##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293903
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9324920
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.98620299155382	41.0	40.0	41.0	38.0	41.0
2	40.07542037894159	41.0	40.0	41.0	38.0	41.0
3	40.10210532637277	41.0	40.0	41.0	38.0	41.0
4	40.11327496643403	41.0	40.0	41.0	38.0	41.0
5	40.10809100775127	41.0	40.0	41.0	38.0	41.0
6	40.12830994796738	41.0	40.0	41.0	38.0	41.0
7	40.116346842653876	41.0	40.0	41.0	38.0	41.0
8	40.09091466736444	41.0	40.0	41.0	38.0	41.0
9	40.079424166641644	41.0	40.0	41.0	38.0	41.0
10-14	40.01379010222071	41.0	40.0	41.0	38.0	41.0
15-19	39.8830244763494	41.0	40.0	41.0	38.0	41.0
20-24	39.71546571981314	40.6	39.8	41.0	37.4	41.0
25-29	39.6010945080494	40.0	39.0	41.0	36.8	41.0
30-34	39.35417249692223	40.0	38.8	41.0	35.6	41.0
35-39	39.16900608262591	40.0	38.4	41.0	35.0	41.0
40-44	38.66728021259164	40.0	36.8	41.0	35.0	41.0
45-49	38.0111797634725	39.0	35.4	41.0	34.8	41.0
50-54	37.29628309947967	37.2	35.0	40.0	34.0	41.0
55-59	36.57526183602648	36.0	35.0	39.0	34.0	41.0
60-64	35.60890010852639	35.0	34.4	36.8	32.8	39.6
65-69	35.43274297259387	35.0	35.0	36.2	33.0	38.8
70-74	35.1586435272367	35.0	35.0	35.8	33.0	37.0
75-79	34.98171501739425	35.0	35.0	35.0	33.0	36.6
80-84	34.903127983939804	35.0	35.0	35.0	33.0	36.0
85-89	35.66694390943836	36.2	35.6	36.2	33.2	37.0
90-94	39.79346563446474	41.0	40.0	41.0	37.2	41.0
95-99	39.74521687702067	41.0	40.0	41.0	37.0	41.0
100-104	39.62196037641634	41.0	39.6	41.0	36.8	41.0
105-109	39.45600480330914	40.2	39.0	41.0	36.0	41.0
110-114	39.240782506720755	40.0	38.4	41.0	35.2	41.0
115-119	38.94376173749313	40.0	38.0	41.0	35.0	41.0
120-124	38.65385976144277	40.0	37.4	41.0	34.8	41.0
125-129	37.880803355158356	39.0	36.0	40.2	33.8	40.6
130-134	38.04886664869123	39.0	35.2	41.0	34.0	41.0
135-139	37.59698546182029	38.0	35.0	40.8	34.0	41.0
140-144	36.903982162201714	36.6	35.0	39.4	34.0	41.0
145-149	36.1927617998755	35.4	35.0	38.0	33.8	40.6
150-154	35.562896817490376	35.0	35.0	36.6	33.0	39.0
155-159	35.082101940850684	35.0	35.0	35.8	33.0	37.0
160-164	34.762413616330306	35.0	35.0	35.0	33.0	36.2
165-169	34.58595948528556	35.0	35.0	35.0	33.0	36.0
170-174	34.4926778319227	35.0	34.6	35.0	33.0	35.8
175-176	33.9979526481449	35.0	33.5	35.0	31.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	8.0
29	151.0
30	1306.0
31	7621.0
32	31936.0
33	100599.0
34	249149.0
35	572579.0
36	1414425.0
37	2882751.0
38	3254227.0
39	803113.0
40	7055.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.6813634862283	27.845268377637556	28.485692102452354	20.98767603368179
2	20.191647756763597	26.405835117084113	30.418309218738603	22.984207907413683
3	21.061499723322022	25.512025840436163	30.88434002650961	22.5421344097322
4	22.517051084620565	26.27016639284841	28.30244120056794	22.910341321963084
5	22.619207457007672	26.816959287586386	27.903982018076295	22.65985123732965
6	22.342336448999024	27.210056493782254	27.91060942077787	22.536997636440848
7	22.619818722305393	27.14990584369625	27.474573508405438	22.755701925592927
8	22.84142920260978	27.36125350137052	27.315397880089055	22.481919415930644
9	22.300630997370487	27.49258974875924	27.66024802357554	22.546531230294736
