##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478970
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8577824
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.294464889930126	39.0	39.0	39.0	37.0	39.0
2	40.0075781456929	41.0	40.0	41.0	38.0	41.0
3	40.03416938841366	41.0	40.0	41.0	38.0	41.0
4	40.053934307815126	41.0	40.0	41.0	38.0	41.0
5	40.05762953401702	41.0	40.0	41.0	38.0	41.0
6	40.0484772128689	41.0	40.0	41.0	38.0	41.0
7	40.068239217778306	41.0	40.0	41.0	38.0	41.0
8	40.05059628176097	41.0	40.0	41.0	38.0	41.0
9	40.03398495935566	41.0	40.0	41.0	38.0	41.0
10-14	39.96922583163282	41.0	40.0	41.0	38.0	41.0
15-19	39.84909004894482	41.0	40.0	41.0	38.0	41.0
20-24	39.66889425569935	40.8	39.4	41.0	37.2	41.0
25-29	39.44460002909829	40.0	39.0	41.0	36.4	41.0
30-34	39.347635344348404	40.0	38.8	41.0	36.0	41.0
35-39	39.169414061188476	40.0	38.2	41.0	35.0	41.0
40-44	38.639945538635445	39.8	36.8	41.0	35.0	41.0
45-49	37.957969387107966	38.8	35.4	41.0	34.6	41.0
50-54	37.2210377596929	37.2	35.0	39.8	34.0	41.0
55-59	36.49441343165819	35.8	35.0	39.0	34.0	40.8
60-64	35.76116581547954	35.0	34.8	37.0	32.8	39.6
65-69	35.396712476264376	35.0	34.8	36.0	33.0	39.0
70-74	35.17509774040596	35.0	35.0	35.8	33.0	37.4
75-79	35.00147391692811	35.0	35.0	35.0	33.0	37.0
80-84	34.934958562917586	35.0	35.0	35.0	33.0	37.0
85-89	34.771843301984276	35.0	34.6	35.0	32.6	37.2
90-94	39.419497097102905	40.6	39.8	40.6	36.8	40.6
95-99	39.67969395970996	41.0	39.8	41.0	37.0	41.0
100-104	39.57034584808828	41.0	39.0	41.0	36.4	41.0
105-109	39.42309487254038	40.6	39.0	41.0	36.0	41.0
110-114	39.2348467088047	40.0	38.6	41.0	35.0	41.0
115-119	38.978199199524695	40.0	38.0	41.0	35.0	41.0
120-124	38.673619592902455	40.0	37.6	41.0	34.6	41.0
125-129	38.171325801915785	39.6	36.6	40.6	33.8	41.0
130-134	37.89506352506317	39.0	35.4	40.8	34.0	41.0
135-139	37.480263607307876	38.0	35.0	40.6	33.8	41.0
140-144	36.95126630228408	36.6	35.0	39.6	34.0	41.0
145-149	36.25202626007325	35.4	35.0	38.4	33.2	40.8
150-154	35.62954095658293	35.0	35.0	36.8	33.0	39.2
155-159	35.146084810059335	35.0	35.0	36.0	33.0	37.6
160-164	34.80623965327968	35.0	35.0	35.0	33.0	36.4
165-169	34.58372193174436	35.0	34.8	35.0	33.0	36.0
170-174	34.45712414699816	35.0	34.0	35.0	33.0	35.8
175-178	34.16992898651	35.0	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	8.0
29	102.0
30	720.0
31	5007.0
32	24922.0
33	88834.0
34	247260.0
35	591690.0
36	1414884.0
37	2841792.0
38	2691937.0
39	656714.0
40	13954.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.12948178932093	27.04428302562515	27.58307934506467	23.243155839989257
2	22.608974024181425	27.588593564055408	28.10625398702515	21.696178424738022
3	20.96768364564253	26.56252914492067	29.13574584883066	23.33404136060614
4	21.969557780621287	26.193321289874916	29.152253531898065	22.684867397605732
5	22.392870266398564	27.072856705849873	27.920962239374465	22.6133107883771
6	22.823667167803862	26.829484960288298	27.365343471724294	22.981504400183542
7	22.71854726793182	27.357427711270365	27.28671047575702	22.637314545040795
8	22.413155131184787	27.599855161402235	27.557513420653066	22.429476286759904
9	22.993162368451486	27.17585485549715	27.02473261283981	22.806250163211555
10-14	22.70597065176436	27.344261201908548	27.262839619931583	22.686928526395505
