##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293877
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4472730
Total Bases	744.4 Mbp
Sequences flagged as poor quality	0
Sequence length	90-172
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.03960690674376	41.0	40.0	41.0	38.0	41.0
2	40.15317155294417	41.0	40.0	41.0	38.0	41.0
3	40.17077556660026	41.0	40.0	41.0	38.0	41.0
4	40.173378674769104	41.0	40.0	41.0	38.0	41.0
5	40.155693502625915	41.0	40.0	41.0	38.0	41.0
6	40.12128029190226	41.0	40.0	41.0	38.0	41.0
7	40.13245713468061	41.0	40.0	41.0	38.0	41.0
8	40.10830834859247	41.0	40.0	41.0	38.0	41.0
9	40.079511394606875	41.0	40.0	41.0	38.0	41.0
10-14	40.01112609077677	41.0	40.0	41.0	38.0	41.0
15-19	39.839516805172686	41.0	40.0	41.0	38.0	41.0
20-24	39.60294688031694	40.4	39.2	41.0	36.8	41.0
25-29	39.42376052209724	40.0	39.0	41.0	36.0	41.0
30-34	39.35125062322116	40.4	38.8	41.0	35.2	41.0
35-39	38.93462954392508	40.0	37.4	41.0	35.0	41.0
40-44	38.28019410963773	39.2	36.0	41.0	35.0	41.0
45-49	37.5165756931449	37.6	35.0	40.4	34.4	41.0
50-54	36.76883040111967	36.4	35.0	39.2	34.0	41.0
55-59	35.93184735049958	35.2	34.8	37.4	33.6	40.0
60-64	35.540939470971864	35.0	35.0	36.4	33.6	39.0
65-69	35.28744628001243	35.0	35.0	36.0	34.0	37.4
70-74	35.08351024989213	35.0	35.0	35.0	33.4	37.0
75-79	35.00239240911032	35.0	35.0	35.0	33.0	36.0
80-84	34.82300281930722	35.0	34.6	35.0	32.6	36.2
85-89	39.966644353672145	41.0	40.0	41.0	38.0	41.0
90-94	39.95111945892285	41.0	40.0	41.0	38.0	41.0
95-99	39.82586425799975	41.0	40.0	41.0	37.4	41.0
100-104	39.67168945601183	41.0	39.8	41.0	37.0	41.0
105-109	39.479469331274274	40.6	39.0	41.0	36.0	41.0
110-114	39.19175574629624	40.0	38.4	41.0	35.0	41.0
115-119	38.981262134322535	40.0	38.0	41.0	35.0	41.0
120-124	38.36917805580437	39.6	37.0	40.6	34.4	41.0
125-129	38.16870345605025	39.4	35.6	41.0	34.0	41.0
130-134	37.78944181282299	38.4	35.0	41.0	34.0	41.0
135-139	37.102404978331705	36.8	35.0	39.6	34.0	41.0
140-144	36.367903280174545	35.6	35.0	38.4	34.0	40.8
145-149	35.70071572892116	35.0	35.0	36.8	34.0	39.0
150-154	35.1924374673141	35.0	35.0	36.0	33.2	37.2
155-159	34.8570570952094	35.0	35.0	35.0	33.0	36.2
160-164	34.657413832253305	35.0	35.0	35.0	33.0	36.0
165-169	34.54971244943529	35.0	35.0	35.0	33.0	35.8
170-172	34.179423441619655	35.0	34.333333333333336	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	44.0
30	396.0
31	2366.0
32	10635.0
33	36337.0
34	99578.0
35	253764.0
36	672858.0
37	1419539.0
38	1585928.0
39	388686.0
40	2596.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.826998276220564	26.9900932987236	27.54852182000702	22.634386605048817
2	22.538024875188086	27.293174414731048	27.452383667245734	22.716417042835136
3	22.50095579210013	27.373103227782586	27.465753577792533	22.660187402324755
4	22.94770755221084	27.320920332772154	27.08904852293789	22.642323592079112
5	22.615382551596007	27.56133278780521	27.165601321787815	22.657683338810973
6	22.557476082839788	27.568218962468112	27.331294310186394	22.543010644505703
7	23.00087418645883	27.269117518830782	27.08819892996	22.641809364750387
8	22.712616232144576	27.384796310083555	27.020701003637598	22.881886454134275
