##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293888
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9421437
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.324064577410006	39.0	39.0	39.0	37.0	39.0
2	40.135370538485795	41.0	40.0	41.0	38.0	41.0
3	40.18775872512866	41.0	40.0	41.0	38.0	41.0
4	40.212658111496154	41.0	40.0	41.0	38.0	41.0
5	40.22317317411346	41.0	40.0	41.0	38.0	41.0
6	40.21289066625399	41.0	40.0	41.0	38.0	41.0
7	40.23937325059861	41.0	40.0	41.0	39.0	41.0
8	40.20558424367748	41.0	40.0	41.0	38.0	41.0
9	40.21354619258187	41.0	40.0	41.0	38.0	41.0
10-14	40.161732695341485	41.0	40.0	41.0	38.0	41.0
15-19	40.06641067599348	41.0	40.0	41.0	38.0	41.0
20-24	39.93681331202448	41.0	40.0	41.0	38.0	41.0
25-29	39.75334822065891	41.0	39.8	41.0	37.2	41.0
30-34	39.48297296898552	40.2	39.0	41.0	36.0	41.0
35-39	39.09566319872435	40.0	38.0	41.0	35.0	41.0
40-44	38.69143751637887	40.0	37.0	41.0	35.0	41.0
45-49	38.255245563919814	39.2	35.8	41.0	35.0	41.0
50-54	37.729140873096114	38.0	35.0	40.8	35.0	41.0
55-59	37.016140955992164	36.6	35.0	39.4	34.8	41.0
60-64	36.16514236628659	35.4	34.8	38.0	33.6	40.4
65-69	35.70534976776897	35.0	35.0	36.6	33.8	39.0
70-74	35.43215861869054	35.0	35.0	36.0	34.0	37.6
75-79	35.211050373738104	35.0	35.0	35.4	34.0	37.0
80-84	35.1123372156498	35.0	35.0	35.0	34.0	36.0
85-89	34.99245537596865	35.0	34.8	35.0	33.8	36.0
90-94	39.348456722337104	40.2	39.6	40.2	37.6	40.2
95-99	39.98853889035287	41.0	40.0	41.0	38.0	41.0
100-104	39.890238088098215	41.0	40.0	41.0	37.8	41.0
105-109	39.77431886318425	41.0	40.0	41.0	37.0	41.0
110-114	39.615057602108976	41.0	39.4	41.0	36.4	41.0
115-119	39.40001363346111	40.2	39.0	41.0	35.4	41.0
120-124	39.11152658798396	40.0	38.0	41.0	35.0	41.0
125-129	38.45393816036303	39.6	37.0	40.6	34.6	40.8
130-134	38.15607758400667	39.4	36.0	40.6	34.6	40.8
135-139	38.151974052829395	39.0	35.2	41.0	35.0	41.0
140-144	37.61387493264255	37.8	35.0	40.8	35.0	41.0
145-149	36.89162750743719	36.4	35.0	39.2	34.0	41.0
150-154	36.18896550912841	35.2	35.0	37.6	34.0	40.2
155-159	35.588689783960476	35.0	35.0	36.4	34.0	38.6
160-164	35.150663700206074	35.0	35.0	35.8	34.0	37.0
165-169	34.88884913598233	35.0	35.0	35.0	34.0	36.0
170-174	34.74778210061344	35.0	35.0	35.0	34.0	36.0
175-179	34.59315473212746	35.0	34.8	35.0	33.6	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	5.0
29	55.0
30	606.0
31	3902.0
32	18245.0
33	63355.0
34	169893.0
35	423633.0
36	1175962.0
37	2847021.0
38	3628893.0
39	1075270.0
40	14597.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.07650488985916	27.567418855531272	27.645336905612165	22.710739348997397
2	22.744364792759324	27.57612241105046	28.18443725728888	21.495075538901336
3	21.10889241205986	26.977052439028142	29.190791171240647	22.723263977671344
4	21.64970163256412	26.286276711291496	29.589360943558823	22.474660712585564
5	22.388527355221928	26.80491309340603	28.028908965797893	22.777650585574154
6	22.561377845014512	27.033869674021066	27.70777960941627	22.69697287154815
7	22.487153498983222	27.45679878770086	27.53006786544346	22.525979847872463
8	22.512966970962072	27.46525821910182	27.477103545881587	22.544671264054518
9	22.782671051135832	27.471679744820243	27.27480956461313	22.4708396394308
10-14	22.584707619442767	27.38985783166623	27.4306329278644	22.5948016210266