10-14	22.704126147999123	27.375171047043835	27.30857101186927	22.61213179308777
15-19	22.450341665129567	27.391977625545312	27.579556714695673	22.578123994629447
20-24	22.46396966408291	27.281083376586608	27.6425792392857	22.612367720044784
25-29	22.5612745203176	27.320148591087108	27.412044285634625	22.706532602960667
30-34	22.49056935609099	27.331627509941104	27.455145995890582	22.722657138077324
35-39	22.50963439900825	27.385251562479894	27.480894206062896	22.624219832448965
40-44	22.566778052787583	27.378042921547852	27.376149071520185	22.67902995414438
45-49	22.59923516770117	27.33019907945591	27.342782565426837	22.727783187416083
50-54	22.626827897719227	27.386607070087464	27.365751127087417	22.620813905105887
55-59	22.653547697996338	27.356556410135425	27.271654877468116	22.718241014400125
60-64	22.525375016622124	27.395531543434153	27.382692827391548	22.69640061255217
65-69	22.57208855411092	27.39273044701724	27.368961878493327	22.66621912037851
70-74	22.57314379104593	27.42558649296723	27.304024055970455	22.697245660016385
75-79	22.48743796193426	27.396252193048305	27.37690403778263	22.739405807234807
80-84	22.522717621169942	27.449753992527548	27.34659171338735	22.680936672915156
85-89	22.497938856311904	27.50877004842937	27.491729687761396	22.50156140749733
90-94	21.711505263170768	26.589493616018945	29.032345665607107	22.666655455203184
95-99	22.51003911490669	27.41959166230432	27.52867136355809	22.5416978592309
100-104	22.436156031719857	27.422962353249215	27.519177858094707	22.62170375693622
105-109	22.356843397994737	27.399414866465538	27.723881128296473	22.51986060724325
110-114	22.401258754845912	27.31284712744554	27.59786220227498	22.68803191543357
115-119	22.34956486149159	27.411690439952856	27.532989503873956	22.705755194681597
120-124	22.375447131498657	27.42940822408599	27.528152107855075	22.666992536560276
125-129	22.371200120814493	27.45563236968011	27.493419652107633	22.67974785739776
130-134	22.50418852906965	27.36709992502374	27.403719708458308	22.724991837448297
135-139	22.54562930464029	27.448473061358815	27.41226105122348	22.593636582777414
140-144	22.55240080112893	27.46433429463148	27.3858519126913	22.59741299154829
145-149	22.435595972693957	27.52062834763331	27.454096375124248	22.589679304548486
150-154	22.412824391850698	27.597902722757606	27.50074091200132	22.488531973390373
155-159	22.380269299135875	27.64070064427645	27.48020971682317	22.498820339764507
160-164	22.258932906389845	27.723061968395836	27.578921229463244	22.43908389575108
165-169	22.25665315788721	27.775586041189438	27.608616175987127	22.359144624936224
170-174	22.213734254590133	27.86955882190251	27.623570745575808	22.29313617793155
175-176	22.12535111835328	27.918564003250445	27.52456495449592	22.43151992390036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.5
10	4.5
11	8.5
12	23.0
13	52.5
14	110.5
15	238.5
16	452.5
17	837.0
18	1523.0
19	2695.0
20	4610.0
21	7570.0
22	11722.0
23	17440.5
24	24921.0
25	34047.5
26	45315.0
27	58558.0
28	73615.5
29	90498.5
30	108989.0
31	129046.0
32	150490.0
33	173180.0
34	197235.5
35	222731.0
36	249790.5
37	277375.0
38	304016.0
39	329263.0
40	353008.5
41	373764.0
42	389983.0
43	400833.5
44	405453.5
45	404286.5
46	396670.0
47	382819.0
48	363879.0
49	341948.0
50	318122.0
