##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293904
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9342510
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.93381029295125	41.0	40.0	41.0	38.0	41.0
2	40.04994096875465	41.0	40.0	41.0	38.0	41.0
3	40.08783645936691	41.0	40.0	41.0	38.0	41.0
4	40.10173625717286	41.0	40.0	41.0	38.0	41.0
5	40.10066555989771	41.0	40.0	41.0	38.0	41.0
6	40.12315908679788	41.0	40.0	41.0	38.0	41.0
7	40.109602023439095	41.0	40.0	41.0	38.0	41.0
8	40.091726741528774	41.0	40.0	41.0	38.0	41.0
9	40.06646682743717	41.0	40.0	41.0	38.0	41.0
10-14	40.00588905979228	41.0	40.0	41.0	38.0	41.0
15-19	39.87624208055437	41.0	40.0	41.0	38.0	41.0
20-24	39.6966282508662	40.6	39.6	41.0	37.2	41.0
25-29	39.586466463509275	40.0	39.0	41.0	36.8	41.0
30-34	39.341700485201514	40.0	38.8	41.0	35.6	41.0
35-39	39.157291841271785	40.0	38.2	41.0	35.0	41.0
40-44	38.65623411695573	40.0	36.8	41.0	35.0	41.0
45-49	38.0009341814994	39.0	35.4	41.0	34.8	41.0
50-54	37.29774628017525	37.2	35.0	40.0	34.0	41.0
55-59	36.588361072131576	36.2	35.0	39.0	34.0	41.0
60-64	35.63348620445683	35.0	34.4	37.0	33.2	39.6
65-69	35.459134108499754	35.0	35.0	36.2	33.0	39.0
70-74	35.2002492906082	35.0	35.0	35.8	33.0	37.2
75-79	35.030916702256675	35.0	35.0	35.0	33.0	37.0
80-84	34.95799383677406	35.0	35.0	35.0	33.0	36.0
85-89	35.714980813507296	36.2	35.6	36.2	33.2	37.2
90-94	39.809472545116066	41.0	40.0	41.0	37.6	41.0
95-99	39.75572743564582	41.0	40.0	41.0	37.0	41.0
100-104	39.63165674960287	41.0	39.4	41.0	36.8	41.0
105-109	39.45750444506589	40.2	39.0	41.0	36.0	41.0
110-114	39.24946079126612	40.0	38.4	41.0	35.2	41.0
115-119	38.95798727862699	40.0	38.0	41.0	35.0	41.0
120-124	38.673017769446616	40.0	37.4	41.0	35.0	41.0
125-129	37.91775525700801	39.0	36.0	40.2	34.0	40.6
130-134	38.07359017760001	39.0	35.2	41.0	34.0	41.0
135-139	37.636106145102325	38.2	35.0	40.8	34.0	41.0
140-144	36.95734159982693	36.6	35.0	39.6	34.0	41.0
145-149	36.252749341681415	35.4	35.0	38.2	34.0	40.6
150-154	35.62270812724856	35.0	35.0	36.6	33.4	39.0
155-159	35.131680661679354	35.0	35.0	36.0	33.0	37.2
160-164	34.80223269136427	35.0	35.0	35.0	33.0	36.2
165-169	34.62169694251253	35.0	35.0	35.0	33.0	36.0
170-174	34.521187538203364	35.0	35.0	35.0	33.0	35.8
175-176	34.018885409180925	35.0	33.5	35.0	31.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	7.0
29	94.0
30	900.0
31	5734.0
32	26930.0
33	91648.0
34	242777.0
35	569621.0
36	1412005.0
37	2867658.0
38	3276868.0
39	840115.0
40	8153.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.656149150495956	27.842817401319348	28.50206207967666	20.998971368508034
2	20.19634980321134	26.440635332474894	30.37625862856984	22.986756235743925
3	21.073790662252435	25.51753222635031	30.868674478271902	22.540002633125358
4	22.501950760555783	26.258446605890708	28.295051329888864	22.94455130366465
5	22.646226763471486	26.837530813453768	27.87654495419325	22.63969746888149
6	22.304701841368114	27.226922957534967	27.89172288817459	22.57665231292233
7	22.594399149693174	27.193430887416763	27.449143752588974	22.763026210301085
8	22.84075692720693	27.359938603223334	27.308089581921774	22.491214887647967
9	22.30792367361662	27.49140220347637	27.638857223594087	22.561816899312927
