##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293887
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9474094
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.337069064334806	39.0	39.0	39.0	37.0	39.0
2	40.16709851094996	41.0	40.0	41.0	38.0	41.0
3	40.219182858012594	41.0	40.0	41.0	38.0	41.0
4	40.233657909663975	41.0	40.0	41.0	38.0	41.0
5	40.24420118694199	41.0	40.0	41.0	38.0	41.0
6	40.247273987359634	41.0	40.0	41.0	38.0	41.0
7	40.27628214370683	41.0	40.0	41.0	39.0	41.0
8	40.2366495413704	41.0	40.0	41.0	39.0	41.0
9	40.25023215940226	41.0	40.0	41.0	39.0	41.0
10-14	40.19359313935454	41.0	40.0	41.0	38.0	41.0
15-19	40.107155766028924	41.0	40.0	41.0	38.0	41.0
20-24	39.97980026375082	41.0	40.0	41.0	38.0	41.0
25-29	39.8022234738224	41.0	40.0	41.0	37.4	41.0
30-34	39.53520085403417	40.4	39.0	41.0	36.4	41.0
35-39	39.15223477833343	40.0	38.2	41.0	35.0	41.0
40-44	38.72192659266416	40.0	37.2	41.0	35.0	41.0
45-49	38.25700412092175	39.2	35.8	41.0	35.0	41.0
50-54	37.73011118530172	38.0	35.0	40.8	35.0	41.0
55-59	37.00861876607937	36.6	35.0	39.4	35.0	41.0
60-64	36.16572822688903	35.4	34.8	38.0	33.6	40.2
65-69	35.70690668680298	35.0	35.0	36.6	33.8	39.0
70-74	35.42866218131253	35.0	35.0	36.0	34.0	37.6
75-79	35.20178469835743	35.0	35.0	35.2	34.0	37.0
80-84	35.10533062053215	35.0	35.0	35.0	34.0	36.0
85-89	34.996993928918165	35.0	34.8	35.0	33.8	36.0
90-94	39.37832500254691	40.2	39.6	40.2	37.6	40.2
95-99	40.007842738050996	41.0	40.0	41.0	38.0	41.0
100-104	39.91255725394032	41.0	40.0	41.0	38.0	41.0
105-109	39.80227788905797	41.0	40.0	41.0	37.0	41.0
110-114	39.63835990591722	41.0	39.6	41.0	36.6	41.0
115-119	39.429749191052245	40.4	39.0	41.0	35.6	41.0
120-124	39.1405238918558	40.0	38.2	41.0	35.0	41.0
125-129	38.477700314130495	39.6	37.2	40.6	34.6	40.8
130-134	38.178079471027765	39.4	36.0	40.6	34.6	40.8
135-139	38.172873771554464	39.0	35.4	41.0	35.0	41.0
140-144	37.62708276718109	38.0	35.0	40.8	35.0	41.0
145-149	36.90192648652539	36.4	35.0	39.2	34.2	41.0
150-154	36.197170250197004	35.2	35.0	37.6	34.0	40.2
155-159	35.59292592475383	35.0	35.0	36.4	34.0	38.6
160-164	35.1586684388182	35.0	35.0	35.8	34.0	37.0
165-169	34.89429932939084	35.0	35.0	35.0	34.0	36.0
170-174	34.7506131362096	35.0	35.0	35.0	34.0	36.0
175-179	34.590431077896035	35.0	34.8	35.0	33.6	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	47.0
30	481.0
31	3452.0
32	16649.0
33	60547.0
34	166419.0
35	413133.0
36	1164250.0
37	2852822.0
38	3673746.0
39	1107072.0
40	15473.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.072527462784304	27.52998861949227	27.665136106945955	22.732347810777473
2	22.774842639306726	27.540163734917556	28.161637408284108	21.523356217491614
3	21.128743286693165	26.914330805668595	29.170177116672054	22.786748790966186
4	21.72908565188397	26.217208737848708	29.575408477053323	22.478297133214003
5	22.441280401059984	26.757703691772534	28.00598136349502	22.79503454367246
6	22.601422362919347	26.99195300363285	27.682457024386714	22.724167609061087
7	22.47957430019166	27.429704623998873	27.503326439446347	22.58739463636312
8	22.511144601267414	27.46336483467443	27.452503637814868	22.572986926243292
9	22.790738618383983	27.460884386412044	27.234044754041918	22.51433224116206
10-14	22.61508488305056	27.366496469213835	27.4132471136554	22.6051715340802
