##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293918
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8529553
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.40669833460206	39.0	39.0	39.0	37.0	39.0
2	40.19775385650338	41.0	40.0	41.0	38.0	41.0
3	40.235792895594884	41.0	40.0	41.0	38.0	41.0
4	40.242364869530675	41.0	40.0	41.0	38.0	41.0
5	40.23686505025527	41.0	40.0	41.0	38.0	41.0
6	40.22334628790043	41.0	40.0	41.0	38.0	41.0
7	40.24546268720061	41.0	40.0	41.0	38.0	41.0
8	40.22846191353756	41.0	40.0	41.0	38.0	41.0
9	40.21026822859299	41.0	40.0	41.0	38.0	41.0
10-14	40.15334977108414	41.0	40.0	41.0	38.0	41.0
15-19	40.02969726549562	41.0	40.0	41.0	38.0	41.0
20-24	39.85126432768517	41.0	40.0	41.0	37.8	41.0
25-29	39.600808154893926	40.8	39.2	41.0	36.6	41.0
30-34	39.413587511561275	40.4	39.0	41.0	35.6	41.0
35-39	39.18814313012651	40.4	38.0	41.0	35.0	41.0
40-44	38.60226504249402	39.8	36.6	41.0	35.0	41.0
45-49	37.88569234519089	38.6	35.0	41.0	35.0	41.0
50-54	37.14976222083385	36.8	35.0	39.8	34.2	41.0
55-59	36.45184982143847	35.6	35.0	38.8	34.0	41.0
60-64	35.733548241039124	35.0	34.8	36.8	33.6	39.6
65-69	35.40418826168265	35.0	35.0	36.0	33.6	38.8
70-74	35.22677106291502	35.0	35.0	35.6	34.0	37.2
75-79	35.077815730789176	35.0	35.0	35.0	33.0	37.0
80-84	35.01944249599012	35.0	35.0	35.0	33.0	36.0
85-89	34.838893175293	35.0	34.6	35.0	32.6	36.2
90-94	39.65842371353499	40.6	39.8	40.6	37.8	40.6
95-99	39.92109578475316	41.0	40.0	41.0	38.0	41.0
100-104	39.8038427241799	41.0	40.0	41.0	37.0	41.0
105-109	39.648266832149616	41.0	39.6	41.0	36.4	41.0
110-114	39.46015149328754	40.8	39.0	41.0	35.6	41.0
115-119	39.16698058310577	40.0	38.2	41.0	35.0	41.0
120-124	38.87022658797456	40.0	37.8	41.0	35.0	41.0
125-129	38.30393457273039	39.6	36.6	40.6	34.6	41.0
130-134	38.088174710608705	39.0	35.2	41.0	34.6	41.0
135-139	37.621923049955925	38.0	35.0	41.0	34.4	41.0
140-144	36.93991331309866	36.4	35.0	39.6	34.0	41.0
145-149	36.26137331915696	35.2	35.0	38.2	34.0	40.8
150-154	35.654660641099966	35.0	35.0	36.6	34.0	39.0
155-159	35.19180783636997	35.0	35.0	35.8	33.6	37.2
160-164	34.878589511184	35.0	35.0	35.0	33.0	36.2
165-169	34.68802064339091	35.0	35.0	35.0	33.0	36.0
170-174	34.58067368505282	35.0	35.0	35.0	33.0	35.6
175-178	34.28602988537094	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	36.0
30	407.0
31	3489.0
32	18577.0
33	68737.0
34	196507.0
35	484557.0
36	1328192.0
37	2859992.0
38	2866935.0
39	693617.0
40	8506.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.022847738914336	26.59421894676075	26.86629651049709	23.51663680382782
2	23.935263665047863	27.0541492619836	26.612297268098338	22.398289804870195
3	21.83874113918983	26.205464694339785	27.86500066298902	24.09079350348137
4	22.699771019653667	25.143498141110094	28.164852249584477	23.991878589651765
5	23.528993840591646	25.85841251001078	26.78387718559226	23.828716463805314
6	23.365186897836267	25.897324279478656	26.800501737898806	23.93698708478627
7	23.617122726126446	25.96141908022613	26.28966605870202	24.131792134945407
8	23.78040209141089	26.280415867044848	26.14766565141221	23.79151639013205
9	23.620792320535436	26.29265566437069	26.278340728992482	23.808211286101393
10-14	23.76418084277101	26.29625022553937	26.02775080945039	23.911818122239232
