##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478973
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8570963
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23670945726869	39.0	39.0	39.0	37.0	39.0
2	39.973146891428655	41.0	40.0	41.0	38.0	41.0
3	40.02060433582551	41.0	40.0	41.0	38.0	41.0
4	40.04140561568169	41.0	40.0	41.0	38.0	41.0
5	40.05833276844154	41.0	40.0	41.0	38.0	41.0
6	40.039545031287616	41.0	40.0	41.0	38.0	41.0
7	40.057892327851604	41.0	40.0	41.0	38.0	41.0
8	40.04655731217134	41.0	40.0	41.0	38.0	41.0
9	40.03345038358	41.0	40.0	41.0	38.0	41.0
10-14	39.96980588995659	41.0	40.0	41.0	38.0	41.0
15-19	39.84459498891782	41.0	40.0	41.0	38.0	41.0
20-24	39.66369837321663	40.8	39.4	41.0	37.0	41.0
25-29	39.44235437721525	40.0	39.0	41.0	36.4	41.0
30-34	39.33828754131828	40.0	38.8	41.0	35.8	41.0
35-39	39.15085287382526	40.0	38.2	41.0	35.0	41.0
40-44	38.61893868868644	39.8	36.8	41.0	35.0	41.0
45-49	37.94173245176767	38.8	35.4	41.0	34.6	41.0
50-54	37.208272045976635	37.2	35.0	39.8	34.0	41.0
55-59	36.4893214916457	35.8	35.0	39.0	34.0	40.8
60-64	35.769464154728006	35.0	34.8	37.0	32.8	39.6
65-69	35.41170862597354	35.0	34.8	36.0	33.0	39.0
70-74	35.195655237340304	35.0	35.0	35.8	33.0	37.8
75-79	35.03651412332546	35.0	35.0	35.0	33.0	37.0
80-84	34.963481303092784	35.0	35.0	35.0	33.0	37.0
85-89	34.79228385421801	35.0	34.4	35.0	32.6	37.4
90-94	39.36921182806532	40.6	39.6	40.6	36.6	40.6
95-99	39.63837032478855	41.0	39.2	41.0	37.0	41.0
100-104	39.52108428321874	41.0	39.0	41.0	36.0	41.0
105-109	39.37012461667084	40.2	39.0	41.0	35.8	41.0
110-114	39.17458928203438	40.0	38.2	41.0	35.0	41.0
115-119	38.910049800079186	40.0	38.0	41.0	35.0	41.0
120-124	38.60387304245935	40.0	37.4	41.0	34.4	41.0
125-129	38.10177492794073	39.4	36.2	40.6	33.8	41.0
130-134	37.833858349865125	39.0	35.2	40.8	34.0	41.0
135-139	37.4298709983862	37.8	35.0	40.2	33.6	41.0
140-144	36.90046565551936	36.6	35.0	39.6	34.0	41.0
145-149	36.217030705321505	35.4	35.0	38.4	33.0	40.8
150-154	35.600579951323596	35.0	35.0	36.8	33.0	39.2
155-159	35.123786901552805	35.0	35.0	36.0	33.0	37.6
160-164	34.781048608806714	35.0	35.0	35.0	33.0	36.4
165-169	34.563923725111266	35.0	34.4	35.0	33.0	36.0
170-174	34.43064427098314	35.0	34.0	35.0	33.0	35.8
175-178	34.131665822432936	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	69.0
30	683.0
31	4895.0
32	24051.0
33	88064.0
34	249827.0
35	606487.0
36	1443080.0
37	2844261.0
38	2656981.0
39	639215.0
40	13348.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.1189964301561	27.068358596344428	27.594973866997208	23.21767110650227
2	22.607284619009558	27.58308488789416	28.09762450263757	21.712005990458717
3	20.95305976702968	26.590675983550504	29.12969056102564	23.326573688394177
4	21.963424646682057	26.196367899383066	29.156712028741694	22.68349542519318
5	22.37083510919368	27.10743238536907	27.90369063546302	22.61804186997424
6	22.822639649710307	26.847391594153425	27.385184138585128	22.944784617551143
7	22.72912623704011	27.401460022636897	27.25249193118673	22.61692180913627
8	22.395126428617182	27.60606946967336	27.589676912617637	22.40912718909182
9	22.988606997836765	27.221491914035795	26.995881326287375	22.794019761840065
10-14	22.716070527897507	27.322743080328316	27.283739295106045	22.677447096668136