9	22.55879518772651	27.46470276542514	27.239873634223393	22.736628412624952
10-14	22.693473560890105	27.40469914347613	27.25520655170332	22.64662074393044
15-19	22.596204108005622	27.26871955159377	27.398921911226477	22.736154429174128
20-24	22.601829307827657	27.315921148828572	27.243473225524458	22.838776317819317
25-29	22.665615854299276	27.34109145868407	27.2201362478844	22.773156439132254
30-34	22.614045560541324	27.326621548808	27.29535205567964	22.763980834971033
35-39	22.585602081949947	27.36846176719811	27.291180107004	22.75475604384794
40-44	22.725507687698567	27.288801246665905	27.166848882002714	22.818842183632814
45-49	22.77843733022114	27.309303266685	27.159108642819934	22.753150760273925
50-54	22.72949630315266	27.276777270257764	27.16430457461103	22.829421851978545
55-59	22.759388561348437	27.298772785301146	27.154771247090704	22.787067406259712
60-64	22.702018677630885	27.26684597550042	27.216706575178918	22.814428771689773
65-69	22.666510162697058	27.305363838192783	27.225841935462235	22.802284063647928
70-74	22.688742669465853	27.254061836954165	27.21393869068779	22.843256802892192
75-79	22.64974635178068	27.293107341601214	27.220847223060634	22.836299083557467
80-84	22.624938236826278	27.281543934017925	27.201141137515567	22.89237669164023
85-89	21.960122788543014	26.92427220064703	28.69616990070941	22.41943511010054
90-94	22.59312829258096	27.459120096520213	27.297562871810815	22.650188739088012
95-99	22.698018201012353	27.415671544754023	27.186080878418284	22.700229375815344
100-104	22.560311106454332	27.407652600781613	27.378523807262884	22.653512485501174
105-109	22.54796612968043	27.27898719450105	27.36457799935662	22.808468676461903
110-114	22.64643476574616	27.360788745188476	27.221317511598077	22.771458977467287
115-119	22.582962729509095	27.36562372249287	27.281696437638924	22.769717110359107
120-124	22.509934145731144	27.402589775230286	27.307852979503338	22.77962309953523
125-129	22.666857185390707	27.347520710810695	27.21524224028296	22.770379863515636
130-134	22.740536941681153	27.3377004237788	27.174352555546083	22.747410078993965
135-139	22.674656802162037	27.387690038237693	27.234031408424187	22.70362175117608
140-144	22.70807517434917	27.39498053848677	27.204460175060795	22.692484112103266
145-149	22.609093751151736	27.48856856215266	27.282341241123	22.619996445572607
150-154	22.53823043974271	27.49450160811755	27.319554631788968	22.64771332035078
155-159	22.53815431115962	27.58344353548457	27.338299545884166	22.54010260747165
160-164	22.466277797043947	27.60834564869366	27.39776842529037	22.52760812897202
165-169	22.384910230630428	27.726140612508043	27.432951132693923	22.455998024167606
170-172	22.41358966280916	27.72918303060135	27.384630122828323	22.472597183761163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	4.5
14	10.0
15	16.0
16	40.0
17	94.0
18	206.0
19	443.0
20	893.0
21	1719.5
22	3082.5
23	5069.0
24	7814.0
25	11565.0
26	16302.0
27	22324.5
28	29238.5
29	36961.0
30	46048.0
31	56098.5
32	67367.0
33	79242.0
34	91633.5
35	105400.5
36	119428.0
37	133287.5
38	146970.5
39	160123.0
40	171668.5
41	181426.5
42	189359.0
43	194701.5
44	197435.5
