##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293934
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7314317
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-170
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.15629771036721	41.0	40.0	41.0	38.0	41.0
2	40.24728337587775	41.0	40.0	41.0	39.0	41.0
3	40.30557754059607	41.0	40.0	41.0	39.0	41.0
4	40.29055207752139	41.0	40.0	41.0	39.0	41.0
5	40.30433422560165	41.0	40.0	41.0	39.0	41.0
6	40.27228516346776	41.0	40.0	41.0	39.0	41.0
7	40.264634141506306	41.0	40.0	41.0	39.0	41.0
8	40.252714641708856	41.0	40.0	41.0	39.0	41.0
9	40.221843680004575	41.0	40.0	41.0	38.0	41.0
10-14	40.17482911938326	41.0	40.0	41.0	38.0	41.0
15-19	40.052938695438	41.0	40.0	41.0	38.0	41.0
20-24	39.85315968668025	41.0	40.0	41.0	37.6	41.0
25-29	39.53230449268195	40.6	39.0	41.0	36.2	41.0
30-34	39.082204230415506	40.0	37.8	41.0	35.0	41.0
35-39	38.54135159851562	39.8	36.6	41.0	35.0	41.0
40-44	37.976142871576386	39.0	35.2	41.0	34.8	41.0
45-49	37.43346581779269	37.2	35.0	40.4	35.0	41.0
50-54	36.74308592312857	35.8	35.0	39.2	34.2	41.0
55-59	36.114726310057385	35.0	35.0	37.4	34.0	40.2
60-64	35.39082284784759	35.0	34.8	36.0	33.4	39.0
65-69	35.31531802627641	35.0	35.0	35.8	34.0	37.2
70-74	35.11003315825661	35.0	35.0	35.0	34.0	36.6
75-79	35.01156482553326	35.0	35.0	35.0	34.0	36.0
80-84	34.9712040098891	35.0	35.0	35.0	33.2	36.0
85-89	38.92354386609166	39.8	38.8	39.8	37.0	40.0
90-94	39.99360629320244	41.0	40.0	41.0	38.0	41.0
95-99	39.873111016301564	41.0	40.0	41.0	37.6	41.0
100-104	39.71106894990151	41.0	39.8	41.0	36.8	41.0
105-109	39.48421291730604	40.6	39.0	41.0	35.6	41.0
110-114	39.15214434665262	40.0	38.2	41.0	35.0	41.0
115-119	38.70838034063038	40.0	37.4	41.0	35.0	41.0
120-124	37.7311035119479	38.8	35.2	40.2	33.8	40.6
125-129	37.8939777877673	38.8	35.0	41.0	34.8	41.0
130-134	37.314646132715964	37.2	35.0	40.4	34.2	41.0
135-139	36.63796983254876	35.8	35.0	39.0	34.0	41.0
140-144	36.00247605371034	35.0	35.0	37.2	34.0	40.0
145-149	35.46956330555165	35.0	35.0	36.2	34.0	38.4
150-154	35.07722064236758	35.0	35.0	35.4	34.0	36.8
155-159	34.833320411630055	35.0	35.0	35.0	33.2	36.0
160-164	34.69419556415101	35.0	35.0	35.0	33.0	36.0
165-169	34.61493268601379	35.0	35.0	35.0	33.0	35.4
170	34.131108854268696	35.0	34.0	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	43.0
30	442.0
31	3028.0
32	13938.0
33	50522.0
34	147895.0
35	427491.0
36	1300968.0
37	2493139.0
38	2263794.0
39	605079.0
40	7974.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.492637795162555	25.49177182230412	27.32885380822297	23.686736574310356
2	23.61032479177482	25.667413649148653	26.835164513651787	23.887097045424746
3	23.595135403620052	26.230733505260982	26.443015800381637	23.731115290737332
4	23.61603961108057	26.3690239293703	26.27750478957912	23.737431669970004
5	24.065172455610004	26.27917275119468	25.934984770279986	23.720670022915332
6	23.653062890219278	26.646917271974953	26.01611332951525	23.68390650829052
7	23.652994531136674	26.620311917025198	26.1713978215601	23.55529573027803
8	24.18877934877583	26.30721364687913	25.87823305990156	23.62577394444348
