FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517311

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054788
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length186-302
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT123890630.554149810051722TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC84500.20839560539293298TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT76160.18782732907367783TruSeq Adapter, Index 2 (97% over 37bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT72550.17892427421606258TruSeq Adapter, Index 2 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT41080.10131232508333357TruSeq Adapter, Index 2 (97% over 35bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA40680.10032583701046763TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)