15-19	23.63732073650284	26.303505001962	26.209396905089864	23.84977735644529
20-24	23.593930420503863	26.257451005931966	26.340184532530603	23.808434041033568
25-29	23.63335335392136	26.233327819171766	26.15932394112564	23.973994885781234
30-34	23.639628008642422	26.283707950463526	26.196029264370598	23.88063477652346
35-39	23.6436305630553	26.313829106871133	26.199811408640056	23.842728921433515
40-44	23.678875082902938	26.308030444268297	26.16865151081188	23.844442962016885
45-49	23.76607777687764	26.22073864832073	26.12540422692725	23.887779347874385
50-54	23.730125130824558	26.314166756452533	26.151597862162294	23.80411025056061
55-59	23.741117500530216	26.25281066897644	26.098307848019704	23.907763982473643
60-64	23.66633984219337	26.283930705395697	26.18413415099244	23.86559530141849
65-69	23.67177740732721	26.311957965440865	26.16684602346688	23.849418603765052
70-74	23.656236147427656	26.304736016060865	26.149984647495593	23.889043189015887
75-79	23.62325903831068	26.295682786659512	26.195131210275612	23.88592696475419
80-84	23.61877580220206	26.337478646301864	26.184084910428485	23.85966064106759
85-89	23.64325539685374	26.30451091633993	26.10671391572337	23.945519771082964
90-94	23.05960128512279	26.15785493560116	27.202706073621847	23.579837705654203
95-99	23.54649749145559	26.163836085525393	26.38591187715652	23.903754545862494
100-104	23.69394714279254	26.311910439725807	26.153393726294066	23.840748691187592
105-109	23.587716644517148	26.316022816635854	26.293318125886323	23.80294241296068
110-114	23.585348514197662	26.17480765149128	26.385609502240293	23.85423433207076
115-119	23.59753173769155	26.29073818940127	26.252040466856112	23.859689606051067
120-124	23.60518297163409	26.285004132367217	26.24840578349109	23.861407112507603
125-129	23.571708165381317	26.26833796975519	26.286431070699706	23.873522794163783
130-134	23.690110969781482	26.300909594279876	26.233458127794812	23.77552130814383
135-139	23.76667408868233	26.242989502640167	26.161167516097727	23.829168892579773
140-144	23.700884902041647	26.308893960187735	26.2604146007612	23.729806537009416
145-149	23.68937566782132	26.350932027326067	26.257652386313996	23.702039918538617
150-154	23.640324363705126	26.418843924544184	26.268502306370223	23.67232940538047
155-159	23.588484918900292	26.42867264155704	26.32157605996414	23.66126637957853
160-164	23.55662725629273	26.55160646890761	26.353024499018762	23.538741775780895
165-169	23.508308521445844	26.566014606239957	26.363007602448146	23.562669269866053
170-174	23.44655655912226	26.619017252145504	26.449043537338902	23.485382651393333
175-178	23.443635936975475	26.717610633608334	26.391565246983323	23.44718818243287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	1.5
12	3.5
13	9.0
14	23.0
15	41.5
16	81.0
17	183.5
18	383.0
19	739.5
20	1430.5
21	2636.0
22	4386.0
23	7016.5
24	10897.5
25	16051.0
26	22633.0
27	31016.0
28	40708.0
29	51863.0
30	65175.5
31	80009.0
32	95924.5
33	113255.0
34	132160.5
35	153039.0
36	175819.0
37	198968.0
38	222624.0
39	246167.0
40	269483.0
41	291287.5
42	309363.0
43	325334.0
44	337278.0
45	344387.0
46	347637.0
47	346467.0
48	340819.0
49	332027.5
50	320733.5
51	307638.5
52	293211.5
53	277452.0
54	261171.0
