0
31	3466.0
32	17597.0
33	65232.0
34	184521.0
35	469728.0
36	1223028.0
37	2509509.0
38	2755809.0
39	703219.0
40	6623.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.83881830902305	26.981359534353004	27.518073849199286	22.66174830742466
2	22.544570741279415	27.260643439989735	27.45979366016843	22.73499215856242
3	22.53819732964129	27.416855003499705	27.404889112084252	22.64005855477475
4	22.920085605246754	27.31441437730824	27.110628948665443	22.654871068779567
5	22.636871848955685	27.576215485803225	27.148743461742463	22.638169203498627
6	22.594210297140794	27.541287678545267	27.330687989633258	22.53381403468068
7	22.999828824774383	27.31069865313186	27.05489308601977	22.634579436073988
8	22.727825219388322	27.397759959935676	27.02863110328164	22.84578371739436
9	22.6080025614565	27.41283698311915	27.236333786903593	22.74282666852075
10-14	22.705480491629356	27.402052566035028	27.24822158513297	22.644245357202642
15-19	22.596825159306952	27.265273610183755	27.408058183958072	22.729843046551224
20-24	22.606685053833285	27.315054237607082	27.25052911282488	22.82773159573475
25-29	22.675643856276796	27.34113736175774	27.23078413242186	22.752434649543602
30-34	22.636060687474473	27.32410800892227	27.28042368828324	22.75940761532002
35-39	22.59911253391858	27.364562798446652	27.289217988689842	22.74710667894493
40-44	22.73094138942641	27.278118679726365	27.178653152011034	22.812286778836192
45-49	22.77805929111792	27.310529871084526	27.16927441227635	22.742136425521206
50-54	22.748341055111997	27.28071590794728	27.1768645661363	22.794078470804422
55-59	22.76883169948533	27.302617267940114	27.155325961588485	22.773225070986076
60-64	22.72511966836091	27.269236209593544	27.211973751116762	22.79367037092878
65-69	22.665622737108233	27.335819467699206	27.205937903573307	22.792619891619253
70-74	22.701394454602855	27.274128369831192	27.194271789226992	22.83020538633896
75-79	22.673127240376996	27.300992425842647	27.20131277164935	22.824567562131
80-84	22.65368455617816	27.286383961775652	27.175657900454613	22.88427358159157
85-89	22.630390114511062	27.359806671500884	27.36069340703315	22.649109806954904
90-94	22.680577774300883	27.429560436058072	27.219508102488287	22.67035368715276
95-99	22.529271936603728	27.45917401516238	27.385204907590815	22.626349140643075
100-104	22.59977124248711	27.255373583892283	27.394853834941586	22.750001338679024
105-109	22.616089345162532	27.32729455252717	27.25708278632458	22.799533315985716
110-114	22.5415032223868	27.3858936338544	27.29721741796764	22.775385725791153
115-119	22.565833974506994	27.334299967099646	27.323944997624373	22.775921060768987
120-124	22.562553426644236	27.364576175969813	27.296377450176557	22.776492947209395
125-129	22.65870243496932	27.34013513874252	27.214999434474468	22.78616299181369
130-134	22.755513194142097	27.335516776557927	27.196451054916864	22.712518974383112
135-139	22.701846100333125	27.354854172459437	27.215170100818376	22.728129626389055
140-144	22.61867680280454	27.457740898230586	27.25232643254157	22.671255866423305
145-149	22.630867232052015	27.426141770646833	27.293178415070905	22.649812582230247
150-154	22.556558297539304	27.54016632869454	27.33122352906333	22.572051844702827
155-159	22.486321504892807	27.55555912237971	27.371090190885912	22.587029181841576
160-164	22.489660331962995	27.61759197582108	27.395124448841013	22.497623243374907
