FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517325

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5058490
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length186-302
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT151192529.88886011438196TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT164990.32616452735895496TruSeq Adapter, Index 7 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT102560.2027482509602668TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT100090.19786537089131342TruSeq Adapter, Index 7 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT71820.14197912815879837TruSeq Adapter, Index 9 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC67880.13419024254273507TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)