##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293879
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4165813
Total Bases	671.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-175
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.04938651830987	40.0	39.0	41.0	36.0	41.0
2	39.21366585586055	40.0	39.0	41.0	36.0	41.0
3	39.32338105431041	40.0	39.0	41.0	36.0	41.0
4	39.33549993722714	40.0	39.0	41.0	36.0	41.0
5	39.34075629414954	40.0	39.0	41.0	36.0	41.0
6	39.30185728452045	40.0	39.0	41.0	36.0	41.0
7	39.301199069665394	40.0	39.0	41.0	36.0	41.0
8	39.27934235166101	40.0	39.0	41.0	36.0	41.0
9	39.25728087170499	40.0	39.0	41.0	36.0	41.0
10-14	39.144429046623074	40.0	38.4	41.0	36.0	41.0
15-19	38.95079836756955	40.0	38.0	41.0	35.4	41.0
20-24	38.714325679045125	40.0	38.0	40.0	35.0	41.0
25-29	38.54128315409262	40.0	37.6	40.0	34.8	41.0
30-34	38.15755930475035	39.6	36.6	40.0	34.0	41.0
35-39	37.84946909522823	39.0	35.8	40.0	33.6	41.0
40-44	37.305753042683385	38.0	35.0	40.0	33.0	41.0
45-49	36.55065760272965	36.4	34.8	39.6	33.0	40.8
50-54	35.864671409878454	35.4	34.0	38.6	32.4	40.0
55-59	35.265400391232156	35.0	34.0	36.8	32.0	39.4
60-64	34.68845380241504	35.0	33.6	36.0	31.8	38.8
65-69	34.607313914474794	35.0	33.6	35.6	32.0	37.2
70-74	34.47369514666165	35.0	33.0	35.0	32.0	37.0
75-79	34.425024118941494	35.0	33.0	35.0	32.0	37.0
80-84	34.42655918544591	35.0	33.0	35.0	31.6	38.0
85-89	36.622336000199724	38.4	34.8	39.0	31.0	39.6
90-94	38.03615575943469	40.0	36.6	41.0	32.0	41.0
95-99	37.924492872660394	40.0	36.0	41.0	32.0	41.0
100-104	37.77516903478276	40.0	36.0	40.6	32.0	41.0
105-109	37.56803470104437	39.2	35.2	40.0	31.8	41.0
110-114	37.32591553285527	39.0	35.0	40.0	31.0	41.0
115-119	37.03397620618019	38.2	34.6	40.0	31.0	41.0
120-124	36.81335262015509	38.0	34.0	40.0	31.0	41.0
125-129	36.22876412906912	37.0	33.8	39.6	30.2	40.6
130-134	36.05946302071151	36.4	34.0	40.0	30.0	41.0
135-139	35.91066175510406	35.8	34.0	39.4	31.0	41.0
140-144	35.45161913156208	35.0	34.0	38.4	31.0	40.2
145-149	34.93654572943417	35.0	33.2	36.8	30.8	39.4
150-154	34.472860802158756	35.0	33.0	36.0	30.0	38.0
155-159	34.116025621339006	35.0	33.0	35.0	30.0	36.6
160-164	33.875402861281096	35.0	33.0	35.0	30.0	36.0
165-169	33.745470143590396	35.0	33.0	35.0	30.0	35.6
170-174	33.683410861281985	35.0	33.0	35.0	30.0	35.0
175	32.85494776303027	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	88.0
29	1391.0
30	8406.0
31	36876.0
32	115777.0
33	229938.0
34	371259.0
35	621893.0
36	965857.0
37	1083101.0
38	637984.0
39	92948.0
40	294.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.504394700386214	27.355404575289384	27.920288308668678	22.219912415655717
2	21.94961703753865	27.433564588712933	28.359026197287303	22.257792176461113
3	22.009965401711504	27.489159979096517	28.03383157141235	22.46704304777963
4	22.382233672034726	27.714710189823695	27.59190102868276	22.311155109458827
5	22.27361141750722	27.837855419818414	27.755374521131888	22.13315864154248
6	22.09683920041538	27.71350994391731	27.703235838958683	22.486415016708623
7	22.357748655544547	27.803816445913437	27.539450282573895	22.29898461596812
8	22.387874827794718	27.77738703105492	27.66069912403653	22.174039017113824