165-168	22.402064236983442	27.702507003941427	27.387683037084216	22.50774572199092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	3.5
13	5.0
14	11.0
15	33.0
16	75.0
17	176.0
18	372.5
19	800.5
20	1649.0
21	3143.0
22	5520.0
23	8962.5
24	13927.0
25	20605.0
26	28930.0
27	39210.0
28	51534.5
29	65639.5
30	81639.5
31	99578.0
32	119266.0
33	140166.5
34	162487.5
35	186348.5
36	210958.0
37	235850.5
38	259422.5
39	282081.0
40	303345.5
41	321456.0
42	335617.0
43	344635.0
44	349634.0
45	349375.5
46	343785.0
47	332660.5
48	318371.5
49	301783.5
50	281123.0
51	259817.0
52	238074.5
53	216344.5
54	195690.5
55	176468.5
56	158930.0
57	143314.0
58	129594.0
59	117038.0
60	105796.5
61	95354.0
62	85412.5
63	76280.5
64	66961.5
65	57560.5
66	48809.0
67	40699.0
68	33112.5
69	26082.5
70	20070.0
71	15152.5
72	11049.0
73	7727.5
74	5304.5
75	3504.5
76	2273.5
77	1409.5
78	826.5
79	476.0
80	270.0
81	155.5
82	68.0
83	31.5
84	17.5
85	8.0
86	3.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	856.0
92-93	1881.0
94-95	2995.0
96-97	3888.0
98-99	5061.0
100-101	6072.0
102-103	6994.0
104-105	9154.0
106-107	8749.0
108-109	9322.0
110-111	10068.0
112-113	10608.0
114-115	11505.0
116-117	13027.0
118-119	12502.0
120-121	13387.0
122-123	13884.0
124-125	14647.0
126-127	15093.0
128-129	46378.0
130-131	74203.0
132-133	76671.0
134-135	80986.0
136-137	79410.0
138-139	78180.0
140-141	84047.0
142-143	80177.0
144-145	76865.0
146-147	76273.0
148-149	77995.0
150-151	76212.0
152-153	80242.0
154-155	81216.0
156-157	92501.0
158-159	83077.0
160-161	85083.0
162-163	93253.0
164-165	96354.0
166-167	265773.0
168-169	5984644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.18117775994871
#Duplication Level	Percentage of total
1	92.72733315576009
2	6.33551316827881
3	0.7166135956911391
4	0.1303759607025074
5	0.03792444315876207
6	0.01959725290641187
7	0.011417014708504251
8	0.005222065691563793
9	0.00525471243931889
>10	0.010748630662895282
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.5191350348327098E-5	1.2595675174163549E-5	0.0	0.0
2	0.0	0.0	3.778702552249065E-5	1.2595675174163549E-5	0.0	0.0
3	1.2595675174163549E-5	2.5191350348327098E-5	3.778702552249065E-5	5.0382700696654195E-5	0.0	0.0
4	1.2595675174163549E-5	5.0382700696654195E-5	3.778702552249065E-5	6.297837587081775E-5	0.0	0.0
5	1.2595675174163549E-5	5.0382700696654195E-5	3.778702552249065E-5	6.297837587081775E-5	0.0	0.0
6	2.5191350348327098E-5	5.0382700696654195E-5	6.297837587081775E-5	6.297837587081775E-5	0.0	0.0
7	3.778702552249065E-5	5.0382700696654195E-5	6.297837587081775E-5	6.297837587081775E-5	0.0	0.0
8	3.778702552249065E-5	6.297837587081775E-5	7.55740510449813E-5	6.297837587081775E-5	0.0	0.0
9	3.778702552249065E-5	6.297837587081775E-5	7.55740510449813E-5	7.55740510449813E-5	1.2595675174163549E-5	0.0
10-14	3.778702552249065E-5	8.313145614947944E-5	9.068886125397757E-5	1.3099502181130094E-4	1.2595675174163547E-5	0.0
15-19	4.786356566182149E-5	1.1336107656747195E-4	1.1839934663713737E-4	1.8137772250795514E-4	1.2595675174163547E-5	0.0
20-24	6.297837587081775E-5	1.2847588677646822E-4	1.259567517416355E-4	2.5947090858776913E-4	1.2595675174163547E-5	0.0
25-29	7.053578097531589E-5	1.763394524382897E-4	1.3855242691579906E-4	3.224492844585869E-4	1.2595675174163547E-5	0.0
30-34	1.3855242691579906E-4	1.9649253271695135E-4	1.3855242691579906E-4	3.7535112019007383E-4	3.778702552249065E-5	0.0
