##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293893
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9009476
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.33653055960191	39.0	39.0	39.0	37.0	39.0
2	40.03900237927267	41.0	40.0	41.0	38.0	41.0
3	40.10062505299975	41.0	40.0	41.0	38.0	41.0
4	40.13238083990678	41.0	40.0	41.0	38.0	41.0
5	40.14270485875094	41.0	40.0	41.0	38.0	41.0
6	40.13984309409337	41.0	40.0	41.0	38.0	41.0
7	40.16313323882543	41.0	40.0	41.0	38.0	41.0
8	40.13996829560343	41.0	40.0	41.0	38.0	41.0
9	40.123217598892545	41.0	40.0	41.0	38.0	41.0
10-14	40.06825442456365	41.0	40.0	41.0	38.0	41.0
15-19	39.938957471000535	41.0	40.0	41.0	38.0	41.0
20-24	39.755859008892415	40.8	40.0	41.0	37.6	41.0
25-29	39.51305456610351	40.0	39.0	41.0	36.6	41.0
30-34	39.33546985418464	40.0	38.8	41.0	35.6	41.0
35-39	39.00444785024123	40.0	37.8	41.0	35.0	41.0
40-44	38.49784970846251	39.8	36.6	41.0	35.0	41.0
45-49	38.00274275662647	38.8	35.4	41.0	35.0	41.0
50-54	37.29567715147918	37.2	35.0	39.8	34.0	41.0
55-59	36.56269567730688	36.0	35.0	39.0	34.0	40.8
60-64	35.659726758803735	34.8	34.6	37.0	33.4	39.4
65-69	35.37467235608376	35.0	34.8	36.0	33.6	38.8
70-74	35.242242523316555	35.0	35.0	35.8	34.0	37.0
75-79	35.069765611229776	35.0	35.0	35.0	33.2	36.6
80-84	34.98872322874272	35.0	35.0	35.0	33.0	36.0
85-89	34.85635841640512	35.0	34.8	35.0	32.6	36.0
90-94	39.59320649372397	40.6	39.8	40.6	37.8	40.6
95-99	39.899683674346555	41.0	40.0	41.0	38.0	41.0
100-104	39.78968113555743	41.0	40.0	41.0	37.2	41.0
105-109	39.624456544151386	41.0	39.4	41.0	36.6	41.0
110-114	39.4323333204544	40.2	39.0	41.0	35.8	41.0
115-119	39.158861694420274	40.0	38.2	41.0	35.0	41.0
120-124	38.881807630468195	40.0	38.0	41.0	35.0	41.0
125-129	38.215094571794864	39.4	36.4	40.4	34.4	40.6
130-134	38.12252494172205	39.0	35.6	40.8	34.4	41.0
135-139	37.582806087309216	38.2	35.0	40.6	34.0	41.0
140-144	36.844884346175114	36.6	35.0	39.4	34.0	41.0
145-149	36.230227441051866	35.6	35.0	38.4	33.6	40.2
150-154	35.726565303264195	35.0	35.0	36.8	34.0	39.0
155-159	35.22182877230664	35.0	35.0	36.0	34.0	37.2
160-164	34.8795821152457	35.0	35.0	35.0	33.4	36.2
165-169	34.68646165227639	35.0	35.0	35.0	33.0	36.0
170-174	34.57586804189269	35.0	35.0	35.0	33.0	35.8
175-178	34.34171762452708	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	9.0
30	170.0
31	2190.0
32	14687.0
33	61390.0
34	194530.0
35	525614.0
36	1437904.0
37	3080427.0
38	3120928.0
39	569971.0
40	1656.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.793842394385646	27.40658835208618	27.834737558543914	22.96483169498426
2	22.52760315916264	27.724575768890443	28.225614897026198	21.522206174920715
3	21.081891999046338	26.898390095051035	29.082235193256523	22.93748271264611
4	21.675955405175618	26.24412340961894	29.382618922565527	22.697302262639916
5	22.211802329014475	26.98253483332438	28.044339093638744	22.761323744022405
6	22.530511208421	26.945906731978642	27.659477643316883	22.864104416283478
7	22.489620928009575	27.348771449083166	27.48441751773355	22.677190105173707
8	22.379625629725858	27.55635288889165	27.50134414032514	22.56267734105735
9	22.73714919713422	27.389872618562944	27.166696487120895	22.706281697181947
10-14	22.502061163157546	27.481889068798232	27.348889102984458	22.667160665059765
