##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478968
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2554104
Total Bases	442.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-184
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.471214562915215	40.0	40.0	41.0	37.0	41.0
2	39.61206865499604	40.0	40.0	41.0	37.0	41.0
3	39.69110106714527	40.0	40.0	41.0	37.0	41.0
4	39.697199879096544	40.0	40.0	41.0	37.0	41.0
5	39.67132544328657	40.0	40.0	41.0	37.0	41.0
6	39.670723666694855	40.0	40.0	41.0	37.0	41.0
7	39.66453402054106	40.0	40.0	41.0	37.0	41.0
8	39.65696698333349	40.0	40.0	41.0	37.0	41.0
9	39.63731234123591	40.0	40.0	41.0	37.0	41.0
10-14	39.56075422144126	40.0	39.8	41.0	37.0	41.0
15-19	39.39512580537049	40.0	39.0	41.0	36.4	41.0
20-24	39.170069817047384	40.0	38.4	41.0	36.0	41.0
25-29	38.88813337279923	40.0	38.0	41.0	35.0	41.0
30-34	38.742946410952726	40.0	38.0	41.0	35.0	41.0
35-39	38.58227127791194	40.0	37.0	41.0	34.6	41.0
40-44	38.138042147069974	39.6	36.0	40.4	34.0	41.0
45-49	37.45144254110248	38.2	35.0	40.0	33.4	41.0
50-54	36.75957596088491	36.6	35.0	39.6	33.0	41.0
55-59	36.08633861424594	35.6	34.4	38.8	33.0	40.0
60-64	35.29316887644356	35.0	33.8	37.0	32.4	39.0
65-69	35.21843182579879	35.0	34.0	36.2	33.0	39.0
70-74	34.981159028763116	35.0	34.0	36.0	33.0	38.6
75-79	34.855587399730005	35.0	34.0	35.6	32.0	38.0
80-84	34.79764582804772	35.0	34.0	35.0	32.0	38.2
85-89	34.7317432649571	35.0	33.8	35.0	31.8	39.0
90-94	38.58276103567924	39.6	38.0	39.8	35.4	39.8
95-99	39.48554144621191	41.0	39.0	41.0	36.0	41.0
100-104	39.39556099169129	40.0	39.0	41.0	36.0	41.0
105-109	39.22080701114985	40.0	38.4	41.0	35.4	41.0
110-114	39.02260955766405	40.0	38.0	41.0	35.0	41.0
115-119	38.78710152905844	40.0	38.0	41.0	35.0	41.0
120-124	38.513369275907976	40.0	37.4	41.0	34.2	41.0
125-129	38.38329880662694	40.0	36.8	41.0	34.0	41.0
130-134	37.80580448014683	38.8	35.8	40.4	33.8	40.8
135-139	37.99940477348379	39.0	35.4	41.0	34.0	41.0
140-144	37.44373147357784	37.8	35.0	40.6	34.0	41.0
145-149	36.81764973098194	36.6	35.0	39.4	33.6	41.0
150-154	36.16126170282176	35.6	35.0	38.4	33.0	40.4
155-159	35.547074369877464	35.0	35.0	36.8	33.0	39.0
160-164	35.06567031180372	35.0	35.0	36.0	33.0	37.4
165-169	34.758153768731646	35.0	34.2	35.2	33.0	36.4
170-174	34.537872492404766	35.0	34.0	35.0	33.0	36.0
175-179	34.40545909479497	35.0	34.0	35.0	33.0	36.0
180-184	34.16350888209108	34.8	33.8	35.0	32.0	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	103.0
31	1218.0
32	7900.0
33	33754.0
34	101783.0
35	249767.0
36	515260.0
37	832627.0
38	673209.0
39	131443.0
40	7038.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.60452354328563	28.913583785155183	28.70634868431356	20.775543987245626
2	20.815910393625316	28.926739083451576	29.381849760229027	20.87550076269408
3	20.46310565270639	29.066161753789192	29.58822350225363	20.882509091250785
4	20.653074424534005	29.159814948803962	29.442144877420812	20.74496574924122
5	20.76971023889395	28.961624115541106	29.370260568872688	20.898405076692256
6	20.80780579021058	28.83461284270335	29.178138400002506	21.179442967083563
7	21.022832273078933	29.109699526722483	29.000385262307248	20.867082937891333
8	20.905961542677982	29.16674497201367	29.030376210209134	20.896917275099213
