##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293886
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8792592
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.543166906868876	39.0	39.0	39.0	38.0	39.0
2	40.27613939097822	41.0	40.0	41.0	39.0	41.0
3	40.391450211723686	41.0	40.0	41.0	39.0	41.0
4	40.414075166913236	41.0	40.0	41.0	39.0	41.0
5	40.42464906821561	41.0	40.0	41.0	39.0	41.0
6	40.41441806921099	41.0	40.0	41.0	39.0	41.0
7	40.43571065278589	41.0	40.0	41.0	39.0	41.0
8	40.411200246753175	41.0	40.0	41.0	39.0	41.0
9	40.40428817804806	41.0	40.0	41.0	39.0	41.0
10-14	40.366251476242724	41.0	40.0	41.0	39.0	41.0
15-19	40.28404711602676	41.0	40.0	41.0	38.8	41.0
20-24	40.151532858570036	41.0	40.0	41.0	38.0	41.0
25-29	39.951121626023365	41.0	40.0	41.0	37.8	41.0
30-34	39.65260994710093	40.8	39.4	41.0	36.6	41.0
35-39	39.31038851797058	40.0	38.6	41.0	35.4	41.0
40-44	38.711187235800324	40.0	37.2	41.0	35.0	41.0
45-49	37.99845647335849	39.0	35.6	40.8	34.6	41.0
50-54	37.18967212398802	37.4	35.0	40.0	33.8	41.0
55-59	36.350512272149096	36.2	35.0	39.0	33.0	40.6
60-64	35.40714778986674	34.8	33.8	37.2	32.2	39.8
65-69	34.951841936939644	35.0	33.8	36.4	31.2	39.0
70-74	34.83697840181826	35.0	34.0	36.0	31.0	39.4
75-79	34.754495352451244	35.0	33.0	36.0	30.8	40.2
80-84	35.01205958379508	35.0	33.0	38.4	30.0	41.0
85-89	36.044299678638566	35.8	34.0	39.8	30.2	41.0
90-94	39.7787178378706	40.4	39.6	41.0	38.0	41.0
95-99	39.971461971580666	41.0	40.0	41.0	38.0	41.0
100-104	39.80120164511481	41.0	40.0	41.0	37.2	41.0
105-109	39.57567424929131	41.0	39.2	41.0	36.2	41.0
110-114	39.33103022846842	40.4	38.8	41.0	35.2	41.0
115-119	39.01685007282394	40.0	38.0	41.0	35.0	41.0
120-124	38.70256018786334	40.0	37.2	41.0	34.8	41.0
125-129	38.13806688070602	39.6	36.0	40.6	34.0	41.0
130-134	37.768947068087364	39.0	35.0	40.8	34.0	41.0
135-139	37.22522214429431	37.4	35.0	40.2	33.2	41.0
140-144	36.5263999194144	36.2	35.0	39.4	33.0	41.0
145-149	35.800664382421004	35.0	34.4	38.0	32.6	40.2
150-154	35.105130380922546	35.0	34.0	36.6	32.0	39.0
155-159	34.48930191744098	35.0	34.0	35.6	31.0	37.2
160-164	33.97253576930746	35.0	33.0	35.0	31.0	36.2
165-169	33.5502826997879	34.0	33.0	35.0	30.2	35.4
170-174	33.177464526537975	34.0	32.2	35.0	29.6	35.0
175-179	31.94860840253188	32.8	30.2	34.4	27.4	34.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	68.0
31	742.0
32	5459.0
33	28521.0
34	114478.0
35	415411.0
36	1455761.0
37	3269045.0
38	3020323.0
39	479903.0
40	2879.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.8813633112966	27.539069252843756	27.796206169921224	22.783361265938417
2	22.828865481305172	27.52642224272433	28.154143852006325	21.490568423964174
3	21.13424573777562	26.852946207443722	29.20116161423162	22.811646440549044
4	21.692477030663994	26.21421533035992	29.62641732949738	22.466890309478707
5	22.42286461148203	26.719333730030915	28.027309808074797	22.83049185041226
6	22.63358745634962	26.979063739111286	27.65972764345258	22.727621161086514
7	22.52152721290832	27.39064885530911	27.548122328432846	22.53970160334973
8	22.538575655506364	27.41858146039302	27.452257536799156	22.590585347301456
9	22.830958152044357	27.38876090235962	27.248256259360154	22.532024686235868
10-14	22.638377852628665	27.347178169986737	27.416413726464278	22.59803025092032
