##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293919
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8372675
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.30655614842329	39.0	39.0	39.0	37.0	39.0
2	40.13027628565542	41.0	40.0	41.0	38.0	41.0
3	40.18826277145595	41.0	40.0	41.0	38.0	41.0
4	40.21314836656146	41.0	40.0	41.0	38.0	41.0
5	40.20671004189223	41.0	40.0	41.0	38.0	41.0
6	40.196175654734	41.0	40.0	41.0	38.0	41.0
7	40.21475884349984	41.0	40.0	41.0	38.0	41.0
8	40.205424550696165	41.0	40.0	41.0	38.0	41.0
9	40.16407217526059	41.0	40.0	41.0	38.0	41.0
10-14	40.128530200921446	41.0	40.0	41.0	38.0	41.0
15-19	40.005301531469925	41.0	40.0	41.0	38.0	41.0
20-24	39.82161089496487	41.0	40.0	41.0	37.6	41.0
25-29	39.55904028282479	40.6	39.0	41.0	36.6	41.0
30-34	39.3086857903836	40.0	38.6	41.0	35.2	41.0
35-39	39.119455180094775	40.0	37.8	41.0	35.0	41.0
40-44	38.59410004568433	39.8	36.6	41.0	35.0	41.0
45-49	37.8849359852138	38.6	35.0	41.0	35.0	41.0
50-54	37.157678663031824	36.8	35.0	39.8	34.2	41.0
55-59	36.469634280561465	35.6	35.0	38.8	34.0	41.0
60-64	35.75495683279239	35.0	34.8	36.8	33.6	39.6
65-69	35.45859869157706	35.0	35.0	36.0	33.6	39.0
70-74	35.29074230159418	35.0	35.0	35.8	34.0	37.4
75-79	35.14841720238753	35.0	35.0	35.0	33.2	37.0
80-84	35.12126633363889	35.0	35.0	35.0	33.0	37.0
85-89	34.966504301194064	35.0	34.6	35.0	32.6	38.2
90-94	39.59693061699078	40.6	39.8	40.6	37.8	40.6
95-99	39.865974130538504	41.0	40.0	41.0	37.6	41.0
100-104	39.73723289776795	41.0	40.0	41.0	36.8	41.0
105-109	39.57389378666881	41.0	39.2	41.0	36.0	41.0
110-114	39.37343990861082	40.6	39.0	41.0	35.2	41.0
115-119	39.07788832935268	40.0	38.0	41.0	35.0	41.0
120-124	38.770614486796966	40.0	37.4	41.0	35.0	41.0
125-129	38.19162499611825	39.6	36.0	40.6	34.2	41.0
130-134	37.846860264829076	39.0	35.0	41.0	34.0	41.0
135-139	37.44035795788843	37.4	35.0	40.8	34.0	41.0
140-144	36.827365928742616	36.2	35.0	39.4	34.0	41.0
145-149	36.15908367935235	35.0	35.0	38.0	34.0	40.6
150-154	35.567010004667566	35.0	35.0	36.4	33.6	39.0
155-159	35.117954939075524	35.0	35.0	35.6	33.0	37.0
160-164	34.81475413570281	35.0	35.0	35.0	33.0	36.2
165-169	34.631772785960614	35.0	35.0	35.0	33.0	36.0
170-174	34.521697514089894	35.0	35.0	35.0	33.0	35.6
175-178	34.23327127447662	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	89.0
30	679.0
31	4166.0
32	19494.0
33	70049.0
34	197849.0
35	495447.0
36	1350336.0
37	2816275.0
38	2754111.0
39	654724.0
40	9452.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.03773883496015	26.59140597240428	26.868557539854347	23.50229765278122
2	23.926833419426885	27.056800843219165	26.654754902107154	22.3616108352468
3	21.814163334895955	26.223674034881327	27.925471847408385	24.036690782814333
4	22.68596356600489	25.18275222673757	28.200294410089967	23.93098979716757
5	23.524596380487715	25.877930291095737	26.811455120376703	23.786018208039845
6	23.30110747162645	25.92929977575864	26.859982024860635	23.90961072775427
7	23.566088496209396	26.02538615197652	26.33359111633976	24.074934235474327
8	23.77111257752152	26.31331085943262	26.173033110684457	23.742543452361403
9	23.574496800604347	26.30770930437405	26.295992618846427	23.82180127617518
10-14	23.715646433188915	26.338600268134137	26.060815689131612	23.884937609545336
