FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517296

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931148
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length186-302
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT454970.7670858997280122TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG246850.41619261566226307TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT232670.392284933709292TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT153730.259190969437957TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)