15-19	22.66731357488213	27.360923832244232	27.404676573187974	22.56708601968566
20-24	22.64507212435195	27.186890964575632	27.487553158386056	22.680483752686353
25-29	22.616982568962598	27.284395773169052	27.383388197700974	22.71523346016738
30-34	22.69537583456619	27.258740084835054	27.380722317150617	22.66516176344814
35-39	22.610204135481325	27.29226830950418	27.369567472253912	22.72796008276058
40-44	22.61018366370235	27.29255263976766	27.39891263008678	22.698351066443205
45-49	22.758490329131615	27.274191728673408	27.24622955324209	22.721088388952882
50-54	22.72625410117972	27.243547750196985	27.305429388739977	22.72476875988332
55-59	22.655128317110588	27.251331575489914	27.29866915239556	22.79487095500394
60-64	22.64187397754837	27.211163670508082	27.381384238003992	22.76557811393955
65-69	22.592848616198726	27.1587490924178	27.38060631040312	22.86779598098035
70-74	22.45627910404577	27.149504946891657	27.52914271468527	22.865073234377302
75-79	22.41616351583242	27.046486405828908	27.54900034028646	22.988349738052214
80-84	22.242665189059156	26.922739051237677	27.761192603955692	23.073403155747474
85-89	21.44752537135807	27.026010077574398	28.151091282297642	23.37537326876989
90-94	22.1035249020494	27.183861204829103	28.19247334093451	22.520140552186984
95-99	22.458028960767948	27.392072396750834	27.533360153118245	22.616538489362977
100-104	22.523704560369787	27.376054632158436	27.4603980532153	22.639842754256478
105-109	22.484949608292403	27.391277951872077	27.48975655835745	22.634015881478074
110-114	22.545435844358813	27.30347739658281	27.438096836763	22.712989922295378
115-119	22.543489426526182	27.375493445578215	27.37311362175701	22.707903506138592
120-124	22.57823009397707	27.370855795876476	27.35730037603366	22.69361373411279
125-129	22.55723761134126	27.41364870003637	27.36441747153016	22.66469621709221
130-134	22.602519948938777	27.40000070882198	27.338072812845994	22.659406529393245
135-139	22.598687120377456	27.471483364094706	27.311784592067628	22.61804492346021
140-144	22.577759513620443	27.509180083745232	27.33302159789665	22.580038804737672
145-149	22.498107252877233	27.602102253484045	27.353667874979493	22.54612261865923
150-154	22.439074075899786	27.688391580365497	27.38743036619637	22.485103977538348
155-159	22.383519371720524	27.801572103657634	27.399453987234068	22.415454537387774
160-164	22.28420132675861	27.92847284684523	27.464560351416946	22.322765474979217
165-169	22.20520997948757	28.10078707815909	27.452585417940433	22.241417524412906
170-174	22.091113187368144	28.283527763112836	27.543230309599476	22.082128739919543
175-179	21.939735082176284	28.457528761549927	27.52018433528126	22.08255182099253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	7.5
11	15.5
12	32.5
13	60.5
14	128.0
15	259.0
16	469.0
17	844.5
18	1545.0
19	2670.0
20	4533.5
21	7491.5
22	11756.0
23	17538.5
24	25042.5
25	34376.0
26	45426.0
27	58515.5
28	72998.5
29	88841.0
30	106375.0
31	124391.0
32	143379.5
33	163606.5
34	184460.0
35	206032.0
36	228208.0
37	250630.5
38	273033.5
39	294494.0
40	314408.0
41	332611.5
42	348521.5
43	361106.5
44	369025.0
45	371638.0
46	369093.0
47	362165.5
48	350387.5
49	333945.5
50	313722.5
51	290552.0
52	266392.5
53	242923.0
54	219229.0
55	196545.5
56	176851.0
57	158776.0
58	143098.5
59	129851.0
60	118039.5
61	106836.0
62	96023.5
63	85912.5
64	75619.0
