FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517340

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244512
Total Bases375.4 Mbp
Sequences flagged as poor quality0
Sequence length246-302
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT115830.9307262605744259TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT27400.22016661952636857TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)