9	22.206613691013015	27.56028655150867	27.575769723700994	22.65733003377732
10-14	22.32064665408649	27.67471319523944	27.616736516977596	22.38790363369647
15-19	22.258459513185063	27.54437129079006	27.665514510612933	22.531654685411947
20-24	22.273164926030045	27.483605241041786	27.63175399375824	22.611475839169927
25-29	22.336994003331405	27.579586505683285	27.526785287769755	22.556634203215555
30-34	22.27874846998653	27.50563695489932	27.545629148499945	22.669985426614208
35-39	22.340282677114885	27.528019140561515	27.564933903658183	22.566764278665413
40-44	22.450234804106664	27.509213687700335	27.42474038080922	22.61581112738378
45-49	22.65015256325716	27.43367981231995	27.30498464525412	22.611182979168774
50-54	22.566298583253737	27.422704763751998	27.40614617122756	22.604850481766707
55-59	22.550464939256752	27.52603633432418	27.3473005149295	22.57619821148957
60-64	22.43016669255197	27.46696983277934	27.42583500507584	22.677028469592848
65-69	22.41472672921228	27.520299158891675	27.43723734118646	22.627736770709582
70-74	22.373385459212884	27.568405014819437	27.424433117857188	22.633776408110492
75-79	22.326657485585645	27.558471779698223	27.390778222642254	22.724092512073874
80-84	22.274321963083796	27.577267630592157	27.468736594753533	22.679673811570513
85-89	21.647755192083753	27.86186993991329	28.259895487387453	22.2304793806155
90-94	21.648924268969697	27.676555278206006	28.196823458987957	22.477696993836346
95-99	21.951725840072122	27.93252665545718	27.641865547895367	22.47388195657533
100-104	21.971108704867614	27.907862995645267	27.604663720366666	22.51636457912045
105-109	21.963372823028838	27.81883672092187	27.618877219419424	22.598913236629873
110-114	22.034077466448203	27.744847930305887	27.56390288440244	22.657171718843465
115-119	22.03947297992692	27.84728437479539	27.49453852632449	22.6187041189532
120-124	22.04167157208219	27.785357092747027	27.527535508488654	22.645435826682128
125-129	22.163389739912944	27.793880840649354	27.441420596632444	22.60130882280526
130-134	22.417698974880874	27.749342029884144	27.28209162886779	22.550867366367196
135-139	22.31930074630929	27.81648006069744	27.36432648029024	22.499892712703026
140-144	22.281390507033564	27.84436782736716	27.46004744048503	22.41419422511424
145-149	22.209300279515983	27.965913039514156	27.49534406164894	22.329442619320925
150-154	22.121296724685312	27.99458767409582	27.56219322176311	22.321922379455764
155-159	22.058711906716166	28.11332997981167	27.64102387435315	22.186934239119015
160-164	21.927269074098167	28.24566744045568	27.70656511684355	22.120498368602608
165-169	21.86728253225055	28.297212368759777	27.76661134511538	22.068893753874296
170-174	21.712251647216803	28.435784190088615	27.908987359442783	21.942976803251803
175	21.82257955723266	28.527266111663707	27.61419979156513	22.035954539538498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	2.5
14	10.0
15	22.0
16	54.5
17	122.5
18	273.0
19	633.5
20	1339.5
21	2427.5
22	4228.5
23	7054.5
24	10857.5
25	15884.5
26	22159.0
27	29371.5
28	37571.5
29	46860.0
30	56804.5
31	66805.5
32	77672.0
33	88743.0
34	99542.0
35	110988.0
36	122420.0
37	132656.5
