##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293881
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842550
Total Bases	136 Mbp
Sequences flagged as poor quality	0
Sequence length	90-175
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.13398848733013	40.0	39.0	41.0	36.0	41.0
2	39.29143314936799	40.0	39.0	41.0	36.0	41.0
3	39.403837160999345	40.0	39.0	41.0	37.0	41.0
4	39.40948311672898	40.0	39.0	41.0	37.0	41.0
5	39.40942258619666	40.0	39.0	41.0	37.0	41.0
6	39.37281585662572	40.0	39.0	41.0	37.0	41.0
7	39.360637350899054	40.0	39.0	41.0	36.0	41.0
8	39.26677467212628	40.0	39.0	41.0	36.0	41.0
9	39.27174885763456	40.0	39.0	41.0	36.0	41.0
10-14	39.21792582042609	40.0	38.6	41.0	36.0	41.0
15-19	39.00967491543528	40.0	38.0	41.0	35.6	41.0
20-24	38.76489870037386	40.0	38.0	40.8	35.0	41.0
25-29	38.576301465788376	40.0	37.8	40.4	34.8	41.0
30-34	38.223338199513385	39.6	36.6	40.0	34.0	41.0
35-39	37.91963610468222	39.0	35.8	40.0	34.0	41.0
40-44	37.387538068957326	38.2	35.0	40.0	33.0	41.0
45-49	36.66160441516824	36.6	35.0	39.6	33.0	41.0
50-54	35.99022063972465	35.6	34.0	38.8	33.0	40.0
55-59	35.410907364548095	35.0	34.0	37.2	32.2	39.4
60-64	34.83642679959646	35.0	33.8	36.0	31.8	39.0
65-69	34.78063521452733	35.0	34.0	36.0	32.0	37.8
70-74	34.638585959290246	35.0	34.0	35.0	32.0	37.0
75-79	34.57648258263605	35.0	34.0	35.0	32.0	37.8
80-84	34.58219429114	35.0	34.0	35.0	32.0	38.0
85-89	37.00853266868435	38.4	36.4	39.0	32.0	39.8
90-94	38.49406480967691	40.0	38.0	41.0	33.0	41.0
95-99	38.37832560953156	40.0	37.2	41.0	33.0	41.0
100-104	38.21656877568241	40.0	37.0	41.0	33.0	41.0
105-109	38.009454745282206	40.0	36.2	41.0	33.0	41.0
110-114	37.74053252582083	39.0	35.8	40.0	33.0	41.0
115-119	37.46239267412297	38.8	35.0	40.0	32.0	41.0
120-124	37.2115609676877	38.0	35.0	40.0	32.0	41.0
125-129	36.604497912470904	37.8	34.0	39.8	31.8	40.6
130-134	36.5025651331839	36.8	34.0	40.0	31.8	41.0
135-139	36.322710765479556	36.2	34.0	39.6	32.0	41.0
140-144	35.834805233642854	35.2	34.0	38.8	32.0	40.4
145-149	35.28778447712923	35.0	34.0	37.0	32.0	39.4
150-154	34.803972582835016	35.0	34.0	36.0	32.0	38.2
155-159	34.434038925919324	35.0	34.0	35.2	31.8	36.8
160-164	34.1744518780862	35.0	34.0	35.0	31.0	36.0
165-169	34.040654374021244	35.0	34.0	35.0	31.0	35.8
170-174	33.96961738389857	35.0	34.0	35.0	31.0	35.0
175	33.08640259653318	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	9.0
29	119.0
30	851.0
31	4029.0
32	13197.0
33	30954.0
34	63898.0
35	122461.0
36	201539.0
37	235330.0
38	146860.0
39	23216.0
40	87.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.679128835083972	27.339861135837634	27.78209008367456	22.198919945403834
2	21.89911577947896	27.346507625660198	28.40484244258501	22.34953415227583
3	22.087353866239393	27.536169960239747	27.879532371966054	22.496943801554803
4	22.408284374814553	27.632900124621685	27.501513263307814	22.45730223725595
5	22.39950151326331	27.7234585484541	27.611299032698355	22.265740905584238
6	22.204379562043798	27.608213162423596	27.580321642632484	22.607085632900127
7	22.450062310842085	27.72796866654798	27.471485371788024	22.35048365082191
8	22.563764761735207	27.69936502284731	27.52584416355112	22.21102605186636
9	22.308468340157855	27.418906889798826	27.5131446204973	22.75948014954602
10-14	22.438905702925645	27.54951041481218	27.496314758768026	22.515269123494157
15-19	22.353688208414933	27.429375111269362	27.582909026170555	22.634027654145157
20-24	22.33984926710581	27.377532490653373	27.56878523529761	22.713833006943208
25-29	22.426870808854073	27.511554210432614	27.40846240579194	22.65311257492137
30-34	22.326580024924336	27.433220580380986	27.4717464838882	22.76845291080648
35-39	22.419678357367516	27.451640852174947	27.463319684291733	22.665361106165808
40-44	22.546958637469587	27.392059818408406	27.334757581152452	22.72622396296956
45-49	22.742958874844224	27.322319150198805	27.249516349178087	22.685205625778885
50-54	22.664126758055904	27.333119696160463	27.304302415286923	22.698451130496704
