FastQCFastQC Report
Wed 13 Sep 2023
stdin:ERR3517355

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3517355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263893
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length288-302
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT158128430.040200285226167TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT156550.29740346165851017TruSeq Adapter, Index 7 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT87870.16692968493090568TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTAT74250.1410552988064157TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC63410.12046217504801104TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)