##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293889
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9660824
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.386885632115856	39.0	39.0	39.0	37.0	39.0
2	40.156313581533006	41.0	40.0	41.0	38.0	41.0
3	40.18492946357371	41.0	40.0	41.0	38.0	41.0
4	40.19744589074389	41.0	40.0	41.0	38.0	41.0
5	40.19277123773293	41.0	40.0	41.0	38.0	41.0
6	40.191817178327646	41.0	40.0	41.0	38.0	41.0
7	40.22053957302193	41.0	40.0	41.0	39.0	41.0
8	40.18588755990172	41.0	40.0	41.0	38.0	41.0
9	40.19208527140128	41.0	40.0	41.0	38.0	41.0
10-14	40.13065750913172	41.0	40.0	41.0	38.0	41.0
15-19	40.03727539182993	41.0	40.0	41.0	38.0	41.0
20-24	39.91391694952729	41.0	40.0	41.0	38.0	41.0
25-29	39.72196421340457	40.8	39.8	41.0	37.2	41.0
30-34	39.453773197814186	40.0	39.0	41.0	36.2	41.0
35-39	39.06514487791104	40.0	38.0	41.0	35.0	41.0
40-44	38.666353015022324	40.0	37.0	41.0	35.0	41.0
45-49	38.23908730766651	39.2	35.8	41.0	35.0	41.0
50-54	37.72020034729957	38.0	35.0	40.6	35.0	41.0
55-59	36.98754748042196	36.6	35.0	39.4	34.6	41.0
60-64	36.131327948837495	35.4	34.8	38.0	33.6	40.2
65-69	35.66090329354928	35.0	35.0	36.6	33.8	39.0
70-74	35.375730496694686	35.0	35.0	36.0	34.0	37.6
75-79	35.14615440670486	35.0	35.0	35.2	34.0	36.8
80-84	35.04391347984396	35.0	35.0	35.0	34.0	36.0
85-89	34.9198445805451	35.0	34.8	35.0	33.6	36.0
90-94	39.35617479093294	40.2	39.6	40.2	37.6	40.2
95-99	39.98571649532217	41.0	40.0	41.0	38.0	41.0
100-104	39.89043446582732	41.0	40.0	41.0	38.0	41.0
105-109	39.77869822638552	41.0	40.0	41.0	37.0	41.0
110-114	39.617682275706635	41.0	39.4	41.0	36.6	41.0
115-119	39.408040867839254	40.2	39.0	41.0	35.6	41.0
120-124	39.12540771819606	40.0	38.0	41.0	35.0	41.0
125-129	38.46702827542972	39.6	37.2	40.6	34.6	40.8
130-134	38.17475215795667	39.4	36.2	40.6	34.6	40.8
135-139	38.189002449622684	39.0	35.6	41.0	35.0	41.0
140-144	37.63749409660845	38.0	35.0	40.8	35.0	41.0
145-149	36.90607687299375	36.4	35.0	39.4	34.0	41.0
150-154	36.19251478027422	35.4	35.0	37.8	34.0	40.2
155-159	35.58474534119652	35.0	35.0	36.4	34.0	38.6
160-164	35.143138561574574	35.0	35.0	35.8	34.0	37.0
165-169	34.873826561734646	35.0	35.0	35.0	34.0	36.0
170-174	34.72716740420862	35.0	35.0	35.0	33.8	36.0
175-179	34.56096608789154	35.0	34.8	35.0	32.8	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	5.0
29	57.0
30	591.0
31	3995.0
32	19517.0
33	67325.0
34	184248.0
35	452946.0
36	1234541.0
37	2931042.0
38	3690322.0
39	1064174.0
40	12061.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.062020796569733	27.568994114787728	27.669834374376347	22.699150714266196
2	22.716809663440717	27.60434306638854	28.219694303508685	21.459152966662057
3	21.060967470269617	26.991434685074484	29.23126433107569	22.71633351358021
4	21.63629106585525	26.302652858596744	29.645069613109605	22.4159864624384
5	22.362057315193816	26.797476074504615	28.091144192255236	22.74932241804633
6	22.52410353402567	27.054741914354302	27.716341794447345	22.70481275717268
7	22.448478514876165	27.48073042216689	27.565267724575047	22.505523338381902
8	22.46877699045133	27.496857410920644	27.498368669173562	22.535996929454466
9	22.724076124355438	27.500594152217246	27.308995588782075	22.466334134645244