15-19	23.583748324161633	26.34386740199518	26.253368248498838	23.81901602534435
20-24	23.559163588697757	26.29106468362859	26.361954811335686	23.787816916337967
25-29	23.603338240168167	26.284032283589177	26.16409450981914	23.948534966423516
30-34	23.58871209022206	26.32771724687749	26.234239356000323	23.849331306900122
35-39	23.619087089848822	26.356009280188232	26.22150268582024	23.8034009441427
40-44	23.629485200369057	26.322619712338053	26.207325615767957	23.84056947152493
45-49	23.731562493468335	26.25612961210127	26.135164687510265	23.877143206920127
50-54	23.692580925450947	26.34329888595939	26.19295744788852	23.77116274070115
55-59	23.72399263079004	26.270519278486265	26.12398068717584	23.88150740354785
60-64	23.641502864974456	26.295182841803843	26.21674196120117	23.84657233202053
65-69	23.64989205958669	26.328730065361427	26.213574514716026	23.807803360335853
70-74	23.616844079102556	26.327208448912682	26.185153490371953	23.870793981612806
75-79	23.593327102747928	26.314710651016547	26.220716796006055	23.871245450229466
80-84	23.570932826127848	26.381071760220003	26.208066119848194	23.83992929380395
85-89	23.6126590366878	26.316865279017758	26.149673790037237	23.920801894257213
90-94	23.02301174710181	26.186226877075747	27.235759732354897	23.55500164346755
95-99	23.50212784212661	26.224167425542745	26.413328078233082	23.860376654097564
100-104	23.624549653137098	26.391485569736687	26.19399649304358	23.789968284082633
105-109	23.530454847569608	26.354290499648318	26.35117339963871	23.764081253143367
110-114	23.52548124608105	26.236286660771448	26.40983486234288	23.828397230804626
115-119	23.53845168664424	26.341715769015465	26.292465242852774	23.827367301487516
120-124	23.56337257783559	26.337022991269325	26.275065535243296	23.82453889565179
125-129	23.51772552686972	26.32637523587484	26.322367260688505	23.83353197656693
130-134	23.609158818561234	26.36712688028695	26.271630542446633	23.75208375870518
135-139	23.735888907445368	26.29312750137834	26.178511495220473	23.79247209595582
140-144	23.662549552667922	26.362354341596077	26.284302898149463	23.690793207586534
145-149	23.63609046569999	26.416674001514483	26.27081379714196	23.676421735643558
150-154	23.590605122379525	26.474369890693605	26.299715763906633	23.635309223020236
155-159	23.5404620953463	26.47590669683772	26.361298273401523	23.622332934414462
160-164	23.486981023496323	26.62569984163541	26.382786921205458	23.50453221366281
165-169	23.442568916041434	26.636771003086174	26.407496492319606	23.513163588552786
170-174	23.36998349359641	26.704440640533644	26.481707808738875	23.44386805713107
175-178	23.39342671371004	26.79052971720456	26.40970016153799	23.406343407547407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	4.0
12	5.5
13	8.5
14	22.0
15	50.0
16	91.0
17	178.5
18	367.0
19	710.5
20	1324.5
21	2437.5
22	4214.0
23	6847.5
24	10489.5
25	15468.0
26	21965.0
27	29880.5
28	39549.5
29	50571.0
30	63154.0
31	77699.5
32	93845.5
33	111533.5
34	130526.0
35	151089.0
36	173208.0
37	196610.0
38	220497.5
39	244051.0
40	266688.5
41	287172.0
42	306312.5
43	322148.0
44	332992.5
45	340454.5
46	343310.0
47	341615.0
48	337029.0
49	328620.5
50	316406.5
51	302785.5
52	288322.5
53	272153.0
54	255403.5
55	239471.5
56	223869.0