9	20.674686700306644	29.062990387235605	29.11631632854418	21.146006583913575
10-14	20.891960546633968	29.03678158759393	28.996838030088046	21.07441983568406
15-19	20.869533895252506	29.055989889213595	28.990691060348368	21.083785155185534
20-24	20.869275487607396	29.0596389183839	28.920466825156687	21.150618768852013
25-29	20.864890388175265	29.117937249227126	28.937114541929382	21.080057820668227
30-34	20.962435358936048	29.016187281332318	28.8295308256829	21.19184653404873
35-39	20.928074972671435	29.00471554799648	28.915087247817628	21.152122231514454
40-44	21.097551235188543	28.95352734266107	28.79264900724481	21.15627241490558
45-49	21.211352395987007	28.891039675753223	28.741672226346303	21.15593570191347
50-54	21.210694631072187	28.8227417520978	28.720255713941174	21.24630790288884
55-59	21.140791447803224	28.921774524451628	28.749134725915624	21.188299301829527
60-64	21.055971095930314	28.89188537350084	28.799547708315714	21.252595822253127
65-69	20.92951579105628	29.020979568568862	28.81021289657743	21.239291743797434
70-74	21.097175369522933	29.204503810338185	28.60130206130995	21.097018758828927
75-79	21.25746641483667	29.146597006229975	28.319175726595315	21.276760852338043
80-84	21.391008353614417	29.045034971167972	28.274118829930185	21.289837845287426
85-89	21.67823236641891	29.11474239106943	28.022265342366637	21.18475990014502
90-94	20.83944132377922	29.358085937692742	28.983895895015323	20.81857684351272
95-99	20.60083561831497	28.942927383574933	29.314392588794348	21.141844409315745
100-104	20.702065671699984	29.01260086227721	29.129411620437985	21.155921845584825
105-109	20.8069264203132	28.99616903695491	28.96877965884289	21.228124883888995
110-114	20.796388638403695	29.018094691170383	28.985022191781823	21.200494478644107
115-119	20.759240206635535	29.06019441186251	28.917210286376342	21.26335509512561
120-124	20.785942546869528	29.132005737401933	28.915629590160247	21.16642212556829
125-129	20.829023552018562	29.07737915286971	28.859192937336775	21.234404357774952
130-134	20.915580616840057	29.069971504463112	28.836735058183177	21.177712820513655
135-139	21.078920293180563	29.028986969909244	28.803353284330818	21.08873945257938
140-144	20.989819997344473	29.086784035053636	28.883003426549596	21.04039254105229
145-149	20.958310775665083	29.09316095132523	28.900054633706958	21.04847363930273
150-154	20.880120113276966	29.25268260948667	28.95977785659548	20.907419420640885
155-159	20.760353018449603	29.301739616630673	29.025991152074333	20.911916212845387
160-164	20.603124711139767	29.52017716461825	29.11785936257153	20.75883876167045
165-169	20.75258346093095	29.81711401911859	28.93017683299727	20.500125686953194
170-174	20.9536919435305	29.851642776714815	28.6821672421085	20.51249803764619
175-179	21.128812459851986	29.82146392162166	28.65480332514872	20.394920293377634
180-184	21.334525888590182	30.04320209374869	28.454643895979537	20.16762812168159
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	10.5
5	33.5
6	95.5
7	217.0
8	430.0
9	649.5
10	821.0
11	1092.0
12	1381.0
13	1622.0
14	1841.0
15	2060.0
16	2419.0
17	3116.0
18	4216.0
19	5931.0
20	8221.5
21	10678.0
22	13139.0
23	15134.5
24	16850.0
25	18787.5
26	21201.5
27	24390.5
28	28253.0
29	33116.5
