FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517306

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341830
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length186-302
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT102487230.66798730037135TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT127640.38194641857904205TruSeq Adapter, Index 27 (97% over 39bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT66270.19830452177399807TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT57220.17122355116807259TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATACTCGATCTCGTAT54830.16407178103015416TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC51740.1548253501823851TruSeq Adapter, Index 27 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT44630.1335495821151884TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)