10-14	22.709062125702836	27.380378506418513	27.28903902698525	22.6215203408934
15-19	22.464378416506914	27.38235013930946	27.574666765141277	22.578604679042353
20-24	22.474696842711435	27.264236270552562	27.64799395451544	22.61307293222057
25-29	22.569303110192017	27.30192849673161	27.417197305649125	22.711571087427256
30-34	22.50122504551775	27.3439514648633	27.44932571653656	22.705497773082396
35-39	22.497637144621734	27.385445667170817	27.48400162269026	22.63291556551719
40-44	22.587163406836062	27.358155356536944	27.379158277593497	22.675522959033493
45-49	22.60582862635416	27.323421650070483	27.35739967096637	22.713350052608988
50-54	22.631768122271207	27.389971217584996	27.368146247635806	22.610114412507986
55-59	22.64358293435062	27.35742964149891	27.288163459284497	22.710823964865973
60-64	22.522394945255613	27.38550988974055	27.382842512344112	22.709252652659725
65-69	22.56778531679388	27.39873117609722	27.362595276858148	22.670888230250757
70-74	22.569029093894464	27.42054758303711	27.315068434499935	22.695354888568488
75-79	22.500426544900677	27.400831254127638	27.364089521980713	22.734652678990976
80-84	22.511121743514323	27.450453893011623	27.359801595074558	22.6786227683995
85-89	22.510111308417116	27.5223895933748	27.470228022233854	22.49727107597423
90-94	21.73034963528148	26.588846232603686	29.02135213182605	22.659452000288784
95-99	22.524448072888877	27.408568557243353	27.529494324841487	22.537489045026284
100-104	22.451785336424244	27.431157319319215	27.51308351084902	22.603973833407515
105-109	22.38367461719655	27.3942087122915	27.71040361356753	22.511713056944416
110-114	22.41711104886174	27.293117045705717	27.601656854915124	22.688115050517418
115-119	22.356843584939394	27.40589090685923	27.539007326401922	22.698258181799453
120-124	22.375106741524096	27.424309671887272	27.53948157415749	22.661102012431144
125-129	22.388735450404777	27.450679598932314	27.488475459047446	22.672109491615462
130-134	22.501326992732753	27.37306346283773	27.408376061511902	22.717233482917614
135-139	22.557348479201103	27.460109671412248	27.39896358526351	22.58357826412314
140-144	22.553345334096285	27.45806079921809	27.39640378842664	22.59219007825899
145-149	22.423520900829452	27.505975277076537	27.469808520985882	22.60069530110812
150-154	22.415754716210003	27.59890872287497	27.51076072796535	22.47457583294968
155-159	22.39310474595747	27.64247103249889	27.480229996002674	22.484194225540968
160-164	22.27988499509924	27.713839720886103	27.56575385539052	22.44052142862414
165-169	22.26636474510782	27.772818995757042	27.60863074825532	22.35218551087982
170-174	22.218202839996263	27.86792605507655	27.61364614331398	22.30022496161321
175-176	22.11900514902862	27.92142640506671	27.544854902925387	22.414713542979282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.5
10	2.5
11	8.5
12	29.5
13	56.5
14	117.5
15	235.0
16	452.0
17	867.0
18	1570.5
19	2823.0
20	4674.0
21	7487.0
22	11678.5
23	17472.0
24	24812.0
25	33833.5
26	45122.0
27	58512.0
28	73749.5
29	90458.5
30	108901.5
31	128939.5
32	150358.0
33	173212.5
34	197632.0
35	223403.5
36	249974.0
37	277623.5
38	304486.0
39	330347.0
40	354221.0
41	374183.5
42	390737.5
43	402834.0
44	407957.0
45	405990.0
46	397686.5
47	383547.5
48	364405.0
49	341812.5
50	317530.0
51	291421.5
