FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517285

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643400
Total Bases194.1 Mbp
Sequences flagged as poor quality0
Sequence length202-302
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT30451747.32934410941871TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTAT20650.3209511967671744TruSeq Adapter, Index 1 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT18030.2802300279763755TruSeq Adapter, Index 13 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT12490.19412496114392291TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC11950.18573204849238423TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGCTCTCGTAT7930.12325147653092944TruSeq Adapter, Index 1 (97% over 36bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA7490.116412806963009TruSeq Adapter, Index 1 (97% over 34bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT7330.11392601802921977TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)