##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293921
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8369047
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.419144378087495	39.0	39.0	39.0	37.0	39.0
2	40.195974165278315	41.0	40.0	41.0	38.0	41.0
3	40.223522104727095	41.0	40.0	41.0	38.0	41.0
4	40.23293321210886	41.0	40.0	41.0	38.0	41.0
5	40.22964108099763	41.0	40.0	41.0	38.0	41.0
6	40.20398821992516	41.0	40.0	41.0	38.0	41.0
7	40.224483504513714	41.0	40.0	41.0	38.0	41.0
8	40.20987730144185	41.0	40.0	41.0	38.0	41.0
9	40.195463234941805	41.0	40.0	41.0	38.0	41.0
10-14	40.13027676866913	41.0	40.0	41.0	38.0	41.0
15-19	40.00917053040806	41.0	40.0	41.0	38.0	41.0
20-24	39.83177687973314	41.0	40.0	41.0	37.6	41.0
25-29	39.571795976292165	40.6	39.2	41.0	36.6	41.0
30-34	39.32273230153923	40.0	38.6	41.0	35.4	41.0
35-39	39.13532587402126	40.0	37.8	41.0	35.0	41.0
40-44	38.611889406284845	39.8	36.6	41.0	35.0	41.0
45-49	37.896955889959756	38.8	35.0	41.0	35.0	41.0
50-54	37.16963888480971	36.8	35.0	39.8	34.2	41.0
55-59	36.47507961181243	35.6	35.0	38.8	34.0	41.0
60-64	35.74362868317026	35.0	34.8	36.8	33.6	39.6
65-69	35.42846116170694	35.0	35.0	36.0	33.6	39.0
70-74	35.244734890364455	35.0	35.0	35.6	34.0	37.2
75-79	35.09188582642683	35.0	35.0	35.0	33.0	37.0
80-84	35.034938028188876	35.0	35.0	35.0	33.0	36.8
85-89	34.86101863210949	35.0	34.6	35.0	32.6	37.2
90-94	39.59342417471697	40.6	39.8	40.6	37.8	40.6
95-99	39.86374047383927	41.0	40.0	41.0	37.6	41.0
100-104	39.74179005513592	41.0	40.0	41.0	37.0	41.0
105-109	39.58000727505895	41.0	39.2	41.0	36.0	41.0
110-114	39.38672636586932	40.6	39.0	41.0	35.2	41.0
115-119	39.08945294440728	40.0	38.0	41.0	35.0	41.0
120-124	38.78613007022786	40.0	37.6	41.0	35.0	41.0
125-129	38.20923457641158	39.6	36.2	40.6	34.2	41.0
130-134	37.8682535477053	39.0	35.0	41.0	34.0	41.0
135-139	37.46677191705008	37.6	35.0	40.8	34.0	41.0
140-144	36.85917805573308	36.4	35.0	39.4	34.0	41.0
145-149	36.17841469789103	35.2	35.0	38.0	34.0	40.6
150-154	35.57239088917099	35.0	35.0	36.4	33.4	39.0
155-159	35.11400306895932	35.0	35.0	35.6	33.0	37.0
160-164	34.81170514692673	35.0	35.0	35.0	33.0	36.2
165-169	34.628987932487476	35.0	35.0	35.0	33.0	36.0
170-174	34.52279443870848	35.0	35.0	35.0	33.0	35.6
175-178	34.22815778183106	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	6.0
29	63.0
30	644.0
31	3940.0
32	19249.0
33	69396.0
34	197533.0
35	501990.0
36	1362677.0
37	2821835.0
38	2745683.0
39	638371.0
40	7660.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.03116471923267	26.611142224437263	26.905751634564844	23.451941421765227
2	23.84953746824459	27.10386260227718	26.684710935426697	22.361888994051533
3	21.77999478315751	26.263743052225657	27.949179876752993	24.00708228786384
4	22.631453736608243	25.201471565400457	28.270817453886927	23.896257244104376
5	23.484358493864356	25.880509453465848	26.847166708467523	23.787965344202274
6	23.306990628682094	25.953480724866285	26.88497268565943	23.85455596079219
7	23.55792720485379	25.99641273373181	26.367601950377384	24.078058111037016
8	23.722485965247895	26.344743911702253	26.198622137024678	23.73414798602517
9	23.572134318280206	26.341625277047676	26.334217026144074	23.752023378528044
10-14	23.70320778459005	26.357794382084364	26.076780307244064	23.862217526081526
