##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478972
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7521416
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.2399327467062	39.0	39.0	39.0	37.0	39.0
2	39.98685819265947	41.0	40.0	41.0	38.0	41.0
3	40.04230347051672	41.0	40.0	41.0	38.0	41.0
4	40.06178464267899	41.0	40.0	41.0	38.0	41.0
5	40.06416677391598	41.0	40.0	41.0	38.0	41.0
6	40.058623801688405	41.0	40.0	41.0	38.0	41.0
7	40.08086043904499	41.0	40.0	41.0	38.0	41.0
8	40.05289043977889	41.0	40.0	41.0	38.0	41.0
9	40.0453845392942	41.0	40.0	41.0	38.0	41.0
10-14	39.97994242041658	41.0	40.0	41.0	38.0	41.0
15-19	39.85791303126964	41.0	40.0	41.0	38.0	41.0
20-24	39.67596612127291	40.8	39.4	41.0	37.2	41.0
25-29	39.443577539122955	40.0	39.0	41.0	36.4	41.0
30-34	39.33720219703311	40.0	38.8	41.0	35.8	41.0
35-39	39.153243032960816	40.0	38.2	41.0	35.0	41.0
40-44	38.61915301586829	39.8	36.8	41.0	35.0	41.0
45-49	37.937425452866854	38.8	35.4	41.0	34.6	41.0
50-54	37.21016946808952	37.2	35.0	39.8	34.0	41.0
55-59	36.50225420319791	35.8	35.0	39.0	34.0	41.0
60-64	35.7939749376979	35.0	34.8	37.0	33.0	39.8
65-69	35.44360604439377	35.0	34.8	36.2	33.0	39.0
70-74	35.231411904354175	35.0	35.0	36.0	33.0	38.0
75-79	35.05964504556057	35.0	35.0	35.0	33.0	37.0
80-84	34.98108138148456	35.0	35.0	35.0	33.0	37.0
85-89	34.81266234974903	35.0	34.4	35.0	32.6	37.8
90-94	39.37391308493184	40.6	39.6	40.6	36.6	40.6
95-99	39.641937470682336	41.0	39.4	41.0	37.0	41.0
100-104	39.52113469644335	41.0	39.0	41.0	36.0	41.0
105-109	39.36862615404617	40.2	39.0	41.0	35.8	41.0
110-114	39.168956483268005	40.0	38.2	41.0	35.0	41.0
115-119	38.901887839245184	40.0	38.0	41.0	35.0	41.0
120-124	38.59652453828378	40.0	37.4	41.0	34.4	41.0
125-129	38.10169046687951	39.6	36.2	40.6	33.8	41.0
130-134	37.831980960890824	39.0	35.2	40.8	34.0	41.0
135-139	37.424581383720636	37.8	35.0	40.2	33.6	41.0
140-144	36.89527742073078	36.6	35.0	39.6	34.0	41.0
145-149	36.21368789196934	35.4	35.0	38.4	33.0	40.8
150-154	35.59679143785225	35.0	35.0	36.8	33.0	39.2
155-159	35.11523188445731	35.0	35.0	36.0	33.0	37.6
160-164	34.77418276219437	35.0	35.0	35.0	33.0	36.4
165-169	34.556917851138266	35.0	34.2	35.0	33.0	36.0
170-174	34.42347380660448	35.0	34.0	35.0	33.0	35.8
175-178	34.12994337185032	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	53.0
30	575.0
31	3972.0
32	20092.0
33	74104.0
34	212300.0
35	524836.0
36	1266050.0
37	2506064.0
38	2337221.0
39	563466.0
40	12681.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.164363731510132	27.058814457277723	27.52682473619329	23.249997075018854
2	22.64035123173615	27.577666226678595	28.072945307107066	21.70903723447819
3	20.98102272231718	26.580287541601212	29.133849264553373	23.304840471528234
4	21.969825894485826	26.210915604189424	29.15424436037044	22.66501414095431
5	22.374497036196374	27.118071916245555	27.904719005038416	22.602712042519656
6	22.80393745007589	26.837366793699484	27.383287934080496	22.975407822144128
7	22.728672367011743	27.41676567284671	27.21616514762646	22.63839681251509
8	22.45703468602189	27.57867667471125	27.532435381848313	22.43185325741855
9	23.005548423328797	27.20152694652177	26.95008493081622	22.84283969933321
10-14	22.718868362021194	27.32671348054675	27.260215363702795	22.694202793729264