10-14	22.562657181209385	27.42014759817589	27.44591351627977	22.57128170433495
15-19	22.570532285858846	27.459343012562904	27.432844237717198	22.53728046386105
20-24	22.560793986103047	27.277319201757532	27.5114565796872	22.65043023245222
25-29	22.549532006793623	27.37729825116367	27.379689351550134	22.69348039049257
30-34	22.585065207688288	27.345454176579555	27.403863272946488	22.66561734278567
35-39	22.508860527839033	27.40308073100183	27.39245637846213	22.695602362697013
40-44	22.55414652000699	27.35274548009569	27.378478274731016	22.7146297251663
45-49	22.72063335384228	27.330875709980845	27.237486160600792	22.71100477557608
50-54	22.685052537961564	27.30181607697232	27.32311032682098	22.690021058245136
55-59	22.639484996310873	27.32597757706796	27.309798832894582	22.72473859372658
60-64	22.6412819444801	27.340249651582514	27.336840004537915	22.68162839939947
65-69	22.639944584437107	27.32711619629961	27.301393752748215	22.731545466515072
70-74	22.566826597814014	27.366603511253285	27.361266492382015	22.705303398550683
75-79	22.613131136640106	27.355153142216444	27.297599045381638	22.734116675761822
80-84	22.563000837195666	27.3680195395341	27.335825598313352	22.733154024956878
85-89	22.55299133904106	27.380995658341362	27.289964085879216	22.776048916738365
90-94	21.937560115434888	27.222279320555415	28.554065505305893	22.286095058703804
95-99	22.39680194761339	27.37740569914584	27.614107717048462	22.611684636192308
100-104	22.43537205650111	27.51368499391974	27.49056021731234	22.560382732266813
105-109	22.45347898305403	27.535508724302954	27.49081775847878	22.520194534164236
110-114	22.46650607929008	27.357163254871576	27.51436726001763	22.661963405820714
115-119	22.442923687460528	27.441005897168658	27.405404686281244	22.71066572908957
120-124	22.490629339055246	27.432970366000042	27.4003905770287	22.67600971791601
125-129	22.425318431659658	27.460195610198074	27.3949739038759	22.71951205426637
130-134	22.527781876725673	27.40243997557833	27.370042340046346	22.69973580764965
135-139	22.645441269782562	27.415202802478824	27.284606270107897	22.65474965763072
140-144	22.604613454913647	27.423002626125182	27.365261198333386	22.607122720627785
145-149	22.539002496568024	27.483474153431214	27.358484545699397	22.61903880430137
150-154	22.537378319294962	27.536339919186332	27.39936232793329	22.52691943358542
155-159	22.47923224760973	27.56520627756786	27.403323906221207	22.552237568601203
160-164	22.384511641161765	27.632205436504986	27.496365412412498	22.486917509920755
165-169	22.402892940163703	27.678404281500296	27.459798573533938	22.45890420480206
170-174	22.32529681282791	27.743162754580293	27.523625053223444	22.407915379368358
175-179	22.277709866700523	27.810021529949445	27.50178505449035	22.410483548859673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	6.0
11	14.0
12	27.0
13	61.5
14	142.0
15	266.5
16	500.0
17	896.5
18	1603.5
19	2883.5
20	4904.5
21	8147.0
22	12952.5
23	19373.0
24	27495.5
25	37832.5
26	50594.0
27	65267.5
28	81253.0
29	99047.5
30	117870.5
31	137547.5
32	158702.5
33	181054.5
34	204058.5
35	226989.0
36	251256.0
37	276206.0
38	300428.5
39	323229.0
40	345054.0
41	365750.0
42	383218.5
43	396775.5
44	405497.5
45	409516.0
46	408106.0
47	400147.0
48	386481.0
49	368502.0
50	346528.0
51	321178.0
52	294044.5
