FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517332

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736753
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length252-302
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT70077810.402311024316907TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT150910.224009994132188TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT67530.10024116959609475TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)