55	244879.0
56	229334.5
57	213506.5
58	197150.5
59	181528.0
60	166025.5
61	149687.0
62	133846.0
63	118495.0
64	102967.0
65	88066.0
66	74124.5
67	61206.5
68	49565.0
69	39075.5
70	30179.0
71	22738.5
72	16495.0
73	11688.5
74	8085.0
75	5377.5
76	3477.0
77	2172.5
78	1303.0
79	763.5
80	427.0
81	244.5
82	140.5
83	71.0
84	32.0
85	14.5
86	7.0
87	2.5
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	827.0
92-93	1997.0
94-95	3028.0
96-97	3928.0
98-99	5095.0
100-101	5775.0
102-103	6836.0
104-105	7802.0
106-107	8615.0
108-109	9489.0
110-111	10277.0
112-113	11124.0
114-115	11823.0
116-117	12382.0
118-119	13085.0
120-121	13693.0
122-123	14283.0
124-125	14871.0
126-127	15608.0
128-129	16261.0
130-131	16717.0
132-133	18201.0
134-135	72405.0
136-137	81150.0
138-139	76564.0
140-141	74316.0
142-143	81668.0
144-145	80795.0
146-147	78324.0
148-149	80396.0
150-151	81075.0
152-153	76271.0
154-155	83134.0
156-157	79685.0
158-159	88173.0
160-161	84853.0
162-163	84234.0
164-165	86694.0
166-167	86001.0
168-169	86828.0
170-171	90306.0
172-173	92064.0
174-175	94603.0
176-177	257427.0
178-179	6310870.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.44810699781006
#Duplication Level	Percentage of total
1	91.41618580664058
2	7.259127819924563
3	0.9879467550503448
4	0.20648183107115922
5	0.059341098433541545
6	0.029844363519829487
7	0.010351326636313713
8	0.007288206962211975
9	0.010472352572431069
>10	0.012960439189031706
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.055154941882652E-4	0.0	0.0	4.6895775194784534E-5	0.0	0.0
2	0.0012075662112657017	1.1723943798696134E-5	0.0	5.861971899348067E-5	0.0	0.0
3	0.001231014098863094	3.51718313960884E-5	0.0	9.379155038956907E-5	0.0	0.0
4	0.001231014098863094	3.51718313960884E-5	1.1723943798696134E-5	1.2896338178565746E-4	0.0	0.0
5	0.001231014098863094	3.51718313960884E-5	2.3447887597392267E-5	1.406873255843536E-4	0.0	0.0
6	0.00124273804266179	3.51718313960884E-5	2.3447887597392267E-5	1.5241126938304972E-4	0.0	0.0
7	0.00124273804266179	4.6895775194784534E-5	2.3447887597392267E-5	1.8758310077913814E-4	0.0	0.0
8	0.00124273804266179	4.6895775194784534E-5	2.3447887597392267E-5	1.8758310077913814E-4	0.0	0.0
9	0.0012544619864604863	5.861971899348067E-5	2.3447887597392267E-5	1.8758310077913814E-4	0.0	0.0
10-14	0.0012685307190189218	7.503324031165526E-5	3.048225387660995E-5	2.0634141085705193E-4	0.0	0.0
15-19	0.0013083921279344883	9.848112790904752E-5	4.9240563954523755E-5	2.2744450969470502E-4	7.03436627921768E-6	0.0
20-24	0.001352943114369534	1.1254986046748288E-4	7.03436627921768E-5	2.6730591861027185E-4	2.5792676357131492E-5	0.0
25-29	0.0013834253682461436	1.24273804266179E-4	9.144676162982983E-5	3.118569050453172E-4	4.6895775194784534E-5	0.0
30-34	0.001467837763596756	1.336529593051359E-4	1.1958422674670054E-4	3.7047662403879786E-4	4.6895775194784534E-5	4.689577519478454E-6
35-39	0.0016366625542979802	2.180653546557481E-4	1.406873255843536E-4	4.3847549807123537E-4	5.158535271426299E-5	1.406873255843536E-5
40-44	0.0018430039651550322	2.555819748115757E-4	1.4772169186357125E-4	4.6661296318810613E-4	6.09645077532199E-5	3.51718313960884E-5
45-49	0.0021689296027587847	3.329600038829702E-4	1.8758310077913814E-4	5.18198315902369E-4	9.144676162982983E-5	6.565408527269833E-5