35-39	2.51913503483271E-4	2.2672215313494386E-4	1.435906969854645E-4	4.2069555081706254E-4	7.053578097531589E-5	1.2595675174163547E-5
40-44	3.551980399114121E-4	2.6450917865743456E-4	1.5366723712479532E-4	4.509251712350551E-4	1.259567517416355E-4	1.2595675174163547E-5
45-49	4.635208464092187E-4	2.796239888664308E-4	1.7130118236862428E-4	4.8367392668788035E-4	2.267221531349439E-4	1.2595675174163547E-5
50-54	5.919967331856869E-4	3.249684194934196E-4	1.7633945243828968E-4	5.365757624193673E-4	3.123727443192561E-4	3.0229620417992515E-5
55-59	6.549751090565046E-4	3.5267890487657936E-4	1.9145426264728595E-4	5.668053828373598E-4	3.9046593039907E-4	5.290183573148691E-5
60-64	7.683361856239765E-4	3.577171749462448E-4	2.0153080278661675E-4	6.045924083598504E-4	4.559634413047205E-4	8.313145614947944E-5
65-69	0.0015669019916659456	3.7283198515524103E-4	2.2420301810011117E-4	6.297837587081775E-4	5.239800872452037E-4	1.259567517416355E-4
70-74	0.015336494092061536	3.7787025522490646E-4	2.393178283091074E-4	6.524559740216718E-4	5.945158682205196E-4	1.435906969854645E-4
75-79	0.02108516024154978	4.156572807473971E-4	2.6450917865743456E-4	6.877238645093297E-4	6.927621345789953E-4	1.511481020899626E-4
80-84	0.021143100347350934	4.408486310957242E-4	2.6450917865743456E-4	7.053578097531587E-4	9.295608278532699E-4	1.511481020899626E-4
85-89	0.02117332996776893	4.761165215833822E-4	2.745857187967654E-4	7.20472619962155E-4	9.572713132364296E-4	1.511481020899626E-4
90-94	0.02122623180350041	5.013078719317092E-4	2.8970052900576166E-4	7.63297915554311E-4	9.572713132364296E-4	1.511481020899626E-4
95-99	0.021271576234127402	5.03827006966542E-4	2.8970052900576166E-4	8.06123211146467E-4	9.572713132364296E-4	1.511481020899626E-4
100-104	0.021326997204893724	5.139035471058729E-4	2.9221966404059435E-4	8.111614812161326E-4	9.673478533757607E-4	1.511481020899626E-4
105-109	0.021349669420207218	5.542097076631962E-4	3.0733447424959064E-4	8.287954264599615E-4	9.82462663584757E-4	1.511481020899626E-4
110-114	0.021379899040625205	5.74362787941858E-4	3.249684194934196E-4	8.892546672959465E-4	9.975774737937533E-4	1.511481020899626E-4
115-119	0.021566315033202825	5.945158682205196E-4	3.3504495963275043E-4	9.648287183409279E-4	0.00105047930952524	1.7130118236862428E-4
120-124	0.02188120691255692	6.373411638126757E-4	3.577171749462448E-4	0.0010101731489679168	0.0011084194153263924	1.7633945243828968E-4
125-129	0.02219106052184134	6.499368389868393E-4	3.929850654339027E-4	0.0010655941197342364	0.0011713977911972102	1.7633945243828968E-4
130-134	0.02239762959469762	6.700899192655009E-4	4.0809987564289894E-4	0.0011361299007095521	0.0011965891415455375	1.8893512761245323E-4
135-139	0.022538701156648255	7.103960798228241E-4	4.282529559215606E-4	0.001173916926232043	0.0012444527072073587	1.8893512761245323E-4
140-144	0.02271000233901688	7.179534849273223E-4	4.282529559215606E-4	0.0012142230867893664	0.0012570483823815223	1.8893512761245323E-4
145-149	0.022901456601664167	7.280300250666531E-4	4.4588690116538965E-4	0.001302392813008511	0.001279720597695017	1.8893512761245323E-4
150-154	0.023120621349694608	7.431448352756494E-4	4.786356566182148E-4	0.0013502563786703327	0.0013351415684613364	1.9649253271695135E-4
155-157	0.023364977448073383	7.515419520584251E-4	4.954298901837664E-4	0.0013687300355924392	0.0013477372436355	2.0153080278661678E-4
>>END_MODULE