9	23.758458923779212	26.4788359596665	25.92788909750562	23.83481601904867
10-14	23.772773315676638	26.445848600764776	26.11845781362771	23.662920269930876
15-19	23.61315485779465	26.327303014075	26.353304074734524	23.706238053395825
20-24	23.78758809605873	26.2141632636376	26.151841655208546	23.846406985095122
25-29	23.775236977013712	26.320962025572587	26.091270039294166	23.812530958119535
30-34	23.656024205677713	26.306365994254826	26.155500233309546	23.882109566757908
35-39	23.733398484096327	26.287255529121857	26.13403274700837	23.845313239773446
40-44	23.836276169053107	26.221800340346203	26.012572329036328	23.929351161564366
45-49	23.87462834875765	26.242649860540634	26.011505927347695	23.871215863354024
50-54	23.965329913920876	26.167432994769023	25.97101547553927	23.896221615770823
55-59	23.893736079527315	26.221163233696327	25.996472397901265	23.88862828887509
60-64	23.83475859741928	26.212134366065897	26.047749912944706	23.905357123570116
65-69	23.905346186116898	26.194453972941012	25.996133336851546	23.90406650409054
70-74	23.83860311222497	26.215262477685886	26.022429708747925	23.92370470134122
75-79	23.79004355430589	26.24692367038508	26.007650475088788	23.95538230022024
80-84	23.84668862451545	26.204300415199395	25.999950508024195	23.949060452260955
85-89	23.4586523936548	26.06658147302065	26.63752199966176	23.83724413366279
90-94	23.669411050043994	26.487964807750142	26.107774268929663	23.7348498732762
95-99	23.60221221831168	26.464448191730693	26.223728676288914	23.70961091366871
100-104	23.473128604999456	26.32599584416569	26.433274459653067	23.767601091181785
105-109	23.636024527259263	26.218555822755196	26.248901075026236	23.896518574959302
110-114	23.604921998004343	26.3217037266788	26.187741014217647	23.885633261099212
115-119	23.540790913487022	26.282427167022703	26.222599050408395	23.954182869081876
120-124	23.632731803879267	26.272012827010354	26.210232951703222	23.885022417407157
125-129	23.760976148978912	26.184083195296008	26.106109076220708	23.948831579504372
130-134	23.79720975923244	26.22867663551486	26.0993875103103	23.8747260949424
135-139	23.836162479438553	26.20964479408117	26.11165041992872	23.842542306551554
140-144	23.751811208046195	26.298357304137742	26.136673657038994	23.813157830777072
145-149	23.660056725057466	26.354620290946045	26.195233733059165	23.790089250937328
150-154	23.685967525845225	26.394605515843217	26.188119367487438	23.731307590824123
155-159	23.607191279696345	26.423610430737238	26.273558757438902	23.69563953212752
160-164	23.485005734022167	26.53102837204486	26.304071871213434	23.67989402271953
165-169	23.49874087065661	26.530790879959305	26.357098282581454	23.61336996680263
170	23.344640457508927	26.69850876559885	26.25153367907701	23.705317097815215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	2.5
14	8.0
15	16.0
16	29.0
17	60.0
18	134.5
19	304.0
20	666.0
21	1329.5
22	2567.0
23	4578.5
24	7616.5
25	12285.0
26	18892.0
27	27432.5
28	37953.0
29	50404.0
30	64633.5
31	79901.5
32	95503.5
33	111263.5
34	127110.0
35	143616.5
36	159437.5
37	174552.5
38	190352.0
39	205898.0
40	220854.0
41	235145.0
42	248538.5
43	260203.0
44	269399.0
45	276211.5