15-19	22.55778915444139	27.372459841171676	27.381554709730064	22.68819629465687
20-24	22.478330593255368	27.31644992450171	27.451223578374588	22.753995903868326
25-29	22.4937898719082	27.314900444820545	27.366397335427724	22.824912347843537
30-34	22.554050868219193	27.343978717519196	27.31369726718846	22.78827314707315
35-39	22.471917345692468	27.356785233680625	27.377370226636934	22.793927193989973
40-44	22.539093283560554	27.38214297923653	27.293405299042917	22.78535843816
45-49	22.742059582599477	27.233130983422342	27.204532205868574	22.820277228109603
50-54	22.677034713228604	27.325575871449125	27.252970095042155	22.744419320280112
55-59	22.6267698587576	27.29993842039204	27.24934724283632	22.823944478014038
60-64	22.677649621354227	27.297694116727765	27.215398542601143	22.80925771931686
65-69	22.61140825504169	27.271202009972612	27.29414230083969	22.823247434146005
70-74	22.57517085344364	27.356110388661893	27.273335319390384	22.795383438504082
75-79	22.62371973686372	27.28243684760357	27.244674385058577	22.849169030474137
80-84	22.58451656899913	27.32570462477507	27.254739343331398	22.8350394628944
85-89	22.535246222976784	27.324883267350952	27.262049424406037	22.877821085266223
90-94	21.872275257414632	27.111935822315658	28.41863777657275	22.597151143696966
95-99	22.461299352633375	27.433620177299073	27.41963181748104	22.68544865258652
100-104	22.49974013069812	27.474270713252757	27.363218043365567	22.662771112683558
105-109	22.42775091108078	27.4672026872245	27.44911281890951	22.65593358278521
110-114	22.506249488971104	27.32743759830943	27.408586860724952	22.757726051994513
115-119	22.50483682061574	27.384904593578653	27.320766879435286	22.789491706370324
120-124	22.4517305567641	27.442199728318943	27.349244943473987	22.756824771442968
125-129	22.4958475913315	27.393518034642696	27.351256445791556	22.759377928234255
130-134	22.561344906166212	27.419858474707198	27.290247934019522	22.728548685107064
135-139	22.617366504245954	27.402299390922902	27.251443085261425	22.728891019569723
140-144	22.63753071616543	27.453942898772983	27.250728426102505	22.657797958959083
145-149	22.54144372643013	27.500425788568624	27.309884333981927	22.648246151019322
150-154	22.464938575999213	27.611423549029272	27.387625249847154	22.536012625124357
155-159	22.475870886441797	27.577977890315193	27.39899443377107	22.547156789471938
160-164	22.409874320896346	27.67221214112034	27.444548440634005	22.473365097349316
165-169	22.31762289562691	27.708162274453603	27.515987428672815	22.45822740124667
170-174	22.345322555371393	27.784052008546745	27.489605731400797	22.381019704681073
175-178	22.225850236650974	27.851527886059824	27.574656659142004	22.347965218147195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	3.0
12	6.0
13	16.5
14	38.0
15	75.0
16	166.0
17	327.5
18	601.5
19	1172.5
20	2123.5
21	3689.0
22	6215.5
23	10188.0
24	16007.5
25	23832.5
26	34166.5
27	46960.5
28	62332.0
29	80342.0
30	100279.5
31	122109.0
32	145976.5
33	170936.0
34	196669.0
35	223012.0
36	249063.5
37	274593.5
38	298755.0
39	321011.0
40	341281.0
41	358193.5
42	371114.5
43	380098.5
44	385295.0
45	385691.0
46	380931.0
47	371331.0
48	357740.5
49	340045.5
50	319390.5
51	296956.0
52	272139.5
53	247142.0
54	222924.0
55	199479.0
56	178252.0
57	159535.0
58	142633.5
59	127735.0
60	114679.5
61	103162.5