53	267045.5
54	240520.0
55	215533.0
56	193576.5
57	174317.5
58	157189.0
59	142020.0
60	128837.5
61	116441.5
62	104874.0
63	93046.5
64	81490.0
65	70341.0
66	59081.5
67	48798.5
68	39353.0
69	30710.5
70	23268.5
71	17263.0
72	12393.5
73	8625.0
74	5845.5
75	3761.5
76	2350.5
77	1466.5
78	840.5
79	469.0
80	266.0
81	130.0
82	60.5
83	24.5
84	12.0
85	5.0
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	873.0
92-93	1705.0
94-95	2645.0
96-97	3385.0
98-99	4079.0
100-101	4666.0
102-103	5199.0
104-105	5770.0
106-107	6397.0
108-109	6943.0
110-111	7316.0
112-113	7826.0
114-115	8336.0
116-117	8805.0
118-119	9219.0
120-121	9676.0
122-123	10043.0
124-125	10483.0
126-127	13095.0
128-129	11737.0
130-131	11861.0
132-133	12688.0
134-135	71397.0
136-137	79794.0
138-139	84301.0
140-141	80179.0
142-143	79916.0
144-145	72173.0
146-147	71357.0
148-149	71472.0
150-151	73901.0
152-153	73521.0
154-155	68249.0
156-157	80222.0
158-159	76471.0
160-161	72106.0
162-163	70549.0
164-165	75017.0
166-167	78367.0
168-169	77892.0
170-171	84320.0
172-173	80639.0
174-175	88626.0
176-177	84886.0
178-179	7802722.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.15844783064438
#Duplication Level	Percentage of total
1	90.81904601194682
2	7.870293180865271
3	1.0239932370045486
4	0.18474742146094178
5	0.04795664520837666
6	0.019486980540004507
7	0.010309249242743687
8	0.0072536608161467755
9	0.006249405344066522
>10	0.010664207571069157
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.070216784820839E-5	0.0	0.0	0.0	0.0	0.0
2	4.140433569641678E-5	0.0	1.0351083924104196E-5	1.0351083924104196E-5	0.0	0.0
3	4.140433569641678E-5	1.0351083924104196E-5	1.0351083924104196E-5	3.105325177231259E-5	0.0	0.0
4	4.140433569641678E-5	1.0351083924104196E-5	1.0351083924104196E-5	4.140433569641678E-5	0.0	0.0
5	4.140433569641678E-5	2.070216784820839E-5	1.0351083924104196E-5	5.175541962052098E-5	0.0	0.0
6	4.140433569641678E-5	2.070216784820839E-5	1.0351083924104196E-5	7.245758746872937E-5	0.0	0.0
7	4.140433569641678E-5	2.070216784820839E-5	1.0351083924104196E-5	8.280867139283357E-5	0.0	0.0
8	5.175541962052098E-5	2.070216784820839E-5	1.0351083924104196E-5	8.280867139283357E-5	0.0	0.0
9	5.175541962052098E-5	2.070216784820839E-5	1.0351083924104196E-5	9.315975531693777E-5	0.0	0.0
10-14	5.3825636405341823E-5	2.6912818202670912E-5	3.519368534195426E-5	1.2628322387407117E-4	1.0351083924104196E-5	0.0
15-19	7.038737068390854E-5	6.624693711426686E-5	6.0036286759804344E-5	2.153025456213673E-4	1.0351083924104196E-5	0.0
20-24	7.866823782319188E-5	8.487888817765441E-5	7.452780425355022E-5	2.8154948273563415E-4	2.070216784820839E-5	0.0
25-29	8.694910496247525E-5	1.1800235673478784E-4	1.4698539172227956E-4	3.1260273450794675E-4	4.347455248123762E-5	0.0
30-34	1.5526625886156295E-4	1.2421300708925035E-4	1.6561734278566713E-4	3.747092380525719E-4	6.83171538990877E-5	0.0
35-39	2.8776013309009664E-4	1.2835344065889202E-4	2.0081102812762142E-4	4.1611357374898877E-4	1.1386192316514617E-4	2.484260141785007E-5
40-44	3.9748162268560115E-4	1.5940669243120462E-4	2.1737276240618811E-4	4.740796437239722E-4	1.6975777635530882E-4	4.5544769266058456E-5
45-49	4.823605108632556E-4	1.8838972741869637E-4	2.1737276240618811E-4	5.27905280129314E-4	2.215131959758298E-4	6.210650354462518E-5