51	291471.5
52	264716.5
53	240139.0
54	217208.5
55	196203.0
56	177422.5
57	161698.5
58	147928.0
59	134818.5
60	123131.0
61	112405.0
62	101718.0
63	91127.0
64	80768.5
65	70178.5
66	59665.5
67	49756.0
68	40606.5
69	32460.5
70	25132.0
71	18835.0
72	13757.0
73	9579.5
74	6549.0
75	4383.0
76	2803.5
77	1737.0
78	1024.5
79	599.0
80	330.0
81	157.5
82	67.5
83	31.0
84	17.0
85	8.0
86	2.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	828.0
92-93	2015.0
94-95	3030.0
96-97	4086.0
98-99	5026.0
100-101	6021.0
102-103	6927.0
104-105	7754.0
106-107	8656.0
108-109	9714.0
110-111	10663.0
112-113	11162.0
114-115	11866.0
116-117	12648.0
118-119	13336.0
120-121	13867.0
122-123	14631.0
124-125	15431.0
126-127	16038.0
128-129	16842.0
130-131	17687.0
132-133	53447.0
134-135	89409.0
136-137	84284.0
138-139	82188.0
140-141	83498.0
142-143	91999.0
144-145	88876.0
146-147	90677.0
148-149	91787.0
150-151	88974.0
152-153	88009.0
154-155	87990.0
156-157	88251.0
158-159	93887.0
160-161	91872.0
162-163	95080.0
164-165	98368.0
166-167	97592.0
168-169	102021.0
170-171	104667.0
172-173	111353.0
174-175	299025.0
176-177	6913438.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.73124098124502
#Duplication Level	Percentage of total
1	89.86429940262812
2	9.050996884463846
3	0.9041156808204825
4	0.11781149557060966
5	0.03063200567620662
6	0.01539804990690314
7	0.006192154514504572
8	0.005187670644146016
9	0.0010430617863290816
>10	0.004323593988855025
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1447905183100766E-5	0.0	1.0723952591550383E-5	0.0	0.0	0.0
2	3.217185777465115E-5	0.0	2.1447905183100766E-5	0.0	0.0	0.0
3	3.217185777465115E-5	1.0723952591550383E-5	2.1447905183100766E-5	0.0	0.0	0.0
4	3.217185777465115E-5	4.289581036620153E-5	2.1447905183100766E-5	0.0	0.0	0.0
5	3.217185777465115E-5	7.506766814085268E-5	2.1447905183100766E-5	0.0	0.0	0.0
6	3.217185777465115E-5	7.506766814085268E-5	3.217185777465115E-5	1.0723952591550383E-5	0.0	0.0
7	4.289581036620153E-5	1.0723952591550384E-4	4.289581036620153E-5	1.0723952591550383E-5	0.0	0.0
8	4.289581036620153E-5	1.1796347850705422E-4	4.289581036620153E-5	1.0723952591550383E-5	0.0	0.0
9	4.289581036620153E-5	1.286874310986046E-4	5.361976295775192E-5	1.0723952591550383E-5	0.0	0.0
10-14	5.790934399437206E-5	1.3512180265353485E-4	7.077808710423254E-5	1.0723952591550383E-5	2.1447905183100766E-6	0.0
15-19	6.43437155493023E-5	1.4155617420846507E-4	9.437078280564338E-5	2.3592695701410838E-5	1.0723952591550383E-5	0.0
20-24	7.721245865916277E-5	1.7372803198311622E-4	1.0938431643381389E-4	6.0054134512682146E-5	1.0723952591550383E-5	0.0
25-29	1.0938431643381392E-4	2.144790518310077E-4	1.5656970783663562E-4	1.0723952591550385E-4	2.1447905183100763E-5	0.0
30-34	2.1233426131269756E-4	2.294925854591782E-4	1.823071940563565E-4	1.587144983549457E-4	4.289581036620153E-5	2.1447905183100766E-6
35-39	3.560352260394727E-4	2.5523007167889915E-4	2.3163737597748827E-4	1.7158324146480613E-4	5.147497243944184E-5	1.286874310986046E-5
40-44	5.490663726873796E-4	2.680988147887596E-4	2.487957001239689E-4	2.016103087211472E-4	9.651557332395345E-5	3.0027067256341066E-5
45-49	8.4075788317755E-4	2.680988147887596E-4	2.874019294535503E-4	2.144790518310077E-4	1.5013533628170536E-4	3.217185777465115E-5