46	280641.5
47	282017.5
48	280020.5
49	274961.0
50	267217.0
51	256710.0
52	244695.0
53	232410.5
54	219677.5
55	206861.0
56	194434.0
57	182369.5
58	170436.0
59	158786.5
60	147062.0
61	135320.0
62	123615.5
63	111316.0
64	98714.5
65	86348.0
66	74190.0
67	62012.5
68	50641.0
69	40402.5
70	31193.5
71	23449.0
72	17096.5
73	12199.5
74	8543.0
75	5669.5
76	3543.0
77	2221.5
78	1305.0
79	755.0
80	447.0
81	234.5
82	129.5
83	68.5
84	27.5
85	15.0
86	10.0
87	4.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	448.0
92-93	1149.0
94-95	1840.0
96-97	2447.0
98-99	3030.0
100-101	3543.0
102-103	4016.0
104-105	4466.0
106-107	5093.0
108-109	5506.0
110-111	5912.0
112-113	6246.0
114-115	6740.0
116-117	6861.0
118-119	8944.0
120-121	7729.0
122-123	8216.0
124-125	8474.0
126-127	9077.0
128-129	9540.0
130-131	63375.0
132-133	71385.0
134-135	71296.0
136-137	66987.0
138-139	63865.0
140-141	63063.0
142-143	62557.0
144-145	60806.0
146-147	63364.0
148-149	69677.0
150-151	62400.0
152-153	61814.0
154-155	64838.0
156-157	63322.0
158-159	63930.0
160-161	67332.0
162-163	70856.0
164-165	70513.0
166-167	73283.0
168-169	131037.0
170-171	5819340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.21075804102455
#Duplication Level	Percentage of total
1	89.31085510331037
2	8.562138278033494
3	1.4422402937456482
4	0.3273428059207729
5	0.14374377862751142
6	0.07443032093293898
7	0.03330556985313645
8	0.026196876741980256
9	0.018989684707655483
>10	0.06075728812648006
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.3671816520941054E-5	0.0	0.0
2	0.0	0.0	1.3671816520941054E-5	1.3671816520941054E-5	0.0	0.0
3	0.0	1.3671816520941054E-5	1.3671816520941054E-5	1.3671816520941054E-5	1.3671816520941054E-5	0.0
4	0.0	1.3671816520941054E-5	1.3671816520941054E-5	1.3671816520941054E-5	1.3671816520941054E-5	0.0
5	0.0	1.3671816520941054E-5	2.7343633041882107E-5	2.7343633041882107E-5	1.3671816520941054E-5	0.0
6	1.3671816520941054E-5	1.3671816520941054E-5	4.101544956282316E-5	2.7343633041882107E-5	2.7343633041882107E-5	0.0
7	2.7343633041882107E-5	1.3671816520941054E-5	6.835908260470526E-5	2.7343633041882107E-5	2.7343633041882107E-5	0.0
8	2.7343633041882107E-5	1.3671816520941054E-5	6.835908260470526E-5	2.7343633041882107E-5	2.7343633041882107E-5	0.0
9	4.101544956282316E-5	1.3671816520941054E-5	9.570271564658737E-5	4.101544956282316E-5	2.7343633041882107E-5	0.0
10-14	6.562471930051704E-5	1.9140543129317474E-5	1.6679616155548083E-4	5.1952902779575996E-5	5.468726608376421E-5	0.0
15-19	8.749962573402274E-5	2.7343633041882104E-5	2.269521542476215E-4	7.109344590889347E-5	8.476526242983454E-5	0.0
20-24	1.2031198538428127E-4	3.2812359650258526E-5	2.6523324050625643E-4	8.749962573402274E-5	1.3124943860103408E-4	0.0
25-29	1.285150752968459E-4	5.7421629387952425E-5	3.363266864151499E-4	1.0937453216752844E-4	1.4765561842616337E-4	0.0
30-34	2.132803377266804E-4	7.382780921308168E-5	4.101544956282316E-4	1.2304634868846948E-4	1.531243450345398E-4	0.0
35-39	4.3749812867011366E-4	8.749962573402274E-5	4.867166681455015E-4	1.2304634868846948E-4	1.7499925146804548E-4	1.3671816520941052E-5
40-44	6.726533728302998E-4	9.570271564658737E-5	5.632788406627713E-4	1.2304634868846948E-4	2.3515524416018612E-4	1.9140543129317474E-5