38	142322.0
39	151379.5
40	159055.5
41	165657.0
42	169832.5
43	171436.5
44	171582.0
45	168820.5
46	163630.0
47	157236.5
48	149178.0
49	139776.5
50	130007.0
51	120430.0
52	110811.5
53	101818.5
54	93460.0
55	86049.5
56	80064.5
57	74297.5
58	68503.5
59	63715.5
60	58901.5
61	53622.5
62	48498.0
63	43268.0
64	38071.5
65	32743.0
66	27495.5
67	22741.5
68	18294.5
69	14219.0
70	10785.0
71	7977.5
72	5705.5
73	3971.0
74	2679.0
75	1793.0
76	1107.5
77	640.5
78	387.0
79	219.5
80	116.5
81	58.0
82	33.0
83	15.5
84	8.0
85	4.5
86	2.0
87	0.5
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1606.0
92-93	3663.0
94-95	5484.0
96-97	7055.0
98-99	8678.0
100-101	10340.0
102-103	11433.0
104-105	12614.0
106-107	13768.0
108-109	14809.0
110-111	15820.0
112-113	16628.0
114-115	17839.0
116-117	18452.0
118-119	19156.0
120-121	19819.0
122-123	20612.0
124-125	21466.0
126-127	22262.0
128-129	23556.0
130-131	83106.0
132-133	79296.0
134-135	70938.0
136-137	78664.0
138-139	85411.0
140-141	82017.0
142-143	83655.0
144-145	86010.0
146-147	78483.0
148-149	70679.0
150-151	74567.0
152-153	71777.0
154-155	69301.0
156-157	75867.0
158-159	78289.0
160-161	65435.0
162-163	70483.0
164-165	71877.0
166-167	70365.0
168-169	69551.0
170-171	72402.0
172-173	79852.0
174-175	2212728.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.05746631589467
#Duplication Level	Percentage of total
1	96.18520669852792
2	3.623041345518375
3	0.16794838484171193
4	0.014511133577164721
5	0.0020980842871903952
6	0.0021233465426011107
7	0.001074402859437665
8	0.0
9	0.0
>10	0.003996603845594289
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	2.400491812762599E-5	0.0
2	0.0	0.0	0.0	2.400491812762599E-5	4.800983625525198E-5	0.0
3	0.0	0.0	0.0	2.400491812762599E-5	4.800983625525198E-5	0.0
4	0.0	0.0	0.0	2.400491812762599E-5	4.800983625525198E-5	0.0
5	0.0	0.0	0.0	2.400491812762599E-5	4.800983625525198E-5	0.0
6	7.201475438287796E-5	2.400491812762599E-5	0.0	7.201475438287796E-5	4.800983625525198E-5	0.0
7	7.201475438287796E-5	2.400491812762599E-5	0.0	7.201475438287796E-5	4.800983625525198E-5	0.0
8	7.201475438287796E-5	2.400491812762599E-5	0.0	7.201475438287796E-5	4.800983625525198E-5	0.0
9	7.201475438287796E-5	2.400491812762599E-5	0.0	7.201475438287796E-5	4.800983625525198E-5	0.0
10-14	1.4883049239128112E-4	2.400491812762599E-5	4.800983625525197E-6	8.641770525945356E-5	6.241278713182757E-5	0.0
15-19	1.6803442689338193E-4	2.400491812762599E-5	2.8805901753151184E-5	9.601967251050395E-5	9.601967251050395E-5	0.0
20-24	1.6803442689338193E-4	2.400491812762599E-5	4.800983625525198E-5	1.3922852514023073E-4	9.601967251050395E-5	0.0
25-29	1.6803442689338193E-4	3.840786900420158E-5	7.201475438287798E-5	1.6323344326785673E-4	1.2482557426365514E-4	0.0
30-34	1.920393450210079E-4	8.641770525945356E-5	1.2002459063812995E-4	1.6803442689338193E-4	2.1604426314863389E-4	0.0
35-39	3.2646688653571347E-4	1.1522360701260474E-4	1.6803442689338193E-4	1.6803442689338193E-4	2.9286000115703707E-4	0.0
40-44	4.5129246079936866E-4	1.5363147601680631E-4	2.2084524677415908E-4	1.7283541051890712E-4	3.5527278828886464E-4	1.920393450210079E-5
45-49	5.137052479311962E-4	1.6803442689338193E-4	2.400491812762599E-4	2.0644229589758347E-4	3.600737719143898E-4	4.320885262972678E-5