30	38574.5
31	44553.5
32	51158.5
33	58195.0
34	65908.0
35	74451.5
36	83289.5
37	91844.0
38	99924.5
39	106991.5
40	112662.5
41	116095.5
42	117318.5
43	116469.0
44	113394.0
45	108962.5
46	103394.0
47	96475.0
48	89014.5
49	81328.0
50	73361.0
51	65094.5
52	57229.0
53	50235.0
54	44103.5
55	38411.0
56	33359.0
57	29383.5
58	25830.5
59	22831.0
60	20321.5
61	18237.0
62	16212.0
63	14093.0
64	12256.0
65	10588.0
66	8848.0
67	7258.5
68	5841.0
69	4511.5
70	3339.0
71	2428.0
72	1732.5
73	1176.0
74	805.0
75	504.5
76	299.0
77	163.5
78	91.0
79	54.5
80	29.0
81	16.0
82	9.5
83	2.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	370.0
92-93	823.0
94-95	1384.0
96-97	1922.0
98-99	2455.0
100-101	2736.0
102-103	3263.0
104-105	3712.0
106-107	4207.0
108-109	4522.0
110-111	5074.0
112-113	5454.0
114-115	5794.0
116-117	6210.0
118-119	6566.0
120-121	6847.0
122-123	7130.0
124-125	7468.0
126-127	7826.0
128-129	8099.0
130-131	8451.0
132-133	8901.0
134-135	17475.0
136-137	22049.0
138-139	23961.0
140-141	33545.0
142-143	32153.0
144-145	39186.0
146-147	33204.0
148-149	33012.0
150-151	33847.0
152-153	33414.0
154-155	33428.0
156-157	35400.0
158-159	32889.0
160-161	33429.0
162-163	39044.0
164-165	32860.0
166-167	33355.0
168-169	35104.0
170-171	35697.0
172-173	35956.0
174-175	36241.0
176-177	37747.0
178-179	37480.0
180-181	38561.0
182-183	63054.0
184-185	1582799.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.22107104150597
#Duplication Level	Percentage of total
1	95.2534371642462
2	3.3144127557146774
3	0.503579621101516
4	0.23296708649102096
5	0.14577815036015426
6	0.08918089126680578
7	0.07969198703718706
8	0.049196673312735445
9	0.03614776721797893
>10	0.2956079032517316
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.915267350115735E-5	7.83053470023147E-5	0.0
3	0.0	0.0	0.0	7.83053470023147E-5	1.1745802050347206E-4	0.0
4	0.0	0.0	0.0	7.83053470023147E-5	2.3491604100694412E-4	0.0
5	0.0	0.0	0.0	7.83053470023147E-5	4.3067940851273086E-4	0.0
6	0.0	0.0	0.0	7.83053470023147E-5	4.6983208201388824E-4	0.0
7	0.0	3.915267350115735E-5	0.0	1.1745802050347206E-4	5.872901025173603E-4	0.0
8	0.0	3.915267350115735E-5	0.0	1.1745802050347206E-4	6.65595449519675E-4	0.0
9	0.0	7.83053470023147E-5	0.0	1.9576336750578676E-4	7.047481230208324E-4	0.0
10-14	0.0	7.83053470023147E-5	0.0	2.584076451076385E-4	0.0011510886009340263	0.0
15-19	3.132213880092588E-5	7.83053470023147E-5	1.566106940046294E-5	2.740687145081015E-4	0.001769700842252312	0.0
20-24	1.4094962460416645E-4	7.83053470023147E-5	3.915267350115735E-5	2.8189924920833296E-4	0.0023883130835705983	0.0
25-29	4.2284887381249944E-4	1.0179695110300912E-4	5.48137429016203E-5	3.758656656111106E-4	0.0033044856434976807	0.0
30-34	0.0029756031860879587	1.33119089903935E-4	7.83053470023147E-5	3.9152673501157353E-4	0.004533879591434022	0.0
35-39	0.010774815747518504	1.7227176340509233E-4	7.83053470023147E-5	4.5417101261342535E-4	0.005598832310665501	0.0
40-44	0.01866016419065159	1.9576336750578676E-4	7.83053470023147E-5	5.559679637164344E-4	0.006569818613494203	0.0
45-49	0.02939582726466894	1.9576336750578676E-4	7.83053470023147E-5	5.872901025173603E-4	0.007634771332725684	1.566106940046294E-5
50-54	0.04204214080554276	2.5057711040740705E-4	7.83053470023147E-5	5.872901025173603E-4	0.008668401913156239	4.698320820138882E-5