45-49	8.093715380397104E-4	9.570271564658737E-5	5.988255636172182E-4	1.585930716429162E-4	2.5976451389788E-4	5.7421629387952425E-5
50-54	9.406209766407445E-4	1.0664016886334023E-4	6.289035599632884E-4	1.8593670468479832E-4	2.6249887720206815E-4	7.92965358214581E-5
55-59	0.0010199175124622026	1.1757762208009306E-4	6.535128297009824E-4	2.1874906433505683E-4	3.089830533732678E-4	1.2031198538428127E-4
60-64	0.0010855422317627198	1.2304634868846948E-4	7.082000957847465E-4	2.2968651755180965E-4	3.554672295444674E-4	1.503899817303516E-4
65-69	0.0011484325877590485	1.3945252851359875E-4	7.628873618685108E-4	2.488270606811272E-4	3.828108625863495E-4	1.7226488816385728E-4
70-74	0.010595657803729315	1.613274349471044E-4	8.367151710815925E-4	2.6796760381044465E-4	4.2382631214917264E-4	2.3515524416018612E-4
75-79	0.018615545374913337	1.6953052485966906E-4	8.476526242983453E-4	2.843737836355739E-4	4.839823048413133E-4	2.5976451389788E-4
80-84	0.01873312299699343	2.4062397076856254E-4	8.667931674276628E-4	3.2538923319839705E-4	6.781220994386763E-4	2.5976451389788E-4
85-89	0.0188096851695107	2.7343633041882105E-4	9.187460702072388E-4	3.609359561528438E-4	6.835908260470527E-4	2.5976451389788E-4
90-94	0.018850700619073523	2.7343633041882105E-4	9.433553399449327E-4	4.4570121858267833E-4	7.246062756098758E-4	2.5976451389788E-4
95-99	0.01889718479524472	2.8984251024395034E-4	9.624958830742502E-4	5.085915745790072E-4	7.492155453475697E-4	2.5976451389788E-4
100-104	0.018965543877849426	3.1171741667745603E-4	0.0010089800592454497	5.304664810125128E-4	7.65621725172699E-4	2.5976451389788E-4
105-109	0.01902296550723738	3.3632668641514994E-4	0.0010199175124622026	5.359352076208893E-4	7.710904517810753E-4	2.5976451389788E-4
110-114	0.01905304350358345	3.5546722954446735E-4	0.0010335893289831436	5.605444773585832E-4	7.7929354169364E-4	2.5976451389788E-4
115-119	0.019170621125663544	3.5546722954446735E-4	0.0010910109583710961	5.742162938795242E-4	8.203089912564631E-4	2.5976451389788E-4
120-124	0.019419448186344675	3.5546722954446735E-4	0.0011484325877590485	5.906224737046535E-4	8.285120811690278E-4	2.5976451389788E-4
125-129	0.0196217910708546	3.582015928486556E-4	0.0012031198538428127	6.261691966591001E-4	8.394495343857807E-4	2.5976451389788E-4
130-134	0.019949914667357186	3.691390460654084E-4	0.0012578071199265768	6.644502829177352E-4	8.886680738611685E-4	2.5976451389788E-4
135-139	0.020144054461954546	3.691390460654084E-4	0.0012687445731433297	6.999970058721819E-4	9.433553399449327E-4	2.5976451389788E-4
140-144	0.02026436644733883	3.691390460654084E-4	0.001312494386010341	7.109344590889347E-4	9.706989729868148E-4	2.5976451389788E-4
145-149	0.02044483442541525	3.828108625863495E-4	0.0013808534686150463	7.246062756098758E-4	9.816364262035678E-4	2.6796760381044465E-4
150-154	0.020652646036533554	4.074201323240434E-4	0.0014464781879155633	7.300750022182523E-4	9.925738794203205E-4	2.7890505702719744E-4
155-159	0.020846785831130917	4.2382631214917264E-4	0.0015011654539993278	7.382780921308168E-4	0.0010035113326370732	2.871081469397621E-4
>>END_MODULE
