FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517313

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419425
Total Bases428.2 Mbp
Sequences flagged as poor quality0
Sequence length193-302
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT682494.808214593937686TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT121030.852669214646776TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTTT27480.19359952093277208TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGGAT27150.19127463585606846TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGTAT22970.1618260915511563TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGGAT17230.12138718142909982TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)