15-19	22.624037718012932	27.398178654338874	27.409628825722017	22.56815480192618
20-24	22.60476832929882	27.228583545825067	27.467690314240073	22.698957810636035
25-29	22.583149375549787	27.308238655854588	27.37421646861431	22.734395499981318
30-34	22.63634496343397	27.296353614393105	27.36634869782799	22.700952724344937
35-39	22.562366385640676	27.338525456893294	27.359530103881173	22.73957805358486
40-44	22.593446930123346	27.289378804981247	27.37810707810161	22.7390671867938
45-49	22.748974202704765	27.275245527435132	27.224967368911475	22.750812900948628
50-54	22.714754571782798	27.25746862971805	27.29532343673179	22.732453361767362
55-59	22.67949631912033	27.28630727117548	27.27843950038917	22.75575690931502
60-64	22.684879419604663	27.291734703075566	27.314103068852813	22.709282808466963
65-69	22.67023527526748	27.28494777442571	27.275629733038325	22.76918721726848
70-74	22.600970604682622	27.328675438516864	27.3375691649249	22.732784791875613
75-79	22.667469839332394	27.30857430800243	27.253075597518876	22.7708802551463
80-84	22.613843603409467	27.32011736425668	27.303887844051367	22.76215118828249
85-89	22.59645091129558	27.336940081025162	27.266482684254555	22.8001263234247
90-94	22.001032315119932	27.189946446541907	28.500542864829004	22.308478373509157
95-99	22.44744736655576	27.33200676866996	27.575410996838663	22.645134867935617
100-104	22.493212750087473	27.46443277967764	27.443225929008136	22.59912854122675
105-109	22.504900458178483	27.498917943065816	27.4327061874162	22.5634754113395
110-114	22.519958088989732	27.318111463653207	27.469706828440856	22.69222361891621
115-119	22.48788211033987	27.39254498035823	27.38224410653729	22.73732880276461
120-124	22.54187279247667	27.396545964158992	27.35286103641041	22.708720206953934
125-129	22.47778239068969	27.41489129391081	27.360932416923685	22.746393898475816
130-134	22.586729888818464	27.364558233483283	27.322629538401955	22.726082339296298
135-139	22.705223631954965	27.36798022764101	27.24296573538477	22.683830405019258
140-144	22.650075404106378	27.389626537405075	27.32929164256996	22.631006415918588
145-149	22.595308075706928	27.44797900574836	27.31494706978851	22.641765848756208
150-154	22.586538283269167	27.492238065080844	27.352977863652306	22.568245787997686
155-159	22.5369744692559	27.50969237132841	27.35817179301632	22.59516136639937
160-164	22.460032539771305	27.57400336898965	27.440544983906772	22.52541910733227
165-169	22.48508925252947	27.623011718512817	27.391486651114132	22.500412377843585
170-174	22.39806981978009	27.670306175513243	27.46755778742514	22.464066217281523
175-179	22.351619000045325	27.73768625048323	27.453898881659804	22.456795867811643
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	7.0
11	13.5
12	30.5
13	69.5
14	136.0
15	269.0
16	493.0
17	868.5
18	1549.5
19	2746.5
20	4736.5
21	7900.0
22	12361.0
23	18516.0
24	26889.5
25	36754.0
26	48734.0
27	62913.5
28	78625.5
29	96005.5
30	114476.5
31	133756.0
32	154005.0
33	175279.5
34	197456.0
35	221457.0
36	245449.5
37	268602.5
38	292120.5
39	315597.0
40	337524.0
41	357589.5
42	374796.0
43	388167.5
44	397535.5
45	400972.0
46	398601.0
47	391540.5
48	379330.5
49	361822.5
50	340489.5
51	315944.0
52	289504.0
53	262894.0
54	236937.0
55	212865.0
56	191557.5
57	172509.0
58	155466.5