15-19	22.864924279803798	27.162740056164054	27.177494524244246	22.79484113978791
20-24	22.753713905893655	27.11295801883639	27.270795592047243	22.862532483222715
25-29	22.817529372137063	27.12028975040494	27.114376762564486	22.947804114893508
30-34	22.972459454089346	27.079967560237982	26.99128440993153	22.956288575741137
35-39	22.87863335776855	27.047926819891764	27.09011111120186	22.983328711137826
40-44	22.911129122830186	27.108391437461577	27.035293467023486	22.94518597268475
45-49	23.141334293474376	26.917339393484724	26.873360671373803	23.067965641667104
50-54	23.061429619985528	26.98045248824432	26.917138715917922	23.040979175852236
55-59	23.026577060243987	26.984907063535335	26.911223394617384	23.077292481603294
60-64	23.125420095734867	26.932695894265322	26.821968546591556	23.119915463408255
65-69	23.019039984188474	26.939796613286042	26.905158731871786	23.136004670653694
70-74	22.993497930162572	27.005959540369034	26.89650392843838	23.104038601030013
75-79	23.061301279681174	26.90439335696584	26.84175862152246	23.192546741830526
80-84	22.990487766660525	26.949956498470474	26.872385285060734	23.187170449808264
85-89	22.981970637371788	26.980669500031677	26.81741596597722	23.21994389661932
90-94	21.986899630586425	27.043022131485237	28.20778493667576	22.762293301252573
95-99	22.54443657113697	27.42082295623696	27.294501825642158	22.74023864698391
100-104	22.613089457177118	27.338191545477812	27.27792320484309	22.770795792501975
105-109	22.58009105454436	27.36830651332352	27.2612497391324	22.790352692999726
110-114	22.7277241183745	27.163475822872567	27.15937661670261	22.949423442050325
115-119	22.712561362224	27.188535493273815	27.073957021546953	23.02494612295523
120-124	22.673111280775952	27.234863800401392	27.0944022033895	22.997622715433156
125-129	22.76676282790107	27.146891255407496	27.028173869719506	23.05817204697193
130-134	22.842400429595102	27.148957442225907	26.94726750070837	23.061374627470617
135-139	22.952171431018314	27.11709215013654	26.891716800995475	23.03901961784967
140-144	23.01644148678279	27.080080574183775	26.860650924466512	23.042827014566928
145-149	22.94549241033378	27.103663168872536	26.897629959434365	23.053214461359314
150-154	22.893665698549132	27.199005935171012	26.922975238867743	22.984353127412117
155-159	22.934504557734144	27.1212991034749	26.90342194243257	23.040774396358383
160-164	22.857866001776095	27.233117642708375	26.94750728813015	22.96150906738538
165-169	22.756675902590946	27.282688751706054	27.021157793906266	22.939477551796745
170-174	22.831498016311944	27.317062938496406	26.94706267048637	22.904376374705276
175-178	22.695505564806545	27.417771631073627	27.025530838711454	22.86119196540837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	2.5
8	6.0
9	16.5
10	35.5
11	73.5
12	140.0
13	246.0
14	414.5
15	672.5
16	1111.5
17	1833.5
18	2862.5
19	4335.0
20	6455.5
21	9355.5
22	13317.0
23	18421.0
24	24610.5
25	32134.5
26	40752.5
27	50523.5
28	61941.0
29	74244.5
30	87574.5
31	102315.0
32	117490.5
33	133147.5
34	150667.0
35	170284.5
36	190961.0
37	212951.5
38	236135.5
39	260096.5
40	283689.0
41	306031.5
42	326972.5
43	344810.0
44	358057.0
45	367080.0
46	371227.5
47	370122.0
48	364482.0
49	354174.5
50	339403.5
51	321688.0
52	301492.5
53	278675.5
54	254816.5
55	230083.0
56	205688.0
57	182455.5
