##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293890
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9078459
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.31825180903499	39.0	39.0	39.0	37.0	39.0
2	40.02728833164307	41.0	40.0	41.0	38.0	41.0
3	40.101983387268696	41.0	40.0	41.0	38.0	41.0
4	40.133780523765104	41.0	40.0	41.0	38.0	41.0
5	40.144028077892955	41.0	40.0	41.0	38.0	41.0
6	40.13950374176939	41.0	40.0	41.0	38.0	41.0
7	40.16308593782271	41.0	40.0	41.0	38.0	41.0
8	40.14536200471908	41.0	40.0	41.0	38.0	41.0
9	40.12413086846567	41.0	40.0	41.0	38.0	41.0
10-14	40.06637196907537	41.0	40.0	41.0	38.0	41.0
15-19	39.936406498063164	41.0	40.0	41.0	38.0	41.0
20-24	39.74932714902386	40.8	40.0	41.0	37.6	41.0
25-29	39.50983104070856	40.0	39.0	41.0	36.6	41.0
30-34	39.33494362864887	40.0	38.8	41.0	35.6	41.0
35-39	39.01125276877937	40.0	37.8	41.0	35.0	41.0
40-44	38.505354884567964	39.8	36.6	41.0	35.0	41.0
45-49	38.01509544736612	39.0	35.4	41.0	35.0	41.0
50-54	37.31124907872581	37.2	35.0	40.0	34.0	41.0
55-59	36.58288290997403	36.2	35.0	39.0	34.0	41.0
60-64	35.65217035181852	34.8	34.6	37.0	33.4	39.6
65-69	35.38253300477537	35.0	34.8	36.0	33.6	38.8
70-74	35.2584323396735	35.0	35.0	36.0	34.0	37.2
75-79	35.08748909919624	35.0	35.0	35.0	33.4	36.8
80-84	35.00940948238021	35.0	35.0	35.0	33.0	36.0
85-89	34.87626221586724	35.0	34.8	35.0	32.6	36.2
90-94	39.58452291090199	40.6	39.8	40.6	37.8	40.6
95-99	39.897218935367206	41.0	40.0	41.0	38.0	41.0
100-104	39.78199607581487	41.0	40.0	41.0	37.0	41.0
105-109	39.62451924933909	41.0	39.4	41.0	36.6	41.0
110-114	39.42853055361989	40.2	39.0	41.0	35.6	41.0
115-119	39.148500345891	40.0	38.2	41.0	35.0	41.0
120-124	38.871709331293005	40.0	38.0	41.0	35.0	41.0
125-129	38.18385951122076	39.4	36.4	40.4	34.4	40.6
130-134	38.11266110453452	39.0	35.6	40.8	34.4	41.0
135-139	37.58530001175081	38.2	35.0	40.6	34.0	41.0
140-144	36.85134297231232	36.6	35.0	39.4	34.0	41.0
145-149	36.23645919274486	35.6	35.0	38.4	33.6	40.6
150-154	35.73886740772498	35.0	35.0	36.8	34.0	39.0
155-159	35.22761276139578	35.0	35.0	36.0	34.0	37.2
160-164	34.88943172511533	35.0	35.0	35.0	33.4	36.2
165-169	34.692610410720974	35.0	35.0	35.0	33.0	36.0
170-174	34.58664683945683	35.0	35.0	35.0	33.0	35.8
175-178	34.342955081751555	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	141.0
31	2218.0
32	14654.0
33	61732.0
34	195176.0
35	530059.0
36	1441588.0
37	3084839.0
38	3153642.0
39	591682.0
40	2722.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.75664394144425	27.41010341072202	27.931072883624854	22.90217976420888
2	22.517984605096526	27.71594826831294	28.294240244957873	21.471826881632662
3	21.06177931739296	26.90791465820356	29.118521105839655	22.91178491856382
4	21.632261598581874	26.298372884649257	29.408603376410024	22.66076214035884
5	22.18974607915286	27.00934156336444	28.0913864346361	22.7095259228466
6	22.46981563721332	26.987157181631815	27.679047732660354	22.863979448494508
7	22.438698021327188	27.37767499968882	27.501143090473835	22.682483888510156
8	22.335244340476727	27.57033985613638	27.541821800373828	22.552594003013066
9	22.719769952147164	27.446904810607176	27.163629862733313	22.66969537451235
10-14	22.471492133191326	27.512960073950875	27.38394919225829	22.631598600599506
