FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517291

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894666
Total Bases571.2 Mbp
Sequences flagged as poor quality0
Sequence length190-302
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT777764.10499792575578TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT35820.18905706863373278TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)