57	208004.5
58	192251.5
59	176909.5
60	161520.5
61	145633.0
62	129686.5
63	114190.0
64	99028.0
65	84572.5
66	71081.5
67	58535.0
68	47014.5
69	36834.5
70	28375.5
71	21426.5
72	15648.5
73	11003.5
74	7551.0
75	5074.5
76	3213.5
77	1962.5
78	1189.0
79	707.5
80	394.0
81	212.0
82	105.5
83	47.5
84	29.5
85	14.5
86	6.0
87	4.0
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1039.0
92-93	2232.0
94-95	3330.0
96-97	4532.0
98-99	5467.0
100-101	6551.0
102-103	7996.0
104-105	8344.0
106-107	9595.0
108-109	10686.0
110-111	12912.0
112-113	12334.0
114-115	13233.0
116-117	14166.0
118-119	15231.0
120-121	16046.0
122-123	17529.0
124-125	18593.0
126-127	18012.0
128-129	19169.0
130-131	19136.0
132-133	21366.0
134-135	95125.0
136-137	89029.0
138-139	84810.0
140-141	84447.0
142-143	86723.0
144-145	92888.0
146-147	91795.0
148-149	87066.0
150-151	94132.0
152-153	91882.0
154-155	101112.0
156-157	96541.0
158-159	88254.0
160-161	99787.0
162-163	95462.0
164-165	113628.0
166-167	98702.0
168-169	128680.0
170-171	115604.0
172-173	102466.0
174-175	118327.0
176-177	282132.0
178-179	5776584.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.54639329039523
#Duplication Level	Percentage of total
1	91.60890126922612
2	7.095029855918614
3	0.9396136159923922
4	0.20960181901898112
5	0.07164978118372442
6	0.028830928132731918
7	0.013465499591483512
8	0.010419632665496753
9	0.007336999715823953
>10	0.015150598554639546
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.971807098687098E-5	0.0	0.0	2.3887228394748393E-5	0.0	0.0
2	0.00133768479010591	2.3887228394748393E-5	1.1943614197374196E-5	3.583084259212259E-5	0.0	0.0
3	0.0013496284043032843	5.971807098687098E-5	1.1943614197374196E-5	5.971807098687098E-5	0.0	0.0
4	0.0013496284043032843	7.166168518424517E-5	2.3887228394748393E-5	5.971807098687098E-5	0.0	0.0
5	0.0013496284043032843	9.554891357899357E-5	2.3887228394748393E-5	5.971807098687098E-5	0.0	0.0
6	0.0013496284043032843	1.0749252777636777E-4	2.3887228394748393E-5	5.971807098687098E-5	0.0	0.0
7	0.0013496284043032843	1.0749252777636777E-4	2.3887228394748393E-5	5.971807098687098E-5	0.0	0.0
8	0.0013496284043032843	1.1943614197374197E-4	4.7774456789496785E-5	5.971807098687098E-5	0.0	0.0
9	0.0013496284043032843	1.1943614197374197E-4	4.7774456789496785E-5	7.166168518424517E-5	0.0	0.0
10-14	0.0013639607413401332	1.4810081604744004E-4	6.210679382634582E-5	1.289910333316413E-4	0.0	0.0
15-19	0.0013759043555375075	1.648218759237639E-4	1.2421358765269167E-4	1.4810081604744004E-4	0.0	0.0
20-24	0.001397402861092781	1.7676549012113813E-4	1.4810081604744004E-4	1.7198804444218844E-4	0.0	0.0
25-29	0.0014165126438085798	2.2692866975010975E-4	1.7198804444218844E-4	2.1020760987378587E-4	1.1943614197374196E-5	9.554891357899356E-6
30-34	0.001557447291337595	2.4603845246590845E-4	1.9109782715798717E-4	2.3887228394748393E-4	1.1943614197374196E-5	1.1943614197374196E-5
35-39	0.0019253106086167203	2.7470312653960654E-4	2.1498505555273554E-4	2.7470312653960654E-4	1.9109782715798713E-5	1.9109782715798713E-5
40-44	0.002329004768487968	2.914241864159304E-4	2.3409483826853424E-4	3.0814524629225424E-4	2.6275951234223234E-5	4.7774456789496785E-5
45-49	0.0027374763740381654	3.200888604896285E-4	2.651482351817071E-4	3.5114225740280136E-4	4.060828827107227E-5	5.0163179628971623E-5