15-19	22.612159907241328	27.420035818315192	27.410525591796663	22.55727868264682
20-24	22.596801315977594	27.241111945024947	27.482213169816877	22.679873569180582
25-29	22.58228972926317	27.3472847082669	27.370703641068765	22.69972192140116
30-34	22.61580054083045	27.32878222292417	27.374210537097472	22.68120669914791
35-39	22.533579537813605	27.373505761382262	27.36991819825362	22.72299650255051
40-44	22.57550732441346	27.332463190063255	27.379768075719234	22.712261409804047
45-49	22.734863057514474	27.294632443012674	27.236681622983838	22.73382287648901
50-54	22.70514359964409	27.28201865596511	27.311852746030148	22.700984998360653
55-59	22.665544544850217	27.306195434942676	27.280923281660748	22.74733673854636
60-64	22.677360152172117	27.316764947852434	27.32333507086021	22.68253982911524
65-69	22.662424001773825	27.309405136392677	27.275484620870465	22.75268624096303
70-74	22.590583580827424	27.34512367911604	27.35146029209769	22.712832447958842
75-79	22.646698162923553	27.336989038933236	27.26243777886537	22.753875019277846
80-84	22.601278340023924	27.345268030768555	27.310765862999453	22.742687766208064
85-89	22.591538849116116	27.35530683907349	27.26996317016183	22.78319114164856
90-94	21.976137787637885	27.205382307312636	28.51763541540344	22.30084448964604
95-99	22.433806188057158	27.339027752720753	27.601959379493003	22.625206679729086
100-104	22.46813013762703	27.475888862943993	27.464343406623442	22.59163759280554
105-109	22.501169907764993	27.508115209772495	27.45215735593942	22.538557526523093
110-114	22.489651117257477	27.32321951159943	27.50033400709756	22.686795364045533
115-119	22.483303386146762	27.401737759871136	27.392851813612427	22.722107040369668
120-124	22.529922279036995	27.418591358167475	27.355470531688127	22.6960158311074
125-129	22.458767258229063	27.42140050947735	27.37819121343869	22.741641018854892
130-134	22.562331840287207	27.383160372036823	27.340839622526097	22.71366816514987
135-139	22.687755988359687	27.37385149154617	27.265529443676982	22.672863076417162
140-144	22.63705249328932	27.39759694727617	27.340662017955786	22.62468854147872
145-149	22.568612873826712	27.461394813533303	27.327487596956974	22.64250471568301
150-154	22.563719680704818	27.48763418054209	27.4024472148922	22.546198923860885
155-159	22.522394724735854	27.52527841467271	27.371016228510047	22.58131063208139
160-164	22.431223333414973	27.59890182315227	27.472200660102402	22.497674183330354
165-169	22.453157565562957	27.635179303330908	27.422207996324726	22.48945513478141
170-174	22.374145296850646	27.694567356889827	27.50056185568544	22.430725490574087
175-179	22.320780477953203	27.748842979037843	27.486996934701846	22.443379608307108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.0
10	3.5
11	12.5
12	26.5
13	63.5
14	136.0
15	254.5
16	466.0
17	877.5
18	1584.5
19	2781.5
20	4707.0
21	7677.0
22	12178.5
23	18490.0
24	26777.5
25	36801.5
26	48728.0
27	62627.5
28	78369.5
29	95818.0
30	114072.5
31	133497.5
32	154123.5
33	175002.0
34	196758.0
35	220275.5
36	243926.0
37	267831.5
38	291942.0
39	314411.5
40	336179.0
41	356920.0
42	374169.5
43	387408.0
44	395993.0
45	398998.5
46	397619.5
47	390894.5
48	377886.5
49	360045.0
50	338075.5
51	313322.5
52	286707.5
53	260449.5
54	235418.0
55	211634.0
56	190359.0
57	171147.5
