##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478971
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8549992
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.196307318182285	39.0	39.0	39.0	37.0	39.0
2	39.940538540854774	41.0	40.0	41.0	38.0	41.0
3	40.00399696280417	41.0	40.0	41.0	38.0	41.0
4	40.02358797528699	41.0	40.0	41.0	38.0	41.0
5	40.03847676114785	41.0	40.0	41.0	38.0	41.0
6	40.03138166678986	41.0	40.0	41.0	38.0	41.0
7	40.05142589607102	41.0	40.0	41.0	38.0	41.0
8	40.03294552790225	41.0	40.0	41.0	38.0	41.0
9	40.01730481151328	41.0	40.0	41.0	38.0	41.0
10-14	39.95624456724637	41.0	40.0	41.0	38.0	41.0
15-19	39.830316309067896	41.0	40.0	41.0	38.0	41.0
20-24	39.65166273839789	40.6	39.4	41.0	37.0	41.0
25-29	39.426441638775806	40.0	39.0	41.0	36.2	41.0
30-34	39.31444930006952	40.0	38.8	41.0	35.6	41.0
35-39	39.12530245642335	40.0	38.2	41.0	35.0	41.0
40-44	38.591227079510716	39.8	36.6	41.0	35.0	41.0
45-49	37.92095702545687	38.8	35.2	40.8	34.6	41.0
50-54	37.19118045958406	37.2	35.0	39.8	34.0	41.0
55-59	36.487274935461926	35.8	35.0	39.0	34.0	40.8
60-64	35.7847730149923	35.0	34.8	37.0	32.8	39.8
65-69	35.43228194833399	35.0	34.8	36.2	33.0	39.0
70-74	35.20530538508106	35.0	35.0	36.0	33.0	37.8
75-79	35.039714306165436	35.0	35.0	35.0	33.0	37.0
80-84	34.96460541717467	35.0	35.0	35.0	33.0	37.0
85-89	34.80601275416398	35.0	34.4	35.0	32.6	37.6
90-94	39.355537758333554	40.6	39.2	40.6	36.6	40.6
95-99	39.61921237347009	41.0	39.2	41.0	36.8	41.0
100-104	39.504160165620405	41.0	39.0	41.0	36.0	41.0
105-109	39.34777391542943	40.2	39.0	41.0	35.6	41.0
110-114	39.15399561467369	40.0	38.2	41.0	35.0	41.0
115-119	38.89394267957469	40.0	38.0	41.0	35.0	41.0
120-124	38.58736281149674	40.0	37.4	41.0	34.2	41.0
125-129	38.0958602218795	39.6	36.2	40.6	33.8	41.0
130-134	37.8207300075063	39.0	35.2	40.8	33.8	41.0
135-139	37.41446083138162	37.8	35.0	40.2	33.6	41.0
140-144	36.893379158073614	36.6	35.0	39.6	33.8	41.0
145-149	36.20843745019102	35.4	35.0	38.4	33.0	40.8
150-154	35.59520631737293	35.0	35.0	36.8	33.0	39.2
155-159	35.112964669273325	35.0	35.0	36.0	33.0	37.6
160-164	34.773756988449044	35.0	35.0	35.0	33.0	36.4
165-169	34.5568730654271	35.0	34.2	35.0	33.0	36.0
170-174	34.42846193596767	35.0	34.0	35.0	33.0	35.8
175-178	34.14008476395657	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	5.0
29	85.0
30	762.0
31	5107.0
32	25352.0
33	91895.0
34	254757.0
35	612605.0
36	1438662.0
37	2829165.0
38	2637041.0
39	640229.0
40	14327.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.154079208495165	27.044317702285568	27.554329875396377	23.24727321382289
2	22.64347147927156	27.556411748689357	28.085570138545158	21.714546633493924
3	20.9633529481665	26.579428378412516	29.11936057951867	23.337858093902312
4	21.95343574590479	26.198235039284246	29.1656296286593	22.68269958615166
5	22.36976362083146	27.10553413383311	27.918400391485747	22.60630185384969
6	22.8375184444617	26.835522185283917	27.35492618004789	22.972033190206496
7	22.727763955802534	27.386680595724535	27.25877404329735	22.626781405175585
8	22.41900343298567	27.609476125825616	27.568224625239417	22.4032958159493
9	22.99091040085184	27.164469861492268	27.004282577106505	22.84033716054939
10-14	22.72575693638076	27.329501594855294	27.264816154213946	22.67992531455