50-54	0.003057565234527794	3.391986432054272E-4	3.0097907777382974E-4	3.893618228343988E-4	5.971807098687098E-5	6.927296234477034E-5
55-59	0.0032916600727963285	3.583084259212259E-4	3.27255029008053E-4	4.2758138826599624E-4	8.838274506056906E-5	8.360529938161937E-5
60-64	0.0034254285518069195	3.6786331727912524E-4	3.4397608888437687E-4	4.3235883394494596E-4	1.2421358765269167E-4	9.316019073951874E-5
65-69	0.003669078281433353	4.1802649690809697E-4	3.583084259212259E-4	4.490798938212697E-4	1.3854592468954066E-4	1.0749252777636777E-4
70-74	0.005644552069679045	4.634122308581188E-4	3.917505456738736E-4	4.7057839937654337E-4	1.7198804444218844E-4	1.4571209320796518E-4
75-79	0.036934432543959964	4.8491073641339225E-4	4.2758138826599624E-4	4.968543506107666E-4	2.0781888703431104E-4	1.576557074053394E-4
80-84	0.04963049443576874	5.20741579005515E-4	4.371362796238956E-4	5.255190246844647E-4	3.4397608888437687E-4	1.7676549012113813E-4
85-89	0.04976187419193985	5.398513617213138E-4	4.586347851791691E-4	5.422400845607885E-4	6.330115524608325E-4	1.8870910431851234E-4
90-94	0.050065241992553154	5.971807098687098E-4	4.89688182092342E-4	5.51794975918688E-4	6.449551666582066E-4	1.9109782715798714E-4
95-99	0.05017273452032953	6.449551666582066E-4	4.992430734502413E-4	5.661273129555368E-4	6.449551666582066E-4	1.9109782715798714E-4
100-104	0.05024678492835324	6.736198407319048E-4	5.016317962897162E-4	6.043468783871344E-4	6.449551666582066E-4	1.9109782715798714E-4
105-109	0.05032800150489538	7.022845148056027E-4	5.159641333265652E-4	6.54510058016106E-4	6.783972864108544E-4	1.9109782715798714E-4
110-114	0.05042593914131386	7.166168518424518E-4	5.446288074002633E-4	6.927296234477034E-4	6.927296234477034E-4	1.9109782715798714E-4
115-119	0.050528654223411275	7.166168518424518E-4	5.828483728318607E-4	7.357266345582505E-4	7.046732376450776E-4	1.9109782715798714E-4
120-124	0.05083202202402458	7.500589715950995E-4	6.162904925845086E-4	7.835010913477472E-4	7.357266345582505E-4	1.9109782715798714E-4
125-129	0.05125960341229058	7.954447055451214E-4	6.807860092503292E-4	8.336642709767187E-4	7.667800314714233E-4	1.9826399567641166E-4
130-134	0.05184484050796192	8.217206567793447E-4	7.237830203608763E-4	8.671063907293667E-4	8.121657654214453E-4	2.0781888703431104E-4
135-139	0.052475463337583274	8.360529938161937E-4	7.42892803076675E-4	9.22047016037288E-4	8.31275548137244E-4	2.3170611542905938E-4
140-144	0.05294365301412034	8.456078851740932E-4	7.643913086319486E-4	9.79376364184684E-4	8.479966080135679E-4	2.508158981448581E-4
145-149	0.05342378630485478	8.551627765319925E-4	8.145544882609202E-4	0.001010429761097857	8.623289450504169E-4	2.6514823518170716E-4
150-154	0.05383703535608393	8.981597876425395E-4	8.456078851740932E-4	0.0010486493265294545	8.718838364083165E-4	2.7470312653960654E-4
155-159	0.05430283630978152	9.339906302346622E-4	8.575514993714674E-4	0.0010964237833189512	8.886048962846402E-4	2.7470312653960654E-4
160-164	0.05479252449187386	9.53100412950461E-4	8.838274506056907E-4	0.0011824178055400453	9.124921246793885E-4	2.7470312653960654E-4
165-167	0.05537457662375924	9.554891357899357E-4	8.957710648030648E-4	0.0012540794907242906	9.316019073951873E-4	2.7470312653960654E-4
>>END_MODULE
