FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517274

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701892
Total Bases211.6 Mbp
Sequences flagged as poor quality0
Sequence length233-302
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT111721.5916978680480758TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)