58	159722.0
59	138858.0
60	120513.0
61	103989.5
62	89214.5
63	76001.0
64	64571.5
65	54298.0
66	45044.0
67	37076.0
68	29969.0
69	23616.0
70	18138.0
71	13640.0
72	10035.0
73	7228.5
74	5070.0
75	3406.5
76	2147.0
77	1373.5
78	875.5
79	516.5
80	280.0
81	171.0
82	94.5
83	44.0
84	25.5
85	13.5
86	3.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1476.0
92-93	3236.0
94-95	4753.0
96-97	6337.0
98-99	8094.0
100-101	8588.0
102-103	9858.0
104-105	11531.0
106-107	13615.0
108-109	13664.0
110-111	14673.0
112-113	16119.0
114-115	17284.0
116-117	17472.0
118-119	17876.0
120-121	20157.0
122-123	20405.0
124-125	21044.0
126-127	22199.0
128-129	23255.0
130-131	24437.0
132-133	25971.0
134-135	117365.0
136-137	96607.0
138-139	95434.0
140-141	95168.0
142-143	101756.0
144-145	98209.0
146-147	104147.0
148-149	101638.0
150-151	100601.0
152-153	100546.0
154-155	100559.0
156-157	101251.0
158-159	99373.0
160-161	102924.0
162-163	100862.0
164-165	109518.0
166-167	105741.0
168-169	112494.0
170-171	116135.0
172-173	132541.0
174-175	128053.0
176-177	286181.0
178-179	5741816.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.37337829829856
#Duplication Level	Percentage of total
1	87.15839344894086
2	8.458392689476826
3	1.5975950864435928
4	0.7384684429412294
5	0.479184385166218
6	0.3442721546626766
7	0.2542813246069026
8	0.16738729592393065
9	0.13071303023092187
>10	0.6441659872408226
>50	0.02137603192002304
>100	0.005770122446055785
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.166730039553315E-5	0.0	0.0	2.33346007910663E-5	0.0
2	0.0012600684427175803	1.166730039553315E-5	0.0	3.500190118659945E-5	2.33346007910663E-5	0.0
3	0.0012600684427175803	5.833650197766575E-5	0.0	3.500190118659945E-5	3.500190118659945E-5	0.0
4	0.0012834030435086466	8.167110276873205E-5	0.0	3.500190118659945E-5	3.500190118659945E-5	0.0
5	0.0013067376442997128	8.167110276873205E-5	0.0	4.66692015821326E-5	5.833650197766575E-5	0.0
6	0.0013067376442997128	9.33384031642652E-5	0.0	5.833650197766575E-5	1.0500570355979835E-4	0.0
7	0.0013067376442997128	9.33384031642652E-5	0.0	7.00038023731989E-5	1.2834030435086467E-4	0.0
8	0.0013067376442997128	9.33384031642652E-5	1.166730039553315E-5	1.166730039553315E-4	1.2834030435086467E-4	0.0
9	0.0013300722450907791	9.33384031642652E-5	1.166730039553315E-5	1.2834030435086467E-4	1.400076047463978E-4	0.0
10-14	0.0013510733858027387	1.1200608379711825E-4	5.6003041898559125E-5	1.866768063285304E-4	1.866768063285304E-4	0.0
15-19	0.0013650741462773786	1.5634182530014422E-4	7.933764268962543E-5	2.590140687808359E-4	2.706813691763691E-4	0.0
20-24	0.001437411408729684	2.0067756680317017E-4	1.236733841926514E-4	3.1035019052118177E-4	4.573581755048995E-4	0.0
25-29	0.0014934144506282433	2.403463881479829E-4	1.4934144506282434E-4	3.4301863162867466E-4	6.557022822289632E-4	0.0
30-34	0.0015984201541880416	2.5668060870172934E-4	1.8200988617031715E-4	3.546859320242078E-4	9.520517122755052E-4	2.33346007910663E-6
35-39	0.0018364330822569178	3.056832703629685E-4	1.9834410672406357E-4	3.9202129328991383E-4	0.0013114045644579263	1.6334220553746412E-5
40-44	0.002069779090167581	3.476855517868879E-4	2.263456276733431E-4	4.2002281423919336E-4	0.001708092777906053	3.500190118659945E-5
45-49	0.002284457417445391	3.710201525779542E-4	2.7534828933458236E-4	4.4335741503025974E-4	0.0020861133107213273	4.4335741503025974E-5