52	264932.0
53	240190.0
54	217489.0
55	196958.0
56	178506.0
57	161953.5
58	147824.0
59	135402.5
60	123763.5
61	112598.5
62	101998.0
63	91404.5
64	80453.0
65	70024.0
66	59992.5
67	49946.0
68	40458.5
69	32377.0
70	25364.5
71	18959.0
72	13790.5
73	9845.5
74	6750.5
75	4408.0
76	2785.0
77	1691.5
78	1007.0
79	596.5
80	315.0
81	150.0
82	71.0
83	38.5
84	21.0
85	9.0
86	4.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	867.0
92-93	2456.0
94-95	3250.0
96-97	4267.0
98-99	5856.0
100-101	6150.0
102-103	7221.0
104-105	8065.0
106-107	9074.0
108-109	10156.0
110-111	11080.0
112-113	12102.0
114-115	12926.0
116-117	13777.0
118-119	13886.0
120-121	14630.0
122-123	15301.0
124-125	16299.0
126-127	16932.0
128-129	17582.0
130-131	18225.0
132-133	55989.0
134-135	93097.0
136-137	90984.0
138-139	85848.0
140-141	95814.0
142-143	89285.0
144-145	89406.0
146-147	90126.0
148-149	90508.0
150-151	96881.0
152-153	91258.0
154-155	97742.0
156-157	97215.0
158-159	108859.0
160-161	98492.0
162-163	98621.0
164-165	102117.0
166-167	99449.0
168-169	108189.0
170-171	106021.0
172-173	113725.0
174-175	298693.0
176-177	6824089.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.76736347098677
#Duplication Level	Percentage of total
1	89.93669869761808
2	8.986649291254553
3	0.8758179974424469
4	0.13812053174289843
5	0.033694082820873474
6	0.015397390520666175
7	0.007223802660136899
8	0.0
9	0.002085975333508097
>10	0.004312230606806945
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1407523245894306E-5	0.0	0.0	1.0703761622947153E-5	0.0	0.0
2	2.1407523245894306E-5	0.0	0.0	1.0703761622947153E-5	0.0	0.0
3	4.281504649178861E-5	3.211128486884146E-5	1.0703761622947153E-5	1.0703761622947153E-5	0.0	0.0
4	4.281504649178861E-5	6.422256973768292E-5	3.211128486884146E-5	1.0703761622947153E-5	0.0	0.0
5	4.281504649178861E-5	6.422256973768292E-5	5.3518808114735765E-5	1.0703761622947153E-5	0.0	0.0
6	4.281504649178861E-5	7.492633136063006E-5	5.3518808114735765E-5	2.1407523245894306E-5	0.0	0.0
7	4.281504649178861E-5	8.563009298357722E-5	6.422256973768292E-5	4.281504649178861E-5	0.0	0.0
8	4.281504649178861E-5	9.633385460652437E-5	6.422256973768292E-5	6.422256973768292E-5	0.0	0.0
9	4.281504649178861E-5	9.633385460652437E-5	7.492633136063006E-5	7.492633136063006E-5	0.0	0.0
10-14	4.9237303465556906E-5	1.2202288250159755E-4	1.1345987320323982E-4	9.419310228193494E-5	6.422256973768292E-6	0.0
15-19	5.994106508850406E-5	1.626971766687967E-4	1.3914890109831298E-4	1.0703761622947153E-4	1.9266770921304877E-5	0.0
20-24	7.06448267114512E-5	2.033714708359959E-4	1.5627491969502841E-4	1.2844513947536583E-4	2.997053254425203E-5	0.0
25-29	7.706708368521949E-5	2.3334200338024792E-4	2.033714708359959E-4	1.3058589179995526E-4	3.639278951802032E-5	0.0
30-34	1.9266770921304877E-4	2.675940405736788E-4	2.526087743015528E-4	1.4343040574749184E-4	6.208181741309349E-5	1.2844513947536584E-5
35-39	4.7738776838344304E-4	2.7615704987203653E-4	3.1254983939005683E-4	1.7126018596715445E-4	1.0061535925570324E-4	4.495579881637804E-5
40-44	7.578263229046583E-4	2.954238207933414E-4	3.6178714285561376E-4	1.9908996618681705E-4	1.3914890109831298E-4	6.208181741309349E-5
45-49	0.0010082943448816218	3.189720963638251E-4	4.003206846982235E-4	2.3548275570483735E-4	1.7982319526551214E-4	8.991159763275608E-5