45	197320.0
46	194194.0
47	188049.0
48	179806.0
49	169818.0
50	158383.5
51	146128.5
52	133549.5
53	121648.5
54	109957.5
55	98904.0
56	89435.5
57	80544.5
58	72545.5
59	65519.5
60	59193.5
61	53366.0
62	47565.0
63	42419.0
64	37191.5
65	31953.5
66	27289.5
67	22568.0
68	18235.0
69	14373.0
70	11013.0
71	8224.0
72	5987.0
73	4272.5
74	2888.5
75	1867.0
76	1195.5
77	756.0
78	431.0
79	241.0
80	142.0
81	79.0
82	36.0
83	12.5
84	6.0
85	4.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-172	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	461.0
92-93	1136.0
94-95	1723.0
96-97	2239.0
98-99	2799.0
100-101	3314.0
102-103	3967.0
104-105	4353.0
106-107	4824.0
108-109	5297.0
110-111	5649.0
112-113	5976.0
114-115	6403.0
116-117	6634.0
118-119	7213.0
120-121	7516.0
122-123	7898.0
124-125	8312.0
126-127	8993.0
128-129	18675.0
130-131	28392.0
132-133	33891.0
134-135	39329.0
136-137	39113.0
138-139	39185.0
140-141	41931.0
142-143	43954.0
144-145	44997.0
146-147	44389.0
148-149	42790.0
150-151	43473.0
152-153	43296.0
154-155	43371.0
156-157	45207.0
158-159	46388.0
160-161	47088.0
162-163	48731.0
164-165	50844.0
166-167	53001.0
168-169	54484.0
170-171	154202.0
172-173	3331292.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.65752957621432
#Duplication Level	Percentage of total
1	95.46863174462068
2	4.133938434425701
3	0.3035753839899455
4	0.05994996893041537
5	0.016722964881509896
6	0.007397843281809291
7	0.003205705364570991
8	0.0021607726999488077
9	0.001092288375263007
>10	0.0033248934301783406
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2357709944485805E-5	0.0	0.0	2.2357709944485805E-5	0.0	0.0
2	2.2357709944485805E-5	0.0	0.0	2.2357709944485805E-5	0.0	0.0
3	4.471541988897161E-5	2.2357709944485805E-5	0.0	2.2357709944485805E-5	0.0	0.0
4	4.471541988897161E-5	2.2357709944485805E-5	0.0	2.2357709944485805E-5	0.0	0.0
5	4.471541988897161E-5	2.2357709944485805E-5	0.0	2.2357709944485805E-5	0.0	0.0
6	4.471541988897161E-5	2.2357709944485805E-5	0.0	2.2357709944485805E-5	0.0	0.0
7	4.471541988897161E-5	4.471541988897161E-5	0.0	4.471541988897161E-5	0.0	0.0
8	4.471541988897161E-5	4.471541988897161E-5	0.0	4.471541988897161E-5	0.0	0.0
9	4.471541988897161E-5	8.943083977794322E-5	0.0	6.707312983345742E-5	0.0	0.0
10-14	4.471541988897161E-5	8.943083977794322E-5	8.943083977794322E-6	9.390238176684037E-5	0.0	0.0
15-19	4.471541988897161E-5	9.837392375573754E-5	2.2357709944485805E-5	1.296747176780177E-4	0.0	0.0
20-24	5.365850386676594E-5	1.4308934364470915E-4	3.1300793922280126E-5	2.056909314892694E-4	1.7886167955588644E-5	0.0
25-29	8.943083977794322E-5	1.7886167955588644E-4	4.471541988897161E-5	2.682925193338297E-4	6.260158784456025E-5	0.0
30-34	1.4308934364470915E-4	1.833332215447836E-4	4.471541988897161E-5	2.77235603311624E-4	8.048775580014891E-5	0.0
35-39	2.8170714530052115E-4	2.191055574559609E-4	8.943083977794322E-5	3.085363972339042E-4	1.6991859557809213E-4	1.3414625966691484E-5
40-44	4.0243877900074447E-4	2.2357709944485806E-4	9.390238176684037E-5	3.353656491672871E-4	2.682925193338297E-4	2.2357709944485805E-5
45-49	5.499996646343508E-4	2.2357709944485806E-4	1.1178854972242903E-4	3.5772335911177293E-4	2.77235603311624E-4	3.577233591117729E-5