15-19	22.85351056237283	27.17680553768067	27.168256615509634	22.80142728443687
20-24	22.770379939096575	27.11197731916437	27.234557960894595	22.88308478084446
25-29	22.828834357785823	27.121560621032	27.096038830986082	22.9535661901961
30-34	22.984528445175748	27.061481508269186	26.975045656296633	22.97894439025843
35-39	22.896499808014877	27.039610626509692	27.076460070816456	22.987429494658983
40-44	22.932096828575897	27.104827069796432	27.001362509399822	22.96171359222785
45-49	23.143187399819396	26.924111098229375	26.853525985000697	23.07917551695053
50-54	23.070078825582844	26.982214519180964	26.903769183887714	23.04393747134848
55-59	23.031056918005866	26.999599543490216	26.898365945986768	23.070977592517153
60-64	23.142091861426092	26.921207389672368	26.819245205955898	23.117455542945635
65-69	23.034718462587364	26.913894936804457	26.923603215139273	23.127783385468906
70-74	23.013693166286775	27.00586698036646	26.871160962244346	23.10927889110242
75-79	23.078149114475252	26.899288644585006	26.823380065668484	23.19918217527125
80-84	23.01136115859035	26.926549468876605	26.86393625881084	23.198153113722203
85-89	22.992455144084573	26.980940822845064	26.812999041669812	23.213604991400555
90-94	21.989642325704754	27.051890608814304	28.182123849311314	22.77634321616963
95-99	22.564776107383793	27.41319570473771	27.279181130875823	22.742847057002678
100-104	22.611646829275273	27.33777880839178	27.256663340427025	22.793911021905927
105-109	22.599191963407296	27.347796624880736	27.25467806252847	22.79833334918349
110-114	22.705518002112722	27.15764154586424	27.1686635714602	22.968176880562837
115-119	22.71860357999053	27.18281222043832	27.077832244491844	23.020751955079312
120-124	22.686587696658037	27.24550971491683	27.062570361289858	23.005332227135273
125-129	22.77969835839559	27.127754373053808	27.013888153257103	23.078659115293494
130-134	22.855384998363185	27.138895044398424	26.94115859161439	23.064561365623998
135-139	22.970358047950015	27.100796676315042	26.886035390114344	23.042809885620596
140-144	23.037102045338198	27.080914243541304	26.845915628145423	23.03606808297507
145-149	22.944914726290214	27.087992969072378	26.917710428089052	23.049381876548356
150-154	22.908422401886526	27.20692764637872	26.906708470818693	22.977941480916066
155-159	22.9540994304445	27.12134844151684	26.889004357512103	23.035547770526556
160-164	22.853382751228164	27.244443847068865	26.93664607961075	22.965527322092218
165-169	22.764132448987166	27.298055784361758	27.009029697938775	22.9287820687123
170-174	22.81816917217582	27.316865185124406	26.959043940178773	22.905921702521002
175-178	22.68062658639732	27.432003024935664	27.021594888001065	22.86577550066595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	7.5
9	16.5
10	31.0
11	65.0
12	124.0
13	192.5
14	331.0
15	588.0
16	976.5
17	1558.5
18	2471.5
19	3850.0
20	5707.5
21	8155.5
22	11456.5
23	15799.5
24	21263.5
25	27855.0
26	35432.0
27	43969.5
28	53847.0
29	64844.5
30	76595.0
31	89237.0
32	102367.5
33	116665.5
34	132695.0
35	149387.5
36	167051.0
37	186295.5
38	207019.5
39	228212.5
40	249102.0
41	268927.0
42	286635.0
43	302022.5
44	314469.0
45	322840.5
46	326193.0
47	325072.0
48	319506.0
49	310608.0
50	298549.0
51	282734.5
52	264564.5
53	244814.5
54	224442.5
55	202751.5
56	181064.5
57	159979.5
58	139826.5
59	121863.0
60	105604.0