62	92354.5
63	82070.0
64	72689.5
65	63575.5
66	54247.5
67	45602.5
68	37814.5
69	30521.0
70	23953.5
71	18255.5
72	13541.5
73	9786.0
74	6824.0
75	4644.0
76	3093.0
77	1958.0
78	1216.5
79	745.0
80	422.0
81	221.0
82	118.5
83	56.0
84	30.5
85	20.5
86	5.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	991.0
92-93	2216.0
94-95	3476.0
96-97	4623.0
98-99	5776.0
100-101	6886.0
102-103	7825.0
104-105	8681.0
106-107	9500.0
108-109	10459.0
110-111	11145.0
112-113	11373.0
114-115	11851.0
116-117	12558.0
118-119	12725.0
120-121	13074.0
122-123	13614.0
124-125	14473.0
126-127	15053.0
128-129	15630.0
130-131	16553.0
132-133	18438.0
134-135	90011.0
136-137	94142.0
138-139	91360.0
140-141	94035.0
142-143	95967.0
144-145	99342.0
146-147	85637.0
148-149	81486.0
150-151	81800.0
152-153	79368.0
154-155	73335.0
156-157	73369.0
158-159	76101.0
160-161	78411.0
162-163	81548.0
164-165	82559.0
166-167	84859.0
168-169	89753.0
170-171	93325.0
172-173	97118.0
174-175	101774.0
176-177	209742.0
178-179	6747514.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.32619891958258
#Duplication Level	Percentage of total
1	87.51586663268101
2	10.313296760739977
3	1.5791165260135889
4	0.33830744069764845
5	0.1287198286754281
6	0.052389970282401034
7	0.024790641352650973
8	0.01350249094742154
9	0.010443835957107957
>10	0.023565872652768254
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.109942465022383E-5	2.219884930044766E-5	2.219884930044766E-5	1.109942465022383E-5	0.0	0.0
2	1.109942465022383E-5	2.219884930044766E-5	3.329827395067149E-5	1.109942465022383E-5	1.109942465022383E-5	0.0
3	1.109942465022383E-5	5.549712325111916E-5	3.329827395067149E-5	2.219884930044766E-5	1.109942465022383E-5	0.0
4	2.219884930044766E-5	5.549712325111916E-5	3.329827395067149E-5	3.329827395067149E-5	1.109942465022383E-5	0.0
5	3.329827395067149E-5	5.549712325111916E-5	3.329827395067149E-5	4.439769860089532E-5	1.109942465022383E-5	0.0
6	3.329827395067149E-5	6.659654790134299E-5	4.439769860089532E-5	6.659654790134299E-5	1.109942465022383E-5	0.0
7	4.439769860089532E-5	6.659654790134299E-5	5.549712325111916E-5	7.769597255156682E-5	1.109942465022383E-5	0.0
8	5.549712325111916E-5	6.659654790134299E-5	5.549712325111916E-5	8.879539720179064E-5	1.109942465022383E-5	0.0
9	5.549712325111916E-5	6.659654790134299E-5	5.549712325111916E-5	9.989482185201448E-5	1.109942465022383E-5	0.0
10-14	9.101528213183543E-5	6.659654790134299E-5	6.881643283138776E-5	1.3541298073273074E-4	1.109942465022383E-5	0.0
15-19	1.1543401636232784E-4	9.989482185201448E-5	9.32351670618802E-5	1.7759079440358129E-4	1.3319309580268597E-5	0.0
20-24	1.2209367115246215E-4	1.1543401636232784E-4	1.4873229031299933E-4	2.3974757244483473E-4	2.219884930044766E-5	0.0
25-29	1.2209367115246215E-4	1.4207263552286502E-4	1.7759079440358129E-4	2.996844655560434E-4	2.6638619160537194E-5	0.0
30-34	1.8425044919371557E-4	1.7315102454349175E-4	1.7759079440358129E-4	3.685008983874312E-4	6.881643283138776E-5	0.0
35-39	3.7738043810761027E-4	1.8869021905380514E-4	1.8647033412376034E-4	4.7949514488966947E-4	1.0433459171210402E-4	0.0
40-44	5.70510427021505E-4	1.8869021905380514E-4	2.0866918342420798E-4	5.394320380008782E-4	1.642714848233127E-4	2.885850409058196E-5
45-49	6.903842132439224E-4	2.0866918342420798E-4	2.3308791765470045E-4	6.126882406923555E-4	1.975697587739842E-4	9.32351670618802E-5