15-19	22.864607620767224	27.166073820120346	27.155798486888983	22.813520072223444
20-24	22.754805880838777	27.114976945201953	27.253387339259934	22.876829834699336
25-29	22.820447236968256	27.122715504538213	27.102938927168474	22.953898331325053
30-34	22.9757593534211	27.071933394763054	26.98080072521889	22.971506526596954
35-39	22.871087119530547	27.047337413311347	27.088294187430286	22.99328127972782
40-44	22.92125368858116	27.110544585666478	27.01100652100113	22.95719520475123
45-49	23.153088708744782	26.905721077979685	26.852259967096547	23.088930246178986
50-54	23.07314535714419	26.982358229779486	26.902062807537202	23.04243360553912
55-59	23.046966223601697	26.97732431908139	26.89590273710442	23.079806720212492
60-64	23.141556646534134	26.916241228544674	26.813170799494134	23.129031325427054
65-69	23.027576690778453	26.93321056715549	26.894879167490497	23.14433357457556
70-74	23.014997743017343	26.995359195991895	26.861630641990324	23.128012419000438
75-79	23.072935513715368	26.889322979813997	26.82416659516446	23.21357491130618
80-84	23.001395225642305	26.942986939344994	26.854435343975346	23.201182491037354
85-89	22.990278187102	26.981313675822676	26.799561287338143	23.228846849737184
90-94	21.978152344871045	27.037837499494515	28.216449584865543	22.767560570768893
95-99	22.56069752110613	27.425553740731978	27.286422627828998	22.727326110332893
100-104	22.6135174984683	27.331065196437258	27.27113088201246	22.784286423081976
105-109	22.59425527508669	27.360261342166275	27.24859940282994	22.79688397991709
110-114	22.718724168352296	27.15430817340075	27.166274785069856	22.9606928731771
115-119	22.719983380150833	27.186970273081773	27.069214778409272	23.023831568358126
120-124	22.682573150011166	27.23941043730342	27.07370038275036	23.004316029935055
125-129	22.785930816599826	27.13195691417825	27.012467770257864	23.06964449896406
130-134	22.841912620728063	27.115691373669165	26.962113760380536	23.08028224522224
135-139	22.96832675811558	27.09486339008019	26.88328267222373	23.053527179580502
140-144	23.02976384782944	27.07849039110637	26.837679967108425	23.054065793955765
145-149	22.963651651355754	27.08043540423068	26.899998136835883	23.055914807577683
150-154	22.917482808576455	27.207115267463156	26.911295249739897	22.964106674220496
155-159	22.945677323379762	27.128892243337333	26.888034153894854	23.03739627938805
160-164	22.85329519305818	27.229007802830523	26.942557066359896	22.975139937751397
165-169	22.775465045071247	27.286182655356356	26.9880256855851	22.9503266139873
170-174	22.836217421672284	27.29327289170308	26.932871933776177	22.93763775284846
175-178	22.706450207805553	27.407126028300922	26.997782241583153	22.888641522310373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	0.5
5	0.5
6	1.0
7	2.0
8	5.5
9	18.0
10	31.5
11	64.5
12	142.0
13	243.5
14	428.0
15	717.0
16	1139.0
17	1850.0
18	2917.0
19	4454.5
20	6562.0
21	9608.5
22	13514.5
23	18496.5
24	24775.0
25	32175.0
26	40850.0
27	50854.5
28	61945.0
29	74048.0
30	87450.0
31	101804.0
32	116784.0
33	133217.5
34	150994.5
35	170307.5
36	191198.5
37	212467.5
38	234990.0
39	258868.0
40	282782.5
41	305910.5
42	326903.0
43	344702.0
44	358337.0
45	367005.0
46	370831.5
47	370390.0
48	364980.5
49	353961.0
50	339125.5
51	321808.5
52	301825.0
53	279108.0
54	255630.0
55	231410.5
56	206673.5
57	182748.0
58	160147.0
59	139726.5
60	121032.5