50-54	0.002480468064090348	4.1302243400187353E-4	3.150171106793951E-4	4.690254759004326E-4	0.002408130801638042	6.066996205677238E-5
55-59	0.0026718117905770917	4.48024335188473E-4	3.3601825139135467E-4	5.11027757324352E-4	0.0027418155929502903	8.167110276873205E-5
60-64	0.002825820155798129	4.970269968497122E-4	3.546859320242078E-4	5.506965786691647E-4	0.0031571714870312706	8.167110276873205E-5
65-69	0.002912158178725075	5.55363498827378E-4	3.8502091305259395E-4	5.74031179460231E-4	0.0035001901186599445	8.867148300605195E-5
70-74	0.003717201906016862	6.253673012005769E-4	4.1768935416008673E-4	5.99699240330404E-4	0.0038852110317125392	1.1433954387622489E-4
75-79	0.019265046413104336	6.673695826244962E-4	4.4569087510936637E-4	6.417015217543233E-4	0.0041838939218381875	1.2834030435086467E-4
80-84	0.026428768855961692	6.907041834155626E-4	4.550247154257929E-4	6.907041834155626E-4	0.004398572249115997	1.3767414466729118E-4
85-89	0.026510439958730425	7.350399249185885E-4	4.736923960586459E-4	7.233726245230554E-4	0.004727590120270032	1.400076047463978E-4
90-94	0.026664448323951463	7.467072253141216E-4	5.133612174034586E-4	7.653749059469746E-4	0.004809261223038765	1.400076047463978E-4
95-99	0.026727451746087344	7.88709506738041E-4	5.670307992229111E-4	7.933764268962543E-4	0.00485359696454179	1.400076047463978E-4
100-104	0.02679978900853965	8.493794687948133E-4	6.160334608841503E-4	8.003768071335743E-4	0.004984270728971762	1.400076047463978E-4
105-109	0.026883793571387482	8.867148300605195E-4	6.370346015961101E-4	8.353787083201735E-4	0.005170947535300292	1.400076047463978E-4
110-114	0.026930462772969614	9.26383651405332E-4	6.790368830200294E-4	8.610467691903465E-4	0.005313288600125797	1.400076047463978E-4
115-119	0.02698413235478907	9.753863130665714E-4	6.953711035737758E-4	8.84381369981413E-4	0.0055909703495394866	1.400076047463978E-4
120-124	0.02715680840064296	9.96387453778531E-4	7.280395446812686E-4	9.123828909306924E-4	0.006003992783541359	1.400076047463978E-4
125-129	0.02741115554926558	0.0010033878340158509	7.63041445867868E-4	9.497182521963985E-4	0.006431015978017873	1.4234106482550443E-4
130-134	0.027756507640973364	0.0010407231952815571	7.700418261051879E-4	9.96387453778531E-4	0.006776368069725654	1.540083652210376E-4
135-139	0.028104193192760252	0.0010827254767054764	7.957098869753609E-4	0.0010523904956770904	0.007175389743252889	1.633422055374641E-4
140-144	0.0284378779840725	0.0011083935375756494	8.237114079246404E-4	0.0010617243359935166	0.00760941331796672	1.7967642609121052E-4
145-149	0.028636222090796564	0.0011410619786831422	8.307117881619603E-4	0.0010640577960726234	0.00801776883181038	1.866768063285304E-4
150-154	0.02876456239514743	0.0011620631193951017	8.470460087157067E-4	0.0010803920166263697	0.00833745286264799	1.866768063285304E-4
155-159	0.02909824718645968	0.001192398100423488	8.633802292694531E-4	0.0011247277581293957	0.008727140695858797	1.866768063285304E-4
160-164	0.029387596236268902	0.0012133992411354478	8.84381369981413E-4	0.0011643965794742085	0.00907249278756658	1.9834410672406357E-4
165-167	0.02961160840386314	0.001248401142322047	8.867148300605194E-4	0.0011745082398170036	0.0093143948157673	1.9834410672406357E-4
>>END_MODULE