15-19	23.562546607755937	26.372644340508543	26.26625946777453	23.79854958396099
20-24	23.52365807002876	26.298767350691183	26.417263518773403	23.760311060506652
25-29	23.575355712544095	26.30570959871536	26.19668882251468	23.922245866225868
30-34	23.561368456886427	26.346163428165713	26.258184474289603	23.83428364065825
35-39	23.601786439961444	26.365028180627974	26.237197616407222	23.795987763003364
40-44	23.604092556774983	26.36528388477207	26.223098042106823	23.807525516346125
45-49	23.71872448559555	26.269275342819796	26.167060598417002	23.84493957316765
50-54	23.65884431046928	26.373703003460253	26.207600459168173	23.759852226902296
55-59	23.70680437091583	26.29778754976522	26.13632591620049	23.859082163118455
60-64	23.612444762229202	26.34017708348394	26.220134741745387	23.827243412541478
65-69	23.616342458107834	26.345542090993156	26.234960802586006	23.803154648313004
70-74	23.58756020846818	26.3376917347937	26.210279378285247	23.864468678452873
75-79	23.55745642245766	26.347377425410563	26.248094914510578	23.8470712376212
80-84	23.571190363729585	26.385379362787663	26.22374805637966	23.819682217103093
85-89	23.586692726184953	26.345279217573996	26.159707311955593	23.90832074428546
90-94	22.976262369210275	26.220925983051085	27.284849800747924	23.517961846990715
95-99	23.4828202442987	26.23652066463778	26.442754579416412	23.837904511647103
100-104	23.599891897657525	26.411056161084474	26.217435517611875	23.77161642364613
105-109	23.50415221135951	26.391897419904147	26.36819603894486	23.735754329791487
110-114	23.491080223500237	26.259028415483304	26.44430778981534	23.80558357120111
115-119	23.500101463553673	26.374855356346195	26.31525520020076	23.809787979899372
120-124	23.52667916207813	26.363990849663672	26.29975616930553	23.80957381895267
125-129	23.477191731079945	26.364735487424955	26.34447738869296	23.81359539280214
130-134	23.580965809209463	26.389649641334056	26.283916858235422	23.745467691221062
135-139	23.697779142403675	26.325122281567122	26.195505111876184	23.781593464153023
140-144	23.622675218841582	26.400513975612185	26.30375062031095	23.673060185235283
145-149	23.617752644017685	26.440552856574982	26.295974068602796	23.64572043080454
150-154	23.549058600759636	26.520775905270117	26.31705637299132	23.613109120978926
155-159	23.512998643201218	26.514686115191893	26.371822937127597	23.60049230447929
160-164	23.44872557219116	26.650416238743297	26.433598516067967	23.46725967299757
165-169	23.405521662249505	26.684580710963495	26.420902025335536	23.488995601451464
170-174	23.33187393767437	26.75057637531053	26.51664216023456	23.400907526780543
175-178	23.353654082538657	26.84183935053619	26.43333191355127	23.37117465337388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	2.0
12	5.0
13	11.0
14	20.0
15	45.0
16	88.0
17	174.0
18	358.0
19	715.5
20	1380.5
21	2509.5
22	4316.0
23	7006.0
24	10736.0
25	15691.0
26	22361.0
27	30431.5
28	39976.5
29	51449.5
30	64396.5
31	78642.5
32	94418.5
33	112186.0
34	131732.0
35	152267.5
36	174026.5
37	197415.5
38	220894.0
39	244367.0
40	267499.5
41	288514.0
42	307045.0
43	322211.5
44	333220.0
45	340378.0
46	343395.0
47	341557.5
48	336340.5
49	327456.0
50	315038.0
51	301919.5
52	287480.5
53	271219.5
54	254882.5
55	238991.5