15-19	22.86056408005996	27.15318096204067	27.176397358032617	22.80985759986676
20-24	22.764842353068868	27.116605489221513	27.24020560487074	22.87834655283888
25-29	22.825434222628513	27.120797306009177	27.114827709780315	22.938940761582
30-34	22.98107413433837	27.079087325461828	26.970305937128362	22.969532603071443
35-39	22.88834890137909	27.05405572309307	27.08849552139932	22.96909985412852
40-44	22.925125543977117	27.115400809731753	27.00415158283189	22.955322063459242
45-49	23.151411135823285	26.92295150685521	26.845337399146104	23.0802999581754
50-54	23.06327070247551	26.995725843954006	26.90206493760462	23.038938515965864
55-59	23.04084027213125	27.001807720989678	26.88799474900094	23.06935725787814
60-64	23.130005267841184	26.9248719764884	26.825047321681705	23.12007543398871
65-69	23.031530321899716	26.92095384416734	26.909576055743678	23.137939778189264
70-74	23.00510690536319	27.004722343599852	26.887447380067726	23.102723370969237
75-79	23.066861349110034	26.89795499223859	26.830214577978552	23.204969080672825
80-84	22.994725609099987	26.93829655045291	26.880492987595776	23.186484852851326
85-89	22.97622032862721	26.983557411515708	26.83279235816829	23.207429901688798
90-94	21.98764226683541	27.046767103665648	28.192765756425654	22.772824873073287
95-99	22.55589840541353	27.42409045732202	27.275710738594995	22.74430039866946
100-104	22.613613790824864	27.32355767869059	27.270395798605062	22.79243273187948
105-109	22.60835431191661	27.34838531365315	27.24616479824834	22.797095576181903
110-114	22.725024591613057	27.158272798053222	27.16756337064056	22.94913923969315
115-119	22.714116137564954	27.178270559739552	27.084005713234426	23.02360758946107
120-124	22.680158752933284	27.232565139725896	27.07939247797299	23.00788362936782
125-129	22.781792584193337	27.142306119079297	27.01965935743785	23.056241939289514
130-134	22.849130292555007	27.13366641496662	26.942501729856293	23.074701562622085
135-139	22.95482819600794	27.106260112019864	26.89885748017361	23.04005421179858
140-144	23.032505069870247	27.07868788335353	26.849600435348574	23.039206611427648
145-149	22.946201360077378	27.09370171462383	26.904140199618947	23.05595672567984
150-154	22.912318290813953	27.208839270223862	26.912622872482885	22.966219566479307
155-159	22.937997719825724	27.107474721231316	26.90284019009429	23.051687368848672
160-164	22.85406660205959	27.237800684987302	26.935411040756343	22.972721672196773
165-169	22.753803227087964	27.29160338826022	27.003170771209735	22.951422613442084
170-174	22.83938273354059	27.297512283008547	26.95721622972086	22.905888753730004
175-178	22.692594176006768	27.3965081875007	27.01937528245767	22.89152235403486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	5.5
9	17.0
10	36.0
11	73.5
12	133.0
13	234.0
14	403.0
15	693.0
16	1131.5
17	1815.5
18	2825.0
19	4325.5
20	6443.5
21	9451.0
22	13430.0
23	18353.5
24	24519.5
25	31979.5
26	40644.5
27	50778.0
28	61869.5
29	74063.5
30	87343.0
31	101567.5
32	117180.5
33	133556.0
34	151098.0
35	170315.0
36	190524.0
37	212145.5
38	235065.0
39	258920.5
40	282195.0
41	304590.0
42	325283.0
43	343528.0
44	357891.0
45	365580.5
46	369301.5
47	368748.0
48	362690.0
49	352237.0
50	337610.5
51	320651.0
52	300679.0
53	278146.0
54	254702.0
55	229973.5
56	205398.0
57	181612.0
58	159622.5
59	139439.5
60	120786.0