55-59	22.651996914129725	27.405827547326567	27.27717049433268	22.665005044211025
60-64	22.52443178446383	27.4215892231915	27.317381757759186	22.736597234585485
65-69	22.539196486855378	27.39524063853777	27.354198563883447	22.7113643107234
70-74	22.465254287579373	27.485799062370187	27.332882321523943	22.716064328526496
75-79	22.43750519257017	27.463058572191564	27.29960239748383	22.799833837754434
80-84	22.368524123197435	27.50367337250015	27.336158091507922	22.791644412794493
85-89	21.75218087947303	27.801768441042075	28.10392261586849	22.342128063616403
90-94	21.75739501488277	27.577172988094496	28.106150873052194	22.559281123970536
95-99	22.052506921782783	27.850175980758614	27.52620652951269	22.571110567945915
100-104	22.065083540567606	27.850968610596023	27.477563595766192	22.606384253070182
105-109	22.07126991791405	27.686793819410916	27.585441815548045	22.656494447126992
110-114	22.092346479169837	27.643108672508394	27.479760637364436	22.784784210957334
115-119	22.12336222754301	27.770545054418715	27.385620174271953	22.720472543766327
120-124	22.159954926115194	27.680657811347636	27.393334242858753	22.766053019678424
125-129	22.247179989943298	27.715391785408478	27.35784542863723	22.679582796010994
130-134	22.485332811231576	27.632712868853698	27.21403342874535	22.667920891169384
135-139	22.429558239451662	27.710671233939838	27.244665671929123	22.615104854679373
140-144	22.342249408357322	27.74991282734709	27.369138037968405	22.538699726327184
145-149	22.30033368636428	27.90536233649813	27.35850706885393	22.435796908283663
150-154	22.21138669434128	27.929643624161294	27.422580044609333	22.436389636888094
155-159	22.1677140562438	27.970613343169948	27.519764640990658	22.3419079595956
160-164	22.065527510995754	28.12090820961004	27.565298490302954	22.248265789091256
165-169	21.90035287796005	28.215926145966346	27.672988455675	22.2107325203986
170-174	21.820417464632964	28.334024210188026	27.801660899009327	22.043897426169682
175	21.942182814588417	28.447366416354836	27.40649087127447	22.203959897782283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	4.5
16	13.5
17	24.5
18	53.5
19	103.0
20	211.5
21	470.5
22	822.0
23	1315.5
24	2005.5
25	2972.0
26	4197.5
27	5598.0
28	7206.5
29	9020.5
30	10904.0
31	12853.5
32	14956.0
33	17262.5
34	19753.0
35	22035.0
36	24168.0
37	26359.5
38	28501.5
39	30467.5
40	32263.0
41	33525.0
42	34418.0
43	34998.5
44	34888.5
45	34226.5
46	33265.0
47	32047.5
48	30541.5
49	28758.0
50	26865.0
51	24896.5
52	22905.5
53	21015.5
54	19296.5
55	17744.0
56	16450.0
57	15336.0
58	14132.0
59	12958.0
60	11976.5
61	11052.5
62	9988.0
63	8938.0
64	7792.5
65	6698.0
66	5714.0
67	4669.5
68	3768.5
69	2959.5
70	2223.5
71	1676.0
72	1187.0
73	827.0
74	563.5
75	361.5
76	232.5
77	154.5
78	92.0
79	43.0
80	26.0
81	13.0
82	4.0
83	3.0
84	2.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	329.0
92-93	750.0
94-95	1162.0
96-97	1488.0
98-99	1819.0
100-101	2136.0
102-103	2374.0
104-105	2601.0
106-107	2920.0
108-109	3134.0
110-111	3271.0
112-113	3270.0
114-115	3676.0
116-117	3757.0
118-119	3930.0
120-121	4019.0
122-123	4140.0
124-125	4403.0
126-127	4524.0
128-129	4783.0
130-131	15473.0
132-133	16609.0
134-135	14436.0
136-137	16245.0
138-139	15656.0
140-141	16971.0
142-143	14309.0
144-145	15085.0
146-147	15331.0
148-149	15479.0
150-151	15289.0
152-153	14588.0
154-155	13760.0
156-157	13932.0
158-159	13084.0
160-161	13298.0
162-163	17524.0
164-165	13154.0
166-167	13228.0
168-169	13875.0
170-171	14658.0
172-173	14638.0
174-175	457442.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.42550617636533
#Duplication Level	Percentage of total
1	98.85386809612149
2	1.1381631805857577
3	0.006771709533257814
4	0.0011970137594863265
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.3737463652008783E-4	0.0	0.0
2	0.0	0.0	0.0	2.3737463652008783E-4	0.0	0.0
3	0.0	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
4	0.0	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
5	0.0	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