65	65613.5
66	55712.5
67	46112.0
68	37470.0
69	29700.0
70	23015.0
71	17263.5
72	12532.5
73	8891.5
74	6104.0
75	4058.0
76	2664.5
77	1637.5
78	962.0
79	564.5
80	307.0
81	159.0
82	86.0
83	42.0
84	17.0
85	7.0
86	4.0
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1012.0
92-93	2251.0
94-95	3597.0
96-97	4969.0
98-99	6551.0
100-101	8151.0
102-103	10101.0
104-105	12067.0
106-107	14131.0
108-109	17060.0
110-111	20607.0
112-113	22161.0
114-115	24694.0
116-117	27898.0
118-119	31169.0
120-121	35063.0
122-123	39273.0
124-125	44914.0
126-127	49826.0
128-129	55701.0
130-131	62578.0
132-133	76969.0
134-135	107024.0
136-137	116949.0
138-139	121426.0
140-141	128075.0
142-143	130259.0
144-145	139494.0
146-147	153985.0
148-149	169519.0
150-151	171257.0
152-153	190866.0
154-155	195145.0
156-157	208743.0
158-159	231908.0
160-161	253265.0
162-163	275331.0
164-165	303203.0
166-167	340895.0
168-169	382474.0
170-171	403506.0
172-173	447344.0
174-175	519000.0
176-177	1548409.0
178-179	1683772.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.56180897740394
#Duplication Level	Percentage of total
1	91.54708280624601
2	7.342663301004531
3	0.866341434811317
4	0.1606332608601739
5	0.04089815899737269
6	0.021593532568041
7	0.010340946469092442
8	0.005199737725505056
9	0.003137466437794594
>10	0.002109354880171924
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.274642107810757E-5	1.1373210539053786E-5	1.1373210539053786E-5	0.0
2	2.274642107810757E-5	0.0	2.274642107810757E-5	3.4119631617161354E-5	2.274642107810757E-5	0.0
3	2.274642107810757E-5	1.1373210539053786E-5	3.4119631617161354E-5	3.4119631617161354E-5	2.274642107810757E-5	0.0
4	2.274642107810757E-5	1.1373210539053786E-5	4.549284215621514E-5	3.4119631617161354E-5	2.274642107810757E-5	0.0
5	3.4119631617161354E-5	2.274642107810757E-5	4.549284215621514E-5	3.4119631617161354E-5	2.274642107810757E-5	0.0
6	3.4119631617161354E-5	3.4119631617161354E-5	4.549284215621514E-5	6.823926323432271E-5	2.274642107810757E-5	0.0
7	3.4119631617161354E-5	3.4119631617161354E-5	4.549284215621514E-5	1.0235889485148406E-4	3.4119631617161354E-5	0.0
8	3.4119631617161354E-5	3.4119631617161354E-5	4.549284215621514E-5	1.0235889485148406E-4	3.4119631617161354E-5	0.0
9	6.823926323432271E-5	3.4119631617161354E-5	5.6866052695268925E-5	1.1373210539053785E-4	3.4119631617161354E-5	0.0
10-14	8.871104220461953E-5	5.23167684796474E-5	6.596462112651197E-5	1.4785173700769923E-4	6.823926323432271E-5	0.0
15-19	1.0690817906710557E-4	1.0690817906710557E-4	7.96124737733765E-5	1.8652065284048206E-4	8.643640009680879E-5	2.2746421078107572E-6
20-24	1.1828138960615936E-4	1.2965460014521314E-4	9.780961063586257E-5	2.1609100024202189E-4	9.098568431243029E-5	1.1373210539053786E-5
25-29	1.2510531592959163E-4	1.5695030543894223E-4	1.160067474983486E-4	2.593092002904263E-4	9.098568431243029E-5	1.1373210539053786E-5
30-34	1.5240102122332072E-4	1.8879529494829285E-4	1.478517370076992E-4	2.9342883190758767E-4	1.0918282117491633E-4	1.3647852646864541E-5
35-39	2.0926707391858966E-4	2.1381635813421117E-4	1.8197136862486057E-4	3.5939345303409966E-4	1.2965460014521316E-4	2.274642107810757E-5
40-44	2.547599160748048E-4	2.2746421078107573E-4	2.160910002420219E-4	4.0716093729812556E-4	1.7514744230142832E-4	3.18449895093506E-5