61	104114.0
62	89547.5
63	76492.0
64	64823.5
65	54735.0
66	45449.0
67	37238.0
68	29975.0
69	23599.5
70	18210.5
71	13704.5
72	10075.0
73	7218.5
74	5057.0
75	3444.0
76	2290.0
77	1445.5
78	885.5
79	540.5
80	316.5
81	165.5
82	79.0
83	40.0
84	21.5
85	12.0
86	5.5
87	3.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1740.0
92-93	3411.0
94-95	4856.0
96-97	6168.0
98-99	7385.0
100-101	10667.0
102-103	10080.0
104-105	12112.0
106-107	13216.0
108-109	14976.0
110-111	15673.0
112-113	16618.0
114-115	18553.0
116-117	18264.0
118-119	18950.0
120-121	20068.0
122-123	22080.0
124-125	22940.0
126-127	22409.0
128-129	24631.0
130-131	24905.0
132-133	27248.0
134-135	126546.0
136-137	104441.0
138-139	95099.0
140-141	94747.0
142-143	106051.0
144-145	105137.0
146-147	100831.0
148-149	106391.0
150-151	102119.0
152-153	96215.0
154-155	102274.0
156-157	103626.0
158-159	109540.0
160-161	101442.0
162-163	99257.0
164-165	104392.0
166-167	106574.0
168-169	115464.0
170-171	114717.0
172-173	119125.0
174-175	128279.0
176-177	278113.0
178-179	5692662.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.47942576462802
#Duplication Level	Percentage of total
1	87.38700634436448
2	8.282637513064847
3	1.4976301681835995
4	0.7171529968583858
5	0.45161466818559054
6	0.35353940885708046
7	0.2367246354885172
8	0.20067295003548574
9	0.16419113226232657
>10	0.6759344288939557
>50	0.029334627241406314
>100	0.003561126564347475
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.84795868814848E-5	0.0	0.0	0.0	2.3391834752593922E-5	0.0
2	0.0012631590766400717	0.0	1.1695917376296961E-5	0.0	7.017550425778176E-5	0.0
3	0.0012631590766400717	0.0	1.1695917376296961E-5	2.3391834752593922E-5	7.017550425778176E-5	0.0
4	0.0012748549940163687	1.1695917376296961E-5	1.1695917376296961E-5	4.6783669505187844E-5	9.356733901037569E-5	0.0
5	0.0012865509113926656	1.1695917376296961E-5	1.1695917376296961E-5	5.84795868814848E-5	1.169591737629696E-4	0.0
6	0.0012865509113926656	1.1695917376296961E-5	1.1695917376296961E-5	5.84795868814848E-5	1.2865509113926655E-4	0.0
7	0.0012865509113926656	1.1695917376296961E-5	2.3391834752593922E-5	7.017550425778176E-5	1.403510085155635E-4	0.0
8	0.0012865509113926656	1.1695917376296961E-5	2.3391834752593922E-5	8.187142163407873E-5	1.403510085155635E-4	0.0
9	0.0012865509113926656	1.1695917376296961E-5	3.508775212889088E-5	8.187142163407873E-5	1.403510085155635E-4	0.0
10-14	0.001288890094867925	4.678366950518784E-5	5.146203645570662E-5	1.0760243986193204E-4	2.105265127733453E-4	0.0
15-19	0.0012982468287689626	8.421060510933812E-5	7.017550425778176E-5	1.3801182504030412E-4	3.2046813611053676E-4	0.0
20-24	0.0013239778469968158	1.450293754660823E-4	1.0526325638667263E-4	1.7076039369393563E-4	4.631583281013596E-4	0.0
25-29	0.0013614047826009663	1.8479549454549198E-4	1.426901919908229E-4	2.1754406319912347E-4	6.549713730726298E-4	0.0
30-34	0.0014690072224628984	2.2222243014964225E-4	1.6374284326815746E-4	2.3859671447645797E-4	0.0010152056282625762	0.0
35-39	0.0017473700560187658	2.3859671447645797E-4	1.9181304497127016E-4	2.80702017031127E-4	0.0013777790669277819	0.0
40-44	0.001969592486168408	2.807020170311271E-4	2.105265127733453E-4	3.0175466830846163E-4	0.001700586386513578	7.017550425778177E-6