61	104152.5
62	89282.5
63	76514.5
64	65092.5
65	54648.0
66	45699.0
67	37682.0
68	30292.5
69	23783.5
70	18317.5
71	13814.5
72	10160.0
73	7287.5
74	5050.0
75	3383.5
76	2218.0
77	1402.5
78	862.0
79	525.0
80	301.0
81	168.0
82	88.5
83	44.0
84	25.5
85	19.5
86	8.5
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1442.0
92-93	3086.0
94-95	4409.0
96-97	5796.0
98-99	6888.0
100-101	8152.0
102-103	9543.0
104-105	10675.0
106-107	11786.0
108-109	13102.0
110-111	14103.0
112-113	15038.0
114-115	17037.0
116-117	16475.0
118-119	17684.0
120-121	19394.0
122-123	19117.0
124-125	19854.0
126-127	23345.0
128-129	21751.0
130-131	25033.0
132-133	24655.0
134-135	112214.0
136-137	95930.0
138-139	89366.0
140-141	86378.0
142-143	84860.0
144-145	90767.0
146-147	91036.0
148-149	92886.0
150-151	93497.0
152-153	98697.0
154-155	89161.0
156-157	88937.0
158-159	89795.0
160-161	91600.0
162-163	95924.0
164-165	100448.0
166-167	103203.0
168-169	115540.0
170-171	109230.0
172-173	111433.0
174-175	123346.0
176-177	275652.0
178-179	5939559.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.45331713840528
#Duplication Level	Percentage of total
1	87.31257337834923
2	8.362011843376676
3	1.522857250358955
4	0.746586496462218
5	0.4536341065581023
6	0.3483753085685948
7	0.2222317303638968
8	0.17881790235395853
9	0.14953006764990692
>10	0.67989336960261
>50	0.019484430120928355
>100	0.004004116234825297
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.1657968267943011E-5	0.0	1.1657968267943011E-5	2.3315936535886023E-5	0.0
2	0.0013406663508134463	3.4973904803829034E-5	0.0	3.4973904803829034E-5	3.4973904803829034E-5	0.0
3	0.0013406663508134463	4.6631873071772045E-5	0.0	5.828984133971506E-5	4.6631873071772045E-5	0.0
4	0.0013639822873493324	4.6631873071772045E-5	1.1657968267943011E-5	5.828984133971506E-5	5.828984133971506E-5	0.0
5	0.0013639822873493324	4.6631873071772045E-5	1.1657968267943011E-5	5.828984133971506E-5	5.828984133971506E-5	0.0
6	0.0013756402556172754	4.6631873071772045E-5	3.4973904803829034E-5	6.994780960765807E-5	6.994780960765807E-5	0.0
7	0.0013756402556172754	5.828984133971506E-5	3.4973904803829034E-5	6.994780960765807E-5	1.1657968267943013E-4	0.0
8	0.0013756402556172754	5.828984133971506E-5	6.994780960765807E-5	9.326374614354409E-5	1.2823765094737314E-4	0.0
9	0.0013756402556172754	5.828984133971506E-5	6.994780960765807E-5	9.326374614354409E-5	1.6321155575120216E-4	0.0
10-14	0.0014012877858067502	1.0025852710430991E-4	8.626896518277829E-5	1.3523243190813891E-4	2.0984342882297423E-4	0.0
15-19	0.0014479196588785223	1.1657968267943011E-4	1.3056924460096172E-4	1.4689040017608194E-4	2.8678601939139813E-4	0.0
20-24	0.001466572408107231	1.4689040017608194E-4	1.981854605550312E-4	1.6087996209761355E-4	4.7797669898566353E-4	0.0
25-29	0.0014922199382967057	1.7253793036555657E-4	2.3082777170527164E-4	1.981854605550312E-4	6.92483315115815E-4	0.0
30-34	0.0015318570304077117	1.888590859406768E-4	2.5181211458756906E-4	2.4948052093398047E-4	0.0010398907695005166	4.663187307177205E-6
35-39	0.0016507683067407304	2.2383299074450587E-4	2.914492066985753E-4	2.8911761304498666E-4	0.0014922199382967054	2.7979123843063226E-5
40-44	0.0018606117355637049	2.611384892019235E-4	3.5906542265264475E-4	3.1709673688804993E-4	0.002070455164386679	3.730549845741764E-5