56	223411.0
57	207627.5
58	191675.5
59	175797.0
60	159745.0
61	144385.0
62	128979.0
63	113469.0
64	98481.0
65	83938.0
66	70409.0
67	57943.5
68	46618.0
69	36797.5
70	28282.0
71	21047.5
72	15386.5
73	10870.5
74	7417.5
75	4936.5
76	3216.5
77	1991.5
78	1179.0
79	680.5
80	369.5
81	209.0
82	114.0
83	56.0
84	22.0
85	10.0
86	4.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1186.0
92-93	2313.0
94-95	3485.0
96-97	4663.0
98-99	6429.0
100-101	6729.0
102-103	7869.0
104-105	8761.0
106-107	9616.0
108-109	10552.0
110-111	11792.0
112-113	12829.0
114-115	12885.0
116-117	13552.0
118-119	14124.0
120-121	14988.0
122-123	15919.0
124-125	16362.0
126-127	17037.0
128-129	18290.0
130-131	18853.0
132-133	20952.0
134-135	100574.0
136-137	92707.0
138-139	82435.0
140-141	81897.0
142-143	80265.0
144-145	88651.0
146-147	83102.0
148-149	89572.0
150-151	85790.0
152-153	82915.0
154-155	86698.0
156-157	81156.0
158-159	84873.0
160-161	88193.0
162-163	90786.0
164-165	95455.0
166-167	95328.0
168-169	93152.0
170-171	98210.0
172-173	104044.0
174-175	104077.0
176-177	284113.0
178-179	5945868.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.44066840073607
#Duplication Level	Percentage of total
1	91.39096206957981
2	7.325192513457461
3	0.9396063337449548
4	0.1994258247921403
5	0.0716495105428969
6	0.02883087615292925
7	0.014501309621123937
8	0.007293758339711824
9	0.00524073542091325
>10	0.017297068348046757
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.169275067997587E-5	1.1948791779995978E-5	0.0	2.3897583559991957E-5	0.0	0.0
2	0.0012546231368995777	1.1948791779995978E-5	1.1948791779995978E-5	3.5846375339987933E-5	0.0	0.0
3	0.0012665719286795738	3.5846375339987933E-5	1.1948791779995978E-5	3.5846375339987933E-5	0.0	0.0
4	0.0012665719286795738	4.7795167119983914E-5	2.3897583559991957E-5	3.5846375339987933E-5	0.0	0.0
5	0.0012785207204595696	7.169275067997587E-5	3.5846375339987933E-5	3.5846375339987933E-5	0.0	0.0
6	0.0012785207204595696	8.364154245997185E-5	4.7795167119983914E-5	3.5846375339987933E-5	0.0	0.0
7	0.0012785207204595696	8.364154245997185E-5	4.7795167119983914E-5	3.5846375339987933E-5	0.0	0.0
8	0.0012785207204595696	9.559033423996783E-5	5.974395889997989E-5	4.7795167119983914E-5	0.0	0.0
9	0.0012785207204595696	1.0753912601996381E-4	5.974395889997989E-5	4.7795167119983914E-5	0.0	0.0
10-14	0.0012785207204595696	1.4816501807195012E-4	7.169275067997587E-5	8.364154245997185E-5	0.0	0.0
15-19	0.0012976387873075632	1.792318766999397E-4	7.169275067997587E-5	1.1709815944396058E-4	2.389758355999196E-6	0.0
20-24	0.001345433954427547	1.9596018519193401E-4	9.798009259596703E-5	1.2426743451195816E-4	1.1948791779995978E-5	0.0
25-29	0.0013812803297675352	2.0312946025993162E-4	1.5055477642794934E-4	1.5055477642794934E-4	1.1948791779995978E-5	0.0
30-34	0.0015294453478394852	2.1746801039592683E-4	1.9834994354793323E-4	1.7206260163194205E-4	1.4338550135995173E-5	0.0
35-39	0.0017301850497434177	2.3658607724392038E-4	2.5331438573591476E-4	1.9834994354793323E-4	2.8677100271990347E-5	1.1948791779995977E-5
40-44	0.0019763301604113345	2.3897583559991955E-4	2.915505194319019E-4	2.3897583559991955E-4	6.930299232397667E-5	2.8677100271990347E-5
45-49	0.002210526479299256	2.557041440919139E-4	3.05889069567897E-4	2.7482221093990745E-4	8.364154245997185E-5	5.01849254759831E-5