58	154068.0
59	139367.0
60	126089.0
61	113965.0
62	102388.5
63	90957.0
64	79951.0
65	68968.0
66	58423.5
67	48456.0
68	38956.0
69	30329.0
70	23175.5
71	17199.5
72	12395.5
73	8609.5
74	5761.5
75	3807.5
76	2405.0
77	1448.0
78	853.0
79	511.5
80	276.5
81	125.5
82	63.0
83	39.5
84	15.5
85	5.0
86	3.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	901.0
92-93	1812.0
94-95	2791.0
96-97	3460.0
98-99	4250.0
100-101	5167.0
102-103	6204.0
104-105	6438.0
106-107	6407.0
108-109	7928.0
110-111	8230.0
112-113	7655.0
114-115	8332.0
116-117	8819.0
118-119	9205.0
120-121	9479.0
122-123	10746.0
124-125	10123.0
126-127	10827.0
128-129	11199.0
130-131	11468.0
132-133	12733.0
134-135	88421.0
136-137	89511.0
138-139	96972.0
140-141	95162.0
142-143	93074.0
144-145	79302.0
146-147	70017.0
148-149	78670.0
150-151	76160.0
152-153	76033.0
154-155	65762.0
156-157	77669.0
158-159	68544.0
160-161	71956.0
162-163	89531.0
164-165	74099.0
166-167	78035.0
168-169	74852.0
170-171	86369.0
172-173	81910.0
174-175	83926.0
176-177	91422.0
178-179	7469866.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.38314946249564
#Duplication Level	Percentage of total
1	91.21829449578094
2	7.605212682069473
3	0.9091288469392639
4	0.1746695952540886
5	0.04798312523911459
6	0.01744766318013944
7	0.008254140881273582
8	0.003111663003669878
9	0.00625615978920096
>10	0.009641627862844183
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.1842276289699755E-5	0.0	0.0	1.0614092096566586E-5	1.0614092096566586E-5	0.0
2	5.307046048283293E-5	0.0	0.0	1.0614092096566586E-5	2.1228184193133172E-5	0.0
3	5.307046048283293E-5	0.0	0.0	2.1228184193133172E-5	2.1228184193133172E-5	0.0
4	6.368455257939951E-5	1.0614092096566586E-5	0.0	3.1842276289699755E-5	2.1228184193133172E-5	0.0
5	7.429864467596609E-5	1.0614092096566586E-5	1.0614092096566586E-5	3.1842276289699755E-5	2.1228184193133172E-5	0.0
6	7.429864467596609E-5	1.0614092096566586E-5	1.0614092096566586E-5	3.1842276289699755E-5	2.1228184193133172E-5	0.0
7	7.429864467596609E-5	2.1228184193133172E-5	1.0614092096566586E-5	5.307046048283293E-5	2.1228184193133172E-5	0.0
8	7.429864467596609E-5	2.1228184193133172E-5	1.0614092096566586E-5	7.429864467596609E-5	2.1228184193133172E-5	0.0
9	7.429864467596609E-5	2.1228184193133172E-5	1.0614092096566586E-5	7.429864467596609E-5	2.1228184193133172E-5	0.0
10-14	9.128119203047264E-5	5.0947642063519605E-5	1.0614092096566586E-5	8.491273677253269E-5	3.1842276289699755E-5	0.0
15-19	1.1887783148154575E-4	8.703555519184601E-5	2.9719457870386436E-5	1.1038655780429248E-4	3.1842276289699755E-5	0.0
20-24	1.3373756041673899E-4	1.1887783148154575E-4	4.6702005224892975E-5	1.4222883409399225E-4	4.2456368386266345E-5	0.0
25-29	1.4647447093261886E-4	1.4435165251330554E-4	6.368455257939952E-5	1.9317647615751187E-4	5.943891574077287E-5	0.0
30-34	2.1652747876995835E-4	1.5496574460987215E-4	1.1463219464291914E-4	2.2501875244721161E-4	7.005300783733946E-5	0.0
35-39	4.776341443454963E-4	1.8256238406094528E-4	1.3161474199742564E-4	2.6110666557553803E-4	9.340401044978595E-5	4.245636838626635E-6
40-44	7.068985336313346E-4	1.9317647615751187E-4	1.8256238406094528E-4	3.1629994447768424E-4	1.4859728935193219E-4	2.759663945107312E-5
45-49	8.385132756287602E-4	2.228959340278983E-4	2.0803620509270504E-4	3.566334944446373E-4	2.0591338667339177E-4	3.1842276289699755E-5