59	140602.5
60	127686.5
61	115856.0
62	104188.0
63	92633.5
64	81412.5
65	70447.5
66	59615.0
67	49121.5
68	39485.5
69	31047.0
70	23891.0
71	17715.5
72	12606.0
73	8808.0
74	5957.5
75	3863.0
76	2456.0
77	1514.5
78	866.0
79	486.0
80	265.0
81	146.5
82	74.0
83	32.0
84	20.0
85	10.5
86	3.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	802.0
92-93	1681.0
94-95	3340.0
96-97	3339.0
98-99	5068.0
100-101	5046.0
102-103	6159.0
104-105	6956.0
106-107	6522.0
108-109	6920.0
110-111	8140.0
112-113	7808.0
114-115	8486.0
116-117	9339.0
118-119	8705.0
120-121	9420.0
122-123	10140.0
124-125	10718.0
126-127	10551.0
128-129	12100.0
130-131	12546.0
132-133	12931.0
134-135	75146.0
136-137	95116.0
138-139	96608.0
140-141	83652.0
142-143	80638.0
144-145	87471.0
146-147	76013.0
148-149	77553.0
150-151	68551.0
152-153	73077.0
154-155	69946.0
156-157	74570.0
158-159	70087.0
160-161	72482.0
162-163	68541.0
164-165	73794.0
166-167	84702.0
168-169	82951.0
170-171	82838.0
172-173	89007.0
174-175	95369.0
176-177	91150.0
178-179	7538115.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.30779614825578
#Duplication Level	Percentage of total
1	91.07982667458555
2	7.706413622080353
3	0.9434103442741343
4	0.18277592257500277
5	0.04797698751027111
6	0.011287879260150116
7	0.010315734103496243
8	0.003110984013356611
9	0.0020848692419530163
>10	0.012796982355724702
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1110197977769696E-5	0.0	0.0	0.0	1.0555098988884848E-5	0.0
2	2.1110197977769696E-5	0.0	0.0	1.0555098988884848E-5	1.0555098988884848E-5	0.0
3	2.1110197977769696E-5	2.1110197977769696E-5	0.0	1.0555098988884848E-5	1.0555098988884848E-5	0.0
4	2.1110197977769696E-5	2.1110197977769696E-5	1.0555098988884848E-5	1.0555098988884848E-5	1.0555098988884848E-5	0.0
5	2.1110197977769696E-5	2.1110197977769696E-5	2.1110197977769696E-5	2.1110197977769696E-5	1.0555098988884848E-5	0.0
6	2.1110197977769696E-5	3.1665296966654544E-5	4.222039595553939E-5	4.222039595553939E-5	2.1110197977769696E-5	0.0
7	3.1665296966654544E-5	3.1665296966654544E-5	4.222039595553939E-5	5.277549494442424E-5	2.1110197977769696E-5	0.0
8	4.222039595553939E-5	3.1665296966654544E-5	5.277549494442424E-5	5.277549494442424E-5	2.1110197977769696E-5	0.0
9	5.277549494442424E-5	3.1665296966654544E-5	5.277549494442424E-5	5.277549494442424E-5	2.1110197977769696E-5	0.0
10-14	7.177467312441697E-5	5.699753453997818E-5	6.544161373108607E-5	6.755263352886303E-5	2.1110197977769696E-5	0.0
15-19	1.0343997009107152E-4	7.388569292219394E-5	1.055509898888485E-4	1.1610608887773331E-4	3.3776316764431515E-5	0.0
20-24	1.1610608887773331E-4	9.499589089996363E-5	1.3510526705772606E-4	1.5621546503549576E-4	6.121957413553211E-5	0.0
25-29	1.2666118786661818E-4	1.1188404928217941E-4	1.5832648483327273E-4	2.132129995754739E-4	7.388569292219394E-5	0.0
30-34	1.646595442266036E-4	1.2666118786661818E-4	1.7521464321548845E-4	3.018758310821066E-4	9.710691069774058E-5	0.0
35-39	3.1031991027321453E-4	1.4354934624883394E-4	2.0054688078881207E-4	3.863166229931854E-4	1.2666118786661818E-4	0.0
40-44	4.939786326798108E-4	1.7732566301326542E-4	2.06879940182143E-4	4.243149793531709E-4	1.4354934624883392E-4	6.333059393330909E-6
45-49	7.071916322552848E-4	1.9632484119325817E-4	2.3221217775546663E-4	4.3909211793760963E-4	2.1532401937325092E-4	2.3221217775546667E-5