50-54	7.791796104457129E-4	2.3086803272465568E-4	2.464072272349691E-4	6.970438680340565E-4	2.3086803272465568E-4	1.1987378622241738E-4
55-59	8.590954679273245E-4	2.464072272349691E-4	2.619464217452824E-4	7.96938689886071E-4	2.5084699709505864E-4	1.3319309580268597E-4
60-64	9.390113254089361E-4	2.6638619160537194E-4	2.952446956959539E-4	8.74634662437638E-4	2.819253861156853E-4	1.7093113961344698E-4
65-69	9.85628908939876E-4	2.819253861156853E-4	3.152236600663568E-4	9.811891390797867E-4	2.9968446555604346E-4	1.8869021905380514E-4
70-74	0.0018513840316573347	2.9968446555604346E-4	3.3076285457667013E-4	0.0010877436157219354	3.218833148564911E-4	1.9978964370402896E-4
75-79	0.018995555346393064	3.107838902062673E-4	3.4186227922689395E-4	0.0011632197033434575	3.440821641569387E-4	2.1088906835425278E-4
80-84	0.026303416536100438	3.5074181894707303E-4	3.707207833174759E-4	0.0012098372868743976	4.8171502981971426E-4	2.1088906835425278E-4
85-89	0.026421070437392807	4.08458827128237E-4	4.040190572681474E-4	0.0012764338347757407	7.813994953757578E-4	2.1088906835425278E-4
90-94	0.026449928941483392	4.794951448896695E-4	4.239980216385503E-4	0.001320831533376636	7.991585748161158E-4	2.1088906835425278E-4
95-99	0.0264854471003641	5.261127284206097E-4	4.772752599596248E-4	0.0013896479662080237	8.124778843963844E-4	2.1088906835425278E-4
100-104	0.026523185144174864	5.571911174412363E-4	4.994741092600724E-4	0.001445145089459143	8.213574241165634E-4	2.1088906835425278E-4
105-109	0.02657646238249594	5.838297366017735E-4	5.549712325111915E-4	0.001527280831870799	8.213574241165634E-4	2.1088906835425278E-4
110-114	0.026600881116726437	5.88269506461863E-4	5.549712325111915E-4	0.001644934733163172	8.213574241165634E-4	2.1088906835425278E-4
115-119	0.02666525777969773	5.88269506461863E-4	5.88269506461863E-4	0.0017603687495254996	8.302369638367426E-4	2.1088906835425278E-4
120-124	0.026851728113821492	5.88269506461863E-4	5.971490461820422E-4	0.0018269652974268423	8.524358131371902E-4	2.1088906835425278E-4
125-129	0.027180271083468117	5.88269506461863E-4	6.060285859022212E-4	0.0018980016151882752	8.63535237787414E-4	2.219884930044766E-4
130-134	0.027433337965493222	5.993689311120868E-4	6.193478954824897E-4	0.0019779174726698868	8.968335117380856E-4	2.219884930044766E-4
135-139	0.02769750427216855	6.348870899928031E-4	6.37106974922848E-4	0.00204451402057123	9.345715555488467E-4	2.2642826286456614E-4
140-144	0.028154800567757772	6.437666297129822E-4	6.792847885936984E-4	0.002128869647912931	9.434510952690256E-4	2.441873423049243E-4
145-149	0.029051634079495854	6.437666297129822E-4	7.259023721246386E-4	0.002177707116373916	9.5011075005916E-4	2.441873423049243E-4
150-154	0.029777536451620495	6.593058242232955E-4	7.481012214250863E-4	0.0022265445848349006	9.589902897793389E-4	2.441873423049243E-4
155-159	0.02995734713095412	6.792847885936984E-4	7.88059150165892E-4	0.0022753820532958853	9.811891390797867E-4	2.441873423049243E-4
160-164	0.030128278270567566	6.881643283138775E-4	8.16917654256474E-4	0.0022998007875263777	9.989482185201446E-4	2.441873423049243E-4
165-167	0.030349526801928695	6.881643283138775E-4	8.324568487667873E-4	0.0023456784094139697	0.001017447259603851	2.5158695873840685E-4
>>END_MODULE