61	91240.0
62	78420.0
63	67071.0
64	56849.0
65	47804.0
66	39788.5
67	32566.0
68	26105.0
69	20668.0
70	16183.0
71	12189.5
72	8847.5
73	6337.5
74	4465.0
75	3033.5
76	2008.5
77	1268.5
78	781.0
79	464.0
80	262.5
81	144.5
82	82.5
83	43.5
84	21.0
85	10.0
86	3.5
87	3.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1325.0
92-93	2644.0
94-95	4153.0
96-97	5460.0
98-99	7236.0
100-101	9095.0
102-103	8699.0
104-105	11214.0
106-107	11712.0
108-109	12318.0
110-111	12914.0
112-113	14522.0
114-115	16919.0
116-117	15480.0
118-119	16677.0
120-121	17623.0
122-123	17562.0
124-125	18823.0
126-127	19779.0
128-129	23934.0
130-131	23225.0
132-133	24038.0
134-135	103441.0
136-137	91864.0
138-139	97489.0
140-141	83641.0
142-143	88584.0
144-145	85945.0
146-147	90626.0
148-149	94224.0
150-151	92267.0
152-153	85782.0
154-155	84755.0
156-157	100319.0
158-159	89624.0
160-161	85056.0
162-163	86790.0
164-165	94709.0
166-167	93633.0
168-169	93734.0
170-171	103362.0
172-173	107281.0
174-175	108310.0
176-177	251871.0
178-179	5012757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.11238369543779
#Duplication Level	Percentage of total
1	88.39410928703508
2	7.621490105405469
3	1.4910038601178197
4	0.6578157511002183
5	0.4756348673080839
6	0.2961522528975392
7	0.20255300209393343
8	0.16938833749485913
9	0.11998618644460472
>10	0.5553386389619154
>50	0.014688729372883
>100	0.001838981767584432
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.988610655227686E-5	0.0	1.3295368850758953E-5	0.0	2.6590737701517905E-5	0.0
2	0.0012497646719713416	0.0	5.318147540303581E-5	0.0	2.6590737701517905E-5	0.0
3	0.0012497646719713416	1.3295368850758953E-5	5.318147540303581E-5	0.0	3.988610655227686E-5	0.0
4	0.0012497646719713416	2.6590737701517905E-5	7.977221310455372E-5	0.0	5.318147540303581E-5	0.0
5	0.0012497646719713416	3.988610655227686E-5	9.306758195531267E-5	5.318147540303581E-5	7.977221310455372E-5	0.0
6	0.0012497646719713416	3.988610655227686E-5	9.306758195531267E-5	5.318147540303581E-5	7.977221310455372E-5	0.0
7	0.0012497646719713416	6.647684425379477E-5	9.306758195531267E-5	6.647684425379477E-5	1.1965831965683057E-4	0.0
8	0.0012630600408221006	7.977221310455372E-5	9.306758195531267E-5	6.647684425379477E-5	1.4624905735834847E-4	0.0
9	0.0012630600408221006	7.977221310455372E-5	9.306758195531267E-5	6.647684425379477E-5	1.4624905735834847E-4	0.0
10-14	0.001271037262132556	9.306758195531267E-5	1.0370387703591982E-4	9.572665572546446E-5	2.5261200816442014E-4	0.0
15-19	0.001308264294914681	1.3295368850758954E-4	1.2231739342698236E-4	1.64862573749411E-4	4.148155081436793E-4	0.0
20-24	0.0013534685490072613	1.7549886883001816E-4	1.8347609014047355E-4	1.8347609014047355E-4	5.318147540303581E-4	0.0
25-29	0.0014252635408013598	2.074077540718397E-4	2.2070312292259863E-4	2.2070312292259863E-4	7.924039835052335E-4	0.0
30-34	0.0015076948276760652	2.2070312292259863E-4	2.605892294748755E-4	2.472938606241165E-4	0.001180628753947395	0.0
35-39	0.001715102581747905	2.79202745865938E-4	2.7388459832563444E-4	2.632483032450273E-4	0.0016087396309418332	1.3295368850758954E-5
40-44	0.0018613516391062534	3.164297786480631E-4	3.164297786480631E-4	2.8717996717639337E-4	0.002074077540718397	1.3295368850758954E-5
45-49	0.002044827729246727	3.5897495897049174E-4	3.5631588520033996E-4	3.137707048779113E-4	0.0025181428603337458	2.3931663931366114E-5