45-49	0.0021124238501512736	2.8678601939139813E-4	3.917077338028852E-4	3.3808107977034734E-4	0.002532110707797222	5.129506037894925E-5
50-54	0.0023385884345493682	3.334178924631701E-4	4.1968685764594846E-4	3.5906542265264475E-4	0.003033403343318772	8.860055883636688E-5
55-59	0.0024551681172287985	3.893761401492966E-4	4.5232916879618884E-4	3.963709211100624E-4	0.003476406137500606	1.025901207578985E-4
60-64	0.002541437082411577	4.3134482591389143E-4	4.779766989856635E-4	4.406712005282459E-4	0.003851792715728371	1.1657968267943011E-4
65-69	0.002651021984130241	4.4999757514260026E-4	4.896346672536065E-4	4.779766989856635E-4	0.004276142760681497	1.2590605729378453E-4
70-74	0.003516043229611613	4.639871370641319E-4	4.942978545607837E-4	4.896346672536065E-4	0.0046678504944843826	1.329008382545503E-4
75-79	0.019384869635935643	4.919662609071951E-4	5.059558228287268E-4	5.152821974430811E-4	0.005012926355215495	1.4922199382967055E-4
80-84	0.026412292907851687	5.269401657110242E-4	5.152821974430811E-4	5.595824768612645E-4	0.005257743688842298	1.6087996209761355E-4
85-89	0.026514883028609586	5.735720387827962E-4	5.3393494667179E-4	5.712404451292076E-4	0.005474581898626039	1.6321155575120216E-4
90-94	0.026717731676471797	6.341934737761E-4	5.898931943579165E-4	6.108775372402139E-4	0.005535203333619341	1.6321155575120216E-4
95-99	0.026831979765497636	6.551778166583972E-4	6.295302864689227E-4	6.528462230048087E-4	0.005591161581305468	1.6321155575120216E-4
100-104	0.02693923307356271	7.111360643445236E-4	6.668357849263402E-4	6.831569405014605E-4	0.005698414889370546	1.6321155575120216E-4
105-109	0.027037160007013433	7.437783754947641E-4	6.808253468478718E-4	6.878201278086377E-4	0.005824320946664329	1.6321155575120216E-4
110-114	0.02715607128334645	7.694259056842387E-4	7.414467818411756E-4	7.064728770373466E-4	0.00597121134684041	1.6321155575120216E-4
115-119	0.02723301387391488	8.253841533703652E-4	8.160577787560108E-4	7.297888135732326E-4	0.006230018242388746	1.6321155575120216E-4
120-124	0.02739389383601249	8.510316835598398E-4	8.393737152918969E-4	7.554363437627072E-4	0.006509809480819378	1.6321155575120216E-4
125-129	0.02772498013482207	8.813424010564917E-4	8.440369025990741E-4	8.067314041416563E-4	0.006896854027315086	1.6321155575120216E-4
130-134	0.027988450217677586	8.999951502852005E-4	8.6735283913496E-4	8.440369025990741E-4	0.007302551323039504	1.6321155575120216E-4
135-139	0.028270573049761806	9.09321524899555E-4	8.930003693244348E-4	9.09321524899555E-4	0.007673274713960091	1.8186430497991096E-4
140-144	0.02855969066280679	9.326374614354409E-4	9.303058677818524E-4	9.466270233569725E-4	0.008048661292187856	1.981854605550312E-4
145-149	0.028711244250290046	9.326374614354409E-4	9.326374614354409E-4	9.862641154679786E-4	0.008466016556180216	1.981854605550312E-4
150-154	0.02886046624411972	9.6761136623927E-4	9.396322423962068E-4	0.0010282328012325736	0.008804097635950563	1.981854605550312E-4
155-159	0.029217200073118777	0.0010282328012325738	9.792693345072128E-4	0.0010655382996899916	0.009083888874381194	1.981854605550312E-4
160-164	0.029597249838653722	0.00106786989334358	0.001002585271043099	0.0010841910489187002	0.00942430154780513	1.981854605550312E-4
165-167	0.02988325866016059	0.0011036209960319386	0.001014243239311042	0.0010841910489187002	0.009641139757588872	2.0207144997767888E-4
>>END_MODULE