50-54	5.775904829650142E-4	1.9667059455797973E-4	2.5256644774814246E-4	5.610287486864475E-4	2.6498774845706743E-4	7.038737068390852E-5
55-59	6.83171538990877E-4	2.1530254562136727E-4	2.794792659508133E-4	6.18994818661431E-4	3.001814337990217E-4	9.730018888657945E-5
60-64	7.59769560029248E-4	2.4428558060885907E-4	3.1881338486240923E-4	6.521182872185644E-4	3.892007555463178E-4	1.0351083924104195E-4
65-69	8.322271474979773E-4	2.484260141785007E-4	3.581475037740052E-4	6.811013222060561E-4	4.4095617516683877E-4	1.055810560258628E-4
70-74	0.001714139497831655	2.670579652418883E-4	4.036922730400637E-4	6.997332732694437E-4	4.699392101543305E-4	1.1386192316514617E-4
75-79	0.016785317691327368	2.8154948273563415E-4	4.3474552481237623E-4	7.659802103837105E-4	5.27905280129314E-4	1.1386192316514617E-4
80-84	0.023300289913158544	3.0018143379902167E-4	4.595881262302263E-4	8.053143292953065E-4	6.624693711426685E-4	1.1386192316514617E-4
85-89	0.02336653685027281	3.3537511914097595E-4	4.8029029407843475E-4	8.239462803586939E-4	9.357379867390193E-4	1.1386192316514617E-4
90-94	0.02341622205310851	3.5607728698918437E-4	4.88571161217718E-4	8.591399657006483E-4	9.688614552961527E-4	1.1386192316514617E-4
95-99	0.02347832855665314	3.643581541284677E-4	4.968520283570014E-4	8.860527839033192E-4	9.730018888657944E-4	1.1386192316514617E-4
100-104	0.023525943542704018	3.912709723311386E-4	5.258350633444932E-4	9.481592874479444E-4	9.833529727898986E-4	1.1386192316514617E-4
105-109	0.023592190479818285	4.368157415971971E-4	5.651691822560891E-4	0.0010516701266889863	9.833529727898986E-4	1.1386192316514617E-4
110-114	0.023666718284071835	4.595881262302263E-4	5.9208200045876E-4	0.0011137766302336115	9.833529727898986E-4	1.1386192316514617E-4
115-119	0.02368535023513522	4.823605108632556E-4	6.003628675980434E-4	0.0011613916162844908	9.937040567140028E-4	1.1386192316514617E-4
120-124	0.023803352591870008	5.27905280129314E-4	6.086437347373268E-4	0.0012069363855505494	0.001028897742055957	1.1386192316514617E-4
125-129	0.023937916682883364	5.548180983319849E-4	6.18994818661431E-4	0.0012421300708925036	0.001082723378461299	1.1386192316514617E-4
130-134	0.024134587277441347	5.817309165346558E-4	6.272756858007143E-4	0.0012897450569433829	0.0011427596652211032	1.2007257351960869E-4
135-139	0.024318836571290397	6.045033011676851E-4	6.31416119370356E-4	0.0013125174415764122	0.001198655518411266	1.4491517493745874E-4
140-144	0.024565192368684083	6.252054690158935E-4	6.583289375730268E-4	0.0013270089590701579	0.0012421300708925036	1.5112582529192128E-4
145-149	0.02529183846015619	6.500480704337435E-4	6.748906718515936E-4	0.001403606980108529	0.0013145876583612328	1.5526625886156295E-4
150-154	0.026072310188033652	6.666098047123102E-4	6.83171538990877E-4	0.0014615730500835125	0.0013497813437031872	1.6561734278566713E-4
155-159	0.026256559481882706	6.769608886364144E-4	6.83171538990877E-4	0.0014781347843620793	0.0013829048122603208	1.6561734278566713E-4
160-164	0.026368351188263035	6.873119725605187E-4	7.142247907631896E-4	0.00150504760256475	0.0014284495815263792	1.7596842670977134E-4
165-168	0.026535003639441106	7.012859358580593E-4	7.401025005734501E-4	0.0015371359627294732	0.001472441688203822	1.7596842670977134E-4
>>END_MODULE