55-59	0.054570996325913126	3.053908533090274E-4	8.613588170254617E-5	6.577649148194435E-4	0.009936948534593737	7.83053470023147E-5
60-64	0.060780610343196685	3.758656656111106E-4	1.1745802050347207E-4	6.65595449519675E-4	0.01075132414341781	8.613588170254617E-5
65-69	0.06547110062863533	3.9152673501157353E-4	1.1745802050347207E-4	7.204091924212952E-4	0.011667496703344892	1.1745802050347207E-4
70-74	0.11453723106028572	3.9152673501157353E-4	1.33119089903935E-4	7.752229353229156E-4	0.012270447875262715	1.1745802050347207E-4
75-79	1.0523142362253064	4.385099432129624E-4	1.879328328055553E-4	8.378672129247674E-4	0.012802924234878454	1.1745802050347207E-4
80-84	1.5000485493151416	4.698320820138883E-4	1.9576336750578676E-4	9.240030946273136E-4	0.013225773108690955	1.1745802050347207E-4
85-89	1.5014658760958834	4.698320820138883E-4	1.9576336750578676E-4	9.866473722291652E-4	0.013766080003006925	1.1745802050347207E-4
90-94	1.5018574028308949	4.698320820138883E-4	2.1142443690624972E-4	0.0010727832539317115	0.013930521231711786	1.1745802050347207E-4
95-99	1.5023272349129086	4.698320820138883E-4	2.3491604100694415E-4	0.0011432580662337948	0.014416014383126136	1.1745802050347207E-4
100-104	1.5027970669949227	4.698320820138883E-4	2.4274657570717563E-4	0.0011745802050347207	0.01481537165283794	1.1745802050347207E-4
105-109	1.5034626624444423	4.698320820138883E-4	2.740687145081015E-4	0.0011745802050347207	0.015426153359455996	1.1745802050347207E-4
110-114	1.5040812746857608	5.089847555150456E-4	2.8972978390856443E-4	0.0012607160867372667	0.01623269843357984	1.1745802050347207E-4
115-119	1.5045197846289735	5.637984984166659E-4	3.132213880092588E-4	0.0012920382255381926	0.016796496931996505	1.1745802050347207E-4
120-124	1.505271515960196	5.872901025173603E-4	3.132213880092588E-4	0.0012998687602384241	0.01769700842252312	1.1745802050347207E-4
125-129	1.5076128458355653	6.499343801192122E-4	3.2888245740972177E-4	0.00133119089903935	0.018910741301059002	1.1745802050347207E-4
130-134	1.513086389591027	6.890870536203694E-4	3.602045962106477E-4	0.00133119089903935	0.02019494899189696	1.4878015930439796E-4
135-139	1.5191393929143058	7.047481230208324E-4	3.9152673501157353E-4	0.0013703435725405074	0.021181596364126126	1.566106940046294E-4
140-144	1.5243310374205594	7.047481230208324E-4	3.9152673501157353E-4	0.0013703435725405074	0.022160413201655065	1.566106940046294E-4
145-149	1.5288101032690915	7.125786577210639E-4	4.071878044120365E-4	0.0013703435725405074	0.0228416697205752	1.566106940046294E-4
150-154	1.5325844209946031	7.908840047233785E-4	4.5417101261342535E-4	0.001464309988943285	0.023624723190598346	1.566106940046294E-4
155-159	1.5361159921444076	8.222061435243044E-4	5.3247635961574E-4	0.0015661069400462941	0.024345132383019642	1.566106940046294E-4
160-164	1.544345884114351	8.222061435243044E-4	5.794595678171289E-4	0.0015661069400462941	0.025269135477646955	1.566106940046294E-4
165-169	1.5571566388839297	8.613588170254618E-4	5.872901025173603E-4	0.0015895985441469885	0.025864256114864547	1.566106940046294E-4
170-173	1.569483858135769	8.613588170254617E-4	5.872901025173603E-4	0.0016052596135474515	0.026281232087651873	1.566106940046294E-4
>>END_MODULE