45-49	2.866049055841554E-4	2.6158384239823707E-4	2.4111206342794026E-4	4.5265377945434066E-4	2.3428813710450798E-4	4.321820004840438E-5
50-54	3.616680951419104E-4	3.116259687700737E-4	2.9115418979977687E-4	4.844987689636912E-4	3.0480204244664143E-4	5.686605269526893E-5
55-59	3.957877267590718E-4	3.343723898481813E-4	3.229991793091275E-4	5.117944742574203E-4	3.84414516220018E-4	5.686605269526893E-5
60-64	4.344566425918546E-4	3.6394273724972114E-4	3.5029488460285664E-4	5.459141058745817E-4	4.367312846996653E-4	5.686605269526893E-5
65-69	4.7312555842463744E-4	4.0261165308250397E-4	3.9123844254345015E-4	5.572873164136355E-4	5.117944742574203E-4	5.686605269526893E-5
70-74	0.0014375738121363984	4.0943557940593625E-4	4.390059268074761E-4	5.845830217073645E-4	6.118787270010937E-4	5.686605269526893E-5
75-79	0.016277338923493777	4.253580741606116E-4	4.435552110230976E-4	5.959562322464184E-4	7.2106154817601E-4	5.686605269526893E-5
80-84	0.020424011486032786	4.481044952387192E-4	4.6857627420901596E-4	6.82392632343227E-4	8.871104220461952E-4	5.686605269526893E-5
85-89	0.020535468949315513	4.6857627420901596E-4	4.7767484264025893E-4	7.688290324400359E-4	0.001085004285425731	5.686605269526893E-5
90-94	0.020546842159854566	4.7767484264025893E-4	4.95871979502745E-4	8.165965167040619E-4	0.0010986521380725958	5.686605269526893E-5
95-99	0.020562764654609243	4.7767484264025893E-4	5.163437584730418E-4	8.7118792729152E-4	0.0011259478433663247	5.686605269526893E-5
100-104	0.02060143357044203	5.026959058261772E-4	5.436394637667709E-4	9.257793378789781E-4	0.0011373210539053786	5.686605269526893E-5
105-109	0.02064237712838262	5.231676847964741E-4	5.89132305922986E-4	9.84920032682058E-4	0.0011441449802288106	5.686605269526893E-5
110-114	0.020712891033724756	5.459141058745817E-4	5.959562322464184E-4	9.894693168976793E-4	0.001160067474983486	5.686605269526893E-5
115-119	0.020735637454802863	5.936815901386076E-4	6.346251480792012E-4	0.001030412874838273	0.0011941871066006473	6.36899790187012E-5
120-124	0.020828897781223103	6.118787270010938E-4	6.687447796963626E-4	0.0010736310748866774	0.001244229232972484	6.823926323432271E-5
125-129	0.02100859450774015	6.209772954323367E-4	6.846672744510379E-4	0.0011213985591507034	0.0013329402751771034	6.823926323432271E-5
130-134	0.021256530497491526	6.323505059713904E-4	7.074136955291455E-4	0.001150968906552243	0.0013761584752255082	6.823926323432271E-5
135-139	0.02165459286635841	6.482730007260657E-4	7.392586850384961E-4	0.0012169335276787552	0.0014307498858129662	6.823926323432271E-5
140-144	0.022066303087872153	6.596462112651195E-4	7.574558219009821E-4	0.0012578770856193485	0.0014671441595379383	6.823926323432271E-5
145-149	0.022628139688501407	6.710194218041733E-4	7.620051061166036E-4	0.0013056445698833743	0.00147169344375356	6.823926323432271E-5
150-154	0.023189976289130672	6.914912007744703E-4	7.620051061166036E-4	0.001355686696255211	0.0015103623595863426	6.823926323432271E-5
155-159	0.02424768486926267	6.960404849900917E-4	7.620051061166036E-4	0.0014080034647348586	0.0015194609280175856	6.823926323432271E-5
160-164	0.0259673143027676	7.210615481760101E-4	7.620051061166036E-4	0.0014489470226754521	0.001535383422772261	6.823926323432271E-5
165-168	0.0281657558999667	7.421019876732595E-4	7.620051061166036E-4	0.001478517370076992	0.0015439133306765513	6.823926323432271E-5
>>END_MODULE