50-54	0.0022628717783991737	3.8290662290185783E-4	3.8024754913170604E-4	3.5897495897049174E-4	0.0029489128110983358	5.849962294333939E-5
55-59	0.002454325089850103	4.148155081436794E-4	3.93542917982465E-4	4.068382868332239E-4	0.003395637204483837	7.977221310455372E-5
60-64	0.002600574147208451	5.052240163288403E-4	3.9886106552276866E-4	4.8395142616762586E-4	0.003770566606075239	9.572665572546446E-5
65-69	0.0026697100652323977	5.344738278005099E-4	4.387471720750455E-4	5.504282704214207E-4	0.0041508141552069455	1.0636295080607163E-4
70-74	0.003568476999543703	5.903143769736975E-4	4.520425409258044E-4	6.169051146752155E-4	0.004461925786314705	1.0636295080607163E-4
75-79	0.0187863561861224	6.328595572961262E-4	4.520425409258044E-4	6.647684425379476E-4	0.004879400368228536	1.0636295080607163E-4
80-84	0.02543138153773173	6.488139999170369E-4	4.6267883600641156E-4	7.019954753200728E-4	0.005113398860001893	1.0636295080607163E-4
85-89	0.02555369893115871	6.807228851588584E-4	4.812923523974741E-4	7.445406556425014E-4	0.005344738278005099	1.0636295080607163E-4
90-94	0.025729197799988723	6.913591802394655E-4	5.291556802602064E-4	7.950630572753853E-4	0.0054378058599604115	1.0636295080607163E-4
95-99	0.02585151519341571	7.126317704006799E-4	5.903143769736975E-4	8.243128687470552E-4	0.00555480510584709	1.0636295080607163E-4
100-104	0.025941923701600873	7.658132458037157E-4	6.036097458244564E-4	8.615399015291802E-4	0.005695736015665136	1.0636295080607163E-4
105-109	0.026034991283556186	8.056993523559927E-4	6.302004835259744E-4	9.173804507023678E-4	0.0058366669254831804	1.0636295080607163E-4
110-114	0.02608817275895922	8.429263851381177E-4	6.647684425379476E-4	9.492893359441892E-4	0.00601482486808335	1.0636295080607163E-4
115-119	0.026186558488454838	8.748352703799392E-4	6.860410326991619E-4	9.572665572546446E-4	0.006200960031993975	1.0636295080607163E-4
120-124	0.02636737550482516	9.067441556217606E-4	7.36563434332046E-4	9.758800736457071E-4	0.006525367031952495	1.0636295080607163E-4
125-129	0.026659873619541852	9.306758195531267E-4	7.498588031828049E-4	0.001015766180197984	0.006894978286003594	1.0636295080607163E-4
130-134	0.026970985250649615	9.439711884038856E-4	7.950630572753853E-4	0.0010370387703591984	0.007331066384308487	1.0636295080607163E-4
135-139	0.027314005766999196	9.625847047949482E-4	8.349491638276624E-4	0.0010529932129801091	0.007713973007210344	1.0636295080607163E-4
140-144	0.02766766257842938	9.811982211860107E-4	8.562217539888767E-4	0.0010902202457622342	0.008056993523559925	1.0902202457622342E-4
145-149	0.027925592734134103	0.001029061549048743	8.907897130008499E-4	0.0011221291310040556	0.008275037572712373	1.2763554096728596E-4
150-154	0.028079819012802903	0.0010822430244517788	9.14721376932216E-4	0.001154038016245877	0.008535626802187248	1.3295368850758954E-4
155-159	0.028311158430806116	0.0011221291310040556	9.333348933232785E-4	0.001207219491648913	0.008873329170996525	1.3295368850758954E-4
160-164	0.02856111136520038	0.001130106352314511	9.599256310247964E-4	0.0012391283768907345	0.009171145433253525	1.3295368850758954E-4
165-167	0.02882879145806233	0.0011345381419314306	9.705619261054036E-4	0.0012497646719713416	0.009479597990591133	1.4624905735834847E-4
>>END_MODULE