50-54	7.705222261885938E-4	2.1110197977769696E-4	2.4276727674435152E-4	4.770904742975951E-4	2.638774747221212E-4	3.588733656220848E-5
55-59	8.422968993130109E-4	2.406562569465745E-4	2.680995143176751E-4	5.425320880286812E-4	3.2720806865543025E-4	6.121957413553212E-5
60-64	9.119605526396509E-4	2.6176645492434426E-4	2.8498767269989085E-4	5.868635037819975E-4	3.9687172198207024E-4	9.499589089996363E-5
65-69	9.83735225764068E-4	2.9554277168877574E-4	2.934317518909988E-4	6.164177809508751E-4	4.728684347020411E-4	1.034399700910715E-4
70-74	0.0018956957784037186	3.208750092620993E-4	3.229860290598763E-4	6.565271571086376E-4	5.488651474220121E-4	1.0555098988884848E-4
75-79	0.017257586846826725	3.483182666331999E-4	3.715394844087466E-4	6.966365332663999E-4	6.016406423664363E-4	1.1610608887773331E-4
80-84	0.024000184080926364	3.9687172198207024E-4	3.7998356359985453E-4	7.409679490197163E-4	6.607491967041915E-4	1.1610608887773331E-4
85-89	0.024065625694657453	4.116488605665091E-4	3.8420560319540843E-4	7.747442657841478E-4	8.042985429530254E-4	1.1610608887773331E-4
90-94	0.024069847734253007	4.116488605665091E-4	4.243149793531709E-4	8.338528201219028E-4	8.359638399196799E-4	1.1610608887773331E-4
95-99	0.024118401189601877	4.3064803874650183E-4	4.4964721692649447E-4	8.718511764818883E-4	8.697401566841115E-4	1.1610608887773331E-4
100-104	0.024223952179490728	4.4964721692649447E-4	4.707574149042642E-4	9.309597308196435E-4	8.845172952685502E-4	1.1610608887773331E-4
105-109	0.024329503169379575	4.686463951064872E-4	4.897565930842569E-4	9.731801267751829E-4	8.887393348641042E-4	1.1610608887773331E-4
110-114	0.024420277020683983	4.8553455348870303E-4	5.235329098486884E-4	0.0010576209186862616	8.97183414055212E-4	1.1610608887773331E-4
115-119	0.024506828832392837	4.8553455348870303E-4	5.48865147422012E-4	0.0011188404928217939	9.225156516285357E-4	1.1610608887773331E-4
120-124	0.02458915860450614	4.876455732864799E-4	5.699753453997817E-4	0.001173727007563995	9.816242059662908E-4	1.1610608887773331E-4
125-129	0.024793927524890506	5.066447514664727E-4	5.931965631753285E-4	0.001239168621295081	0.0010555098988884847	1.2455016806884123E-4
130-134	0.02499447440567932	5.193108702531346E-4	6.501940977153065E-4	0.0013003881954306133	0.001112507433428463	1.2877220766439515E-4
135-139	0.02526046290019922	5.298659692420193E-4	6.670822560975224E-4	0.0013447196111839295	0.0011462837501928945	1.37216286855503E-4
140-144	0.025537006493708	5.467541276242351E-4	6.818593946819611E-4	0.0014017171457239076	0.0011948372055417646	1.4143832645105697E-4
145-149	0.026123869997489994	5.910855433775515E-4	7.093026520530617E-4	0.0014608257000616627	0.0012645008588684048	1.4988240564216482E-4
150-154	0.02679728531298085	6.2697287993976E-4	7.325238698286085E-4	0.0014819358980394325	0.0012961661558350591	1.583264848332727E-4
155-159	0.027008387292758552	6.459720581197526E-4	7.494120282108242E-4	0.0015220452741971952	0.0013447196111839295	1.6888158382215757E-4
160-164	0.02711182726284962	6.839704144797381E-4	7.494120282108242E-4	0.0015558215909616264	0.0013763849081505843	1.794366828110424E-4
165-168	0.02729812476000344	6.86081434277515E-4	7.573283524524878E-4	0.00163340156852993	0.0014117444897633483	2.0318565553603332E-4
>>END_MODULE
