FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517283

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286870
Total Bases991.5 Mbp
Sequences flagged as poor quality0
Sequence length186-302
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2039116.203804835603477TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA43200.13143203108124143TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG41860.12735520419122145TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)