50-54	0.002380199322575199	2.700426942279091E-4	3.1066858627989545E-4	2.9871979449989943E-4	9.081081752796943E-5	6.452347561197827E-5
55-59	0.0025785492661231325	2.9871979449989943E-4	3.29786653127889E-4	3.321764114838882E-4	1.1470840108796139E-4	8.125178410397265E-5
60-64	0.002781678726383063	3.6802278682387615E-4	3.608535117558785E-4	3.8714085367186964E-4	1.3621622629195415E-4	1.2187767615595897E-4
65-69	0.002944182294591009	3.9669988709586647E-4	3.871408536718697E-4	4.014794038078649E-4	1.5772405149594692E-4	1.4338550135995173E-4
70-74	0.0047054342029624165	4.325462624358544E-4	4.086486788758625E-4	4.683926377758423E-4	1.8879091012393646E-4	1.4816501807195012E-4
75-79	0.03620244933503182	4.7317215448784076E-4	4.3015650407985523E-4	5.233570799638239E-4	1.9118066847993565E-4	1.648933265639445E-4
80-84	0.04935806908480738	4.803414295558383E-4	4.468848125718496E-4	5.592034553038118E-4	2.652631775159107E-4	1.9596018519193401E-4
85-89	0.049451269660691355	5.01849254759831E-4	4.8034142955583823E-4	5.711522470838077E-4	4.492745709278488E-4	2.0312946025993162E-4
90-94	0.04970219428807127	5.257468383198231E-4	5.424751468118174E-4	5.783215221518053E-4	4.540540876398472E-4	2.0312946025993162E-4
95-99	0.04980495389737923	5.520341802358143E-4	5.831010388638038E-4	5.902703139318013E-4	4.540540876398472E-4	2.0312946025993162E-4
100-104	0.049840800272719234	5.735420054398069E-4	6.093883807797948E-4	6.332859643397869E-4	4.5644384599584635E-4	2.0312946025993162E-4
105-109	0.04987425688970322	6.261166892717892E-4	6.356757226957861E-4	6.619630646117772E-4	4.7556191284383996E-4	2.0312946025993162E-4
110-114	0.04995072915709518	6.47624514475782E-4	6.858606481717691E-4	6.834708898157699E-4	4.899004629798351E-4	2.0312946025993162E-4
115-119	0.05000808335763916	6.691323396797748E-4	7.09758231731761E-4	7.001991983077644E-4	5.01849254759831E-4	2.0312946025993162E-4
120-124	0.050340259769123055	7.097582317317612E-4	7.360455736477522E-4	7.288762985797546E-4	5.01849254759831E-4	2.0790897697193E-4
125-129	0.050875565640866885	7.647226739197426E-4	7.814509824117369E-4	7.933997741917329E-4	5.042390131158302E-4	2.3897583559991955E-4
130-134	0.05145866667973068	7.886202574797345E-4	8.005690492597306E-4	8.340256662437192E-4	5.137980465398271E-4	2.7960172765190583E-4
135-139	0.05196529545120251	8.340256662437192E-4	8.053485659717289E-4	8.364154245997185E-4	5.137980465398271E-4	2.915505194319019E-4
140-144	0.05238589292185837	8.79431075007704E-4	8.340256662437192E-4	8.603130081597105E-4	5.353058717438198E-4	2.9871979449989943E-4
145-149	0.05273957715854625	9.057184169236952E-4	8.627027665157097E-4	8.866003500757017E-4	5.496444218798151E-4	2.9871979449989943E-4
150-154	0.052916419276890184	9.750214092476717E-4	9.057184169236952E-4	9.224467254156895E-4	5.61593213659811E-4	2.9871979449989943E-4
155-159	0.05319841076289809	0.0010275960930796542	9.29616000483687E-4	9.582931007556774E-4	5.663727303718094E-4	2.9871979449989943E-4
160-164	0.05361422871684196	0.0010777810185556374	9.559033423996782E-4	9.91749717739666E-4	5.854907972198029E-4	2.9871979449989943E-4
165-167	0.054143958485755116	0.001087340051979634	9.9971557892633E-4	0.001003698509519662	5.854907972198029E-4	3.146515168732274E-4
>>END_MODULE
