FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517351

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380
Total Bases416.3 kbp
Sequences flagged as poor quality0
Sequence length296-302
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT30.21739130434782608TruSeq Adapter, Index 7 (97% over 36bp)
TCTTTGGTTGACAGCACCTAGCTTAGTATACAAACTAGGAGCTTTGCAAA20.14492753623188406No Hit
GTTTGTCAACAACACTGGCAGAGTGGCCATAGTATAGCAGACATTGAAAG20.14492753623188406No Hit
CTGAGAATGATTCTGTCGGGTTTTTATACGAAGATATTTCCTTTTCTGCC20.14492753623188406No Hit
CACCAGTAAGGGAGCAGGGAAGGAAGAGACAAGATCGGAAGAGCACACGT20.14492753623188406No Hit
GTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGAT20.14492753623188406No Hit
AGTGAGAATCTGCAAAGAATATTAATTCTCTTCTGGAATACAAGAAAGAT20.14492753623188406No Hit
TTTCCAGGAATTTATCCATCTCCTCTAGGTTTTCTAGTTTATGCATGTAA20.14492753623188406No Hit
ATTATATTTAGATGCAACAATTCTAATTGTACCTTAATTTAAAAGTTCTG20.14492753623188406No Hit
CCTTTCTTTTCATAGAGCAGTTGGGAAACACTCTGTTTGTAAAGTCTGCA20.14492753623188406No Hit
GAGTTGAACGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGG20.14492753623188406TruSeq Adapter, Index 7 (97% over 36bp)
GAGAAGGACAAGATTTGATTACTAAACCATTATGCACATTTGCAGAATTT20.14492753623188406No Hit
TGTAGTCTGTGCAATCTTAGGCAACTCACTTCATCTCCCTGTGCCTCAGT20.14492753623188406No Hit
CAGTTTCCAGTTGGAGATTTCAATCGCTTTGAGACCAAATGTAGAAAAGG20.14492753623188406No Hit
CATTTTCCATCTCATCACAAGTAAACAAAGTGCCACCTGCTTTGGAAATT20.14492753623188406No Hit
GTTTTCACTCAAAACTGCAAACACACAGCAACAGTAGCTTAATCTTACAG20.14492753623188406No Hit
TCCACACATCCTCAAGTCCACCTGTTAGCCCTCTGGAACCTGCGGTTAGA20.14492753623188406No Hit
CTTTGGGAGGCTGAGGGAGGACAATCGCTTGGACCCGTGAGGCAGAAGTT20.14492753623188406No Hit
GCCCCGAGCTTTTATCCCCCGACTAAGAGATCGGAAGAGCACACGTCTGA20.14492753623188406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGAGAAGTTTCTATAATACTCTAAATTTTATCTTGAGGCTCATATGT20.14492753623188406No Hit
CATCTCCAGTATGTCATGTGGAGGGATAATGATTGTCTTAGTCCATAGAT20.14492753623188406No Hit
AGGAGGGAACACTTGAGCATAAACTAACGATATTAAGGAAGAAAGTAACA20.14492753623188406No Hit
ACAACTTACTGCACAGTTGTCATTTCAAGAGGTAAAGCATATCCAGAATC20.14492753623188406No Hit
GCTTTGGAGGATACCATGGGGCATTTGGGACAGATCGGAAGAGCACACGT20.14492753623188406No Hit
CAAATACAAAAATTAGCTGGGTGTGGTGGTGCATAGATCGGAAGAGCACA20.14492753623188406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)