50-54	5.953219695651244E-4	1.920393450210079E-4	3.0726295203361263E-4	2.2564623039968428E-4	3.696757391654402E-4	4.800983625525198E-5
55-59	6.433318058203765E-4	2.0164131227205827E-4	3.1206393565913785E-4	2.544521321528355E-4	4.6089442805041894E-4	4.800983625525198E-5
60-64	7.297495110798301E-4	2.400491812762599E-4	3.2646688653571347E-4	2.8805901753151185E-4	4.993022970546207E-4	6.721377075735277E-5
65-69	8.881819707221615E-4	2.832580339059866E-4	4.0808360816964177E-4	2.9286000115703707E-4	5.473121333098726E-4	8.641770525945356E-5
70-74	0.00556914100560923	2.8805901753151185E-4	4.3208852629726777E-4	3.3606885378676386E-4	5.953219695651244E-4	1.1042262338707954E-4
75-79	0.009640375120054598	2.8805901753151185E-4	4.3208852629726777E-4	3.744767227909655E-4	7.249485274543048E-4	1.2002459063812995E-4
80-84	0.009726792825314048	2.9766098478256224E-4	4.560934444248938E-4	4.5129246079936855E-4	9.121868888497876E-4	1.2002459063812995E-4
85-89	0.009760399710692726	3.1206393565913785E-4	4.7049639530146934E-4	4.800983625525198E-4	9.265898397263631E-4	1.2002459063812995E-4
90-94	0.009794006596071403	3.1206393565913785E-4	4.800983625525198E-4	4.800983625525198E-4	9.601967251050396E-4	1.2002459063812995E-4
95-99	0.009818011514199029	3.1206393565913785E-4	4.800983625525198E-4	4.897003298035702E-4	9.601967251050396E-4	1.2002459063812995E-4
100-104	0.009894827252207432	3.1206393565913785E-4	4.800983625525198E-4	5.425111496843474E-4	9.89002626858191E-4	1.2002459063812995E-4
105-109	0.00996684200659031	3.3606885378676386E-4	4.84899346178045E-4	5.905209859395994E-4	0.0010322114794879176	1.2002459063812995E-4
110-114	0.01003885676097319	3.696757391654402E-4	5.521131169353977E-4	6.001229531906497E-4	0.0010322114794879176	1.2002459063812995E-4
115-119	0.010187687253364469	3.984816409185914E-4	5.713170514374985E-4	6.049239368161749E-4	0.0010466144303644931	1.2002459063812995E-4
120-124	0.010240498073245247	4.224865590462173E-4	5.905209859395994E-4	6.385308221948513E-4	0.0010850222993686945	1.2002459063812995E-4
125-129	0.010451741352768355	4.464914771738434E-4	6.097249204417001E-4	6.481327894459017E-4	0.0011378331192494718	1.2002459063812995E-4
130-134	0.01067738758316804	4.560934444248938E-4	6.433318058203765E-4	6.577347566969522E-4	0.0011522360701260474	1.2002459063812995E-4
135-139	0.010811815124682744	4.752973789269945E-4	6.481327894459017E-4	6.961426257011536E-4	0.001214648857257875	1.2002459063812995E-4
140-144	0.010898232829942197	4.800983625525198E-4	7.057445929522041E-4	6.961426257011536E-4	0.001238653775385501	1.2962655788918034E-4
145-149	0.011013456436954804	4.993022970546207E-4	7.441524619564056E-4	6.961426257011536E-4	0.001277061644389703	1.4402950876575592E-4
150-154	0.011152684962095033	5.185062315567213E-4	7.489534455819308E-4	7.201475438287796E-4	0.0013490763987725808	1.4402950876575592E-4
155-159	0.011263107585482114	5.281081988077717E-4	7.681573800840316E-4	7.201475438287796E-4	0.0014018872186533577	1.4402950876575592E-4
160-164	0.011392734143371294	5.281081988077717E-4	7.77759347335082E-4	7.201475438287796E-4	0.0014402950876575592	1.4402950876575592E-4
>>END_MODULE