50-54	0.002440925098888535	3.681318352790586E-4	1.9930704457783426E-4	5.393014147400221E-4	1.2896338178565746E-4	9.144676162982983E-5
55-59	0.002680093552381936	3.8923493411671163E-4	2.1337577713626964E-4	5.604045135776751E-4	1.3365295930513593E-4	1.0082591666878676E-4
60-64	0.002858297498122117	4.267515542725393E-4	2.2744450969470502E-4	5.885419786945459E-4	1.4772169186357125E-4	1.313081705453967E-4
65-69	0.002989605668667514	4.713025407075846E-4	2.4151324225314035E-4	6.166794438114166E-4	1.7116957946096355E-4	1.6179042442200663E-4
70-74	0.004633302589244712	5.322670484608045E-4	2.9778817248688177E-4	6.682647965256796E-4	1.9930704457783426E-4	2.0868619961679117E-4
75-79	0.036114436477503564	5.979211337335029E-4	3.51718313960884E-4	6.987470504022895E-4	2.3682366473366188E-4	2.3213408721418346E-4
80-84	0.049315597194835414	6.166794438114165E-4	3.6109746899984086E-4	7.76125079473684E-4	3.282704263634918E-4	2.3447887597392267E-4
85-89	0.04937656170258864	6.42472120168548E-4	3.751662015582763E-4	8.394343759866432E-4	5.369566259802829E-4	2.462028197726188E-4
90-94	0.04961104057856256	6.870231066035934E-4	4.009588779154078E-4	8.7929578490221E-4	5.557149360581966E-4	2.462028197726188E-4
95-99	0.049732969594069	7.081262054412465E-4	4.103380329543647E-4	9.097780387788199E-4	5.721284573763712E-4	2.462028197726188E-4
100-104	0.04985020903205596	7.245397267594209E-4	4.361307093114961E-4	9.379155038956907E-4	5.744732461361106E-4	2.462028197726188E-4
105-109	0.04990413917352996	7.550219806360311E-4	4.5957859690888846E-4	9.637081802528222E-4	5.744732461361106E-4	2.462028197726188E-4
110-114	0.04994869015996501	7.925386007918585E-4	5.017847945841946E-4	9.801217015709966E-4	5.744732461361106E-4	2.462028197726188E-4
115-119	0.05000027551267927	7.995729670710763E-4	5.439909922595006E-4	0.0010293622655255207	5.744732461361106E-4	2.462028197726188E-4
120-124	0.0502769605863285	8.183312771489901E-4	5.627493023374144E-4	0.001064534096921609	5.838524011750675E-4	2.48547608532358E-4
125-129	0.05085612340998409	8.300552209476863E-4	5.627493023374144E-4	0.0010786028294800442	6.401273314088089E-4	2.673059186102718E-4
130-134	0.0513532186270488	8.535031085450786E-4	5.627493023374144E-4	0.0011114298721163936	6.752991628048974E-4	2.6965070737001106E-4
135-139	0.05188314088674987	8.675718411035139E-4	5.955763449737635E-4	0.001139567337233264	6.940574728828111E-4	2.6965070737001106E-4
140-144	0.05245526934412624	8.957093062203845E-4	6.30748176369852E-4	0.001198187056226745	7.245397267594209E-4	2.6965070737001106E-4
145-149	0.05284684906700269	9.027436724996024E-4	6.61230430246462E-4	0.0012403932539020509	7.268845155191603E-4	2.6965070737001106E-4
150-154	0.05304381132282079	9.332259263762123E-4	7.034366279217681E-4	0.0012568067752202255	7.45642825597074E-4	2.813746511687072E-4
155-159	0.05332753076274923	9.543290252138654E-4	7.151605717204641E-4	0.0012919786066163137	7.64401135674988E-4	2.9309859496740335E-4
160-164	0.053775385415859425	0.0010176383217268242	7.245397267594209E-4	0.0013013577616552707	7.737802907139448E-4	2.9309859496740335E-4
165-167	0.05418806823757353	0.0010317070542852596	7.425164405840883E-4	0.0013013577616552707	7.815962532464089E-4	2.9309859496740335E-4
>>END_MODULE