50-54	9.786192913034393E-4	2.5473821031759804E-4	2.228959340278983E-4	4.054583180888436E-4	2.3563284454377822E-4	5.0947642063519605E-5
55-59	0.001044426662302152	2.865804866072978E-4	2.3563284454377817E-4	4.3093213912060336E-4	3.354053102515041E-4	5.307046048283293E-5
60-64	0.0010826373938497915	3.056858523811176E-4	2.4412411822103148E-4	4.840025996034363E-4	3.821073154763971E-4	5.307046048283293E-5
65-69	0.0011717957674609511	3.247912181549375E-4	2.5261539189828475E-4	5.264589679897027E-4	3.9908986283090366E-4	7.429864467596609E-5
70-74	0.002029414408863531	3.6300194970257727E-4	2.738435760914179E-4	5.774066100532222E-4	4.203180470240367E-4	8.491273677253269E-5
75-79	0.017411356675207827	4.1182677334678354E-4	2.95071760284551E-4	6.15617341600862E-4	4.4791468647510993E-4	8.491273677253269E-5
80-84	0.024053655509239193	4.7126568908755634E-4	3.0356303396180436E-4	6.538280731485016E-4	5.243361495703893E-4	9.552682886909927E-5
85-89	0.02410884878814134	5.137220574738228E-4	3.2691403657425077E-4	7.344951730824077E-4	7.36617991501721E-4	9.552682886909927E-5
90-94	0.02412795415391516	5.476871521828358E-4	3.3752812867081735E-4	7.918112704038671E-4	7.875656335652405E-4	9.552682886909927E-5
95-99	0.024168287703882112	5.943891574077287E-4	3.7998449705708385E-4	8.130394545970004E-4	7.960569072424939E-4	9.552682886909927E-5
100-104	0.024191638706494562	6.347227073746818E-4	3.905985891536503E-4	8.385132756287602E-4	8.024253625004338E-4	9.552682886909927E-5
105-109	0.024231972256461513	6.686878020836949E-4	4.0758113650815684E-4	8.915837361115932E-4	8.257763651128803E-4	9.552682886909927E-5
110-114	0.024259568895912586	6.856703494382015E-4	4.2668650228197677E-4	9.531454702716794E-4	8.406360940480734E-4	9.552682886909927E-5
115-119	0.024319007811653363	7.026528967927079E-4	4.3517777595923E-4	0.0010104615675931388	8.618642782412067E-4	9.552682886909927E-5
120-124	0.024437885643134906	7.153898073085878E-4	4.733885075068697E-4	0.0010486722991407786	8.915837361115932E-4	9.552682886909927E-5
125-129	0.024701115127129754	7.217582625665278E-4	5.158448758931361E-4	0.0011081112148815515	9.616367439489327E-4	9.552682886909927E-5
130-134	0.024911274150641777	7.217582625665278E-4	5.476871521828358E-4	0.0011314622174939978	0.001004093112335199	9.552682886909927E-5
135-139	0.025106573445218605	7.281267178244677E-4	5.731609732145956E-4	0.001165427312203011	0.0010486722991407786	9.552682886909927E-5
140-144	0.025401645205503153	7.366179915017211E-4	5.943891574077287E-4	0.001212129317427904	0.0010890058491077317	9.552682886909927E-5
145-149	0.025991788726072253	7.557233572755409E-4	6.453367994712484E-4	0.001239725956878977	0.0011293393990746846	9.552682886909927E-5
150-154	0.026671090620252514	7.727059046300475E-4	6.750562573416347E-4	0.0012673225963300504	0.001190901133234771	1.0614092096566585E-4
155-159	0.026968285198956377	7.790743598879873E-4	6.89915986276828E-4	0.0012821823252652435	0.0012482172305562304	1.0614092096566585E-4
160-164	0.027116882488308312	8.066709993390604E-4	7.111441704699612E-4	0.0013161474199742565	0.0012821823252652435	1.0614092096566585E-4
165-168	0.02731005896446582	8.17285091435627E-4	7.111441704699612E-4	0.0013532967423122398	0.0013028798048535485	1.0614092096566585E-4
>>END_MODULE