50-54	6.439020464011913E-4	2.50406351378241E-4	1.1178854972242903E-4	3.979672370118474E-4	3.443087331450814E-4	6.707312983345742E-5
55-59	7.154467182235459E-4	3.3089410717838995E-4	1.1178854972242903E-4	4.2479648894523035E-4	3.9349569502295016E-4	6.707312983345742E-5
60-64	7.601621381125174E-4	3.6666644308956724E-4	1.3414625966691484E-4	4.4268265690081897E-4	4.3373957292302466E-4	8.943083977794322E-5
65-69	0.0014532511463915773	3.800810690562587E-4	1.3414625966691484E-4	4.6951190883420194E-4	4.829265348008934E-4	8.943083977794322E-5
70-74	0.014791860899271808	3.9349569502295016E-4	1.3414625966691484E-4	4.6951190883420194E-4	5.813004585566309E-4	8.943083977794322E-5
75-79	0.020305272171582005	4.4268265690081897E-4	1.3414625966691484E-4	5.008127027564821E-4	7.51219054134723E-4	8.943083977794322E-5
80-84	0.020408117637326643	4.7845499281199625E-4	1.5650396961140064E-4	5.186988707120707E-4	0.00102398311545745	8.943083977794322E-5
85-89	0.02061828011080481	5.142273287231736E-4	1.5650396961140064E-4	5.365850386676594E-4	0.001050812367390833	8.943083977794322E-5
90-94	0.02066299553069378	5.589427486121451E-4	1.8780476353368077E-4	5.365850386676594E-4	0.0010642269933575244	8.943083977794322E-5
95-99	0.02069429632461606	6.170727944678082E-4	2.0569093148926944E-4	5.634142906010423E-4	0.0010731700773353187	8.943083977794322E-5
100-104	0.020712182492571653	6.260158784456026E-4	2.2357709944485806E-4	6.260158784456026E-4	0.0010731700773353187	8.943083977794322E-5
105-109	0.020752426370471724	6.260158784456026E-4	2.2357709944485806E-4	6.841459243012657E-4	0.0010731700773353187	8.943083977794322E-5
110-114	0.020801613332349596	6.886174662901628E-4	2.682925193338297E-4	7.512190541347231E-4	0.0010731700773353187	8.943083977794322E-5
115-119	0.020926816508038716	7.109751762346486E-4	3.040648552450069E-4	8.272352679459749E-4	0.001108942413246496	8.943083977794322E-5
120-124	0.021060962767705627	7.333328861791345E-4	3.130079392228013E-4	8.495929778904607E-4	0.0011536578331354675	8.943083977794322E-5
125-129	0.021257710615217106	7.378044281680316E-4	3.2195102320059564E-4	8.943083977794322E-4	0.001189430169046645	1.1178854972242903E-4
130-134	0.021512588508584243	7.556905961236202E-4	3.6219490110067003E-4	9.16666107723918E-4	0.0012252025049578222	1.3414625966691484E-4
135-139	0.02175405177598469	7.825198480570032E-4	4.1585340496743604E-4	9.345522756795067E-4	0.0012609748408689994	1.3414625966691484E-4
140-144	0.0218926695776405	7.825198480570032E-4	4.2479648894523035E-4	9.747961535795811E-4	0.0012878040928023826	1.3414625966691484E-4
145-149	0.022044702005263005	7.825198480570032E-4	4.2479648894523035E-4	0.001082113161313113	0.0013191048867246626	1.3414625966691484E-4
150-154	0.022326409150563522	7.825198480570032E-4	4.382111149119218E-4	0.0011447147491576732	0.001332519512691354	1.3414625966691484E-4
155-159	0.022608116295864046	7.825198480570032E-4	4.471541988897161E-4	0.001162600917113262	0.0013504056806469427	1.38617801655812E-4
160-161	0.022771327578458792	7.825198480570032E-4	4.471541988897161E-4	0.001173779772085505	0.0013638203066136342	1.6768282458364355E-4
>>END_MODULE