15-19	22.539023417961133	27.400456399043055	27.398310660432568	22.662209522563245
20-24	22.45418963725011	27.344052553412425	27.477630289457718	22.72412751987975
25-29	22.461371472845777	27.354054250837063	27.386720587712077	22.797853688605084
30-34	22.51759687409504	27.38957349479686	27.344726676630916	22.748102954477186
35-39	22.432948146816546	27.385193896893732	27.40867144963699	22.773186506652728
40-44	22.507432153408413	27.410105613739074	27.331642958347885	22.75081927450463
45-49	22.716824518346122	27.266158276421145	27.22433840368723	22.792678801545506
50-54	22.63712376737065	27.3533647064992	27.279900696803278	22.72961082932687
55-59	22.604909048991683	27.323137109502838	27.287327067291926	22.784626774213553
60-64	22.645898384296277	27.331484341120003	27.25294017409783	22.769677100485886
65-69	22.575586892004466	27.31279174141779	27.327518910423017	22.784102456154727
70-74	22.533621620145006	27.366620260112427	27.324165918466996	22.77559220127557
75-79	22.57913154644417	27.316389268266782	27.2845336416676	22.819945543621444
80-84	22.552798883599078	27.341893596699617	27.291982042326783	22.81332547737452
85-89	22.509886314406444	27.349855300332358	27.29142908504626	22.848829300214938
90-94	21.869899435300468	27.130202049949165	28.436442361644165	22.56345615310621
95-99	22.43232071078925	27.469157398734527	27.44421405932395	22.65430783115227
100-104	22.48743242071498	27.48153675114641	27.396875488429302	22.63415533970931
105-109	22.402762363604268	27.495538604006565	27.481382086295536	22.62031694609363
110-114	22.48284918624507	27.350506032283178	27.443130278785098	22.72351450268666
115-119	22.47893940284599	27.400235531024737	27.353382561274724	22.76744250485455
120-124	22.418388464518273	27.465884469037892	27.395086418374053	22.720640648069782
125-129	22.460390311904316	27.41446213153438	27.398415095699608	22.7267324608617
130-134	22.525310610338327	27.43725125468146	27.325630963666015	22.711807171314202
135-139	22.577540312746365	27.418584892927512	27.30374109265706	22.700133701669067
140-144	22.6234431147223	27.466016877844957	27.280127366785116	22.630412640647624
145-149	22.511705190056414	27.527338917770734	27.34554656876842	22.615409323404435
150-154	22.43114459735287	27.62574652628793	27.435521328534186	22.50758754782502
155-159	22.442089934940242	27.58109372411536	27.46512281744527	22.511693523499137
160-164	22.375918952936537	27.7111641581474	27.473672670387106	22.43924421852896
165-169	22.284098634549167	27.749597822602023	27.536014291831545	22.430289251017264
170-174	22.317810941557624	27.815344953232433	27.531354072004323	22.33549003320562
175-178	22.19068345641594	27.895831371272834	27.599035151485367	22.31445002082586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	3.0
12	4.5
13	21.0
14	54.0
15	107.0
16	178.0
17	324.0
18	611.5
19	1172.5
20	2172.0
21	3790.0
22	6462.5
23	10479.0
24	16232.5
25	24445.5
26	34978.5
27	48024.0
28	63655.5
29	81727.5
30	102098.0
31	124317.0
32	147772.0
33	173034.5
34	199627.5
35	226187.0
36	252386.5
37	277599.5
38	302345.0
39	325612.0
40	345058.5
41	361848.5
42	375849.5
43	384433.5
44	388705.5
45	388715.5
46	383661.0
47	374069.0
48	360308.0
49	342178.0
50	321101.5
51	297911.0
52	273197.0
53	248509.0
54	223754.5
55	200204.5
