FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517270

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4944985
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length186-302
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT464940.9402252989645066TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)