45-49	0.0022432769527737572	3.0643303525898036E-4	2.409358979517174E-4	3.3450323696209306E-4	0.0021403528798623435	2.5731018227853317E-5
50-54	0.0024842128507254744	3.812869064672809E-4	2.6432773270431136E-4	3.57895071714687E-4	0.0026619907948451883	3.976611907940966E-5
55-59	0.0026643299783204477	4.678366950518784E-4	2.92397934407424E-4	4.1403547512091244E-4	0.003139184223798104	5.1462036455706634E-5
60-64	0.0027906458859844547	5.099419976065474E-4	3.4385997086313063E-4	4.444448602992845E-4	0.003574272350196351	6.08187703567442E-5
65-69	0.0028982483258463863	5.54386483636476E-4	3.7426935604150275E-4	4.5847996115084086E-4	0.003974272724465707	7.017550425778176E-5
70-74	0.003665500505731467	6.105268870427014E-4	4.1169629164565307E-4	5.07602814131288E-4	0.004491232272498032	7.251468773304115E-5
75-79	0.019302942037840505	6.409362722210733E-4	4.701758785271378E-4	5.473689332106976E-4	0.004921642031945761	8.187142163407873E-5
80-84	0.026500609591213652	6.456146391715923E-4	5.122811810818068E-4	5.894742357653669E-4	0.005167256296847997	8.187142163407873E-5
85-89	0.026566106728520916	6.666672904489268E-4	5.567256671117352E-4	6.26901171369517E-4	0.005398835460898677	8.187142163407873E-5
90-94	0.02670879692051174	7.017550425778176E-4	5.777783183890698E-4	6.713456573994456E-4	0.005440940763453346	8.187142163407873E-5
95-99	0.026793007525621075	7.438603451324867E-4	6.175444374684794E-4	6.994158591025582E-4	0.00549942035033483	8.187142163407873E-5
100-104	0.026881896497680934	7.789480972613776E-4	6.690064739241861E-4	7.298252442809303E-4	0.0056163795240978	8.187142163407873E-5
105-109	0.026942715268037674	8.046791154892308E-4	6.947374921520393E-4	7.555562625087837E-4	0.005721642780484473	8.187142163407873E-5
110-114	0.027033943423572793	8.233925832913059E-4	7.438603451324867E-4	7.602346294593024E-4	0.005864332972475296	8.187142163407873E-5
115-119	0.0271485634138605	8.748546197470126E-4	8.093574824397497E-4	7.976615650634527E-4	0.006072520301773382	8.88889720598569E-5
120-124	0.02740353441266378	8.912289040738285E-4	8.397668676181217E-4	8.608195188954563E-4	0.006493573327320072	9.590652248563508E-5
125-129	0.02771230663139802	9.403517570542757E-4	8.77193803222272E-4	8.865505371233095E-4	0.0069005912520152075	1.1461999028771021E-4
130-134	0.028114646189142632	9.871354265594636E-4	9.122815553511629E-4	9.076031884006441E-4	0.007319305094086637	1.169591737629696E-4
135-139	0.028495933095609917	0.0010362582795399105	9.497084909553131E-4	9.450301240047944E-4	0.007592989560691987	1.169591737629696E-4
140-144	0.02885616735079986	0.001104094600322433	9.84796243084204E-4	9.731003257079072E-4	0.00792749279765408	1.2631590766400716E-4
145-149	0.029073711413998983	0.0011345039855008051	0.0010011705274110198	0.0010058488943615385	0.008306440520646101	1.403510085155635E-4
150-154	0.02924213262421766	0.0011719309211049554	0.0010339190960646511	0.0010222231786883545	0.00869708416101442	1.403510085155635E-4
155-159	0.02954856565947664	0.001204679489758587	0.0010596501142925046	0.0010479541969162075	0.009143868204788963	1.403510085155635E-4
160-164	0.029815232575656214	0.0012397672418874776	0.0010994162333719142	0.0010877203159956174	0.009452640423523204	1.403510085155635E-4
165-167	0.030233946417727642	0.0012709563548909365	0.001111112150748211	0.001095517594246482	0.009692016865824748	1.403510085155635E-4
>>END_MODULE