6	0.0	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
7	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
8	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
9	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
10-14	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
15-19	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
20-24	1.1868731826004391E-4	0.0	1.1868731826004391E-4	2.3737463652008783E-4	0.0	0.0
25-29	1.1868731826004391E-4	0.0	2.1363717286807904E-4	2.3737463652008783E-4	0.0	0.0
30-34	1.1868731826004391E-4	0.0	2.3737463652008783E-4	2.3737463652008783E-4	0.0	0.0
35-39	2.611121001720966E-4	4.747492730401756E-5	2.3737463652008783E-4	2.3737463652008783E-4	0.0	4.747492730401756E-5
40-44	5.222242003441932E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	0.0	1.1868731826004391E-4
45-49	6.409115186042371E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	0.0	1.1868731826004391E-4
50-54	8.070737641682988E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	7.121239095602635E-5	1.1868731826004391E-4
55-59	8.782861551243249E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	1.661622455640615E-4	1.1868731826004391E-4
60-64	9.494985460803513E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	2.3737463652008783E-4	1.1868731826004391E-4
65-69	9.494985460803513E-4	1.1868731826004391E-4	2.3737463652008783E-4	2.3737463652008783E-4	2.3737463652008783E-4	1.1868731826004391E-4
70-74	0.005388404249005994	1.1868731826004391E-4	2.611121001720966E-4	2.3737463652008783E-4	3.32324491128123E-4	1.1868731826004391E-4
75-79	0.008426799596463117	2.1363717286807904E-4	3.5606195478013177E-4	2.3737463652008783E-4	5.222242003441932E-4	1.1868731826004391E-4
80-84	0.008426799596463117	2.3737463652008783E-4	4.035368820841493E-4	2.3737463652008783E-4	7.121239095602635E-4	1.1868731826004391E-4
85-89	0.008426799596463117	3.32324491128123E-4	5.45961663996202E-4	3.085870274761142E-4	7.121239095602635E-4	1.1868731826004391E-4
90-94	0.008426799596463117	4.035368820841493E-4	5.934365913002195E-4	3.5606195478013177E-4	7.121239095602635E-4	1.1868731826004391E-4
95-99	0.008426799596463117	4.7474927304017565E-4	5.934365913002195E-4	4.5101180938816686E-4	7.8333630051629E-4	1.1868731826004391E-4
100-104	0.008521749451071153	4.7474927304017565E-4	5.934365913002195E-4	4.7474927304017565E-4	9.494985460803513E-4	1.1868731826004391E-4
105-109	0.008592961842027179	4.7474927304017565E-4	5.934365913002195E-4	4.7474927304017565E-4	9.494985460803513E-4	1.1868731826004391E-4
110-114	0.008687911696635214	5.222242003441932E-4	5.934365913002195E-4	4.7474927304017565E-4	9.494985460803513E-4	1.1868731826004391E-4
115-119	0.008830336478547267	5.934365913002195E-4	5.934365913002195E-4	4.7474927304017565E-4	9.494985460803513E-4	1.1868731826004391E-4
120-124	0.008901548869503293	5.934365913002195E-4	5.934365913002195E-4	4.7474927304017565E-4	9.494985460803513E-4	1.1868731826004391E-4
125-129	0.008996498724111329	7.121239095602635E-4	7.121239095602635E-4	5.696991276482107E-4	9.494985460803513E-4	1.1868731826004391E-4
130-134	0.009210135896979407	7.121239095602635E-4	8.308112278203074E-4	5.934365913002195E-4	9.732360097323601E-4	1.1868731826004391E-4
135-139	0.00937629814254347	7.121239095602635E-4	8.308112278203074E-4	5.934365913002195E-4	0.001091923327992404	1.1868731826004391E-4
140-144	0.009471247997151504	7.121239095602635E-4	8.308112278203074E-4	5.934365913002195E-4	0.0012580855735564655	1.1868731826004391E-4
145-149	0.00954246038810753	7.121239095602635E-4	8.308112278203074E-4	7.121239095602635E-4	0.0014954602100765535	1.1868731826004391E-4
150-154	0.009827309951931637	7.121239095602635E-4	8.308112278203074E-4	7.121239095602635E-4	0.0016853599192926234	1.1868731826004391E-4
155-159	0.010017209661147705	7.121239095602635E-4	8.308112278203074E-4	7.121239095602635E-4	0.001780309773900659	1.1868731826004391E-4
160-164	0.010230846834015785	7.121239095602635E-4	8.308112278203074E-4	7.121239095602635E-4	0.001780309773900659	1.661622455640615E-4
>>END_MODULE