56	178649.0
57	159413.5
58	142446.5
59	127461.5
60	114434.5
61	102694.5
62	91689.0
63	81467.5
64	72217.5
65	62958.5
66	53897.5
67	45571.0
68	37433.0
69	30095.5
70	23640.5
71	18012.0
72	13405.0
73	9637.0
74	6706.5
75	4623.0
76	3078.5
77	1903.0
78	1167.5
79	708.5
80	440.0
81	235.5
82	128.0
83	74.5
84	32.0
85	16.5
86	6.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	971.0
92-93	2204.0
94-95	3471.0
96-97	4589.0
98-99	6011.0
100-101	8118.0
102-103	8077.0
104-105	9694.0
106-107	9594.0
108-109	10722.0
110-111	11486.0
112-113	12897.0
114-115	12215.0
116-117	12902.0
118-119	13335.0
120-121	13979.0
122-123	14245.0
124-125	14816.0
126-127	15453.0
128-129	16268.0
130-131	16947.0
132-133	18179.0
134-135	92961.0
136-137	98394.0
138-139	94217.0
140-141	105660.0
142-143	99640.0
144-145	101653.0
146-147	89901.0
148-149	83819.0
150-151	83550.0
152-153	84506.0
154-155	76707.0
156-157	75684.0
158-159	84436.0
160-161	81687.0
162-163	82997.0
164-165	85101.0
166-167	90528.0
168-169	90651.0
170-171	95175.0
172-173	98909.0
174-175	105035.0
176-177	239853.0
178-179	6701222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.37331573214249
#Duplication Level	Percentage of total
1	87.6303814090655
2	10.191257217641624
3	1.5456406284176094
4	0.3423269655816418
5	0.12665506262502435
6	0.05648703270072368
7	0.032013157663322224
8	0.020766968666471795
9	0.012528416863199133
>10	0.041943140774837656
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2030170538854666E-5	0.0	0.0	2.2030170538854666E-5	0.0	0.0
2	3.3045255808282E-5	0.0	0.0	2.2030170538854666E-5	0.0	0.0
3	4.406034107770933E-5	2.2030170538854666E-5	0.0	2.2030170538854666E-5	0.0	0.0
4	5.507542634713667E-5	2.2030170538854666E-5	0.0	5.507542634713667E-5	0.0	0.0
5	6.6090511616564E-5	2.2030170538854666E-5	0.0	5.507542634713667E-5	1.1015085269427333E-5	0.0
6	6.6090511616564E-5	2.2030170538854666E-5	1.1015085269427333E-5	6.6090511616564E-5	1.1015085269427333E-5	0.0
7	6.6090511616564E-5	2.2030170538854666E-5	2.2030170538854666E-5	6.6090511616564E-5	1.1015085269427333E-5	0.0
8	6.6090511616564E-5	2.2030170538854666E-5	3.3045255808282E-5	7.710559688599133E-5	2.2030170538854666E-5	0.0
9	6.6090511616564E-5	2.2030170538854666E-5	3.3045255808282E-5	8.812068215541866E-5	2.2030170538854666E-5	0.0
10-14	6.829352867044946E-5	3.3045255808282E-5	6.388749456267854E-5	1.6302326198752454E-4	2.2030170538854666E-5	0.0
15-19	8.59176651015332E-5	6.6090511616564E-5	1.1015085269427334E-4	2.4012885887351583E-4	2.2030170538854666E-5	0.0
20-24	1.0794783564038786E-4	9.693275037096053E-5	1.32181023233128E-4	3.1503143870562176E-4	2.2030170538854666E-5	0.0
25-29	1.2116593796370067E-4	1.1015085269427334E-4	1.3658705734089893E-4	3.8112195032218574E-4	2.64362046466256E-5	0.0
30-34	1.8064739841860828E-4	1.1015085269427334E-4	1.5421119377198266E-4	4.8246073480091715E-4	4.62633581315948E-5	1.32181023233128E-5
35-39	4.163702231843532E-4	1.1015085269427334E-4	1.6302326198752452E-4	6.058296898185033E-4	5.9481460454907596E-5	5.0669392239365735E-5
40-44	6.124387409801597E-4	1.1675990385592973E-4	1.7624136431083733E-4	7.093714913511201E-4	7.930861393987681E-5	5.9481460454907596E-5
45-49	7.798680370754553E-4	1.387900743947844E-4	1.9827153484969201E-4	7.688529518060279E-4	1.2116593796370067E-4	7.710559688599133E-5