50-54	0.0010702504686367282	3.002706725634107E-4	3.324425303380619E-4	2.2734779494086813E-4	1.9517593716621699E-4	4.504060088451161E-5
55-59	0.0012740055678761857	3.34587320856372E-4	3.581800165577828E-4	2.445061190873487E-4	2.209134233859379E-4	6.0054134512682146E-5
60-64	0.0014198513231212708	4.096549889972247E-4	4.20378941588775E-4	2.680988147887596E-4	2.5523007167889915E-4	7.077808710423254E-5
65-69	0.001565697078366356	4.675643329915967E-4	5.168945149127285E-4	3.302977398197518E-4	2.9169151049017044E-4	8.150203969578292E-5
70-74	0.0062198925030992214	4.8257786661976725E-4	5.769486494254107E-4	3.64614388112713E-4	3.324425303380619E-4	1.1152910695212399E-4
75-79	0.0383123930285729	4.8257786661976725E-4	6.134100882366818E-4	3.689039691493332E-4	3.7104875966764326E-4	1.3512180265353485E-4
80-84	0.0454223735967708	4.868674476563873E-4	6.348579934197828E-4	4.1608936055215483E-4	5.748038589071006E-4	1.5013533628170536E-4
85-89	0.04553819228475955	5.04025771802868E-4	6.455819460113331E-4	4.5255079936342617E-4	7.699797960733175E-4	1.5013533628170536E-4
90-94	0.04570763073570604	5.233288864676586E-4	6.734642227493641E-4	4.890122381746975E-4	7.742693771099377E-4	1.5013533628170536E-4
95-99	0.04576339528918211	5.833830209803409E-4	7.120704520789453E-4	5.147497243944185E-4	7.849933297014881E-4	1.5013533628170536E-4
100-104	0.04579771193747507	6.005413451268214E-4	7.635454245183873E-4	5.361976295775192E-4	7.935724917747282E-4	1.5013533628170536E-4
105-109	0.045840607747841274	6.134100882366818E-4	8.042964443662787E-4	5.619351157972401E-4	7.935724917747282E-4	1.5013533628170536E-4
110-114	0.045885648348725785	6.348579934197827E-4	8.300339305859995E-4	5.85527811498651E-4	8.064412348845887E-4	1.5013533628170536E-4
115-119	0.04599503266515959	6.798985943042943E-4	8.772193219888213E-4	6.348579934197827E-4	8.450474642141702E-4	1.5013533628170536E-4
120-124	0.04632104082394273	7.185048236338756E-4	9.20115132355023E-4	6.541611080845734E-4	8.600609978423408E-4	1.5013533628170536E-4
125-129	0.046722116650866716	7.399527288169765E-4	9.630109427212245E-4	6.605954796395036E-4	8.922328556169919E-4	1.5013533628170536E-4
130-134	0.04719611535541323	7.978620728113485E-4	9.866036384226352E-4	6.927673374141548E-4	9.093911797634725E-4	1.5013533628170536E-4
135-139	0.04773016819447244	8.193099779944494E-4	0.001038078610862077	7.335183572620463E-4	9.308390849465734E-4	1.5013533628170536E-4
140-144	0.04809049300154854	8.386130926592401E-4	0.0010681056781184182	7.914277012564183E-4	9.694453142761546E-4	1.5228012680001545E-4
145-149	0.04828352414819644	8.557714168057207E-4	0.0011045671169296895	8.128756064395192E-4	0.0010209202867155964	1.6085928887325576E-4
150-154	0.04855805733454014	8.793641125071314E-4	0.0011431733462592708	8.343235116226198E-4	0.0010552369350085578	1.6085928887325576E-4
155-159	0.048862617588140166	9.522869901296742E-4	0.0011817795755888522	8.364683021409299E-4	0.0011110014884846199	1.7158324146480613E-4
160-164	0.049257259043509215	9.82314057386015E-4	0.0012160962238818135	8.471922547324803E-4	0.001147462927295891	1.8659677509297665E-4
165	0.04956610887814587	9.866036384226352E-4	0.0012225305954367436	8.471922547324803E-4	0.0011796347850705421	2.0375509923945727E-4
>>END_MODULE