50-54	0.0012459178529110485	3.425203719343089E-4	4.281504649178861E-4	2.6973479289826825E-4	2.247789940818902E-4	1.0917836855406096E-4
55-59	0.0014107557819044347	3.6820939982938204E-4	4.4741723583919103E-4	3.104090870654674E-4	2.654532882490894E-4	1.327266441245447E-4
60-64	0.0015991419864683043	3.8961692307527634E-4	5.137805579014632E-4	3.446611242588983E-4	3.018460777671097E-4	1.5627491969502841E-4
65-69	0.001725446373619081	4.0246143702281286E-4	5.565956043932519E-4	3.5536488588184547E-4	3.4680187658348775E-4	1.7126018596715445E-4
70-74	0.00646721277258467	4.281504649178861E-4	5.780031276391462E-4	4.1530595097034954E-4	3.960391800490446E-4	1.819639475901016E-4
75-79	0.03879471362620966	4.6454325443590647E-4	6.015514032096299E-4	4.409949788654227E-4	4.281504649178861E-4	1.9266770921304874E-4
80-84	0.0458955890868728	5.094990532522845E-4	6.507887066751867E-4	4.688247590850853E-4	6.143959171571666E-4	2.033714708359959E-4
85-89	0.045964093161259656	5.694401183407885E-4	6.721962299210812E-4	5.073583009276952E-4	7.70670836852195E-4	2.033714708359959E-4
90-94	0.04612036808095469	6.186774218063455E-4	6.850407438686178E-4	5.202028148752316E-4	7.70670836852195E-4	2.033714708359959E-4
95-99	0.04619315365999073	6.486479543505975E-4	7.021667624653332E-4	5.801438799637356E-4	7.728115891767844E-4	2.033714708359959E-4
100-104	0.04624881322043005	6.850407438686178E-4	7.428410566325324E-4	6.272404311047031E-4	7.813745984751421E-4	2.033714708359959E-4
105-109	0.04630661353319397	6.850407438686178E-4	7.813745984751421E-4	6.507887066751867E-4	7.899376077734998E-4	2.033714708359959E-4
110-114	0.04637297685525625	7.364187996587641E-4	8.241896449669307E-4	6.529294589997762E-4	8.027821217210364E-4	2.033714708359959E-4
115-119	0.04645218469126605	7.685300845276055E-4	8.798492054062558E-4	6.700554775964918E-4	8.241896449669307E-4	2.097937278097642E-4
120-124	0.04673048249346268	7.728115891767844E-4	9.483532797931177E-4	7.171520287374592E-4	8.712861961078982E-4	2.1407523245894306E-4
125-129	0.0472314185374166	8.00641369396447E-4	0.0010040128402324432	7.535448182554795E-4	9.162419949242763E-4	2.312012510556585E-4
130-134	0.04779657715110821	8.520194251865933E-4	0.0010361241251012842	7.835153507997315E-4	9.3979027049476E-4	2.3548275570483735E-4
135-139	0.04831249846133427	8.926937193537924E-4	0.001063953905320947	7.96359864747268E-4	9.868868216357275E-4	2.3548275570483735E-4
140-144	0.04870853764138331	9.569162890914754E-4	0.0010875021808914305	8.306119019406991E-4	0.0010125758495308009	2.3548275570483735E-4
145-149	0.04891190911221931	0.0010168573541799795	0.0011024874471635567	8.584416821603616E-4	0.001029701868127516	2.3548275570483735E-4
150-154	0.0491773624004684	0.0010254203634783372	0.0011388802366815768	8.75567700757077E-4	0.0010468278867242313	2.3548275570483735E-4
155-159	0.04956697932354367	0.0010404056297504633	0.0011902582924717233	8.884122147046137E-4	0.0010832206762422518	2.3548275570483735E-4
160-164	0.0499865667791632	0.001048968639048821	0.001198821301770081	9.012567286521504E-4	0.0011024874471635567	2.3548275570483735E-4
165	0.050222049534868037	0.001048968639048821	0.001198821301770081	9.205234995734551E-4	0.0011024874471635567	2.461865173277845E-4
>>END_MODULE