50-54	9.010339750391561E-4	1.6082024493363907E-4	2.2250472244243215E-4	8.437555316381338E-4	1.4099309144866985E-4	9.693275037096053E-5
55-59	0.0010133878447873145	1.8945946663415013E-4	2.445348929812868E-4	9.120490603085833E-4	1.7844438136472277E-4	1.2777498912535707E-4
60-64	0.0010971024928349625	2.1369265422689025E-4	2.621590294123705E-4	9.825456060329183E-4	2.114896371730048E-4	1.586172278797536E-4
65-69	0.0011477718850743282	2.5114394414294325E-4	2.8639221700511066E-4	0.0010596512029189095	2.335198077118595E-4	1.6522627904141E-4
70-74	0.0020510088771673696	2.5334696119682867E-4	3.084223875439654E-4	0.001114726629266046	2.5554997825071415E-4	1.9827153484969201E-4
75-79	0.020001191832226154	2.7537713173568333E-4	3.392646262983619E-4	0.0011675990385592975	2.7978316584345423E-4	2.1809868833466117E-4
80-84	0.027850541595220067	3.1062540459785075E-4	3.5688876272944555E-4	0.0012006442943675792	3.9654306969938403E-4	2.2691075655020308E-4
85-89	0.027938662277375494	3.679038479988729E-4	3.877310014838421E-4	0.0012469076524991743	6.675141673272965E-4	2.31316790657974E-4
90-94	0.027976113567291544	4.0315212086104046E-4	4.273853084537806E-4	0.0013482464369779057	6.719202014350673E-4	2.31316790657974E-4
95-99	0.028017970891315365	4.494154789926352E-4	4.472124619387498E-4	0.0014385701361872096	6.741232184889528E-4	2.31316790657974E-4
100-104	0.028051016147123648	4.670396154237189E-4	4.9567883712423E-4	0.0014892395284265753	6.829352867044946E-4	2.31316790657974E-4
105-109	0.028092873471147473	4.736486665853754E-4	5.066939223936573E-4	0.001524487801288743	6.829352867044946E-4	2.31316790657974E-4
110-114	0.028128121744009638	4.978818541781155E-4	5.33130127040283E-4	0.0016059994322825052	6.829352867044946E-4	2.31316790657974E-4
115-119	0.028183197170356777	5.066939223936573E-4	5.419421952558249E-4	0.0016875110632762675	6.873413208122655E-4	2.31316790657974E-4
120-124	0.028335205347074878	5.331301270402828E-4	5.595663316869085E-4	0.0017976619159705408	7.005594231355783E-4	2.31316790657974E-4
125-129	0.02854449196719399	5.749874510641067E-4	5.904085704413051E-4	0.0018483313082099065	7.247926107283185E-4	2.4233187592740134E-4
130-134	0.02879343289428305	5.837995192796487E-4	6.058296898185033E-4	0.0018923916492876159	7.534318324288296E-4	2.4233187592740134E-4
135-139	0.029154727691120264	5.94814604549076E-4	6.300628774112435E-4	0.001940858024473096	7.908831223448825E-4	2.533469611968286E-4
140-144	0.029562285846089074	6.080327068723887E-4	6.542960650039836E-4	0.0020069485360896602	7.996951905604244E-4	2.64362046466256E-4
145-149	0.030544831452121994	6.168447750879307E-4	6.719202014350673E-4	0.002048805860113484	8.129132928837372E-4	2.64362046466256E-4
150-154	0.03129606026749694	6.300628774112435E-4	6.719202014350673E-4	0.0021369265422689025	8.217253610992792E-4	2.64362046466256E-4
155-159	0.03147670766591555	6.56499082057869E-4	6.961533890278074E-4	0.0022074230879932377	8.547706169075612E-4	2.64362046466256E-4
160-164	0.03166837014960358	6.719202014350673E-4	7.159805425127766E-4	0.0022999498042564274	8.701917362847594E-4	2.64362046466256E-4
165-167	0.031881328464812515	6.719202014350673E-4	7.159805425127766E-4	0.0023425414672982128	8.701917362847594E-4	2.64362046466256E-4
>>END_MODULE
